Related Manuals for Beckman Coulter Microfuge 20 Series
Summary of Contents for Beckman Coulter Microfuge 20 Series
- Page 1 Instructions For Use Microfuge 20 Series Centrifuges B30162AA May 2013 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 Beckman Coulter, Inc. Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
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Page 3: Revision Status
Revision Status Initial Issue, 05/2013 Microfuge 20 Series Centrifuges Instructions for Use, version B30162AA B30162AA... - Page 4 Revision Status B30162AA...
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Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate the instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative. - Page 6 Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.
- Page 7 • If the instrument housing becomes damaged do not use the instrument. Contact Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at http://www.beckmancoulter.com). Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive.
- Page 8 Safety Notice Chemical and Biological Safety viii B30162AA...
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Page 9: Table Of Contents
Contents Revision Status, iii Safety Notice, v Introduction, xv CHAPTER 1: Description, 1-1 Introduction, 1-1 Centrifuge Function and Safety Features, 1-1 Centrifuge Function, 1-1 Models, 1-2 Safety Features, 1-3 Chassis, 1-3 Housing, 1-3 Door, 1-3 Rotor Chamber, 1-4 Drive, 1-4 Temperature Sensing and Control (refrigerated models only), 1-4 Controls and... - Page 10 Contents Entering Run Parameters, 2-4 Menu Key Sequence, 2-4 Setting Run Speed, 2-5 To set the speed, 2-5 Setting Run Time, 2-5 To set a timed run, 2-6 To start a continuous run, 2-6 To stop a continuous run, 2-7 Setting Run Temperature (Microfuge 20R refrigerated models only), 2-7 To set the run...
- Page 11 APPENDIX A: Installation, A-1 Introduction, A-1 Installing the Instrument, A-1 To install the centrifuge, A-2 Electrical Requirements, A-3 Test Run, A-5 APPENDIX B: Table of Hazardous Substance’s Name and Concentration, B-1 Beckman Coulter, Inc. Microfuge 20 Series Centrifuge Warranty Related Documents...
- Page 12 Illustrations Illustrations Microfuge 20 (A) and Microfuge 20R (B) Front View, 1-1 Microfuge 20R (A) and Microfuge 20 (B) Rear view, 1-2 Interior View of the Rotor Chamber, 1-4 Microfuge 20 and Microfuge 20R Control Panels, 1-5 Digital Display showing all possible displays, Microfuge 20R shown, 1-7 Tapered Sleeve Side View, 2-3...
- Page 13 Tables Tables Display Definitions, 1-7 Acceleration and Deceleration Rates by Rotor (in minutes:seconds), 2-9 Error Message Chart, 3-3 Troubleshooting Chart, 3-5 xiii...
- Page 14 Tables...
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Page 15: Introduction
Scope of Manual This manual is designed to familiarize you with the Beckman Coulter Microfuge 20 series centrifuges, their functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommends that you read this entire manual, especially the... - Page 16 ( CFC-Free Centrifugation To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Microfuge 20 series centrifuges. Symbols and Labels This section provides information for some labels and symbols appearing on the Microfuge 20 instrument housing.
- Page 17 Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative. Protective Ground This symbol is used to indicate a protective ground. This instrument must be properly grounded.
- Page 18 For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
- Page 19 Introduction Symbols and Labels China RoHS Caution Label This label and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. This logo indicates that this electronic information product contains certain toxic or hazarous elements, and can be used safely during its environmental protection use period.
- Page 20 Introduction Symbols and Labels CE Label This label indicates conformance to various directives set forth under European Union law. Consult IFU Label This label indicates that the Instructions for Use should be referred to for more information. RoHS Conform Label This label indicates that the product is compliant with the EU RoHS directive (2002/95/EC) pertaining to the Restriction of Hazardous Substances in Electrical and Electronic Equipment (EEE).
