Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual
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Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

Solvent racks
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Thermo Scientific Dionex
UltiMate 3000 Series
SRD-3x00 Solvent Racks
Operating Instructions
(Original Operating Instructions)
Revision: 1.8
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc No. 4820.3550

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Summary of Contents for Thermo Scientific Dionex UltiMate 3000 Series

  • Page 1 Thermo Scientific Dionex UltiMate 3000 Series SRD-3x00 Solvent Racks Operating Instructions (Original Operating Instructions) Revision: 1.8 Date: September 2013 © 2013 Thermo Fisher Scientific Inc. Doc No. 4820.3550...
  • Page 2 UltiMate 3000 Series: SRD-3x00 Solvent Racks Operating Instructions...
  • Page 3 SRD-3x00 Solvent Racks Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 Solvent Rack Types: SR-3000, SRD-3200, SRD-3400, SRD-3600 Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: •...
  • Page 4 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: SRD-3x00 Solvent Racks Table of Content Introduction ........................1 1.1 How to Use this Manual .................... 1 1.2 Safety Information ..................... 2 1.2.1 Symbols on the Module and in the Manual ............2 1.2.2 Safety Precautions .................... 3 1.2.3 Consignes de Sécurité...
  • Page 6 UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.4 Special Chromeleon Functions ................35 5.4.1 Leak and Vacuum Monitoring ................ 35 5.4.2 SRD Diagnostics ..................... 35 5.5 Shut Down........................ 36 5.6 Routine and Preventive Maintenance ..............37 Troubleshooting ......................39 6.1 Overview ........................39 6.2 Messages on the Pump Display ................

  • Page 7: Introduction

    The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the Thermo Scientific™ Dionex™ SRD-3x00 Solvent Rack. However, in order to obtain a full understanding of the Solvent Rack, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation.

  • Page 8: Safety Information

    UltiMate 3000 Series: SRD-3x00 Solvent Racks Trademarks Analyst is a registered trademark of AB Sciex. Compass and Hystar are trademarks of Bruker Daltonics. Empower is a trademark of Waters Corp. PEEK is a trademark of Victrex PLC. All other trademarks are property of Thermo Fisher Scientific Inc. and its subsidiaries. 1.2 Safety Information The CE Mark label and cTUVus Mark safety label on the instrument indicate that the instrument is compliant with the related standards.

  • Page 9: Safety Precautions

    UltiMate 3000 Series: SRD-3x00 Solvent Racks Important: Indique que ne pas tenir compte de l'information jointe peut conduire à de faux résultat ou endommager l'instrument. Warning: Indicates that failure to take note of the accompanying information may result in personal injury. Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner des blessures corporelles.
  • Page 10 UltiMate 3000 Series: SRD-3x00 Solvent Racks Dispose of waste substance in an environmentally safe manner that is consistent with all local regulations. Do not allow flammable, toxic, and/or infectious substances to accumulate. Follow a regulated, approved waste disposal program. Never dispose of flammable, toxic, and/or infectious substances through the municipal sewage system.
  • Page 11 UltiMate 3000 Series: SRD-3x00 Solvent Racks ♦ Some capillaries of the RS pumps and some Viper system capillaries are made of ® MP35N , a nickel-cobalt based alloy. Individuals with sensitivity to nickel/cobalt may show an allergic reaction from skin contact. ♦...

  • Page 12: Consignes De Sécurité

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 1.2.3 Consignes de Sécurité Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation de produit chimiques. Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous vous êtes familiarisés avec le contenu de ce manuel. Avertissement: Toutes les personnes utilisant l’instrument doivent observer les consignes de sécurité...
  • Page 13 UltiMate 3000 Series: SRD-3x00 Solvent Racks Gaz dangereux • Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à...
  • Page 14 UltiMate 3000 Series: SRD-3x00 Solvent Racks ♦ Certains capillaires des pompes RS, ainsi que des capillaires du système Viper, sont faits d'alliage de nickel-cobalt MP35N. Contact avec la peau peut provoquer une réaction chez les personnes qui sont sensibles au nickel/cobalt. ♦...

  • Page 15: Intended Use

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 1.3 Intended Use For Research Use Only. Not for use in diagnostic procedures. The device is designed to be operated only be qualified and authorized personnel. All users must know the hazards presented by the device and the used substances. The SRD has been developed as part of the UltiMate 3000 system and is a reliable module for the secure and functional positioning of the solvent reservoirs and for automatic degassing of typical HPLC (high performance liquid chromatography) solvents.

