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Summary of Contents for Beckman Coulter SX4400
- Page 1 Instructions For Use SX4400 Swinging Bucket Rotor For Use in the Beckman Coulter Allegra X-30 Series Centrifuges B01198AB October 2013 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 SX4400 Swinging Bucket Rotor PN B01198-AB (October 2013) Copyright © 2013 Beckman Coulter, Inc. Beckman Coulter and the stylized logo are trademarks of Beckman Coulter, Inc., and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
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Page 3: Revision History
Revision History This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released. Initial Issue, 04/2011 Rev AB, 10/2013 Links to the topics that changed are listed below: Chapter 1: Specifications The Rotor... - Page 4 Revision History B01198AB...
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Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to use this equipment. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative. - Page 6 Although rotor components and accessories made by other manufacturers may fit in the SX4400 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the SX4400 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer.
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Page 7: Table Of Contents
Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, and Note, v Safety Information for the SX4400 Rotor, v SX4400 Swinging Bucket Rotor, 1 Specifications, 1 Description, 2 Rotor, 2 Buckets and Accessories, 2 Preparation and Use, 5... - Page 8 Illustrations Illustrations The SX4400 Rotor and Accessories, 3 Installing the Rotor Yoke on the Centrifuge Drive Shaft, 6 Arranging tubes in the Rotor, 8 Seating Buckets on Yoke Pivot Pins, 9 viii...
- Page 9 Tables Tables Available Tube Adapters for the SX4400 Rotor, 3...
- Page 10 Tables...
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Page 11: Sx4400 Swinging Bucket Rotor
SX4400 Swinging Bucket Rotor Specifications Maximum speed Allegra X-30R 4700 rpm Allegra X-30 4200 rpm Density rating at maximum speed 1.2 g/mL Critical speed range 800 to 1200 rpm Relative Centrifugal Field at maximum speed at r (172 mm) Allegra X-30R 4255 ´ g r min Allegra X-30 3398 ´... -
Page 12: Description
1. Bucket Cover (392266) 2. Bucket (B01664) 3. Bucket O-ring (392621) This Beckman Coulter rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. The Rotor The SX4400 is a four-place swinging bucket rotor. This rotor carries adapters that allow centrifugation of a wide range of tube and bottle sizes (from 1.5-mL reaction vials to 400-mL... -
Page 13: The Sx4400 Rotor And Accessories
4. Tie-down Screw 2. Bucket Cover 5. Rotor Yoke 3. T-handle Rotor Wrench NOTE The latest information regarding labware and accessories used in Beckman Coulter rotors can be obtained at www.beckmancoulter.com. Table 1 Available Tube Adapters for the SX4400 Rotor Adapters Tubes... - Page 14 Bucket covers cannot be used with this tube/adapter combination. The maximum length of tubes used in the SX4400 buckets with covers is 125 mm for 250-mL bottles and 122 mm for all other tubes (when placed near the center of the adapter ).
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Page 15: Preparation And Use
Beckman Coulter. Use of other manufacturers’ components or accessories in the SX4400 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor. -
Page 16: Installing The Rotor Yoke
SX4400 Swinging Bucket Rotor Preparation and Use Installing the Rotor Yoke NOTE Before the first use of a new rotor, lubricate the pivot pins with Spinkote (306812). After the first use, apply Spinkote to the pivot pins approximately once a week and after cleaning or autoclaving. -
Page 17: Loading The Buckets
SX4400 Swinging Bucket Rotor Preparation and Use Loading the Buckets For runs at other than room temperature, refrigerate or warm the rotor and precool the centrifuge beforehand for faster equilibration. WARNING Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms. - Page 18 SX4400 Swinging Bucket Rotor Preparation and Use Figure 3 Arranging tubes in the Rotor 1. Pivotal Axis NOTE All buckets must be attached to the rotor for every run, whether loaded or empty. Never run the rotor with fewer than four positions filled Place covers on buckets, if applicable.
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Page 19: Operation
SX4400 Swinging Bucket Rotor Preparation and Use Figure 4 Seating Buckets on Yoke Pivot Pins CORRECT INCORRECT Pivot pins are seated in bucket pockets Pivot pins are not seated in bucket pockets 1. CORRECT 3. Pivot pins are seated in bucket pockets 2. -
Page 20: Run Speeds
Beckman Coulter highly recommends that you pretest these tubes in the appropriate labware in the SX4400 rotor, using water samples, to determine optimal operating conditions. -
Page 21: Care And Maintenance
• If any of these signs are evident, do not run the rotor. • Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center. Regularly check the condition of the tie-down screw (361367); if it is worn or damaged, replace it. -
Page 22: Cleaning
SX4400 Swinging Bucket Rotor Care and Maintenance Cleaning Wash rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor. Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues. -
Page 23: Decontamination
If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or †... -
Page 24: Sterilization And Disinfection
Use the minimum immersion time for each solution, per laboratory standards. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 25: Supply List
NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com or by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. Call Beckman Coulter Sales (1-800-742-2345 in the United States) for detailed information on ordering parts and supplies. -
Page 26: Other
SX4400 Swinging Bucket Rotor Supply List Other Adapters Table 1 Spinkote lubricant (2 oz) 306812 Silicone vacuum grease (1 oz) 335148 Rotor Cleaning Kit 339558 Beckman Solution 555 (1 qt) 339555 Rotor cleaning brush 339379 B01198AB... -
Page 29: Beckman Coulter, Inc
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery... - Page 30 Beckman Coulter, Inc. Benchtop Rotor Warranty Warranty-2 B01198AB...
- Page 32 Related Documents Allegra X-30 Series (B01145) Additional References Safety Chemical Resistances for Beckman Coulter • • Centrifugation Products (IN-175) Introduction • Use and Care of Tubes and Bottles (IN-192) • Description • Installation • Available in hard copy or electronic pdf by request.
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