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Page 21: Description
CHAPTER 1 Description Introduction This chapter provides a brief physical and functional description of Beckman Coulter Microfuge 20 series centrifuges. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). -
Page 22: Models
1. Power switch 2. Power input Beckman Coulter Microfuge 20 series bench top centrifuges generate centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed specifically for use in this centrifuge, the instrument applications include: •... -
Page 23: Safety Features
Description Chassis Safety Features Microfuge 20 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Instrument safety features include: • An electromechanical door-locking mechanism to prevent operator contact with spinning rotors. -
Page 24: Rotor Chamber
Description Temperature Sensing and Control (refrigerated models only) Rotor Chamber The rotor chamber is shown in Figure 1.3. The drive shaft and rubber boot surrounding the drive shaft are visible in the chamber bottom. A gasket system around the chamber opening ensures sealing. -
Page 25: Controls And Indicators
Description Controls and Indicators Controls and Indicators Power Switch The power switch is located on the centrifuge back panel (see Figure 1.2). This two-position rocker switch ( , on; , off) controls electrical power to the centrifuge. NOTE The power must be turned on before the chamber door can be opened. Control Panel The control panel is mounted at an angle on the front of the centrifuge and includes system keys, programming keys, and digital displays (see... -
Page 26: Program Keys
Description Controls and Indicators START/STOP Pressing the START/STOP key causes a centrifuge run to begin. This key can also be used to abort a deceleration process and restart the centrifuge. The START/STOP key can be pressed to end a run. The centrifuge decelerates to a complete stop according to the preselected deceleration curve. -
Page 27: Name Rating Plate
The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge. -
Page 28: Microfuge 20 Nonrefrigerated Model Specifications
Description Microfuge 20 Nonrefrigerated Model Specifications Microfuge 20 Nonrefrigerated Model Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Speed Set speed to 15,000 rpm (in 100-rpm increments) min: 200 rpm actual rotor speed, ±50 rpm of set speed Speed control Speed display... -
Page 29: Microfuge 20R Refrigerated Model Specifications
Description Microfuge 20R Refrigerated Model Specifications Microfuge 20R Refrigerated Model Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Speed Set speed to 15,000 rpm (in 100-rpm increments) min: 200 rpm actual rotor speed, ±50 rpm of set speed Speed control Speed display... -
Page 30: Available Rotors
Description Available Rotors Available Rotors See the applicable rotor manual for information on rotor use, care and maintenance, and rotor accessories. Rotor Capacity Rotor Part Manual (´ g) Rotor Profile Description (mL) Number Number 24 ´ 2.0 FA241.5 = 82.0 mm 20,627 15,000 B30155... -
Page 31: Operation
CHAPTER 2 Operation Introduction This section contains operating procedures for the centrifuge, using the rotor designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power turned off ( ) when the centrifuge is not in use. -
Page 32: Run Procedure
Operation Run Procedure WARNING Risk of personal injury or property damage. The centrifuge must not be used in the vicinity of flammable liquids or vapors, and such materials should not be run in the centrifuge. During operation you should come within the 30 cm (12 in.) clearance envelope only to adjust the instrument controls, if necessary. -
Page 33: Tapered Sleeve Side View
Figure 2.1 Tapered Sleeve Side View NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com). -
Page 34: Entering Run Parameters
Operation Run Procedure Entering Run Parameters When the power is applied for the initial use (no previous runs), default values are displayed (see Figure 2.2). After the initial use, the parameters of the latest previous run are displayed when power is applied. When run parameters for a rotor have been entered, as described below, they are retained in the centrifuge memory. -
Page 35: Setting Run Speed
Operation Run Procedure Setting Run Speed The RCF value is determined by the rotor geometry and speed. The RCF and speed values, therefore, depend on each other. If one of the two values is entered, the other value will be set automatically. Centrifuge speed can be set for the maximum rated speed of the selected rotor. -
Page 36: To Set A Timed Run
Operation Run Procedure Continuous Run During a continuous run, the run time of the centrifuge is unlimited and must be stopped manually. The centrifuge accelerates during the continuous run until the set speed is reached. During centrifugation the TIME display begins counting up, when the rotor starts to spin. To set a timed run Press the key until the time unit flashes on the display. -
Page 37: To Stop A Continuous Run
Operation Run Procedure To stop a continuous run Press the key. START/STOP Deceleration begins immediately. The elapsed time shows during deceleration. The run ends. Setting Run Temperature (Microfuge 20R refrigerated models only) Run temperature can be set between –10 and +40°C. The typical operating range is from +2°C to 40°C, depending on the rotor and speed selected. -
