Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual
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Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

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Thermo Scientific Dionex
UltiMate 3000 Series
LPG-3400XRS Pump
Operating Instructions
(Original Operating Instructions)
Revision: 1.1
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc. No. 4820.4301

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  • Page 1 Thermo Scientific Dionex UltiMate 3000 Series LPG-3400XRS Pump Operating Instructions (Original Operating Instructions) Revision: 1.1 Date: September 2013 © 2013 Thermo Fisher Scientific Inc. Doc. No. 4820.4301...
  • Page 2 UltiMate 3000 Series: LPG-3400XRS Pump Operating Instructions...
  • Page 3 UltiMate 3000 Series: LPG-3400XRS Pump Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BM HPG-3200SD, HPG-3200RS, HPG-3200BX HPG-3400SD, HPG-3400RS LPG-3400SD(N), LPG-3400RS, LPG-3400XRS DGP-3600SD(N), DGP-3600RS Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: •...
  • Page 4 UltiMate 3000 Series: LPG-3400XRS Pump Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: LPG-3400XRS Pump Table of Content Introduction ........................1 1.1 How to Use this Manual .................... 1 1.2 Safety ......................... 3 1.2.1 Symbols on the Pump and in the Manual ............3 1.2.2 Safety Precautions .................... 4 1.2.3 Consignes de Sécurité...
  • Page 6 UltiMate 3000 Series: LPG-3400XRS Pump 3.5.2 Installing the Pump in Chromeleon ..............47 3.5.3 Configuring the Pump in Chromeleon ............48 3.6 Setting Up the Pump in DCMSLink ................ 52 3.7 When a Corona or Coulochem III Detector is connected ........53 Preparation for Operation (Startup) ................
  • Page 7 UltiMate 3000 Series: LPG-3400XRS Pump Service ........................103 7.1 General Notes and Safety Precautions ..............103 7.2 Eliminating Leakage ....................105 7.3 Pump Head, Pistons and Piston Seals ..............106 7.3.1 Visually Inspecting the Pump for Piston Seal Leakage ........ 109 7.3.2 Pump Head and Pistons ................
  • Page 8 UltiMate 3000 Series: LPG-3400XRS Pump Page iv Operating Instructions...

  • Page 9: Introduction

    The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the Thermo Scientific™ Dionex™ pump. However, in order to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation.
  • Page 10 UltiMate 3000 Series: LPG-3400XRS Pump Trademarks Analyst is a registered trademark of AB Sciex. Compass and Hystar are trademarks of Bruker Daltonics. Luer-Lok is a trademark of Beckton, Dickinson and Company. Empower is a trademark of Waters Corp. PEEK is a trademark of Victrex PLC. Microsoft and Windows are registered trademarks of Microsoft Corp.

  • Page 11: Safety

    UltiMate 3000 Series: LPG-3400XRS Pump 1.2 Safety The CE Mark label and cTUVus Mark safety label on the rear panel indicate that the pump is in compliance with the related standards. 1.2.1 Symbols on the Pump and in the Manual The table shows the symbols used on the pump: Symbol Description...

  • Page 12: Safety Precautions

    UltiMate 3000 Series: LPG-3400XRS Pump 1.2.2 Safety Precautions When working with analytical instrumentation, you must know the potential hazards of using chemical solvents. Wear appropriate protective clothing. Tip: Before initial operation of the pump, make yourself familiar with the contents of this manual. For the safety precautions in French, see page 8.
  • Page 13 UltiMate 3000 Series: LPG-3400XRS Pump Hazardous gases • Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample includes volatile or flammable solvents, do not allow them to enter the workspace. If the mobile phase or sample includes volatile or flammable solvents, avoid open flames and sparks.
  • Page 14 UltiMate 3000 Series: LPG-3400XRS Pump • Replace faulty communication cables. • Replace faulty power cords. Never use a power cord other than the power cords provided for the device. • Use only the original spare parts and accessories authorized for the device by Thermo Fisher Scientific.
  • Page 15 UltiMate 3000 Series: LPG-3400XRS Pump • Thermo Fisher Scientific advises against recycling the solvents. This may impair the performance of the seals. • If the pump flow is interrupted for longer periods (> 1 hour), turn off the lamps in any UV or RF detector connected to the pump.

  • Page 16: Consignes De Sécurité

    UltiMate 3000 Series: LPG-3400XRS Pump 1.2.3 Consignes de Sécurité Si vous utilisez d'instrumentation analytique, vous devez connaître les risques d'utilisation de produit chimiques. Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous vous êtes familiarisés avec le contenu de ce manuel. Avertissement: Toutes les personnes utilisant l’instrument doivent observer les consignes de sécurité...
  • Page 17 UltiMate 3000 Series: LPG-3400XRS Pump Gaz dangereux • Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, vous devez assurer qu'ils ne pénètrent dans l'espace de travail. Si la phase mobile ou l’échantillon contient des solvants volatils ou inflammables, évitez les flammes nues et les sources d’étincelles à...
  • Page 18 UltiMate 3000 Series: LPG-3400XRS Pump • Quand les capots de protection de l’appareil sont démontés, vous êtes exposés à des connexions électriques sous haute tension deviennent accessibles. Débranchez l'instrument de toute source d'alimentation électrique avant de retirer les capots. Ne démontez les capots de protection que si cela est explicitement demandé...
  • Page 19 UltiMate 3000 Series: LPG-3400XRS Pump • Ne regardez jamais directement la DEL pour l'éclairage intérieur dans la pompe et ne regardez pas du faisceau lumineux par des instruments qui focalisent le rayon lumineux. L'intensité lumineuse de la lampe peut être nocive pour les yeux. •...

  • Page 20: Intended Use

    UltiMate 3000 Series: LPG-3400XRS Pump 1.3 Intended Use For Research Use Only. Not for use in diagnostic procedures. The device is designed to be operated only be qualified and authorized personnel. All users must know the hazards presented by the device and the used substances. The pump is designed for laboratory research use in high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC) applications.

  • Page 21: Federal Communications Commission (Fcc) Note

    UltiMate 3000 Series: LPG-3400XRS Pump 1.4 Federal Communications Commission (FCC) Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
  • Page 22 UltiMate 3000 Series: LPG-3400XRS Pump Page 14 Operating Instructions...

  • Page 23: Overview

    UltiMate 3000 Series: LPG-3400XRS Pump 2 Overview 2.1 Unit Description The pump is a modern high-quality instrument designed for HPLC analysis, and the heart of the UltiMate 3000 system. The design has been optimized for minimum dead volume. • The quaternary, low-pressure mixing pump provides optimum performance with a maximum operating pressure of 1250 bar.

  • Page 24: Operating Principle

    UltiMate 3000 Series: LPG-3400XRS Pump 2.2 Operating Principle The pump is a quaternary, low-pressure gradient pump with serial dual pistons and electronic compressibility compensation. Thus, the pump forms accurate gradients virtually pulsation-free. The pump head (liquid displacement assembly) includes two cylinders—working cylinder and equilibration cylinder—that are connected in series.

