Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual
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Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

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Thermo Scientific Dionex
UltiMate 3000 Series
Automated Fraction Collector
AFC-3000
Operating Instructions
(Original Operating Instructions)
Revision: 1.2
Date: October 2013
© 2013 Thermo Fisher Scientific
Doc No. 4820.7250

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  • Page 1 Thermo Scientific Dionex UltiMate 3000 Series Automated Fraction Collector AFC-3000 Operating Instructions (Original Operating Instructions) Revision: 1.2 Date: October 2013 © 2013 Thermo Fisher Scientific Doc No. 4820.7250...
  • Page 2 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Operating Instructions...
  • Page 3 AFC-3000 Automated Fraction Collector Declaration of Conformity ( O r i g i n a l D e c l a r a t i o n ) Product: Thermo Scientific Dionex UltiMate 3000 - Fraction Collector Type: AFC-3000 Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: •...
  • Page 4 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page II Operating Instructions...

  • Page 5: Table Of Contents

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Table of Contents Introduction ........................1 1.1 How to Use This Manual ................... 1 1.2 Safety Information ..................... 2 1.2.1 Symbols on the Fraction Collector and in the Manual ........2 1.2.2 Safety Precautions ....................3 1.2.3 Consignes de Sécurité...
  • Page 6 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 4.3 Testing the Interface ....................43 4.4 Testing the Drop Former Alignment ................ 44 Operation and Maintenance ..................45 5.1 Power-Up ......................... 45 5.2 Operation with Chromeleon ..................45 5.2.1 Connecting to Chromeleon ................46 5.2.2 Direct Control ....................

  • Page 7: Introduction

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 1 Introduction 1.1 How to Use This Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader. However, in order to obtain a full understanding of the fraction collector, Thermo Fisher Scientific recommends that you review the manual thoroughly before operating the fraction collector.

  • Page 8: Safety Information

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 1.2 Safety Information The CE Mark label on the instrument indicates that the instrument is compliant with the related standards (→ page I). 1.2.1 Symbols on the Fraction Collector and in the Manual The table below shows the symbols used on the fraction collector: Symbol Description...

  • Page 9: Safety Precautions

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector At various points throughout the manual, messages of particular importance are indicated by certain symbols: Tip: Indicates general information, as well as information intended to optimize the performance of the instrument. Important: Indicates that failure to take note of the accompanying information could cause wrong results or may result in damage to the instrument.
  • Page 10 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • Hazardous substances Many organic solvents, mobile phases and samples are harmful to health. Be sure that you know the toxic and infectious properties of all substances that you are using. You may not know the toxic or infectious properties of many substances that you are using. If you have any doubt about a substance, treat it as if it contains a potentially harmful substance.
  • Page 11 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • Capillaries, capillary connections, open connections ♦ Capillaries, especially non-metallic capillaries may burst, slip out of their fittings or may not be screwed in. This may result in substances spraying out of the open connections.

  • Page 12: Consignes De Sécurité

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • If a leak occurs, turn off the fraction collector immediately, stop the pump flow, and remedy the situation. • Use only standard solvents (HPLC grade) and buffers that are compatible with all parts that may be exposed to solvents.
  • Page 13 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • Substances dangereuses De nombreux solvants organiques, phases mobiles et échantillons sont nuisibles à la santé. Informez-vous de propriétés toxicologiques et infectieuses de toutes les substances que vous utilisez. Les propriétés toxicologiques et infectieuses de nombreuses substances peuvent être mal connues.
  • Page 14 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Notez que un faiseur des gouttes isolée et mise à la terre est disponible pour applications en phase normale (référence 6702.0400). Utilisez les solvants de type phase normale uniquement avec le collecteur de fraction AFC-3000 modifié à l’aide du cette faiseur des gouttes isolée et mise à...
  • Page 15 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • Réglez toujours une limite de pression minimum pour la pompe HPLC. Ceci prévient les dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe. • Le collecteur de fraction pèse plus de 8 kg (17.7 livres). Par conséquent, soulevez le collecteur de fraction avec précaution.