Page 38: Rotor Code Selection
Operation Run Procedure Rotor Code Selection Select the installed rotor to allow for the correct RCF value to be selected and displayed. NOTE All of the available rotors for the Microfuge 20 and Microfuge 20R have the same rated speed of 15,000 RPMs. -
Page 39: Setting Acceleration And Deceleration Rates
Setting Acceleration and Deceleration Rates Microfuge 20 series centrifuges feature two acceleration rates — fast and soft (or slow), and two deceleration rates — fast and soft (or slow). See below for actual times of fast and soft acceleration and deceleration by rotor. -
Page 40: To Activate Only The Soft-Stop Function
Operation Run Procedure To activate only the Soft-stop function Press the key until the function flashes on the display. MENU Soft-stop/start Program mode ends if no other keys are pressed within 15 seconds. Press the cursor key until the is displayed. Soft stop on The soft-stop function is now activated. -
Page 41: Short Run (Quick Run) Function
Operation Run Procedure — Throughout the run, checks are made to ensure that the rotor does not exceed set speed. • The display indicates the actual rotor speed in RPMs. SPEED — The RCF can be displayed by pressing he cursor key. -
Page 42: To Perform A Quick Run On The Refrigerated Microfuge 20R
Operation Run Procedure Release the key. QUICK RUN • When the key is released, the centrifuge decelerates at maximum rate to QUICK RUN a standstill. • After the quick run and the centrifuge has come to a complete stop, the door unlocks automatically and the program that was set beforehand is displayed again. -
Page 43: To Change The Run Time During A Run
Operation Run Procedure To change the run time during a run Press the key until the time function flashes on the display. MENU • The last digit on the display flashes, indicating that the time can be raised or lowered TIME with the cursor keys. -
Page 44: Unloading
If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter Field Service at 1-800-742-2345 (U.S.A or Canada); outside the U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com). - Page 45 Operation Summary of Run Procedures Enter run parameters: — until RPM or RCF displays, use until correct speed displays. • Set run speed MENU — until time displays, use until correct time displays. • Set run duration MENU — until temperature displays, use •...
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Page 46: Saving And Using Programs
Operation Saving and Using Programs Saving and Using Programs The program is used to save or load certain recurring settings of the centrifuge. Ten separate programs can be saved and reused. NOTE The precooling program does not occupy any storage location and cannot be deleted. It is used only to cool the centrifuge without vessels. -
Page 47: To Run A Saved Program
Operation Saving and Using Programs To run a saved program Press the key. PROG Press the cursor key to scroll and select the program number to be run. Press until stops flashing. PROG Prog ## Close the centrifuge door and push firmly down on both sides of the door front until the clicking (latching) sound stops. - Page 48 Operation Saving and Using Programs 2-18 B30162AA...
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Page 49: Chapter 3: Troubleshooting
If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, gather as much information about the situation as you can: •... -
Page 50: To Clear The Problem Or Error
Troubleshooting User Messages Figure 3.1 Error message example In the event of a fatal error (for example, a defective door lock), a certain safety time will be counted down on the display. During this time, flash alternately on the display. When the time SAFE is up, will be displayed. -
Page 51: Error Message Chart,
Troubleshooting User Messages Table 3.1 Error Message Chart Error Number Problem Recommended Action Explanation 1 through 9, System error After rotor comes to a complete All of these errors stop stop, turn the power off (O), the centrifuge or then back on (I) to reset. cause it to decelerate brakeless. - Page 52 • Remove foreign matter from the opening of the door lock device • Check connections 90 through 99 Other errors • Provide sufficient water throughput (only water cooled centrifuges) NOTE Contact Beckman Coulter Field Service if error messages persist. B30162AA...
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Page 53: Other Possible Problems
Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service. Table 3.2 Troubleshooting Chart... -
Page 54: Accessing The Centrifuge In Case Of Power Failure
Troubleshooting Accessing the Centrifuge in Case of Power Failure Accessing the Centrifuge in Case of Power Failure If the facility power fails only momentarily, the centrifuge resumes operation when power is restored and the rotor returns to set speed. However, if the rotor comes to a complete stop the centrifuge run must be restarted when the power is restored. -
Page 55: Removing The Emergency Access
Troubleshooting Accessing the Centrifuge in Case of Power Failure Figure 3.2 Removing the Emergency Access Plug 1. Emergency access plug and position of the opening for the emergency door release Insert the supplied 4 mm (P/N B33985) Allen wrench horizontally into the hole and turn it counterclockwise until it stops. - Page 56 Troubleshooting Accessing the Centrifuge in Case of Power Failure B30162AA...