  • Page 25: Functional Schematic

    UltiMate 3000 Series: LPG-3400XRS Pump 2.3 Functional Schematic The picture illustrates how the pump operates. Fig. 1: Operating principle (schematic) Element Inbuilt vacuum degasser Proportioning valve Static mixer (integrated in the proportioning valve) Pump head with Element Working cylinder Equilibration cylinder Purge valve knob Purge valve outlet nozzle Static mixer (integrated in the purge unit)

  • Page 26: Front Panel Elements

    UltiMate 3000 Series: LPG-3400XRS Pump 2.4 Front Panel Elements Fig. 2: Front panel view Element Description Display Shows information about the pump and the status screen (→ page 68) Standby button Switches the pump to Standby mode (the LED is red). To cancel Standby mode and resume operation, press the Standby button again (the LED is not lighted).

  • Page 27: Rear Panel

    UltiMate 3000 Series: LPG-3400XRS Pump 2.5 Rear Panel Fig. 3: Rear panel view Description Power switch (→ page 20) Fuse cartridge (→ page 20) Main power receptacle (→ page 37) Digital I/O port: 8-pin terminal connector for connecting the pump to external devices (→...

  • Page 28: Power Switch

    UltiMate 3000 Series: LPG-3400XRS Pump 2.5.1 Power Switch The power switch on the rear panel is the main power switch for the pump. Turn on the power switch before initial operation of the pump. For routine operation, leave the main power switch on.

  • Page 29: Interior Components

    UltiMate 3000 Series: LPG-3400XRS Pump 2.6 Interior Components Fig. 4: Interior components Description Rear seal wash system (→ page 24) Ports for bayonet-type connectors from the proportioning valve Purge valve knob on the purge unit (→ page 27) Pump head (→ page 25) Containing working cylinder and equilibration cylinder, static mixer, check valves and purge unit Pump lights (here hidden by the front panel door) Leak sensor (→...

  • Page 30: Flow Path

    UltiMate 3000 Series: LPG-3400XRS Pump 2.7 Flow Path The picture illustrates the liquid flow path through the pump. Fig. 5: Flow path through the LPG-3400XRS pump Description Part No. SR-3000 Solvent Rack → page 58 Solvent supply lines to degasser (4 lines) 6043.8109 Analytical 4-channel vacuum degasser ------...

  • Page 31: Fluid Connections

    UltiMate 3000 Series: LPG-3400XRS Pump Description Part No. Bayonet-type connectors from the proportioning valve ------ Pump head containing → section 7.3, page 106 working cylinder and equilibration cylinder (entire assembly), purge unit with purge valve knob and integrated static mixer, displacement chambers with check valve cartridges Rear seal wash tubing from guide bushing to waste Included in 6043.0056...

  • Page 32: Rear Seal Wash System

    UltiMate 3000 Series: LPG-3400XRS Pump 2.9 Rear Seal Wash System The pump is equipped with an active rear seal wash system. Rear seal washing helps avoiding damages to the pistons and piston seals, and thus prolongs the seal lifetime. The rear seal wash system consists of a membrane pump, a wash liquid reservoir, and wash ports on the piston guide bushings to connect the seal wash tubing.

  • Page 33: Pump Head (Liquid Displacement Assembly)

    UltiMate 3000 Series: LPG-3400XRS Pump 2.10 Pump Head (Liquid Displacement Assembly) The pump head contains two displacement chambers, one inlet and one outlet check valve, a purge unit with the pump outlet and a purge valve knob, a static mixer, and two sapphire pistons and their seals.

  • Page 34: Mixing System

    UltiMate 3000 Series: LPG-3400XRS Pump 2.11 Mixing System The pump is equipped with an integrated two-step mixing system including two static mixers. One static mixer is located before the pistons (in the proportioning valve), and the other static mixer is located after the pistons (in the purge unit). The static mixers improve the mixing quality of the combined solvent streams, resulting in a smoother baseline.

  • Page 35: Purge Unit

    UltiMate 3000 Series: LPG-3400XRS Pump 2.12 Purge Unit The purge unit is included in the pump head and comprises a static mixer, the pump outlet, a purge valve knob, and a purge outlet nozzle. Fig. 10: Purge unit in the pump head Description Purge valve knob Purge outlet nozzle...

  • Page 36: Leak Sensor

    UltiMate 3000 Series: LPG-3400XRS Pump 2.14 Leak Sensor A leak sensor is installed inside the pump. If liquid collects in the drip tray under the fluid connections, the leak sensor reports a leak. The Status LED on the front panel door changes to red, a beep sounds and an error is reported in the Chromeleon Audit Trail.

  • Page 37: Operation With Chromeleon

    UltiMate 3000 Series: LPG-3400XRS Pump 2.16 Operation with Chromeleon The pump can be controlled by the Chromeleon Chromatography Management System. To control the pump, an appropriate Chromeleon version and license are required. Two modes of software control are available: Direct Control •...

  • Page 38: Wellness And Predictive Performance

    UltiMate 3000 Series: LPG-3400XRS Pump 2.17 Wellness and Predictive Performance System Wellness in Chromeleon monitors the health of the pump. Therefore, the pump supports several performance and reliability features that can help you detect small problems before they turn into big ones: •...

  • Page 39: Installation

    UltiMate 3000 Series: LPG-3400XRS Pump 3 Installation 3.1 Facility Requirements The installation site must meet the following requirements: • The main power switch and the main power receptacle are on the rear panel. Make sure that ♦ Free and unrestricted access to the main power switch is ensured at all times. ♦...

  • Page 40: Unpacking

    UltiMate 3000 Series: LPG-3400XRS Pump 3.2 Unpacking All electrical and mechanical components of the pump are carefully tested before the module is shipped from the factory. After unpacking, inspect the module for any signs of mechanical damage, which might have occurred during transit. Tips: Immediately report any shipping damage to both, the incoming carrier and Thermo Fisher Scientific.

  • Page 41: Positioning The Pump In The Ultimate 3000 System

    UltiMate 3000 Series: LPG-3400XRS Pump 3.3 Positioning the Pump in the UltiMate 3000 System The positioning of the pump and the arrangement of the system modules depend on the UltiMate 3000 system and the application. Module arrangement with a WPS-3000TXRS autosampler If the pump is part of an UltiMate 3000 system for analytical HPLC applications with a WPS-3000TXRS autosampler, you should stack the individual modules as shown in Fig.
  • Page 42 UltiMate 3000 Series: LPG-3400XRS Pump Solvent Rack Optional Corona or Coulochem III Detector LPG-3400XRS Pump WPS-3000TXRS Autosampler Column Compartment Detector (here DAD-3000/ MWD-3000) Fig. 14: Example for the rear panel connections on an UltiMate 3000 XRS system with WPS-3000TXRS autosampler Apart from the solvent rack, all modules of the UltiMate 3000 system can be connected separately to the Chromeleon data system computer by using the USB port on the rear panel of the module.
  • Page 43 UltiMate 3000 Series: LPG-3400XRS Pump Module arrangement with an OAS-3x00TXRS autosampler If the pump is part of an UltiMate 3000 system for analytical HPLC applications with an OAS-3x00TXRS autosampler, you should stack the individual modules below the autosampler and interconnect them on the rear panel as shown below. OAS-3x00TXRS Autosampler Column Compartment...

