Equilibrating The System - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

4.7 Equilibrating the System

Before using the module for sample analysis, equilibrate the UltiMate 3000 system:
Tip: If the module has not been in use for a longer period (> several days), it will
take some time until the module is ready for operation again:
♦ With a Classic flow meter installed, it will take about 60 minutes until the
module is ready for operation again. The full accuracy will be reached when
the module has been powered on for 24 hours.
♦ With a ProFlow flow meter installed, it will take about 30 minutes until the
module is ready for operation again. The full accuracy will be reached when
the module has been powered on for 1 hour.
1. Pump the starting solvent through the entire system until the system is free of any
other liquid composition.
2. Heat or cool all temperature-controlled devices to the temperature required for the
application.
3. Set the detector wavelengths and turn on the lamps.
4. Monitor the pump pressure. Verify that the reading is correct for the application and is
stable.
Loading pump only
The compression value should remain stable under 100%. The compression value is
displayed, for example, on the Diagnostics menu for the loading pump (→ page 102).
5. Monitor the detector signal and verify that the baseline signal is at the expected
reading for your application and is stable.
Perform system equilibration in Chromeleon or select the required commands and
parameters on the front panel menus of the instruments.
To equilibrate the system from Chromeleon
Select and perform the operating commands and parameters from the Commands dialog
•
box.
• Create and run an equilibration program to automate the process (→ page 92).
To equilibrate the system from the front panel menus
Select and perform the operating commands and parameters on the front panel menus of
the modules. For information about the menus, see section 5.4.2.2 (→ page 98). For
information about the menus of other system modules, see the Operating Instructions for
the respective module.
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 83