Results, - Beckman Coulter LH 780 Instructions Manual

I. Verify calibration by cycling each level of 5C
mode.
Procedure
A Ensure Apertures are Clean
If the instrument is shut down, and you routinely shut down the instrument for at least
30 minutes every 24 hours in LH Series Cleaner, cleaning is unnecessary.
If you routinely use LH Series Cleaner, but you are beginning calibration after processing
patient samples, shut down the instrument in the cleaning agent for 30 minutes before
proceeding.
CAUTION Possible flow cell damage could occur if you aspirate bleach. Do not aspirate bleach.
If you do not routinely use LH Series Cleaner, bleach the apertures before proceeding.
B Ensure the Instrument is Functioning Properly
1. Check the reagent containers for:
r
r
r
r
WARNING Biohazardous materials might be contained in the waste container and could cause
contamination unless handled with care. Avoid skin contact. Clean up spills immediately. Follow your
laboratory's protocol for safety measures.
2. Check the waste container for:
r
r
3. Perform daily startup.
4. In addition to verifying daily startup results, verify acceptable control results.
C Prepare the Instrument for Calibration
1. Ensure room temperature is stable and within the normal ambient temperature
range. If the average ambient room temperature changes more than 10°F from the
calibrating temperature, verify calibration and recalibrate (if necessary).
2. Ensure the blood detector is enabled.
3. Ensure the test mode is CBC.
4. Go to the Numeric Keypad.
5. Press
6. Cycle a sample of normal whole blood in Automatic aspiration mode as a prime.
PN 773023AC
Sufficient quantity
Not beyond expiration date
No precipitates, turbidity, particulate matter, or unusual color
Proper connections between the Diluter and the reagent containers.
Sufficient capacity
Proper connections.
PRIME
APERT
if necessary to activate pneumatics.
CALIBRATING CBC PARAMETERS WITH S-CAL
®
Cell control in Automatic aspiration
1
CALIBRATION
®
CALIBRATOR
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