  • Page 44: Connecting The Pump

    UltiMate 3000 Series: LPG-3400XRS Pump 3.4 Connecting the Pump 3.4.1 General Information If you want to operate the pump with Chromeleon Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the pump to the USB port on the data system computer and turn on the pump power.

  • Page 45: Connecting The Power Cord

    UltiMate 3000 Series: LPG-3400XRS Pump 3.4.3 Connecting the Power Cord Use the power cord shipped with the device to connect the pump to the main power source. Connect the power cord from the main power receptacle on the rear panel to a grounded power source.
  • Page 46 UltiMate 3000 Series: LPG-3400XRS Pump 3.4.4.1 Connecting a WPS-3000TXRS Autosampler for Stroke Sync If you want to use inject response only In this case, it is not necessary to connect the autosampler directly to the pump. The inject response signal is transmitted via Chromeleon. Thus, only connection to the data system via USB computer is required.
  • Page 47 UltiMate 3000 Series: LPG-3400XRS Pump 3.4.4.2 Connecting an OAS-3x00TXRS Autosampler To connect the OAS-3x00TXRS autosampler to the pump, the following cables are required: Cable Part No. Autosampler interconnect cable —included in the autosampler shipment 6043.0004 For information about the connectors, see page 39. Synchronization cable to connect the autosampler to the pump for pump stroke 6043.0002 synchronization...
  • Page 48 UltiMate 3000 Series: LPG-3400XRS Pump 3. Plug the 8-pin terminal connector of the synchronization cable into the Digital I/O port on the pump. Fig. 19: 8-pin terminal connector on synchronization cable 4. Plug the 15-pin Sub-D connector of the interconnect cable into the Interface 1 port on the autosampler.
  • Page 49 UltiMate 3000 Series: LPG-3400XRS Pump 7. Connect the other end of the RS-232 synchronization cable to the RS-232 port on the Chromeleon data system computer. If an RS-232 port is not available on the data system computer, use the RS-232 to USB Interface cable from the accessories kit for the autosampler for the connection between the RS-232 synchronization cable and the USB port on the computer.
  • Page 50 UltiMate 3000 Series: LPG-3400XRS Pump 3.4.4.3 Connecting a Corona or Coulochem III Detector To connect a Corona or Coulochem III detector to the pump, the following parts are required: Description Part No. Signal synchronization cable from pump to Corona or Coulochem III detector 6043.0003 The cable is included in the accessories kit for the Corona or Coulochem III detector.
  • Page 51 UltiMate 3000 Series: LPG-3400XRS Pump 3.4.4.4 Connecting a WPS-3000TXRS and a Corona or Coulochem III To connect both a WPS-3000TXRS autosampler and a Corona or Coulochem III detector to the Digital I/O port of the pump, the following parts are required: Description Part No.
  • Page 52 UltiMate 3000 Series: LPG-3400XRS Pump 3.4.4.5 Connecting an OAS-3x00TXRS and a Corona or Coulochem III To connect both an OAS-3x00TXRS and a Corona or Coulochem III detector to the Digital I/O of the pump, the following cables are required: Cable Part No.

  • Page 53: Setting Up The Pump In Chromeleon

    UltiMate 3000 Series: LPG-3400XRS Pump 3.5 Setting Up the Pump in Chromeleon This section provides brief instructions for setting up the pump in Chromeleon. For details, see the Chromeleon Help. Tip: When the pump is connected to the data system computer, verify that the Chromeleon software is installed before turning on the pump power for the first time.
  • Page 54 UltiMate 3000 Series: LPG-3400XRS Pump c) Install Chromeleon. d) Turn on the power to the pump. Windows will now detect the pump and install the USB software for the pump automatically. Windows XP will automatically detect the new pump and launch the Found New Hardware Wizard, which guides you through the USB installation.

  • Page 55: Installing The Pump In Chromeleon

    UltiMate 3000 Series: LPG-3400XRS Pump 3.5.2 Installing the Pump in Chromeleon 1. Start the Chromeleon Server Monitor and the Chromeleon server if they are not yet running (→ page 45). 2. Start the Chromeleon Server Configuration program by clicking Start on the taskbar. Point to Programs (or All Programs, depending on the operating system), point to Chromeleon, and then click Server Configuration.

  • Page 56: Configuring The Pump In Chromeleon

    UltiMate 3000 Series: LPG-3400XRS Pump 3.5.3 Configuring the Pump in Chromeleon 3.5.3.1 Initial Installation On each configuration page for the pump, check and change the settings if necessary and select additional settings if needed. You may reopen the configuration pages later again to change the settings (→...
  • Page 57 UltiMate 3000 Series: LPG-3400XRS Pump Devices Page Fig. 21: Devices page Main Device Name • Default name: PumpModule Displays the name used to identify the pump in the installation environment and in the Chromeleon client. To control the pump with the existing control panels, accept the default name.
  • Page 58 UltiMate 3000 Series: LPG-3400XRS Pump Solvents Page Check and change pressure units and enter the names of solvents delivered by the pump if necessary. Fig. 22: Solvents page • Pressure Units The following pressure units are available: bar, psi, MPa. Default setting is "bar". •...
  • Page 59 UltiMate 3000 Series: LPG-3400XRS Pump The signal for the pump pressure (Pump_Pressure) is selected by default. Accept this setting if you want to record the pump pressure. Chromeleon then generates the appropriate channels for data acquisition. For more information, see section 5.4.5 (→ page 82). The duration for the stroke sync signal (500 ms) is entered by default.

  • Page 60: Setting Up The Pump In Dcmslink

    UltiMate 3000 Series: LPG-3400XRS Pump 3.5.3.2 Changing the Configuration Properties To change the standard configuration settings, reopen the configuration pages: 1. Start the Server Configuration program (→ page 47). 2. Right-click the pump in the timebase and click Properties on the menu. 3.

  • Page 61: When A Corona Or Coulochem Iii Detector Is Connected

    UltiMate 3000 Series: LPG-3400XRS Pump 3.7 When a Corona or Coulochem III Detector is connected Note the following if you want to connect a Corona or Coulochem III detector to the pump: • For information about how to connect the detector to the pump, follow the instructions for connecting the Corona or Coulochem III detector on page 42, or follow the instructions in the Chromeleon Help.
  • Page 62 UltiMate 3000 Series: LPG-3400XRS Pump Page 54 Operating Instructions...

