Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual
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Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

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Thermo Scientific Dionex
UltiMate 3000 Series
Column Compartments
TCC-3000SD and TCC-3000RS
Operating Instructions
(Original Operating Instructions)
Revision: 1.5
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc. No. 4827.3050

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  • Page 1 Thermo Scientific Dionex UltiMate 3000 Series Column Compartments TCC-3000SD and TCC-3000RS Operating Instructions (Original Operating Instructions) Revision: 1.5 Date: September 2013 © 2013 Thermo Fisher Scientific Inc. Doc. No. 4827.3050...
  • Page 2 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Operating Instructions...
  • Page 3 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Column Thermostat Types: TCC-3000SD and TCC-3000RS Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: •...
  • Page 4 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Table of Content Introduction ........................1 1.1 How to Use this Manual .................... 1 1.2 Safety ......................... 3 1.2.1 Symbols on the TCC and in the Manual............3 1.2.2 Safety Precautions .................... 4 1.2.3 Consignes de Sécurité...
  • Page 6 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Preparation for Operation (Startup) ................ 45 4.1 Overview ........................45 4.2 General Precautions for Connecting Capillaries ............46 4.3 Opening the Column Chamber ................48 4.4 Inspecting the Valve Drainage ................. 49 4.5 Installing a Pre-column Heater ................
  • Page 7 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Troubleshooting ......................87 6.1 Overview ........................87 6.2 Messages on the TCC Display ................. 88 6.3 Chromeleon Diagnostics Messages ................. 91 6.4 Operating Problems ....................92 Service .......................... 95 7.1 General Notes and Safety Precautions ..............95 7.2 Removing Gases and Humidity from the Column Chamber ........
  • Page 8 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page iv Operating Instructions...

  • Page 9: Introduction

    The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the Thermo Scientific™ Dionex™ TCC-3000 Thermostatted Column Compartment. However, in order to obtain a full understanding of the TCC, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation.
  • Page 10 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments All rights reserved, including those for photomechanical reproduction and storage on electronic media. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Thermo Fisher Scientific Inc.

  • Page 11: Safety

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 1.2 Safety The CE Mark label and cTUVus Mark safety label on the instrument indicate that the instrument is compliant with the related standards. 1.2.1 Symbols on the TCC and in the Manual The table shows the symbols that are used on the TCC: Symbol Description...

  • Page 12: Safety Precautions

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Warning: Indicates that failure to take note of the accompanying information may result in personal injury. Avertissement: Indique que ne pas tenir compte de l'information jointe peut entraîner des blessures corporelles. 1.2.2 Safety Precautions When working with analytical instrumentation, you must know the potential hazards of using chemical solvents.
  • Page 13 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Dispose of waste substance in an environmentally safe manner that is consistent with all local regulations. Do not allow flammable, toxic, and/or infectious substances to accumulate. Follow a regulated, approved waste disposal program. Never dispose of flammable, toxic, and/or infectious substances through the municipal sewage system.
  • Page 14 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments ® ♦ In an UltiMate 3000 Bio RS system, some system capillaries are made of MP35N nickel-cobalt based alloy. Individuals with sensitivity to nickel/cobalt may show an allergic reaction from skin contact. ♦...
  • Page 15 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • If a leak occurs, turn off the TCC immediately, stop the pump flow, and remedy the situation. • Use only standard solvents (HPLC grade) and buffers that are compatible with all parts that may be exposed to solvents.

  • Page 16: Consignes De Sécurité

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 1.2.3 Consignes de Sécurité Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation de produit chimiques. Veuillez noter : Avant de commencer à utiliser l'instrument, assurez-vous que vous vous êtes familiarisés avec le contenu de ce manuel. Avertissement: Toutes les personnes utilisant l’instrument doivent observer les consignes de sécurité...
  • Page 17 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Gaz dangereux • Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à...
  • Page 18 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments ♦ Dans un système UltiMate 3000 Bio RS, certains capillaires du système Viper sont faits d'alliage de nickel-cobalt MP35N. Contact avec la peau peut provoquer une réaction chez les personnes qui sont sensibles au nickel/cobalt. ♦...
  • Page 19 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • Lorsque vous passez d’une solution saline à un solvant organique, effectuez un rinçage intermédiaire du système HPLC à l'eau dé-ionisée ou qualité HPLC. • Lorsque vous passez à un autre solvant, assurez-vous que le nouveau solvant soit miscible avec celui qui se trouve dans le système HPLC.

