Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 123

2. On the Control menu, select Diagnostics, and then select Solvent Calibration. A
wizard guides you through the procedure. The procedure takes about 60 minutes.
Tip: During solvent calibration, a nanoViper capillary needs to be connected to
the flow meter outlet. A nanoViper capillary with an appropriate inner
diameter (0.25 x 155 mm I.D. x L) is provided in the accessories kit of the
module. Dispose of any liquid that appears at the free end of the capillary
in an appropriate manner or absorb the liquid with a tissue.
3. Solvent calibration includes also diagnostics. Thus, the last wizard page can show the
following results:
♦ Success
Solvent calibration has been successful. The calibration coefficients have been
saved in the flow meter.
♦ Failed or Aborted
The solvent calibration has been failed or aborted. Follow the instructions
provided by the wizard and repeat the procedure.
4. Complete the solvent calibration as instructed in the wizard.
Make sure that you update the solvent or viscosity list in the device configuration by
selecting the Update button on the Flowmeter page.
5.5.3.2 Performing a Solvent Viscosity Measurement (Classic Flow Meter)
Relevant only for NC pump with Classic flow meters
If the viscosity for the solvent that you are using is not yet known (→ page 69), follow the
steps below to establish the viscosity and save the value in Chromeleon.
Tips: It is not possible to calculate the viscosity of premixed solvents from the
viscosities and mixing ratio of the components. Therefore, follow the steps
below to establish the viscosity.
The viscosity values are relative and refer to water at 42 °C.
The steps below refer to Chromeleon 6.80.
If you operate the module from Chromeleon 7.1 and later, establish the
viscosity from the NC_Pump_Diagnostics page of the ePanel set by clicking
Run Viscosity Measurement. The further procedure is similar to the steps
below. For more information, see the Chromeleon Help.
1. In Chromeleon, open a control panel and connect to the timebase in which the module
is installed (→ page 89).
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 115