  • Page 63: Preparation For Operation (Startup)

    UltiMate 3000 Series: LPG-3400XRS Pump 4 Preparation for Operation (Startup) 4.1 Overview of Actions Important: The pump is filled with 2-propanol when being shipped from the factory. During initial operation of the pump, make sure that the solvents used are miscible with 2-propanol. Otherwise, follow the appropriate intermediate steps.
  • Page 64 UltiMate 3000 Series: LPG-3400XRS Pump 6. Operating the pump with Chromeleon or DCMSLink ♦ Set up the pump in Chromeleon (→ page 45) or DCMSLink (→ page 45) if it is not already set up. ♦ Pump stroke synchronization in Chromeleon If your UltiMate 3000 system includes a WPS-3000TXRS or OAS-3x00TXRS autosampler, you can link the pump with the autosampler for pump stroke synchronization (→...

  • Page 65: General Precautions For Connecting And Handling Capillaries

    UltiMate 3000 Series: LPG-3400XRS Pump 4.2 General Precautions for Connecting and Handling Capillaries When you connect capillaries to the pump, observe the following general precautions: • Observe the precautionary statements for capillaries and capillary connections in section 1.2.2 (→ page 4). •...

  • Page 66: Solvent Reservoirs

    UltiMate 3000 Series: LPG-3400XRS Pump 4.3 Solvent Reservoirs For the secure and functional positioning of the solvent reservoirs, solvent racks and reservoir trays can be used as an ideal complement to the pump. To safely organize your solvent reservoirs, the following options are available in the UltiMate 3000 system: •...

  • Page 67: General Notes

    UltiMate 3000 Series: LPG-3400XRS Pump 4.3.1 General Notes When connecting the solvent reservoirs, observe the following general precautions: Before first use, rinse solvent bottles thoroughly using highly pure solvents. • • Always use the original solvent lines. This prevents contaminants from reaching the HPLC system that may increase wear and cause damage to the system.

  • Page 68: Connecting The Solvent Reservoirs

    UltiMate 3000 Series: LPG-3400XRS Pump 4.3.2 Connecting the Solvent Reservoirs The solvent supply lines from the degasser outlets to the proportioning valve are connected to the pump at the factory. To connect the solvent supply lines from the solvent reservoirs to the degasser inlets, use the solvent supply lines from the accessories kit for the pump and follow the steps below.
  • Page 69 UltiMate 3000 Series: LPG-3400XRS Pump 8. Connect the solvent supply lines from the reservoirs to the inlets of the inbuilt vacuum degasser. Make sure that the supply lines are connected to the degasser inlets correctly. The colors of the ferrules on the degasser inlets should match the colors of the ferrules connected to the degasser outlets.

  • Page 70: Connecting Drain Tubing

    UltiMate 3000 Series: LPG-3400XRS Pump 4.4 Connecting Drain Tubing To discharge liquids that might have accumulated in the interior, the pump has a drain port at the bottom right of the enclosure. Fig. 27: Drain port Direct liquid leaks to waste through the drain system of the UltiMate 3000 system. The required components are shipped with the pump, but can be ordered also separately.
  • Page 71 UltiMate 3000 Series: LPG-3400XRS Pump The tubing from the Out port of the membrane pump to the guide bushings, and the tubing to connect the guide bushings are installed at the factory. To install the remaining tubing and set up rear seal washing, follow the steps below. Description Upper guide bushing Wash port...

  • Page 72: Purging The Pump

    UltiMate 3000 Series: LPG-3400XRS Pump 4.6 Purging the Pump Purging the pump means rinsing the system for a short time at a higher flow rate. The purge cycle has to be initiated manually. During the purge cycle, the pump is purged with the following settings: Purge Flow Purge Time...
  • Page 73 UltiMate 3000 Series: LPG-3400XRS Pump 5. Purge the pump from Chromeleon. a) In Chromeleon, open the Commands dialog box for the pump. b) Set the channel to be purged to 100%. In Chromeleon, you cannot set %A directly. Thus, if %A be 100%, you have to set all other components of the eluent to 0%.

  • Page 74: Equilibrating The System

    UltiMate 3000 Series: LPG-3400XRS Pump 4.7 Equilibrating the System Before using the pump for sample analysis, equilibrate the UltiMate 3000 system: 1. Pump the starting solvent through the entire system until the system is free of any other liquid composition. 2.

  • Page 75: Operation And Maintenance

    UltiMate 3000 Series: LPG-3400XRS Pump 5 Operation and Maintenance The pump is operated with the Chromeleon Chromatography Management System. For details, see section 5.3 (→ page 69). 5.1 Power-Up To start the pump for the first time, turn on the main power switch on the rear panel of the pump.

  • Page 76: Status Screen

    UltiMate 3000 Series: LPG-3400XRS Pump 5.2 Status Screen After the pump has been turned on, the display immediately shows the status screen. LPG-3400XRS 0.500 1138 %A: 100.0 %B: 0.0 %C: 0.0 %D: 0.0 Flow On Fig. 31: Status screen (example) The status screen shows the following information: •...

  • Page 77: Operation With Chromeleon

    UltiMate 3000 Series: LPG-3400XRS Pump 5.3 Operation with Chromeleon Before you begin, verify that 1. The Chromeleon software is installed on the computer and the license code is entered. 2. The pump is connected to the Chromeleon data system computer by means of an USB connection.

  • Page 78: Direct Control

    UltiMate 3000 Series: LPG-3400XRS Pump 5.3.2 Direct Control With direct control, you select operating parameters and commands in the Commands (F8) dialog box. Direct commands are executed as soon as they are entered. For routine operation, most parameters and commands are available also on a control panel. To open the Commands dialog box for the pump 1.
  • Page 79 UltiMate 3000 Series: LPG-3400XRS Pump 6. Verify that the pump is connected to Chromeleon. If it is not, click Connect to connect the pump. For a list of the commands and properties that are supported for the pump, see the Chromeleon Help.

  • Page 80: Automated Control

    UltiMate 3000 Series: LPG-3400XRS Pump 3. Verify that the pump is connected to Chromeleon (the LED next to the Connect button is green). If it is not, click Connect. The control panel provides access to the operating parameters and commands required for routine operation of the pump.
  • Page 81 UltiMate 3000 Series: LPG-3400XRS Pump 2. Change the settings in the program as desired. The easiest way is to edit a program is to do this in the Device Views (→ Fig. 34). Click a device icon and change the settings on the device pages. Editing the program in the Device Views ensures correct command syntax.

  • Page 82: Information For Operating The Pump

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4 Information for Operating the Pump This section provides specific information about settings and functions that should be considered for operating the pump. To learn more about ... See page ... Choosing the solvents Using pump stroke synchronization Setting the flow rate, flow acceleration, and flow deceleration Setting the pressure limits Recording the pump pressure...
  • Page 83 UltiMate 3000 Series: LPG-3400XRS Pump • Mind the special properties of the solvents, such as viscosity, boiling point, UV absorption (UV/VIS detector), refractive index (refractive index detector), and dissolved gas (degasser). • Buffer concentration: Typically up to 1 mol/L (< 0.1 mol/L chloride ions). pH range: 1 through 13 In rare cases, reactions with the stainless steel or titanium have been observed for pH values <...