  • Page 20: Intended Use

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 1.3 Intended Use For Research Use Only. Not for use in diagnostic procedures. The device is designed to be operated only be qualified and authorized personnel. All users must know the hazards presented by the device and the used substances. The TCC is designed for laboratory research use in high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) applications.

  • Page 21: Federal Communications Commission (Fcc) Note

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments If there is any question regarding appropriate usage, contact Thermo Fisher Scientific before proceeding. Thermo Fisher Scientific cannot be held liable for any damage, material or otherwise, resulting from inappropriate or improper use of the TCC. Warning: If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.
  • Page 22 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 14 Operating Instructions...

  • Page 23: Overview

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2 Overview 2.1 Unit Description The TCC is a modern high-quality instrument designed for HPLC analysis, especially as part of the UltiMate 3000 system. The design has been optimized for minimum dead volume and maximum efficiency.

  • Page 24: Operating Principle

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • If required, the following components can be installed in the TCC: ♦ Pre-column heater (→ page 24) The pre-column heater brings the solvent and the sample to the column temperature before they enter the column. This avoids temperature gradients in the column. ♦...

  • Page 25: Configurations

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.3 Configurations The TCC is available in the following configurations: Column Compartment Part No. TCC-3000RS Column Compartment with post-column thermostatting 5730.0000 Basic version without column switching valves TCC-3000SD Column Compartment without post-column 5730.0010 thermostatting Basic version without column switching valves...

  • Page 26: Front Panel Elements

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.4 Front Panel Elements Fig. 1: Front panel view (example) Element Function Display Shows information about the TCC, for example: - General information upon power-up (→ page 59) - Status screen (→ page 60) - Functions and menus (→...

  • Page 27: Rear Panel

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.5 Rear Panel Fig. 2: Rear panel view Description Power switch (→ page 20) Fuse cartridge (→ page 20) Main power receptacle (→ page 34) Type label Digital I/O ports for communication with external devices (→ page 20) USB (Universal Serial Bus) port for connection to the Chromeleon computer (→...

  • Page 28: Power Switch

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.5.1 Power Switch The main power switch is on the rear panel. The main power switch is used to turn the TCC on or off. 2.5.2 Fuse Cartridge The fuse cartridge contains two slow-blow fuses rated at 2 A. For information about how to change the fuses, see page 108.

  • Page 29: Interior Components

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.6 Interior Components Fig. 3: TCC interior view Description Column switching valve It depends on the TCC configuration whether a valve is installed in this position (→ page 17). Slots for the column ID chip cards (→ page 75) Column switching valve It depends on the TCC configuration whether a valve is installed in this position (→...

  • Page 30: Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.7 Gas and Humidity Sensors A gas sensor and a humidity sensor are installed inside the TCC. The sensors detect any gas or humidity that may accumulate in the column chamber. When a certain concentration of gas or humidity is reached inside the TCC (while the door of the column chamber is closed), the Status LED on the front panel door changes to red and a message appears on the TCC display and in the Chromeleon Audit Trail.
  • Page 31 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • A 2-position, 10-port valve is used for applications that require overlapping injections with offline re-equilibration of the column. Column 2 Column 2 Waste Waste Injector Injector Detector Detector From column re- From column re- equilibration equilibration...

  • Page 32: Column Identification System (Column Id)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.9 Column Identification System (Column ID) The TCC is fitted with an electronic column identification system (column ID) that lets you store column-specific information on a column-ID chip card. This information provides a GLP-compliant overview of the column status. The chip card is connected to the column for the column life cycle.

  • Page 33: Post-Column Cooler

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The decision which pre-column heater is appropriate for the respective application depends on various factors, such as the flow rate and the temperature difference between the ambient temperature and the column temperature. For applications in analytical HPLC and UHPLC, the 2 µL pre-column heater usually provides the best performance.

  • Page 34: Operation From Chromeleon

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.12 Operation from Chromeleon The TCC can be controlled by the Chromeleon Chromatography Management System. To do so, an appropriate Chromeleon version and a Timebase Class 1 Chromeleon license are required. Two modes of software control are available for the TCC: •...

  • Page 35: System Wellness, Predictive Performance, And Diagnostics

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2.13 System Wellness, Predictive Performance, and Diagnostics System Wellness monitors the health of the TCC. Therefore, the TCC supports several performance and reliability features that can help you detect small problems before they turn into big ones: •...
  • Page 36 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 28 Operating Instructions...