  • Page 16: Federal Communications Commission (Fcc) Note

    UltiMate 3000 Series: SRD-3x00 Solvent Racks Avertissement: Si l'instrument est utilisé de façon non spécifiée par Thermo Fisher Scientific, la protection prévue par l'instrument pourrait être altérée. Thermo Fisher Scientific n'assume aucune responsabilité et ne sera pas responsable des blessures de l'operateur et/ou des dommages de l'instrument.

  • Page 17: Overview

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2 Overview 2.1 Unit Description The SRD is a modern high-quality instrument designed for HPLC analysis, especially as part of the UltiMate 3000 system. The SRD provides a secure location for installation of the solvent reservoirs and, when mounted on top of the UltiMate 3000 system, saves valuable bench space.
  • Page 18 UltiMate 3000 Series: SRD-3x00 Solvent Racks In addition, a solvent rack without degasser channels is available (SR-3000, part no. 5035.9200). The SR-3000 is intended only for use as a solvent organizer, for example, together with a LPG-3400 pump. For this pump type, the degasser is incorporated in the pump.

  • Page 19: Front Panel Elements

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.3 Front Panel Elements Fig. 1: Front panel view Element Description Status LEDs Power The LED is blue when the SRD power is on. Vacuum The LED is green if the degasser is working properly. The Vacuum and Status LEDs are red if the degasser vacuum is insufficient for proper degassing.

  • Page 20: Rear Panel

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.4 Rear Panel Fig. 2: Rear panel view Description 15-pin D-Sub port: Connects the SRD to the pump (→ page 21) DC Input (Power) Only to be used if the SRD is not controlled by the UltiMate 3000 system pump. In this case, connect an external power supply unit (part no.

  • Page 21: Fluid Connections

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.5 Fluid Connections The SRD is designed to provide easy access to the fluid degasser connections. Tilt the front cover upward. The open door locks in its topmost position. Fig. 3: SRD with open front panel door (here SRD-3600) Description Exhaust air outlet Optional: Connect tubing to vent vapors exiting the degassing chambers.

  • Page 22: Leak Sensor

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 2.6 Leak Sensor A leak sensor is installed inside the SRD. If liquid collects in the drip tray under the fluid connections, the leak sensor reports a leak, and the Status LED on the front panel door changes to red.

  • Page 23: Installation

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 3 Installation 3.1 Facility Requirements The installation site must meet the following requirements: • The power connection is on the rear panel. Make sure that ♦ Free and unrestricted access to the power connection is ensured at all times. ♦...
  • Page 24 UltiMate 3000 Series: SRD-3x00 Solvent Racks Important: To prevent the device from falling, grasp the device by the sides, and then lift the unit together with the foam spacers out of the shipping container. Do not lift the device by the foam spacers or the front panel.

  • Page 25: Positioning The Srd In The Ultimate 3000 System

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 3.3 Positioning the SRD in the UltiMate 3000 System If the SRD is part of an UltiMate 3000 system, for example, for analytical HPLC applications, you should stack the individual modules, for example, as shown in Fig. 4 and interconnect them on the rear panel as shown in Fig.
  • Page 26 UltiMate 3000 Series: SRD-3x00 Solvent Racks Solvent Rack Pump (here RS pump) Autosampler Column compartment Detector (here DAD-3000/ MWD-3000) Fig. 5: Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument.

  • Page 27: Connecting The Srd

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 3.4 Connecting the SRD Warning: Never use a connection cord or external power supply provided for the device. Avertissement: Utilisez uniquement le câble de raccordement ou l'alimentation électrique externe spécifique à l’instrument. 3.4.1 Connecting the SRD to an UltiMate 3000 System Pump Locate the connection cable in the accessories kit of the SRD and connect the 15-pin D- Sub port on the rear panel with the Solvent Rack port on the rear panel of the pump.
  • Page 28 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 22 Operating Instructions...

  • Page 29: Preparation For Operation (Startup)

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 4 Preparation for Operation (Startup) 4.1 Overview After you have unpacked, positioned, and connected the SRD described in sections 3.1 through 3.4 (→ page 17 and following pages), prepare the SRD for operation: 1. Connect drain tubing (→ section 4.2). 2.

  • Page 30: Solvent Reservoirs

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 4.3 Solvent Reservoirs All Solvent Racks are shipped with solvent reservoirs and appropriate tubing, including frit holders with filter frits. 4.3.1 General Notes When connecting the solvent reservoirs, observe the following general precautions: • Observe the precautions for solvent selection (→...