  • Page 16: Intended Use

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 1.3 Intended Use For Research Use Only. Not for use in diagnostic procedures. The device is designed to be operated only be qualified and authorized personnel. All users must know the hazards presented by the device and the used substances. The fraction collector is designed for laboratory research use in high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC) applications.

  • Page 17: Federal Communications Commission (Fcc) Note

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Avertissement: Si l'instrument est utilisé de façon non spécifiée par Thermo Fisher Scientific, la protection prévue par l'instrument pourrait être altérée. Thermo Fisher Scientific n'assume aucune responsabilité et ne sera pas responsable des blessures de l'operateur et/ou des dommages de l'instrument.
  • Page 18 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 12 Operating Instructions...

  • Page 19: Overview

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2 Overview 2.1 Unit Description The fraction collector can be operated with the UltiMate 3000 or any other (U)HPLC system. The design has been optimized for robust and easy use as well as for minimized carry-over volume.

  • Page 20: Operating Principle

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.2 Operating Principle The AFC-3000 Automated Fraction Collector collects fractions from the UltiMate 3000 or any other HPLC system. A solenoid diverter valve directs the flow either to the waste or to the fraction collection tubes. When Chromeleon sends a trigger signal to the AFC-3000 calling for fractions to be collected, the diverter valve moves over the assigned collection tube and is then switched to route eluent flow into the tube.

  • Page 21: Fraction Collector Configurations

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.3 Fraction Collector Configurations The fraction collector is available in the following standard configuration for flow rates up to 150 mL/min: Description Part No. AFC-3000 Automated Fraction Collector, including: 5702.1000 - two 90 position racks - 180 x 8 mL tubes, 13 mm OD - 1.6 mm ID diverter valve and 1 mm ID PEEK drop former Kit for low flow rates...
  • Page 22 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Avertissement: N'effectuez pas d'applications en phase normale sans vous être assuré que votre faiseur des gouttes est proprement isolée et mise à la terre. Les solvants de la phase normale, non-conducteurs et non-polaires, par exemple hexane, peuvent entraîner une accumulation de charges électrostatiques sur le faiseur des gouttes en l’absence de mise à...

  • Page 23: Front Panel Elements

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.4 Front Panel Elements Fig. 1: Front panel view Front Panel Element Description Power indicator LED The LED is illuminated blue when the fraction collector is turned on. Fraction collector arm Transports the diverter valve assembly fore-and-aft (→ see also section 2.2, page 14).

  • Page 24: Rear Panel

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.5 Rear Panel Fig. 2: Rear panel view Description Type label Auxiliary I/O connection Connects to diverter valve assembly to provide power to the diverter valve and grounding to the stainless steel drop former (if equipped). Power switch Main power receptacle Serial communication port (unused)

  • Page 25: Power Switch

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.5.1 Power Switch The power switch on the rear panel is the main power switch for the fraction collector. Turn on the power switch before initial operation of the fraction collector. Turn off the main power switch when instructed to do so, for example, before performing a service procedure or when interrupting operation for longer time periods.

  • Page 26: Universal Fraction Tray, Racks And Tubes

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.6 Universal Fraction Tray, Racks and Tubes The fraction collector supports a large variety of fraction collection racks, including most of the Isco Foxy Jr./R1 and wellplate sampler racks. For information about how to install a rack, see page 30.

  • Page 27: Chromeleon Software

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2.7 Chromeleon Software The fraction collector can exclusively be controlled by the Chromeleon Chromatography Management System. For operating the fraction collector with Chromeleon, the following licenses are required: • Timebase Class 1 • Fraction Collection (part no. 5960.0038) for basic fraction collection, with wizard-based program setup and fraction reporting.
  • Page 28 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 22 Operating Instructions...

  • Page 29: Installation

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3 Installation 3.1 Facility Requirements The AFC-3000 operates reliably even under less than ideal conditions. It is not, however, indestructible. Malfunction or damage can occur if specific operating conditions are not met. Meeting these conditions requires that you create a proper lab environment, replace fraction collector components that wear out under normal use, and purchase the appropriate supplies for use with the fraction collector.