  • Page 37: Installation

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3 Installation 3.1 Facility Requirements The installation site must meet the following requirements: • The main power switch and the main power receptacle are on the rear panel. Make sure that ♦ Free and unrestricted access to the main power switch is ensured at all times. ♦...
  • Page 38 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Important: To prevent the TCC from falling, grasp the TCC by the sides, and then lift the TCC together with the foam spacers out of the shipping container. Do not lift the TCC by the foam spacers or the front panel door.

  • Page 39: Positioning The Tcc In The Ultimate 3000 System

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.3 Positioning the TCC in the UltiMate 3000 System If the TCC is part of an UltiMate 3000 system, stack the individual modules (→ Fig. 8) and interconnect them on the rear panel (→ Fig. 9). The arrangement of the system modules depends on the application.
  • Page 40 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Solvent Rack Pump Autosampler Column compartment Detector here DAD-3000 or MWD-3000 Fig. 9: Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument.

  • Page 41: Connecting The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.4 Connecting the TCC 3.4.1 General Information If you want to operate the TCC from Chromeleon Before you connect the TCC to the USB port on the Chromeleon computer and turn on the TCC power, verify that Chromeleon is installed on the computer and that the license code is entered.

  • Page 42: Connecting The Power Cord

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.4.3 Connecting the Power Cord Use the power cord shipped with the pump to connect the instrument to the main power source. Connect the power cord from the main power receptacle on the rear panel (→ Fig. 2, page 19) to a grounded power source.

  • Page 43: Setting Up The Tcc In Chromeleon

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5 Setting Up the TCC in Chromeleon This section provides brief instructions for setting up the TCC in Chromeleon. For details, see the Chromeleon Help. For information about how to set up the TCC in DCMSLink, see section 3.6 (→...
  • Page 44 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments c) Install Chromeleon. d) Turn on the power to the TCC. Windows will now detect the TCC and install the USB software for the TCC automatically. Windows XP will automatically detect the new TCC and launch the Found New Hardware Wizard, which guides you through the USB installation.

  • Page 45: Installing The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5.2 Installing the TCC After the USB software for the TCC has been installed (→ page 35), install and configure the TCC in Chromeleon: 1. Start the Chromeleon Server Monitor (→ page 35) and the Chromeleon server if they are not yet running.

  • Page 46: Configuring The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5.3 Configuring the TCC 3.5.3.1 Initial Installation During the installation, Chromeleon connects to the TCC and transfers the settings from the instrument firmware to Chromeleon, setting the options on the wizard pages accordingly. Verify that the standard settings are correct and make additional settings if needed.
  • Page 47 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Module address • Select the module address of the TCC if necessary. Click Browse and then double-click the TCC that you want to use on the Device List. The address is automatically entered in the Module address box.
  • Page 48 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • Left Valve and Right Valve Indicate which type of column switching valve is installed. Check and change the settings if necessary. Tip: For the TitanHT multi-position valve (pressure < 125 MPa) the entry is 'HT 7 ports, 6 positions (1034 bar/15000 psi)' or 'HT 7 ports, 6 positions (1034 bar;...
  • Page 49 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.5.3.2 Changing the Basic Configuration or Selecting Advanced Settings You may reopen the configuration settings later again to change the basic configuration settings or select advanced settings. 1. Start the Server Configuration program (→ page 37). 2.
  • Page 50 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Configuration Page Use this page to determine whether Chromeleon shall record the temperature signals as separate channels and select the pressure unit. Fig. 12: Configuration page (here TCC-3000RS) Temperature Signal • The check box is selected by default. Accept this setting if you want to record the temperature of the TCC as a separate channel (→...
  • Page 51 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Relays Page The Relays page lists all available relays. Select a check box to enable the relay. If a check box is cleared, the relay will not be available in Chromeleon. You can change the name and timebase assignment for a relay.

  • Page 52: Setting Up The Tcc In Dcmslink

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3.6 Setting Up the TCC in DCMSLink 1. Install and configure the DCMSLink-Software (→ DCMSLink Installation Guide). The Guide is provided on the DCMSLink DVD in the Additional Documents\DCMSLink User Documents folder. 2.

  • Page 53: Preparation For Operation (Startup)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4 Preparation for Operation (Startup) 4.1 Overview After you have unpacked, positioned and connected the TCC (→ sections 3.1 through 3.4, page 29 and following pages), prepare the TCC for operation. When connecting the capillaries, observe the precautions on page 46.