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Page 57: Care And Maintenance
CHAPTER 4 Care and Maintenance Introduction For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are discussed in CHAPTER 3, Troubleshooting. Refer to the applicable rotor manual and to Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories. -
Page 58: Maintenance
Care and Maintenance Maintenance Maintenance Preventive Maintenance The following procedures should be performed regularly to ensure continued performance and long service life of the centrifuge. Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass particles from broken sample tubes. a. - Page 59 Risk of personal injury or contamination. If you do not properly shield yourself while performing service, maintenance, and troubleshooting procedures, residual fluids in the instrument could injure or contaminate you. Beckman Coulter recommends barrier protection, such as appropriate safety glasses, lab coat, and...
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Page 60: Decontamination
Ethanol is a flammability hazard. Do no use it in or near operating centrifuges. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 61: Returning A Centrifuge
Returning a Centrifuge Before returning a centrifuge or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the authorization form and for packaging and shipping instructions. To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity. - Page 62 Care and Maintenance Supply List B30162AA...
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Page 63: Appendix A: Installation
APPENDIX A Installation Introduction This chapter contains instructions for installing and connecting the centrifuge. Check that required clearances and electrical power are available. WARNING Risk of personal injury. Each centrifuge weighs 13 kg/29 lb (nonrefrigerated model) or 32 kg/71 lb (refrigerated model.) DO NOT attempt to lift or move one of them without assistance from a lifting device or another person. -
Page 64: To Install The Centrifuge
Installation Installing the Instrument • Do not subject the centrifuge to thermal stress. For example, do not position the centrifuge near a source of heat. • Do not position the centrifuge where it receives direct sunlight (UV radiation). • The table or bench that the centrifuge is installed on must be stable and have a solid, flat surface. -
Page 65: Electrical Requirements
Installation Electrical Requirements Electrical Requirements See the Microfuge 20 Nonrefrigerated Model Specifications Microfuge 20R Refrigerated Model Specifications CHAPTER 1 for centrifuge electrical requirements. Make sure the voltage and frequency imprinted on the name rating plate affixed to the back of the centrifuge agree with the line voltage and frequency of the outlet used. -
Page 66: Dimensions Of The Refrigerated
Installation Electrical Requirements Figure A.2 Dimensions of the Refrigerated Centrifuge WARNING To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. -
Page 67: Test Run
The centrifuge must be plugged in and the power switch turned to the on position (I) before the door can be opened. Beckman Coulter recommends that you make a test run to ensure that the centrifuge is in proper operating condition following shipment. See... - Page 68 Installation Test Run B30162AA...
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Page 69: Table Of Hazardous Substance's Name And Concentration
APPENDIX B Table of Hazardous Substance’s Name and Concentration This materials declaration table (the Table of Hazardous Susbtance’s Name and Concentration) and the China RoHS Caution Label are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products”... - Page 70 有毒有害物质名称及含量的标识格式 Table of Hazardous Substance’s Name and Concentration 电子信息产品号码 EIP Part Number: 产品名称 Product Name: Microfuge 20 Series, Benchtop Centrifuge 产品型号Product Model Number: B31602 Microfuge 20 220V-240V/50 Hz B31606 Microfuge 20 IVD 220V-240V/50 Hz B31610 Microfuge 20R 220V-240V/50 Hz B31614 Microfuge 20R IVD 220V-240V/50 Hz 部件名称...
- Page 71 (2) years after delivery of the Microfuge 20 Series centrifuge (the product), to the original Buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
- Page 72 Beckman Coulter, Inc. Microfuge 20 Series Centrifuge Warranty Warranty-2 B30162AA...
- Page 73 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 한국어 Symbol Simbole Symbol символ Simbolo Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목 符号...
- Page 74 Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 Available in hard copy or electronic pdf by request. Available at www.beckmancoulter.com www.beckmancoulter.com © 2013 Beckman Coulter, Inc. All Rights Reserved...
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