  • Page 84: Using Pump Stroke Synchronization With The Autosampler

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.2 Using Pump Stroke Synchronization with the Autosampler When an UltiMate 3000 XRS series autosampler is linked to the pump, the inject command of the autosampler can be synchronized with the pump strokes. To set up synchronization, you can specify that the pump is linked to the autosampler in Chromeleon.
  • Page 85 UltiMate 3000 Series: LPG-3400XRS Pump 3. Click the Segments / Pump Link page. Select the pump on the Flow through sampler is delivered by pump(s) list (→ Fig. 35). ♦ If you want to link the autosampler to the pump Select UM3WPS_STRK_1 if the pump is connected to Input_1 (= Digital I/O port 1) on the autosampler.
  • Page 86 UltiMate 3000 Series: LPG-3400XRS Pump 4. Click the Inputs page and select Input_X (where X = the port, to which you connected the pump). If the pump is connected to Digital I/O port 1, for example, select Input_1 (→ Fig. 36). Fig.
  • Page 87 UltiMate 3000 Series: LPG-3400XRS Pump 5. Double-click Input_X and specify the name of the pump under Pump Device (→ Fig. 37). Make sure that you enter the same name as specified on the configuration pages of the pump on the Devices tab under Pump device name (→ page 48). Fig.
  • Page 88 UltiMate 3000 Series: LPG-3400XRS Pump If your UltiMate 3000 system includes an OAS-3x00TXRS autosampler For the proper functioning of the pump stroke synchronization with the OAS-3x00TXRS, settings are required in the Chromeleon Server Configuration and on the control terminal of the autosampler.

  • Page 89: Setting The Flow Rate, Flow Acceleration, And Flow Deceleration

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.3 Setting the Flow Rate, Flow Acceleration, and Flow Deceleration In Chromeleon, you can set how fast the pump starts delivering with the selected flow rate (flow acceleration) and how fast the pump flow is reduced (flow deceleration). •...

  • Page 90: Setting The Pressure Limits

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.4 Setting the Pressure Limits The pump firmware and Chromeleon provide standard values for the upper and lower pressure limits. The limits depend on the pump type. You are free to change the limits within the allowed pressure range.

  • Page 91: Rear Seal Wash System

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.6 Rear Seal Wash System Rear seal washing helps avoiding damages to the pistons, piston seals, and support rings, and thus prolongs the seal lifetime. For information about how to connect the rear seal wash system, see page 62. 5.4.6.1 Working with Rear Seal Washing The rear seal wash system is enabled and cannot be disabled.

  • Page 92: Purging The Pump

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.7 Purging the Pump If you observe pressure pulsation, a high noise level, or pulsation during the operation of the pump or if the analysis is not reproducible, this may indicate that there are air bubbles in the system.

  • Page 93: Vacuum Degasser

    UltiMate 3000 Series: LPG-3400XRS Pump 5.4.10 Vacuum Degasser 5.4.10.1 General Notes for Degasser Operation In normal operation, the degasser is quiet. Even if the vacuum pump is running, the operating noise is very low. With higher load, the operating noise may slightly increase. However, this does not impair the degassing performance.

  • Page 94: Special Chromeleon Functions

    UltiMate 3000 Series: LPG-3400XRS Pump 5.5 Special Chromeleon Functions This section provides a short overview of some special functions that Chromeleon supports for the pump. To learn more about ... See page ... Predictive performance See next section Using the digital inputs Operational Qualification and Performance Qualification All of these functions are available in the Commands dialog box (unless otherwise noted).

  • Page 95: Monitoring The Digital Inputs (Digital I/O)

    UltiMate 3000 Series: LPG-3400XRS Pump Control Panel On the control panel for the pump, click Wellness, Qualification, and Service to see the related predictive performance commands and parameters on separate panels. On these panels, you can enter the limits and reset the counters. In addition, wellness bars provide visual indicators of qualification and service periods.

  • Page 96: Shutting Down The Pump

    UltiMate 3000 Series: LPG-3400XRS Pump 5.6 Shutting Down the Pump Observe the following precautions before interrupting the operation or before shipping the pump: • Rinse out any solvents if necessary. Fill the pump with methanol or a similar alcohol, such as 2-propanol or ethanol. If a buffer is used as a part of the mobile phase, flush the system with several volumes of methanol/water (50:50) before it is shut down.
  • Page 97 UltiMate 3000 Series: LPG-3400XRS Pump Shutdown Program A shutdown program automates shutdown of the HPLC system. The main program steps are: The pump flow is automatically stopped at the end of the program. • • Certain system components and functions are turned off (for example, detector lamps, temperature control).

  • Page 98: Routine And Preventive Maintenance

    UltiMate 3000 Series: LPG-3400XRS Pump 5.7 Routine and Preventive Maintenance The pump is made of high-quality components and materials to minimize maintenance requirements. All surfaces are resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the pump surface, by using lint- free cloth or paper.
  • Page 99 UltiMate 3000 Series: LPG-3400XRS Pump Frequency What you should do... Regularly Check the drain line connected to the drain port at the bottom right of the pump. Verify that the tubing is unclogged and is routed below the drain port. Check the volume of the liquid in the waste container and empty as needed.
  • Page 100 UltiMate 3000 Series: LPG-3400XRS Pump Page 92 Operating Instructions...

  • Page 101: Troubleshooting

    UltiMate 3000 Series: LPG-3400XRS Pump 6 Troubleshooting 6.1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the pump or UltiMate 3000 system. Status LED • The status LEDs (light emitting diodes) on the front panel provide a quick visual check of the operational status of the pump.

  • Page 102: Error Codes On The Pump Display

    UltiMate 3000 Series: LPG-3400XRS Pump 6.2 Error Codes on the Pump Display Each time a fault or error occurs during the operation of the pump, the Status LED on the front panel door changes to red, and an error code appears in the lower left corner on the pump display.
  • Page 103 UltiMate 3000 Series: LPG-3400XRS Pump Error Code Description Remedial Action ("xx") The system pressure has Check whether the flow path after the purge unit is exceeded the set upper blocked. Open the purge valve. pressure limit. If the flow path is blocked - The column may be contaminated.
  • Page 104 UltiMate 3000 Series: LPG-3400XRS Pump Error Code Description Remedial Action ("xx") This code appears after 180 seconds after the leak Leak detected. sensor leak has reported a leak for the pump. The flow is automatically turned off. Find and eliminate the source for the leak. Dry the leak sensor and tray (→...