  • Page 54: General Precautions For Connecting Capillaries

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.2 General Precautions for Connecting Capillaries When connecting capillaries, observe the following general precautions: • Observe the precautionary statements for capillaries and capillary connections in section 1.2.2 (→ page 4). • When connecting the capillaries, make sure that the connectors are free from contaminants.
  • Page 55 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments • TCC with column switching valves ♦ To connect the capillaries to the column switching valve, install only Viper fitting connections and/or the ferrules and fitting nuts provided in the accessories kit or original Dionex spare parts.

  • Page 56: Opening The Column Chamber

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.3 Opening the Column Chamber The column chamber in the TCC is easily accessible. The front panel tilts downward when you unlock the door as shown in the picture. Fig. 15: Unlocking the front panel door Important: Do not place any heavy objects on the open front panel door.

  • Page 57: Inspecting The Valve Drainage

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.4 Inspecting the Valve Drainage The column switching valves are fitted with a drip tray for valve drainage. Liquid leaks from the valves are discharged to waste, together with liquid leaks that might have accumulated in the interior of the unit.

  • Page 58: Installing A Separation Column

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.6 Installing a Separation Column Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal or plastic parts inside the column chamber while the temperature is higher than 50 °C.
  • Page 59 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 3. If you want to use column identification Attach the column ID chip card to the column (two chip cards are provided in the TCC accessories kit). Wrap the ribbon around the column, pass the shank of the rivet through a hole, and press down to unite the two parts.

  • Page 60: Installing The Post-Column Cooler Insert

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.7 Installing the Post-Column Cooler Insert Only TCC-3000RS The post-column cooler insert is installed under the column chamber. A removable panel on the front of the instrument provides easy access to the installation section. 1.

  • Page 61: Connecting The Components To The Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.8 Connecting the Components to the Column Switching Valve Warning: Before carrying out any work in the column chamber, wait for the column chamber to cool down. Do not touch any metal or plastic parts inside the column chamber while the temperature is higher than 50 °C.

  • Page 62: Connections On A 2-Position, 6-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments To ensure proper connection, a short part of the capillary must stick out of the ferrule. Therefore, remove the capillary from the valve to verify this. In addition, check that the ferrule grabbed the capillary. When pulling the ferrule gently, you should not be able to move the ferrule on the capillary.

  • Page 63: Connections On A 2-Position, 10-Port Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.8.2 Connections on a 2-position, 10-port Column Switching Valve Fig. 27 shows the connections on a 2-position, 10-port column switching valve for tandem operation. For tandem mode, the UltiMate 3000 system must include one dual gradient pump or two single gradient pumps with identical flow rates, two columns, and one 10-port column switching valve.

  • Page 64: Connections On A Multi-Position Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.8.3 Connections on a Multi-Position Column Switching Valve Fig. 28 shows the connections on a multi-position column switching valve (here as an example, on a 6-position, 7-port valve). Port Connect … Column 1 Column 2 Column 3 Column 4...

  • Page 65: Equilibrating The System

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4.10 Equilibrating the System Before using the TCC for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition.
  • Page 66 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The equilibration panel shows the equilibration status of each instrument in the system. Fig. 30: Equilibration panel To equilibrate the system from the front panel menus Select and perform the operating commands and parameters on the front panel menus of the instruments.

  • Page 67: Operation And Maintenance

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5 Operation and Maintenance The TCC can be controlled by the Chromeleon Chromatography Management System. For details, see section 5.3 (→ page 61). In addition, function keys and menus are available on the front panel display to facilitate operation during, for example, initial installation, diagnostics, and maintenance, allowing you to perform certain actions directly from the TCC: •...

  • Page 68: Status Screen

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.2 Status Screen When the self test was successful, the initial TCC screen changes to the status screen. The TCC type determines the appearance of the screen. Fig. 31 shows the status screen for instruments with column switching valves. For instruments without column switching valves, the appearance of the screen is similar to status screen shown in Fig.

  • Page 69: Operation From Chromeleon

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.3 Operation from Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The TCC is connected to the Chromeleon computer by means of a USB connection. Tip: Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the TCC to the USB port on the Chromeleon computer and turn on the TCC power.

