Properties Of Common Solvents - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

12.3 Properties of Common Solvents

The table summarizes the properties of the most important solvents in HPLC [1, 2]
UV Transmission at [nm]
20% (0.7 AU)
80% (0.1 AU)
98% (0.01 AU)
Refraction Index (RI) at 20 °C
Boiling Point (BP) in °C at 1013 hPa
Vapor Pressure (VP) at 25 °C
η
Viscosity ( ) at 20 °C (cP = mPa*s)
ρ
Density ( ) (g/mL)
η ρ
/ (cP*mL/g)
Compressibility (χ) at 20 °C (MBar
1)
Critical Flow F (mL/min)
c
Linear Drop in Pressure Δp/l (MPa/m)
3)
Polarity (P')
1)
F = critical flow for 0.25 mm I.D. tubing
C
F (mL/min) = 113 x 0.25 mm x η (cP) / ρ (g/mL)
C
F is an example of a hydrodynamic calculation.
C
Δ
2) p/l = linear drop in pressure for 1 mL/min and 0.25 mm I.D. tubing
Δ -6
p/l (MPa/m) = 6.8 x 10 x 1 mL/min x 100 cm x
Δp/l is an example of a hydrodynamic calculation.
3)
P' is the polarity calculated by L.R. Snyder [3] from experimental measurements by L. Rohrschneider [4]
References
[1] K.K. Unger, E. Weber (Hrsg.), Handbuch der HPLC, GIT Verlag, 1995
[2] D.R. Lide, Handbook of Chemistry and Physics, 79
[3] L.R. Snyder, Journal of Chromatographic Sciences, 16, 223, 1978
[4] L. Rohrschneider, Analytical Chemistry, 45, 1241, 1973
Operating Instructions
190 235
195 245
220 260
1.344 1.424
82 40
118 582
0.37 0.44
0.78 1.32
0.47 0.33
-1
) 99 97
13 9.4
2)
0.06 0.08
5.8 3.1
h
(cP) / (0.25 mm)
NCS-3500RS and NCP-3200RS
200 210
225 230
260 260
1.376 1.378
69 82
202 60
0.33 2.3
0.66 0.78
0.50 2.9
160 100
14 83
0.06 0.40
0.1 3.9
4
th
Edition, CRC Press, 1998-1999
UltiMate 3000 Series:
210 255 --
235 370 --
260 310 < 190
1.329 1.406 1.333
65 66 100
169 216 32
0.60 0.55 1.00
0.79 0.88 0.997
0.76 0.62 1.00
123 93 46
21 18 28
0.10 0.10 0.17
5.1 4.0 10.2
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