  • Page 105: Operating Problems

    UltiMate 3000 Series: LPG-3400XRS Pump 6.3 Operating Problems The table provides information about common operating problems that might occur with the pump or an UltiMate 3000 system and lists probable causes, as well as remedial actions. For more information and remedial actions, see the manuals for the other modules of the UltiMate 3000 system.
  • Page 106 UltiMate 3000 Series: LPG-3400XRS Pump Problem Probable Cause Remedial Action The system has very high The high viscosity of the rear seal Check the rear seal wash backpressure. wash solution has a negative solution for viscosity and impact on pressure measuring organic content.
  • Page 107 UltiMate 3000 Series: LPG-3400XRS Pump Problem Probable Cause Remedial Action Pressure pulsation or The solvent is not degassed Degas the eluent. inconstant pressure sufficiently. The solvent is degraded. Use fresh solvent. There are pressure fluctuations Purge the pump (→ page 62). from the pump.
  • Page 108 UltiMate 3000 Series: LPG-3400XRS Pump Problem Probable Cause Remedial Action Additional peaks appear in With gradients, the equilibration Extend the equilibration time. the injection peak. time after the flush cycle is too short. The dead volume is too high. Eliminate possibly existing dead volume.
  • Page 109 UltiMate 3000 Series: LPG-3400XRS Pump Problem Probable Cause Remedial Action Poor peak area precision The sample is unstable and Use new sample or change the (Cont'd) decomposes. conditions. Cool the sample in the autosampler if possible. Baseline fluctuations See the remedial actions provided in the related baseline sections further up in this table.
  • Page 110 UltiMate 3000 Series: LPG-3400XRS Pump Problem Probable Cause Remedial Action Flow fluctuation The inlet path is blocked. Check the inlet lines, filter of the pump, proportioning valve etc. for signs of blockage. There is air in the inlet path. Purge the pump (→ page 62) and check the degasser.

  • Page 111: Service

    UltiMate 3000 Series: LPG-3400XRS Pump 7 Service 7.1 General Notes and Safety Precautions The following sections describe all service and repair procedures for the pump that the user may perform. All other maintenance and service procedures must be performed only by Thermo Fisher Scientific service personnel.
  • Page 112 UltiMate 3000 Series: LPG-3400XRS Pump • The device contains voltage lines. Turn off the main power switch and disconnect the power cord prior to servicing the pump. For instructions on shutting down the pump, see page 88. Tips: Do not forget to test the pump for leakage after you have carried out maintenance or repair work on the fluid connections (→...

  • Page 113: Eliminating Leakage

    UltiMate 3000 Series: LPG-3400XRS Pump 7.2 Eliminating Leakage The leak sensor is installed inside the pump and reports a leak when liquid collects in the drip tray under the fluid connections. Locate the source for the leak, eliminate the cause, and dry the leak sensor.

  • Page 114: Pump Head, Pistons And Piston Seals

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3 Pump Head, Pistons and Piston Seals To replace the piston seals, the check valve cartridges or the purge unit, or to clean the components of the pump head, remove the pump head from the interior front panel, and then disassemble it (→...
  • Page 115 UltiMate 3000 Series: LPG-3400XRS Pump Fig. 41: Components of the pump head Fig. 42: Holding hook with piston and guide bushing Description Part No. Pump head, entire assembly (except proportioning valve), including: 6043.0106 Compression lid ---- Purge unit including 6043.0120 static mixer, purge valve with knob and outlet nozzle Displacement chambers ----...
  • Page 116 UltiMate 3000 Series: LPG-3400XRS Pump Description Part No. Adapter plate for proportioning valve ---- Piston drive unit ---- Piston retaining spring Included in 6043.1954 Holding hook with piston ---- Sapphire piston 6043.0169 Secondary piston seal Included in 6043.1954 Piston guide bushing with ports for rear seal wash tubing ---- containing primary piston seal Holding hook with two clamping screws...

  • Page 117: Visually Inspecting The Pump For Piston Seal Leakage

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.1 Visually Inspecting the Pump for Piston Seal Leakage You can inspect the pump visually for liquid leaks from the piston seals. As the pistons move back and forth within the displacement chambers, a small quantity of mobile phase leaks behind the primary piston seals.

  • Page 118: Pump Head And Pistons

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.2 Pump Head and Pistons Tip: When performing service procedures for the pistons and the piston seals, remove the pistons from the seals as little as possible to preserve the quality of the seals. 7.3.2.1 Removing and Disassembling the Pump Head To remove the pump head from the interior front panel 1.
  • Page 119 UltiMate 3000 Series: LPG-3400XRS Pump 8. Remove the two piston retaining springs on the front by gently sliding them away from the pistons (→ Fig. 44). Make sure that the pistons do not touch the piston drive unit. If they do, move the pistons slightly toward the left.
  • Page 120 UltiMate 3000 Series: LPG-3400XRS Pump To disassemble the pump head Tip: Never disassemble the pump head with bare hands. Put on a pair of clean room gloves to prevent contamination on the pump parts. Even minute particles may cause damage to the system and result in poor pump performance. 1.
  • Page 121 UltiMate 3000 Series: LPG-3400XRS Pump 7.3.2.2 Reassembling and Installing the Pump Head Description Part No. Pump head (entire assembly) 6043.0106 Piston, sapphire 6043.0169 To reassemble the pump head Tip: Make sure you have cleaned all parts of the pump head with isopropanol and a clean wipe before reassembly.
  • Page 122 UltiMate 3000 Series: LPG-3400XRS Pump Fig. 48: Left: Check valve cartridge with orientation sign Right: Displacement chamber with bore for guide bushing (1), and bore for check valve cartridge (2) 2. Hold the main body and slide the complete stack into the main body. 3.
  • Page 123 UltiMate 3000 Series: LPG-3400XRS Pump 6. Carefully pre-align the assembled holding hook with the main body of the pump head by sliding the holding hook with the piston and guide bushing into the main body. Do not push the piston into the seal yet (→ Fig. 50). Fig.
  • Page 124 UltiMate 3000 Series: LPG-3400XRS Pump To install the pump head to the interior front panel 1. Use the guide pins on the interior front panel to carefully place and align the pump head on the interior front panel. Align the pistons so that they do not touch the piston drive unit while installing the pump head.
  • Page 125 UltiMate 3000 Series: LPG-3400XRS Pump 10. Test the pump for leakage (→ page 129). Tighten leaking connections. 11. When you operate the pump under Chromeleon When you replaced the pistons, piston seals or check valve cartridges, update the related service information for predictive performance in Chromeleon. To do so, perform the required command as described in section 5.5.1 (→...