  • Page 70: Direct Control

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments When the TCC is correctly connected to Chromeleon • The Connected LED on the front panel is green. • Front panel input related to the measurement is disabled to prevent changes to the operating parameters.
  • Page 71 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments The commands and parameters available in the dialog box vary, depending on the ♦ Chromeleon version ♦ Options selected for the TCC in the Properties dialog (→ page 38). ♦ Display filter level (Normal, Advanced, or Expert) 5.
  • Page 72 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2. On the Panel Tabset for your timebase, click the Col. Comp. page. Fig. 33: TCC control panel 3. Verify that the TCC is connected to Chromeleon (the LED next to the Connect button is green).

  • Page 73: Automated Control

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.3.3 Automated Control With automated control, you create a program file (PGM) for automated operation of the TCC. You can create programs automatically with the software wizard or manually by editing an existing program. In addition to programs for sample analysis, you can also create programs for special purposes, for example, to ensure that the system automatically restarts operation as desired after a power failure or to automate system shutdown (→...
  • Page 74 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2. Change the program settings as needed. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 34). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.

  • Page 75: Display Screens (Function Keys And Menus)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4 Display Screens (Function Keys and Menus) Function keys and menus are available on the front panel display. Thus, you can make several settings or execute certain commands directly from the TCC. For information about the function keys, see section 5.4.1 and page 69.

  • Page 76: Tcc Menus

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2 TCC Menus Fig. 37 shows an overview of the TCC menus. For information about the general menu layout and structure, see section 5.4.2.1. For information about the commands and parameters that are supported by the menus, see sections 5.4.2.2 through 5.4.2.5 (→...
  • Page 77 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Select an item with the arrow up or down key—the selected item is underlined. Confirm your selection with Select. Back returns you to the previous menu level. The selected menu item or parameter determines which keys appear on the navigation bar: To …...
  • Page 78 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2.2 Main Menu The Main menu provides top-level access to the menu structure. To open the Main menu, show the function keys and select Menu (→ page 67). • Control On the Control menu, you can make the different settings for TCC operation (→...
  • Page 79 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.4.2.4 Information Menu The Information menu provides information for diagnostic purposes (read-only). To … Select … See general information about the TCC, for example, System firmware version, serial number, operating hours, and the last 10 errors. See general information about the switching valves, for example, Valve configuration and switching cycle counter.

  • Page 80: Operational Settings

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5 Operational Settings This section provides specific information about settings and functions that should be considered for operating the TCC. To learn more about ... See page ... Turning on column thermostatting Turning on post-column thermostatting (TCC-3000RS) Activating column identification Selecting the column...

  • Page 81: Turning On Column Thermostatting

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.1 Turning on Column Thermostatting You can turn column thermostatting on and off and set the temperature in Chromeleon or on the instrument display. To turn on column thermostatting from Chromeleon 1. In Chromeleon, open the Commands dialog box for the TCC. 2.

  • Page 82: Turning On Post-Column Thermostatting

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.2 Turning on Post-Column Thermostatting Only TCC-3000RS You can turn post-column thermostatting on and off and set the temperature in Chromeleon or on the front panel display To turn on post-column thermostatting from Chromeleon 1.

  • Page 83: Activating Column Identification (Column Id)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.3 Activating Column Identification (Column ID) 1. Verify that the column ID chip card is attached to the column (→ page 51). 2. Insert the chip card, with the chip facing down, into one of the four chip card readers (A - D).

  • Page 84: Selecting The Column

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Operational column properties • Chromeleon determines and updates the operational information upon each injection. The operational column properties are, for example, the date of the first injection, the date of the last injection, the maximum flow rate while the column is in use, or the total volume of sample that has been injected onto the column.

  • Page 85: Adjusting The Sensitivity Of The Gas And Humidity Sensors

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.5 Adjusting the Sensitivity of the Gas and Humidity Sensors You can adjust the sensitivity of the gas or humidity sensor in Chromeleon or on the front panel display. To adjust the sensitivity from Chromeleon 1.

  • Page 86: Selecting The Status Screen

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.6 Selecting the Status Screen Only TCC with column switching valves As an alternative to the status screen in Fig. 31 (→ page 60), you can select this type of appearance if the instrument is fitted with one or two column switching valves: Compartment Post-Cooler 100.0...

  • Page 87: Smartstartup And Smartshutdown

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.5.8 SmartStartup and SmartShutdown The SmartStartup wizard assists you in automating regular routine tasks (→ page 57). With SmartStartup, the different modules of the UltiMate 3000 system are turned on automatically and in a controlled manner. For example, for the TCC, the column chamber and all components inside are brought to the preferred temperature.

