Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 172

UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Problem
The degasser of a SRD-3x00
Solvent Rack that is
connected to the module does
not work (the Vacuum and
Status LED on the Solvent
Rack are off).
The system has very high
backpressure.
The loading pump has very
high backpressure.
High baseline drift
Page 164
Probable Cause
The Solvent Rack is not
connected properly to the module
and/or the degasser is not
configured in the Properties
dialog for the module.
The degasser is turned off.
A first generation SRD-3x00
Solvent Rack is connected to the
module.
One or more capillaries in the
system are blocked.
A column switching valve (if
installed) or the injection valve is
blocked.
The column is contaminated or
blocked.
The filter frit on the high-
pressure side of the loading pump
is dirty.
The column is contaminated.
The system is not sufficiently
equilibrated.
The eluents are degraded or
inhomogeneous.
The environmental conditions are
unstable.
For additional causes, refer to the
operating instructions for the
detector.
Remedial Action
In the Chromeleon Server
Configuration program,
verify on the Pumps page of
the Properties dialog for the
module that Degasser
Control is set to External
(→ page 47).
Turn on the degasser.
Contact Service.
Check the capillaries in the
system systematically from
the detector to the pump.
Replace the capillaries if
needed.
Check and replace the valve if
necessary.
Rinse or replace the column.
Replace the filter frit
(→ page 209).
Clean or replace the column.
Flush the system until
equilibration. If equilibration
cannot be reached, purge the
NC pump (→ page 73).
Before you start an analysis,
be sure that the eluents are
already homogenized in the
reservoirs. Use fresh solvent
and check the eluent filter
frits. In aqueous solvents,
growth of microorganisms is
possible.
Make sure that the
temperature and the humidity
are constant. Avoid draft.
Verify on the detector that the
lamp and flow cell covers are
in proper position and that the
front panel door is closed.
→ Detector manual
Operating Instructions