  • Page 31: Connecting The Solvent Reservoirs

    UltiMate 3000 Series: SRD-3x00 Solvent Racks • When replacing solvents, make sure that the solvents are miscible. Mix immiscible solvents with an intermediate solvent (for example, isopropanol) to replace them step-by- step. Tip: If the UltiMate 3000 system includes an UltiMate 3000 series autosampler, Thermo Fisher Scientific recommends degassing the wash solution on a continuous basis, for example, by using the degasser of an appropriate SRD.
  • Page 32 UltiMate 3000 Series: SRD-3x00 Solvent Racks When replacing a solvent supply line, remove the filter holder, then the retaining guide, and then the solvent supply line. 7. Connect the lines to the appropriate connection ports on the degassing module. Route the solvent supply lines through the opening between the housing and the front panel door.

  • Page 33: Connecting The Solvent Supply Lines To The Degasser

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 4.3.3 Connecting the Solvent Supply Lines to the Degasser With all UltiMate 3000 system pumps, the solvent supply lines are connected to the pump at the factory. 1. If necessary, route the solvent supply lines through the opening between the front panel door and top cover (→...
  • Page 34 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 28 Operating Instructions...

  • Page 35: Operation And Maintenance

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5 Operation and Maintenance 5.1 Choosing the Solvents Observe the following precautions for solvent selection: • Use only HPLC grade water or better LC-MS grade (0.2 µm, filtered). If water from water purification systems is used that are not properly maintained, polymeric contamination may seriously damage the column, rapidly block solvent frits, and result in early piston seal wear.
  • Page 36 UltiMate 3000 Series: SRD-3x00 Solvent Racks • In an UltiMate 3000 system, some components are made of PEEK. This polymer has superb chemical resistance to most organic solvents. However, it tends to swell when in contact with trichlormethane (CHCl ), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF).

  • Page 37: General Notes For Degasser Operation

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.2 General Notes for Degasser Operation In normal operation, the degasser is quiet. Even if the vacuum pump is running, the operating noise is very low. With higher load, the operating noise may slightly increase. However, this does not impair the degassing performance.

  • Page 38: Operational Settings

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.3 Operational Settings 5.3.1 Turning the Degasser on or off (from the UltiMate 3000 System Pump) When the SRD is connected to the pump, NCS-3500RS, or NCP-3200RS of an UltiMate 3000 system and when the module is powered up, the SRD is powered from the module.

  • Page 39: Turning The Degasser On And Off In Chromeleon

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.3.1.2 Turning the Degasser on and off in Chromeleon If the pump is operated from Chromeleon, select one of the following alternatives to turn the degasser on and off: • Directly from the Panel Tabset (→ page 33) •...

  • Page 40: Turning The Degasser On Or Off (Stand-Alone Operation)

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 3. Verify that the module (pump, NCS, or NCP) is connected to Chromeleon (the LED next to the Connect button is green). If it is not, click Connect. 4. Click More Options and select On or Off under Degasser Mode. To turn the degasser on or off from the Commands dialog box 1.

  • Page 41: Special Chromeleon Functions

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.4 Special Chromeleon Functions This section provides a short overview of some special functions that Chromeleon supports for the SRD. These functions are available in the Commands dialog box for the pump or on the control panel for the pump.

  • Page 42: Shut Down

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.5 Shut Down Observe the following precautions before interrupting the operation or before shipping the SRD: • Thoroughly rinse the degasser with methanol or isopropanol after operation. • For longer periods of inactivity and when using saliferous buffers (which may result in salt crystallization in the gas separation membrane, thereby impairing the degassing performance), rinse with de-ionized water followed by either methanol or isopropanol.

  • Page 43: Routine And Preventive Maintenance

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 5.6 Routine and Preventive Maintenance The SRD is made of high-quality components and materials to minimize maintenance requirements. All surfaces are resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the SRD surface, by using lint- free cloth or paper.
  • Page 44 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 38 Operating Instructions...

  • Page 45: Troubleshooting

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the SRD or UltiMate 3000 system. Status LEDs The status LEDs (light emitting diodes) on the front panel display provide a quick visual check of the operational status of the SRD.

  • Page 46: Messages On The Pump Display

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 6.2 Messages on the Pump Display Each time a fault or error occurs during the operation of the SRD, the Status LED or the Status and Vacuum LEDs on the front panel door change to red. If the SRD is operated from the pump of the UltiMate 3000 system, one or more messages appear on the pump display.