  • Page 88: Special Chromeleon Functions

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.6 Special Chromeleon Functions This section provides a short overview of some special functions that Chromeleon supports for the TCC. To learn more about ... See page ... Equilibration Time and Ready Temp Delta See below.
  • Page 89 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Setting Description Equilibration Time Indicates for how long the preferred temperature must be in the range specified by the temperature set point and the setting for ReadyTempDelta before the TCC is ready for operation and the analysis can be started. Example: Temperature set point: 45 °C, ReadyTempDelta: 1 °C, EquilibrationTime: 0.5 min...

  • Page 90: Predictive Performance

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.6.2 Predictive Performance Predictive Performance provides various functions for estimating the lifetime of consumables and for monitoring and recording service and (re)qualification information. Commands Dialog Box Open the Commands dialog box for the TCC and enter the limits for the predictive performance parameters.

  • Page 91: Tcc Diagnostics

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.6.3 TCC Diagnostics You can check the performance of the TCC gas sensor: 1. On the Control menu, select Diagnostics. The Control menu is visible only when a control panel is open. 2.

  • Page 92: Shutting Down The Tcc

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.7 Shutting Down the TCC Observe the following precautions before interrupting the operation or before shipping the TCC: • For longer periods of inactivity or if saliferous buffers are used, rinse the fluid lines with MS-grade water.
  • Page 93 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments To create a standby or shutdown program Select one of the following alternatives: • Select and perform the operating commands and parameters from the Commands dialog box. • Create and run a corresponding program to automate the process (→ page 65). •...

  • Page 94: Routine And Preventive Maintenance

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5.8 Routine and Preventive Maintenance The TCC is made of high-quality components and materials to minimize maintenance requirements. All surfaces are resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the device surface, by using lint- free cloth or paper.

  • Page 95: Troubleshooting

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the TCC or UltiMate 3000 system. Status LEDs The status LEDs (light emitting diodes) on the front panel provide a quick visual check of the operational status of the TCC.

  • Page 96: Messages On The Tcc Display

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6.2 Messages on the TCC Display Each time a fault or error occurs during the operation of the TCC, the Status LED on the front panel door changes to red and a message appears on the TCC display. In this case, the Prev, Next, and Clear keys appear on the navigation bar.
  • Page 97 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Message Remedial Action (This message may be extended by additional text.) Error while programming flash! Turn the TCC off and on again by pressing the power switch on the rear of the TCC and retry. Flash object error! (This message may be extended by additional text.) Turn the TCC off and on again by pressing the power switch on the...
  • Page 98 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Message Remedial Action Right valve controller no Turn the TCC off and on again by pressing the power switch on the communication. rear of the TCC and retry. Right valve controller Turn the TCC off and on again by pressing the power switch on the configuration error! rear of the TCC and retry.

  • Page 99: Chromeleon Diagnostics Messages

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6.3 Chromeleon Diagnostics Messages If the TCC fails the gas sensor test, perform the instructions given here. If the TCC still fails the test, contact Thermo Fisher Scientific Service for Dionex HPLC Products. Test Result Probable Cause Remedial Action...

  • Page 100: Operating Problems

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 6.4 Operating Problems The table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information and remedial actions, see the manuals for the other modules of the UltiMate 3000 system.
  • Page 101 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Problem Probable Cause Remedial Action The system has very high The column is contaminated or Rinse or replace the column. backpressure at the column blocked. and pump. The temperature does not The ambient temperature is too Reduce the ambient change for some time high or the set point is lower than...
  • Page 102 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Problem Probable Cause Remedial Action Impaired performance of the The post-column cooler insert is Verify that the post-column post-column cooler not installed correctly. cooler is installed correctly. The label must face away from the instrument (→...

  • Page 103: Service

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7 Service 7.1 General Notes and Safety Precautions The following sections describe all service and repair procedures that the user may perform. All other maintenance and service procedures must be performed only by Thermo Fisher Scientific service personnel.
  • Page 104 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments If the original shipping container is not available, appropriate shipping containers and packing material can be ordered from Thermo Fisher Scientific sales organization for Dionex HPLC products. The packing instructions are included in the "Installation and Qualification Documents for Chromatography Instruments"...

  • Page 105: Removing Gases And Humidity From The Column Chamber

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.2 Removing Gases and Humidity from the Column Chamber A gas sensor and a humidity sensor are installed inside the TCC. The sensors detect any gas or humidity that may accumulate in the column chamber. When a certain concentration of gas or humidity is reached inside the TCC (while the door of the column chamber is closed), the corresponding sensor is activated and the Status LED on the front panel door lights red.