  • Page 126: Replacing The Piston Seals

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.3 Replacing the Piston Seals The piston seals prevent solvent from leaking into the rear seal wash system or into the pump. Leaking may cause unstable flow rates and baseline noise. Each piston has two piston seals: A primary piston seal in the displacement chamber (→...
  • Page 127 UltiMate 3000 Series: LPG-3400XRS Pump 7.3.3.1 Replacing the Primary Piston Seals The primary piston seals are reversed phase (RP) piston seals, and are located in the displacement chambers. Description Part No. Piston seals kit, with 6043.0295 2 primary piston seals 2 secondary piston seals 1.
  • Page 128 UltiMate 3000 Series: LPG-3400XRS Pump 6. Slide the replacement primary seal over the tip of the plunger until it is flush with the surface of the plunger. The open side of the seal must face away from the tool. Fig. 55: Piston seal positioned on the seal handling tool (open seal side facing away) 7.
  • Page 129 UltiMate 3000 Series: LPG-3400XRS Pump 10. Gently remove the seal handling tool from the displacement chamber. 11. Form a stack out of the purge unit, displacement chambers and valve cartridges (→ Fig. 58). The bores for the pistons and piston guide bushings should be on the same side as the pump outlet.
  • Page 130 UltiMate 3000 Series: LPG-3400XRS Pump 4. Gently remove the seal by slightly turning the plunger and applying side force. 5. Carefully move the piston through the bore in the holding hook. 6. Place the holding hook on a clean surface with the hook pointing upward (→ Fig. 60). 7.
  • Page 131 UltiMate 3000 Series: LPG-3400XRS Pump 7.3.3.3 Recommended Actions after Piston Seal Replacement Observe the following recommendations when you have replaced the piston seals in the pump head: Tip: Pump performance and the seal life cycle depend directly on the actions recommended in this section.

  • Page 132: Replacing The Check Valve Cartridge

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.4 Replacing the Check Valve Cartridge Two check valve cartridges are installed in the pump head: one inlet check valve cartridge between proportioning valve and displacement chamber, and one outlet check valve cartridge between the two displacement chambers. The two check valve cartridges and the displacement chambers are identical, respectively.

  • Page 133: Replacing The Purge Unit

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.5 Replacing the Purge Unit The purge unit includes a static mixer, the purge valve knob, the purge valve outlet nozzle and the pump outlet. To replace the purge unit, follow the steps below. Description Part No.

  • Page 134: Replacing The Proportioning Valve

    UltiMate 3000 Series: LPG-3400XRS Pump 7.3.6 Replacing the Proportioning Valve The proportioning valve includes four channels and a static mixer. To replace the proportioning valve, follow the steps below. Description Part No. Proportioning valve, entire assembly 6043.0270 1. Remove the pump head from the interior front panel (→ page 110). 2.

  • Page 135: Cleaning The Pump Head Components

    UltiMate 3000 Series: LPG-3400XRS Pump 7.4 Cleaning the Pump Head Components When performing service procedures to the pump head components, make sure that you clean the components properly with a dry, lint-free tissue and isopropanol to avoid contamination in the system. Be sure that you use clean room gloves. Piston seals should be cleaned in isopropanol for at least 10 minutes before they are re- installed in the pump head.

  • Page 136: Cleaning The Check Valve Cartridges

    UltiMate 3000 Series: LPG-3400XRS Pump 7.4.2 Cleaning the Check Valve Cartridges If you use buffers with high concentrations of salts, clean the cartridges as part of a regularly scheduled, preventive maintenance procedure every 6 to 12 months depending on your usage. Clean the cartridges if they are not functioning properly or if you experience residual pulsation that is too high.

  • Page 137: Testing The Pump For Leakage

    UltiMate 3000 Series: LPG-3400XRS Pump 7.5 Testing the Pump for Leakage After you have carried out any maintenance or repair work on the fluid system, test the pump for leakage. 1. Close the pump outlet with a fitting plug. (Appropriate fitting plugs are provided in the accessories kit of the pump.) 2.
  • Page 138 UltiMate 3000 Series: LPG-3400XRS Pump c) After the stabilization time, monitor the pressure drop. d) Tighten the connection a little more tight. The pressure will suddenly increase a little. e) Monitor if the pressure drops at the same rate as before. If the pressure drops significantly slower, the connection was leaking.

  • Page 139: Vacuum Degasser (Rinsing The Degassing Channels)

    UltiMate 3000 Series: LPG-3400XRS Pump 7.6 Vacuum Degasser (Rinsing the Degassing Channels) To avoid contamination of the degasser, you should prepare fresh solvents, clean the solvent supply lines, and rinse the degassing channels at regular intervals. Rinsing the degassing channels is especially important for the channel that degasses aqueous solvents (for reverse-phase chromatography).

  • Page 140: Replacing The Main Power Fuses

    UltiMate 3000 Series: LPG-3400XRS Pump 7.7 Replacing the Main Power Fuses Warning: Turn off the pump. Disconnect the power cord from its source. Avertissement: Avant de remplacer les fusibles, arrêtez la pompe. Assurez-vous de bien débrancher le cordon d'alimentation de la source secteur. 1.

  • Page 141: Updating The Pump Firmware

    UltiMate 3000 Series: LPG-3400XRS Pump 7.8 Updating the Pump Firmware The pump is shipped with the most recent firmware version. If a newer version of the firmware is available, the Thermo Fluspeed Series Pump Firmware Upgrade Wizard 2.0 is required to update the firmware. Before performing a firmware update, check the firmware version installed in the pump.
  • Page 142 UltiMate 3000 Series: LPG-3400XRS Pump 3. On the Select Pump page, the pump is identified automatically. If two or more pumps are connected to the computer with which you perform the update, select the desired pump. 4. On the Select Action page, select Upgrade firmware. 5.

  • Page 143: Technical Information

    UltiMate 3000 Series: LPG-3400XRS Pump 8 Technical Information Operating Serial dual-piston pump principle Compressibility Fully automated, independent of the composition of the mobile phase compensation Flow rate range Recommended 10 - 2,000 µL/min Settable 1 - 2,000 µL/min Flow rate +/- 0.5 % or 1 µL/min, whichever is greater accuracy Flow rate...
  • Page 144 UltiMate 3000 Series: LPG-3400XRS Pump Wetted parts Stainless steel, titanium, sapphire, aluminum oxide (Al2O3), PEEK™, PTFE, FEP, UHMW polyethylene, perfluoro-elastomer, nickel/molybdenum alloy, ruby Power 100-115V, 60 Hz; 200-240V, 50 Hz; max. 220 VA; 1,9A @115V, 1,0A@230V requirements Emission sound < 70 dB(A) in 1-m-distance pressure level: Environmental Range of use: Indoor use;...

  • Page 145: Accessories, Consumables, And Spare Parts

    UltiMate 3000 Series: LPG-3400XRS Pump 9 Accessories, Consumables, and Spare Parts Accessories, spare parts, and consumables for the pump are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. 9.1 Standard Accessories Description Part No.
  • Page 146 UltiMate 3000 Series: LPG-3400XRS Pump Description Part No. Quantity in the kit Components for UltiMate 3000 system drainage: Included in 6040.0005 Cable clips (self-adhesive) Y piece T piece Elbow Connecting tube Drain tubing Installation instructions HPLC Troubleshooting Guide Poster 6040.0050 Plastic syringe (12 mL) Included in 6000.0010 1 syringe...

