Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 208

UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
7.6.3.5 Recommended Actions after Main Piston Seal Replacement
Observe the following recommendations when you have replaced the main piston seals in the
pump head:
NC pump
1. Purge both pump heads of the NC pump for 15 minutes with isopropanol
(→ section 4.5.3.1.1, page 73).
2. Afterward, purge the pump heads with the solvent used for the application. Purge the
pump heads for a minimum of 30 minutes to make sure that the isopropanol is
completely removed from the pump.
3. Finally, purge the flow meter (→ page 75) with the solvent used for the application (at
least for 30 minutes).
4. Recommended
Perform flow meter diagnostic tests depending on the flow meter installed. For test
details, see page 134.
♦ For ProFlow flow meter: Run the following diagnostic tests in the given order:
1. Zero Balance Test
2. Detailed Leak Test
♦ For Classic flow meter Run the following diagnostic tests in the given order:
1. Pressure Transducer Test
2. Detailed Leak Test
3. Viscosity Measurement
If Viscosity Measurement fails, the new seals may not yet seal sufficiently tight.
Purge the flow meter for another 30 to 60 minutes (→ page 75) and repeat the test.
Loading pump
• Never run the pump dry. Damage to the pistons or the piston seals could result.
• Allow new piston seals to run in.
a) Connect drain tubing to the purge outlet nozzle.
b) Open the purge valve.
c) Operate the pump for 15 minutes with isopropanol at a flow rate of 1 mL/min. Do not
deliver in circles.
d) Close the purge valve.
e) On the pump outlet, install a flow resistance, for example, a capillary, that can produce
approximately 30 MPa at a flow rate of 1 mL/min.
f) Have the pump deliver isopropanol at 1 mL/min for another 30 minutes.
g) Check the permeability of the filter frit in the inline filter (→ page 209).
h) Remove the capillary from the pump outlet and connect the pump to the system.
Page 200 Operating Instructions