  • Page 106: Column Switching Valve

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3 Column Switching Valve The TCC can hold one or two column switching valves (→ page 17). To ensure optimum operation of the valve, observe the following guidelines: • The valve should not be used with concentrated formic acid. Operating, storing, or shipping the valve below 0 °C with water in the fluid passages may •...

  • Page 107: Inspecting The Column Switching Valve For Leakage

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.1 Inspecting the Column Switching Valve for Leakage Several drain holes are provided on the stator ring. If liquid droplets appear on one or more drain holes, this may indicate valve leakage. Drain hole Drain hole Fig.

  • Page 108: Replacing The Valve Pod

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.2 Replacing the Valve Pod Valve pod* for Part No. TitanHP™ column switching valves suitable for pressures < 41 MPa (6000 psi) 2-position, 6-port valve (SST)* 6722.9013 2-position, 10-port valve (SST)* 6722.9023 6-position, 7-port valve (Ti) 6722.9035 The rotor seal in the valve pod is suitable for pH values <...
  • Page 109 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 1. Switch the valve into the appropriate position for the change: In Chromeleon, select ValveLeft or ValveRight and select the position. — or — On the front panel display, show the function keys and select Valve. Select Left valve or Right valve and select the position: 2-position, 6-port or 2-position, 10-port column switching valves Switch the valve into position 1-2.
  • Page 110 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments TitanHT Column Switching Valve 4. Unscrew and remove the locking ring (spanner). Unscrew the spanner using only your hand. Do not use a wrench. Locking ring Fig. 46: Removing the spanner 5. Grasp the pod and pull out firmly to remove the pod from the valve drive body. 6.
  • Page 111 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Troubleshooting Tip If you cannot push the pod completely into the valve drive body, the position of the valve coupling does not match the position of the valve drive. 1. Switch the valve into another position and try again. 2.
  • Page 112 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 5. Push the pod completely into the valve drive body. Be sure to install the pod in the correct orientation. Align the large slot in the pod with the matching runner in the valve drive body. Runner in the valve drive body Large slot in the pod Fig.

  • Page 113: Replacing The Valve Stator

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.3 Replacing the Valve Stator Stator for Part No. TitanHP™ column switching valves suitable for pressures < 41 MPa (6000 psi) 2-position, 6-port switching valve (SST) 6722.9011 2-position, 10-port switching valve (SST) 6722.9021 6-position, 7-port switching valve (Ti) 6722.9031...
  • Page 114 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 2. Remove the stator from the valve body. 3. Mount the replacement stator on the valve so that the stator locating pins in the valve body enter the matching holes in the stator. 4.

  • Page 115: Rotor Seal And Stator Face Seal

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.3.4 Rotor Seal and Stator Face Seal The valve type and the pH value of the application determine the type of seals: Description Part No. Rotor Seal kit for TitanHT™ valves, for pressures < 41 MPa (6000 psi) and pH values < 10 2-position, 6-port switching valve (SST) 6722.9012 2-position, 10-port switching valve (SST)
  • Page 116 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Observe the following precautions when replacing the seals: • To avoid scratches on the valve surfaces, do not use pointed or sharp-edged tools to remove or install a seal. Even minute scratches may result in poor sealing performance of the valve.

  • Page 117: Replacing The Main Power Fuses

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.4 Replacing the Main Power Fuses Warning: Turn off the TCC. Disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez l'instrument. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur. 1.

  • Page 118: Updating The Tcc Firmware

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 7.5 Updating the TCC Firmware The TCC is shipped with the most recent firmware version. The TCC firmware is also included in Chromeleon. To check which firmware version is installed in the TCC and which version is included in Chromeleon: •...
  • Page 119 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 4. On the General page (→ page 38), click Firmware Download. A message displays the firmware version that is currently installed in the TCC and the version that will be downloaded from Chromeleon. Tip: If the TCC comes with a newer firmware than the version included in Chromeleon, do not downgrade the firmware.
  • Page 120 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 112 Operating Instructions...

  • Page 121: Technical Information

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 8 Technical Information Temperature range: TCC-3000RS 5 °C to 110 °C, max. 18 °C below ambient TCC-3000SD 5 °C to 80 °C, max. 18 °C below ambient ±0.5 °C Temperature Accuracy (Column Chamber): Temperature Stability ±0.1 °C (Column chamber):...
  • Page 122 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments GLP features: Electronic identification system for 4 columns In Chromeleon: Full support of automatic equipment qualification (AutoQ™ routines) and System Wellness monitoring All system parameters are recorded in the Chromeleon Audit Trail. Power requirements: 100 - 120 V, 60 Hz;...

