Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 147

3. If the module was powered off by the power switch for a longer period (≥ 1 day),
allow a warm-up period of 1 hour (if a ProFlow flow meter is installed) or 24 hours (if
a Classic flow meter is installed) to achieve optimum reproducibility. You can shorten
the warm-up period if you set the module to standby mode instead of powering it off
completely.
♦ If a Classic flow meter is installed, you can shorten the warm-up period to 1 to
2 hours.
♦ If a ProFlow flow meter is installed, you can shorten the warm-up period to
15 minutes.
4. Solvents will last several weeks. Replace solvents every 2-4 weeks to avoid large
solvent composition changes or bacterial growth. Replace solvents rather than add up
and time the replacements to fit in between sample sets.
5. Particularly when using pre-mixed solvents, make sure that the solvents are always
fresh and that they have been properly prepared. After preparation, degas the solvents
by ultrasonication to remove the excess air that could have introduced from the
mixing.
6. Before running any calibration routines, perform a purge cycle (→ page 72) to ensure
that the pump is properly primed.
7. Flow meter settings:
♦ For Classic flow meters:
Run a viscosity measurement to determine and set the viscosity of unknown solvents
or solvent mixture (→ section 5.5.3.2, page 115). With known solvents, select the
known solvent viscosities from the viscosity list (→ section 4.5.2, page 69) rather
than repeating the test.
Viscosity Measurement functions also as a diagnostics test, providing a Passed,
Limited, or Failed result. With Limited or Failed, follow the instructions provided
by the wizard and repeat the test.
♦ For ProFlow flow meters:
Run a solvent calibration to calibrate the flow meter for unknown solvents
(→ section 5.5.3.1, page 114). With known solvents, select the known solvent type
from the flow meter solvent type list (→ section 4.5.2, page 69) rather than repeating
the calibration procedure.
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 139