Overview; Unit Description - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

2 Overview

2.1 Unit Description

The NCS-3500RS and NCP-3200RS are the "heart" of the UltiMate 3000 RSLCnano
System. They perform equally well as flexible and reliable modules for routine analysis
and sophisticated research tasks in nano LC and capillary LC, and can be used in numerous
laboratory environments.
• The NCS-3500RS combines a binary high-pressure gradient pump (NC pump), and a
ternary low-pressure micro pump as loading pump, and a column compartment in one
enclosure.
The NCP-3200RS is a binary high-pressure gradient pump (NC pump). This pump is
intended for use in application setups that do not require a loading pump and column
compartment. Thus, the NCP-3200RS is the ideal choice to support the NCS-3500RS as
a second pump, for example, in parallel LC applications.
• The module has been especially designed to work at higher working pressures (up to
80 MPa with Classic flow meter and up to 90 MPa with ProFlow™ flow meter) and thus,
is the appropriate choice for ultra-high performance LC (UHPLC) applications.
• Key features of the pump module
♦ The NC pump includes two highly precise constant pressure pumps that are
controlled by closed control loops and that enable continuous operation without the
need of refill cycles.
♦ The flow meter controls the partial flows of the two channels.
♦ The integrated control principle ensures that the NC pump delivers highly precise
gradients with the low flow rates required for nano, capillary and micro LC
applications. The flow rate is kept stable, independent of backpressure or
temperature changes.
♦ Eluent mixing is performed at the outlet of the flow meter, thus ensuring that the
gradient delay volume is as small as 25 nL.
♦ Continuous direct flow delivery ("splitless") of the NC pump reduces the solvent
consumption considerably compared to splitter-based systems.
♦ The NC pump and the loading pump are fitted with floating pistons, allowing
compensation for small mechanical tolerances within the specification and thus
enhancing the robustness of the pump.
♦ For the loading pump, the patented isokinetic pre-compression allows a precise and
almost pulse-free flow.
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 17