  • Page 147: Optional Accessories

    UltiMate 3000 Series: LPG-3400XRS Pump 9.2 Optional Accessories Description Part No. Capillaries, kit of capillaries, for UltiMate 3000 systems with 6845.2301A standalone OAS-3x00TXRS The kit includes the following Viper capillaries for connecting UltiMate 3000 modules in a system with a standalone OAS-3x00TXRS: 4 Viper capillaries, stainless steel (1 each of 0.10 x 65 mm, 0.10 x 250 mm, 0.13 x 750 mm, and 0.18 x 750 mm (I.D.

  • Page 148: Consumables And Spare Parts

    UltiMate 3000 Series: LPG-3400XRS Pump 9.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products. Description Part No.
  • Page 149 UltiMate 3000 Series: LPG-3400XRS Pump Description Part No. Piston seals set, reversed phase 6043.0295 2 primary piston seals 2 secondary piston seals Piston, sapphire, 2 pistons 6043.0169 Power cord, Australia, China 6000.1060 Power cord, Denmark 6000.1070 Power cord, EU 6000.1000 Power cord, India/SA 6000.1090 Power cord, Italy...
  • Page 150 UltiMate 3000 Series: LPG-3400XRS Pump Page 142 Operating Instructions...

  • Page 151: Reference Information

    UltiMate 3000 Series: LPG-3400XRS Pump 10 Reference Information 10.1 Chemical Resistance of PEEK PEEK has superb chemical resistance to most organic solvents. However, it tends to swell when in contact with trichlormethane (CHCl ), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF). In addition, it is attacked by concentrated acids, such as, sulfuric acid and nitric acid or a mixture of hexane, ethyl acetate, and methanol.
  • Page 152 UltiMate 3000 Series: LPG-3400XRS Pump Medium Concentration Temperature Maximum Resistance Duration (+ = yes; - = no) (Days) Citric acid Copper sulphate Cyclohexane Cyclohexanol Cyclohexanone Diethyl ether Diisopropyl ether Dimethylformamide Dioctylphthalate Dioxan Ethanol 96 (Vol.) Ethyl acetate Ethylene glycol Ferric chloride Formaldehyde Formic acid Glycerin...
  • Page 153 UltiMate 3000 Series: LPG-3400XRS Pump Medium Concentration Temperature Maximum Resistance Duration (+ = yes; - = no) (Days) Phenol Concentrated Potassium dichromate Potassium hydroxide Potassium nitrate Potassium permanganate Propane Propyl alcohol Pure benzene Silicone oil Sodium chloride Sodium hydrogen carbonate Sodium hydroxide Sodium hydroxide Sodium thiosulphate...

  • Page 154: Solvent Miscibility

    UltiMate 3000 Series: LPG-3400XRS Pump 10.2 Solvent Miscibility Miscibility describes the ability of liquids to form homogeneous mixtures in all proportions (one-phase system). Solvent miscibility is important during elution and when changing from one solvent to another. Thus, when you prepare solvents consider the miscibility and homogeneous mixing of the single components.

  • Page 155: Properties Of Common Solvents

    UltiMate 3000 Series: LPG-3400XRS Pump 10.3 Properties of Common Solvents The table summarizes the properties of the most important solvents in HPLC [1, 2] UV Transmission at [nm] 20% (0.7 AU) 80% (0.1 AU) 98% (0.01 AU) < 190 Refraction Index (RI) at 20 °C 1.344 1.424 1.376...

  • Page 156: Safety Information About Flammable Solvents

    UltiMate 3000 Series: LPG-3400XRS Pump 10.4 Safety Information about Flammable Solvents The following table provides an overview of safety information for flammable solvents in HPLC Boiling point (°C) Vapor pressure (hPa) Flash point (°C) Auto-ignition temperature (°C) 3-16 2-36 3-19 2-36 2-12 7-36...
  • Page 157 UltiMate 3000 Series: LPG-3400XRS Pump References [1] Otto-Albrecht Neumüller, Römpps Chemie-Lexikon, 8. Auflage, 1987 [2] W.E. Baker et al., Explosion Hazards and Evaluation, Elsevier Sci. Publ., 1983 [3] H. Bennett, Concise Chemical and Technical Dictionary, Edward Arnold Ed., 1986 [4] D.R. Lide, Handbook of Chemistry and Physics, 79 Edition, CRC Press, 1998-1999 [5] G.W.C.
  • Page 158 UltiMate 3000 Series: LPG-3400XRS Pump Page 150 Operating Instructions...

  • Page 159: Appendix ─ Digital I/O (Pin Assignment)

    UltiMate 3000 Series: LPG-3400XRS Pump 11 Appendix ─ Digital I/O (Pin Assignment) The Digital I/O port of the pump is an 8-pin I/O terminal connector, which provides two TTL inputs and three digital outputs that can be used to exchange digital signals with external devices.
  • Page 160 UltiMate 3000 Series: LPG-3400XRS Pump Pin Assignment: WPS-3000TXRS Autosampler to the Pump To connect the pump to a WPS-3000TXRS autosampler, use the signal cable (part no. 6000.1004) and connect it to the separate 8-pin I/O terminal connector from the accessories kit of the pump. Insert the wires of the signal cable into the pins of the terminal connector as shown in the table below.
  • Page 161 UltiMate 3000 Series: LPG-3400XRS Pump Connecting an OAS-3x00TXRS autosampler in addition To connect an OAS-3x00TXRS autosampler in addition to the detector to the pump, connect the signal synchronization cable (part no. 6043.0003) to the 8-pin I/O terminal connector on the synchronization cable (part no. 6043.0002) as shown in the table below. Make sure that the wires of the synchronization cable stay in the correct pins while inserting the wires of the signal synchronization cable from the detector.
  • Page 162 UltiMate 3000 Series: LPG-3400XRS Pump Page 154 Operating Instructions...

  • Page 163: Index

    UltiMate 3000 Series: LPG-3400XRS Pump 12 Index WPS-3000TXRS Autosampler ..... 38 WPS-3000TXRS with Corona ...... 43 WPS-3000TXRS with Coulochem III ..43 Accessories Consumables ........... 140 Optional ............139 Control Standard ............137 Automated ............ 72 Chromeleon ..........69 Direct ............70 Batch ..............
  • Page 164 UltiMate 3000 Series: LPG-3400XRS Pump Replacement ..........118 Run-in ............123 Injection (Stroke Synchronization) Secondary Piston Seal Replacement ... 121 OAS-3x00TXRS ........... 80 Solvent Compatibility ........75 WPS-3000TXRS ........... 76 Testing for Leakage ........109 Injection (Synchronization) ....... 76 Pistons Inputs (Dialog Box) ...........
  • Page 165 UltiMate 3000 Series: LPG-3400XRS Pump Connect ............62 Startup ............... 55 Wash Liquid ..........83 Actions ............55 Server Configuration Program ......52 Connecting Capillaries ........57 Service System Equilibration ........66 Check Valve Cartridges ......124 Static Mixer ............26 Cleaning Check Valve Cartridges ....
  • Page 166 UltiMate 3000 Series: LPG-3400XRS Pump Page 158 Operating Instructions...

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