  • Page 47: Operating Problems

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 6.4 Operating Problems The following table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information and remedial actions, see the manuals for the other modules of the UltiMate 3000 system.
  • Page 48 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action High baseline drift The eluents are degraded or Before you start an analysis, be (Cont'd) inhomogeneous. sure that the eluents are already homogenized in the reservoirs. Use fresh solvent and check the eluent filter frits.
  • Page 49 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action Spikes There is electrical interference Isolate the electrical circuit from other modules. from strong current consumers. Consider installing an uninterruptible power supply (UPS). For additional causes, refer to the →...
  • Page 50 UltiMate 3000 Series: SRD-3x00 Solvent Racks Problem Probable Cause Remedial Action RS, SD(N), and BM pumps: Poor peak area precision The gradient is irreproducible. (Cont'd) - Change the gradient. - Check the pump function and degassing. - Check the filter frits in the solvent supply line filters for contamination.

  • Page 51: Service

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 7 Service 7.1 General Notes and Safety Precautions The following sections describe all service and repair procedures that the user may perform. All other maintenance and service procedures must be performed only by Thermo Fisher Scientific service personnel.

  • Page 52: Rinsing The Degassing Channels

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 7.2 Rinsing the Degassing Channels To avoid contamination of the degasser, you should prepare fresh solvents, clean the solvent supply lines, and rinse the degassing channels at regular intervals. Rinsing the degassing channels is especially important for the channel that degasses aqueous solvents (for reverse-phase chromatography).

  • Page 53: Eliminating Leakage In The Solvent Rack

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 7.3 Eliminating Leakage in the Solvent Rack The leak sensor is installed inside the SRD and reports a leak when liquid collects in the drip tray under the fluid connections. Locate the source for the leak, eliminate the cause, and dry the leak sensor.
  • Page 54 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 48 Operating Instructions...

  • Page 55: Technical Information

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 8 Technical Information Bottle capacity: Eight 1-L reservoirs or four 2.5-L reservoirs or two 5-L reservoirs or five 1-L reservoirs and one 5-L reservoir or three 2,5-L reservoirs and two 1-L reservoirs or three 1-L reservoirs and two 2.5-L reservoirs or sixteen 0.5-L reservoirs Degassing channels: SRD-3600: 6 vacuum channels (analytical)
  • Page 56 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 50 Operating Instructions...

  • Page 57: Accessories, Consumables, And Spare Parts

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 9 Accessories, Consumables, and Spare Parts Accessories, consumables, and spare parts are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. 9.1 Standard Accessories The following accessories are shipped with the instrument (subject to change without notice).
  • Page 58 UltiMate 3000 Series: SRD-3x00 Solvent Racks Description Quantity Part No. in the kit SRD-3400 accessories, including: Cable connecting the SRD and the UltiMate 3000 system 6000.1006 module (pump, NCS-3500RS, or NCP-3200RS) Cap (to close the holes in the solvent bottle caps) Included in 6000.0047 Bottle cap for solvent reservoirs...

  • Page 59: Optional Accessories

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 9.2 Optional Accessories Description Part No. Remarks Drain kit for UltiMate 6040.0005 The drain kit includes all required components and detailed 3000 systems installation instructions. To connect the SRD to the power source if the SRD is not External power supply 6510.0004 unit...
  • Page 60 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 54 Operating Instructions...

  • Page 61: Pin Assignment

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 10 Pin Assignment 10.1 15-pin D-Sub Port Signal Name Signal Level Remarks Reserved Solvent Rack Error TTL_high with SRD errors Jumper to pin 9 Solvent Rack Leak TTL high with SRD leaks Reserved V_Degas +24V_supply Supply for the SRD GND_Degas...
  • Page 62 UltiMate 3000 Series: SRD-3x00 Solvent Racks Page 56 Operating Instructions...

  • Page 63: Index

    UltiMate 3000 Series: SRD-3x00 Solvent Racks 11 Index 15-pin D-Sub ............. 21 Leak ..............35 Accessories ..........51, 53 Leak Monitoring ..........35 Leak Sensor ..........16, 47 Chromeleon Diagnostics ............ 35 Maintenance ............37 Error Messages ..........39 Maintenance Intervals ........37 Leak Monitoring ...........
  • Page 64 UltiMate 3000 Series: SRD-3x00 Solvent Racks Shut Down ............36 Site ..............17 Unit Description (Overview) ......11 Solvent Unpacking ............17 Compatibility ..........29 Degasser Compatibility ......... 29 Select ............. 29 Solvent Rack Leak ..........35 Vacuum ............. 35 Solvent Reservoirs ..........

This manual is also suitable for:

Srd-3x00 seriesSrd-3600Srd-3400Srd-3200

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