  • Page 30: Unpacking

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • Observe the same general electrostatic discharge precautions as with any other integrated circuit electronic devices. Low-humidity environments, especially when combined with static-generating materials, require maximum care. Important: Discharge static buildup and ground to the fraction collector base or cabinet before performing any maintenance.
  • Page 31 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 1. Place the shipping container on the floor and remove any accessories. 2. Grasp the fraction collector by the sides. Slowly and carefully, pull the instrument out of the shipping container and place it on a stable surface. Important: The fraction collector weighs more than 8 kg (17.7 lbs).

  • Page 32: Installing The Fraction Collector

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.3 Installing the Fraction Collector The AFC-3000 is designed for easy installation consisting of two parts; assembling the fraction collector and connecting it to the analytical instrument. For the most part, you can install the AFC-3000 without using tools. In fact, using tools such as screwdrivers or pliers to perform most installation tasks may result in a damaged or unusable instrument.

  • Page 33: Mounting The Diverter Valve Assembly

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.3.1 Mounting the Diverter Valve Assembly Mounting the diverter valve assembly on the fraction collector arm is the first major task. The diverter valve assembly attaches to the fraction collector arm using two knurled finger-tight screws.

  • Page 34: Diverter Valve Connections

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.3.2 Diverter Valve Connections When connecting capillaries to the fraction collector, observe the following general precautions: • Observe the precautionary statements for capillaries and capillary connections in section 1.2.2 (→ page 3). • When you connect capillaries, make sure that the connectors are free from contaminants.

  • Page 35: Attaching The Rinse Station

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.3.3 Attaching the Rinse Station The rinse station is located at the extreme left position of the fraction collector head (→ Fig. 1, page 17). This station is used to dispense liquid to waste through normal operation of the HPLC system operation.

  • Page 36: Placing Fraction Racks On The Universal Drip Tray

    (Fig.9). Other rack types such as Thermo Scientific Dionex well-plate sampler racks and the 4 x 96 well plates require special adapters which can be ordered separately. The adapter block is correctly installed on the drip tray in one position only, when the recess on the bottom of the adapter block is properly aligned with the elevated parts of the drip tray.
  • Page 37 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Important: Before loading or unloading any fraction racks on the universal drip tray, move the arm with the drop former to the home position by cycling the power off and on (or by sending the RinsePosition comand).
  • Page 38 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fraction rack Maximum number of racks/bottles Rack layout 96Pos Four well plates (96 positions) can be installed on the well plate adapter. Well plates must not be mixed with other rack types. 10Pos_WPS, Four WPS-3000 well-plate sampler racks can 22Pos_WPS, be installed on the rack adapter.

  • Page 39: Adjusting The Drop Former Height

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Tip: To prevent the universal drip tray from accidentally being lifted out of its proper position, always remove the rack adapter toward the side as shown here: Fig. 11: Removing the rack adapter 3.3.6 Adjusting the Drop Former Height 1.
  • Page 40 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Adjustment locking lever Diverter valve Drop former Fig. 12: Diverter valve assembly with drop former 4. Slide the rinse station up or down in its holder so that the lowest part of the drop former is slightly above the top of the rinse station when it is in the rinse position.

  • Page 41: Connecting The Fraction Collector

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.4 Connecting the Fraction Collector The next steps in the installation process involve connecting the AFC-3000 to the power source and to the Chromeleon computer. The following sections explain how to establish these connections. 3.4.1 Connecting the External Desktop Power Supply A voltage-specific external desktop power supply is delivered with each AFC-3000.

  • Page 42: General Communications Information

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.4.2 General Communications Information To connect the fraction collector to the Chromeleon computer, select one of the following alternatives: • Use an RS-232 cable (part no. 8914.0153, shipped with the instrument) to connect the COM 1 port on the rear panel of the fraction collector to a serial COM port on the computer (→...
  • Page 43 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector • The folder contains the 2.08.24 folder (or a higher number) and further sub folders and PDF files: The installation instructions (PDF file) for the drivers can be found in this folder. Install the drivers as described in the instructions for your operating system.
  • Page 44 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fig. 13: Rear panel connections on an UltiMate 3000 system with AFC-3000 (Example) Page 38 Operating Instructions...