  • Page 123: Accessories, Consumables, And Spare Parts

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 9 Accessories, Consumables, and Spare Parts Accessories, spare parts, and consumables for the TCC are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. 9.1 Standard Accessories The following standard accessories are shipped with the instrument (subject to change without notice).
  • Page 124 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Description Part No. Qty in the accessories kit Accessories of TCC-3000RS, including: Fuse, 6.3A, slow-blow (5 x 20 mm) Included in 6710.9001 Fuse, 2A, slow-blow (5 x 20 mm) Included in 6710.9001 Column ID Included in 6710.1505...

  • Page 125: Optional Accessories

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 9.2 Optional Accessories Column Switching Valves Part No. To fit the TCC with column switching valves, you need the appropriate valve drive (actuator) and pod. Valve Drive (Actuator) for TitanHP™ column switching valve, suitable for pressures <...
  • Page 126 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Column Switching Valves Part No. TitanHT™ column switching valve, Pods for suitable for pressures < 125 MPa (18130 psi) (Cont'd) 6-position, 7-port switching valve (SST) 6730.0050 The rotor seal in the valve pod is suitable for pH values 0 - 10.
  • Page 127 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Capillary kits (Viper) for UltiMate 3000 Part No. RSLC Systems Kit for UltiMate 3000 RSLC systems with 6040.2301 (Single stack setup) LPG-3400RS or DGP-3600RS The kit includes 3 Viper capillaries (SST; one each of 0.13 x 250 mm, 0.13 x 350 mm, and 0.18 x 450 mm I.D.

  • Page 128: Consumables And Spare Parts

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 9.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products.
  • Page 129 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Description Part No. Capillaries, kit of capillaries for UltiMate 3000 SD systems with 6040.2309 HPG-3200SD or HPG-3400SD The kit includes 3 Viper capillaries, SST (one each of 0.18 x 250 mm, 0.18 x 350 mm, and 0.18 x 550 mm I.D. x L). Capillary (0.25 x 1.58 mm) (I.D.
  • Page 130 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Description Part No. Pod for TitanHP™ column switching valves, suitable for pressures < 41 MPa (6000 psi) 6722.9013 2-position, 6-port switching valve (SST)* 6722.9023 2-position, 10-port switching valve (SST)* 6722.9035 6-position, 7-port switching valve (Ti)* The rotor seal in the valve pod is suitable for pH values <...
  • Page 131 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Description Part No. Rotor Seal kit for biocompatible TitanHT™ column switching valves, suitable for pressures < 103 MPa (15000 psi) and pH values 0 - 14 TitanHT™ column switching valve (6 positions, 7 ports) suitable for pressures <...
  • Page 132 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 124 Operating Instructions...

  • Page 133: Appendix - Digital I/O (Pin Assignment)

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 10 Appendix - Digital I/O (Pin Assignment) The two digital I/O ports provide two digital inputs and two relay outputs that can be used to exchange digital signals with external devices. The port numbering on the rear panel corresponds to the numbering of the relays and digital inputs in Chromeleon.
  • Page 134 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 126 Operating Instructions...

  • Page 135: Index

    UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments 11 Index Configuration............. 17 Dialog Box ............ 42 Menu ............. 71 Accessories Connection Optional ............117 Chromeleon Computer ........33 Standard ............115 Digital I/O ..........34, 125 Power Cord ........... 34 Signal Cable ..........
  • Page 136 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Front Panel Elements ......... 18 Messages ............88 Function Key ..........67, 68 Module Address ..........39 Arrow Down ..........69 Arrow Right ..........69 Arrow Up ............69 OK ..............69 Back ..............
  • Page 137 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Safety ..............3 TCC Display ............18 Safety Precautions ......... 4, 95 TCC Drainage ............ 56 Select ..............69 Technical Information ........113 Sensitivity (Sensor) ..........77 Temperature..........70, 71 Server Configuration Program ..... 37, 44 Temperature Signal ...........
  • Page 138 UltiMate 3000 Series: TCC-3000SD and TCC-3000RS Column Compartments Page 130 Operating Instructions...

This manual is also suitable for:

Tcc-3000sdTcc-3000rs

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