  • Page 45: Setting Up The Fraction Collector In Chromeleon

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 3.5 Setting Up the Fraction Collector in Chromeleon This section provides brief instructions for installing and configuring the fraction collector in Chromeleon. Tip: The minimum requirement for control of the AFC-3000 is Chromeleon 6.80 SR7e (Driver Update).

  • Page 46: Configuring The Fraction Collector

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 11. Open the Add device to timebase dialog box again. On the Manufacturers list, click Generic and on the Devices list, click Fraction Collection. Accept the settings on the two configuration pages, then click OK. 12.
  • Page 47 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector AFC Page This page is used to configure the connection between Chromeleon and the fraction collector. Fig. 15: AFC page • Device Name The name used to identify the fraction collector in the installation environment and in the Chromeleon client program.
  • Page 48 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 42 Operating Instructions...

  • Page 49: Verifying Installation

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 4 Verifying Installation 4.1 Overview of Actions Once the installation of the AFC-3000 has been completed, it is important to verify the correct installation to ensure proper functionality of the fraction collector. Installation verification of the fraction collector consists of three parts: •...

  • Page 50: Testing The Drop Former Alignment

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5. Connect the control panel to the timebase in which the fraction collector is installed. On the Control menu, select Connect to Timebase, and then select the timebase on the Timebase tab. For information about the Timebase dialog, click Help. 6.

  • Page 51: Operation And Maintenance

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5 Operation and Maintenance The AFC-3000 is both reliable and easy to use. Before using it, however, ensure that your lab environment provides operating conditions that will prolong the life of the AFC-3000. When you finish using the fraction collector, you may need to flush the rinse station and flow path before shutting the fraction collector down.

  • Page 52: Connecting To Chromeleon

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.2.1 Connecting to Chromeleon 1. Start the Chromeleon Server Monitor if it is not already running (→ page 39). 2. Click Start to start the server. 3. Start the Chromeleon client by clicking the Chromeleon icon on the desktop.
  • Page 53 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5. Change the display filter level if necessary. Right-click in the commands list and select the filter level on the menu. Fig. 16: Commands dialog box 6. Verify that the fraction collector is connected to Chromeleon. If it is not connected, execute the Connect command.
  • Page 54 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fig. 17: AFC-3000 Control Panel The control panel provides access to the operating parameters and commands required for routine operation of the fraction collector. Additional functions are available in the Commands dialog box. To open the Commands box from the panel tabset, select Command on the Control menu.

  • Page 55: Setting The Rack Configuration

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.2.3 Setting the Rack Configuration Define the installed rack types in Chromeleon. It is not recommended to define a rack type in a program file. Important: The rack configuration must not be changed during fraction collection, or during the course of a program file or sequence.

  • Page 56: Defining The Collect Mode

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.2.4 Defining the Collect Mode Define how the drop former travels across the racks. Important: The CollectMode must not be changed during fraction collection, or during the course of a program file or sequence. Important: Ne changez pas le CollectMode au cours de la collection de fraction, ou au cours d'un programme ou séquence.

  • Page 57: Defining The Movement Mode

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.2.5 Defining the Movement Mode Define the diverter valve behavior during any drop former movements: 1. Open the Commands dialog box (→ page 46). 2. Click the plus sign beside AFC. 3. Click MovementMode. In the MovementMode box, select the movement mode: Interrupt (default): If the diverter valve is open ("Collect") prior to any movement of the drop former, the diverter valve will always close ("Drain") prior to a movement and will open again ("Collect") after the target position has been reached.

  • Page 58: Automated Fraction Collection With Chromeleon

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.2.6 Automated Fraction Collection with Chromeleon The Fraction Collection driver in Chromeleon facilitates the process of collecting fractions. Before you start, verify that this driver is installed in the same timebase as the AFC-3000 (→...
  • Page 59 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fraction Collection - Collection Device Options Enter collection parameters, such as the maximum tube volume, maximum number of tubes per fraction, or total number of tubes. • Type the volume of the installed tubes in the Max. tube volume box. •...
  • Page 60 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fraction Collection - Peak Detection Options Modify the peak detection algorithm for fraction collection if required (→ Fig. 22). For more information, see the Chromeleon Help. Fig. 22: Fraction Collection Wizard: Peak Detection Options page Page 54 Operating Instructions...
  • Page 61 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Fraction Collection - Delay and Detector Offset Options Set the delay time or delay volume between the first detector in the system and the fraction collector. • Delay Time: This is a constant value which should only be applied with a constant pump flow rate of your method.
  • Page 62 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector To modify a program 1. Open an existing program. Select and double-click the program you want to open. —or— On the File menu, select Open. In the dialog box, select Program on the Object of Type list and select the program.

  • Page 63: Shutting Down The Fraction Collector

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector To start a program Program for sample analysis 1. Create a sample list (sequence). A sequence must include the program and a method for evaluating the sample data (for example, for peak identification, area determination, and amount determination).

  • Page 64: Routine And Preventive Maintenance

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5.5 Routine and Preventive Maintenance Routine maintenance of the AFC-3000 consists of regular cleaning of specific fraction collector components. Routine maintenance also includes checking AFC-3000 components for leaks or other damage. Additional periodic maintenance tasks may be required, including replacement of the following fraction collector components: sample inlet tubing, rinse station tubing, and fraction tray.

  • Page 65: Troubleshooting

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 6 Troubleshooting 6.1 General Notes The AFC-3000 is both easy to operate and reliable. When the fraction collector does not function properly, isolate the problem to determine if it originates in the Chromeleon computer, the analytical instrument, the communications cable, or the fraction collector itself.

  • Page 66: Messages In The Chromeleon Audit Trail

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Problem Probable Cause Remedial Action The AFC-3000 does The fraction collector is not Check the configuration for the correct not work correctly correctly configured in COM port selection when controlled by Chromeleon. (→ section 3.5.2; page 40). Chromeleon.

  • Page 67: Service

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 7 Service This chapter explains how to clean the AFC-3000, inspect it for leaks, and replace damaged components. Important: Discharge static buildup and ground to the fraction collector base or cabinet before performing any maintenance. Do not touch or short-circuit bare contacts (for example, COM1 or auxiliary ports).

  • Page 68: Cleaning The Fraction Collector

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Before starting maintenance or service procedures, observe the following precautions: • For all service and repair procedures, observe all precautionary statements provided in these operating instructions. • When performing maintenance or repair work on the fluidic components, always move the diverter valve assembly into the appropriate position to facilitate safe working conditions and to allow access to all components without obstructions.

  • Page 69: Weekly Cleaning

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 2. Wipe the fraction tray, fraction collector cabinet, and fraction collector arm using a towel dampened with a lab-grade cleaning agent. Important: Do not allow the cleaning agent to come into contact with the lead screws.

  • Page 70: Installing The Kit For Normal-Phase Lc

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 7.4 Installing the Kit for Normal-Phase LC Description Part No. Kit for normal-phase LC, including: 6702.0400 1.0 mm ID stainless steel drop former and grounding cable Warning: Do not perform normal-phase applications without a proper grounding protection of the drop former.
  • Page 71 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5. Remove the ground cable screw and reinstall with the normal-phase grounding cable attached. Make sure to lead cable along the front side of the diverter valve assembly (→ Fig. 26). Ground cable screw Fig.

  • Page 72: Installing The Kit For Low Flow Rates

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 7.5 Installing the Kit for Low Flow Rates Description Part No. Kit for low flow rates, including: 6702.0300 1.0 mm ID diverter valve with 0.4 mm ID PEEK drop former The kit for low flow rates includes a tool for installation and a piece of 0.3 mm ID PTFE tubing.

  • Page 73: Replacing The Diverter Valve

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 9. Install the diverter valve connections (→ section 3.3.2, page 28). A low-flow inlet capillary is shipped with the kit. 10. Verify that the alignment is still correct (→ page 44). Tips: After installing the low-flow inlet capillary, always determine the delay time or volume.
  • Page 74 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 5. Loosen the two finger-tight knurled screws that attach the rear cable and grounding cable to the diverter valve assembly (→ Fig. 29). Rear cable screw Ground cable screw Fig. 29: Cable screws on the diverter valve assembly 6.

  • Page 75: Replacing The Universal Drip Tray

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 7.7 Replacing the Universal Drip Tray For proper operation, the universal drip tray must be properly seated on the base plate of the fraction collector. An improperly installed universal drip tray may lead to the impression that the fraction collector is misaligned.
  • Page 76 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 70 Operating Instructions...

  • Page 77: Technical Information

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 8 Technical Information Tube capacity: Depends on rack configuration; standard configuration: 180 x 8 mL tubes (13 mm x 100 mm) Tube change time: Typically 0.2 to 0.4 s, depending on collect mode and rack type Maximum tube height: 150 mm Maximum flow rate:...
  • Page 78 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 72 Operating Instructions...

  • Page 79: Accessories, Spare Parts, And Consumables

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 9 Accessories, Spare Parts, and Consumables Accessories, spare parts, and consumables for the fraction collector are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. Because the life-span of the fraction tubes varies, you should maintain an adequate supply of spare tubes.

  • Page 80: Optional Accessories

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 9.2 Optional Accessories Optional Accessory Part No. Remarks Kit for low flow rates 6702.0300 Kit for flow rates up to 10 mL/min. Includes different diverter valve assembly, drop former, and tubing. Kit for normal-phase LC 6702.0400 SST drop former for use with normal- phase solvents.

  • Page 81: Consumables And Spare Parts

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 9.3 Consumables and Spare Parts The part number always refers to the packing unit. Unless otherwise stated, the packing unit is 1 unit. For more information, contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products.
  • Page 82 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Page 76 Operating Instructions...

  • Page 83: Appendix - Chromeleon Example Program

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 10 Appendix - Chromeleon Example Program ;Example program for fraction collection with the AFC-3000 Automated Fraction Collector and the following instruments: HPG-3x00, WPS-3000SL, VWD-3x00 TempCtrl = Temperature.Nominal = 20.0 [°C] Temperature.LowerLimit = 4.0 [°C] Temperature.UpperLimit = 45.0 [°C] ReadyTempDelta =...
  • Page 84 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector ;the current position of the fraction collection driver prior to a run ;***************************************************************** ;* Definition of triggers for fraction collection starts here. ;***************************************************************** Trigger FracStart FracStartDetected AFC.Valve = Collect AFC.TubePosition FractionCollection.TubePosition EndTrigger Trigger TubeChange FracTubeChange AFC.Valve =...
  • Page 85 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector PeakStartThreshold = 10.00 [signal] PeakStartSlope = 0.500 [signal/s] PeakStartTrueTime = 1.00 [s] PeakEndThreshold = 9.00 [signal] PeakEndSlope = -1.000 [signal/s] PeakEndTrueTime = 1.00 [s] DerivStep = 1.00 [s] ThresholdNoPeakEnd = 2000.00 [signal] ThresholdDoNotResolve = PeakMaxSlope = PeakMaxTrueTime = 1.00 [s]...
  • Page 86 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector ;***************************************************************** ;* Sample run ends here. ;***************************************************************** Page 80 Operating Instructions...

  • Page 87: Index

    UltiMate 3000 Series: AFC-3000 Automated Fraction Collector 11 Index Diverter Valve Assembly ........27 Adjust Height ..........33 Alignment ............. 44 Accessories Mount Assembly ........... 27 Optional ............74 Diverter Valve Connector ........19 Standard ............73 Drain Tubing ............. 29 Alignment ............
  • Page 88 UltiMate 3000 Series: AFC-3000 Automated Fraction Collector Service ............... 61 Check for Leaks ..........63 Normal-Phase LC ........15, 64 Clean Fraction Collector ....... 62 Firmware Update .......... 69 General Notes ..........61 Install Low-Flow Kit ........66 Operating Instructions ......... 1 Install Normal-Phase Kit ......

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