Immunochemistry system, for in vitro diagnostic use (536 pages)
Summary of Contents for Beckman Coulter SlideMaker LH 700 Series
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S T A R T U P S H U T D O W N P O W E R O F F PN 4277299DE (January 2013) Manufactured by Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.
This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for your instrument.
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This document applies to the latest software listed and higher versions. When a subsequent software version changes the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for your instrument.
INTRODUCTION This introductory section contains the following topics: How to use your COULTER LH 700 Series System hard-copy manuals About this manual Online Help System Conventions HOW TO USE YOUR COULTER LH 700 SERIES SYSTEM HARD-COPY MANUALS Use the Getting Started booklet to see an overview of the system hardware and software. This document comes with your LH 700 Series System.
INTRODUCTION ABOUT THIS MANUAL ABOUT THIS MANUAL Your LH 700 Series SlideMaker Operator’ s Guide is a source of information for the day-to-day operation of your instrument. This information is organized as follows: Chapter 1, Use and Function Contains the intended use of the SlideMaker, a brief history of the methods used, the reagents, the hazards, and a short description of the major components.
CONTENTS WARNINGS AND PRECAUTIONS, ii REVISION STATUS, iii INTRODUCTION, v HOW TO USE YOUR COULTER LH 700 SERIES SYSTEM HARD-COPY MANUALS, v ABOUT THIS MANUAL, vi ONLINE HELP SYSTEM, vi CONVENTIONS, vi CONTENTS, vii FUSE AND FUNCTION, 1-1 INTENDED USE, 1-1 METHOD HISTORY, 1-1 SYSTEM COMPONENTS, 1-2 SlideMaker (Front View), 1-2...
CONTENTS SPECIAL REQUIREMENTS: HARDWARE, 2-1 Space and Accessibility, 2-1 Electrical Input, 2-2 Ambient Temperature and Humidity, 2-2 Air Conditioning, 2-2 Ventilation, 2-2 Drainage, 2-2 SPECIAL REQUIREMENTS: SOFTWARE, 2-3 INTERUNIT CONNECTIONS, 2-3 Fluid, Pressure and Vacuum Connections, 2-3 Power and Signal Cables, 2-5 OPERATION PRINCIPLES, 3-1 SLIDE AND SAMPLE FLOW, 3-1 Slide Request, 3-1...
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CONTENTS SLIDE SPECIFICATIONS, 4-3 Slide Dimensions, 4-3 25 mm x 75 mm Slide, 4-3 26 mm x 76 mm Slide, 4-3 Other Slide Parameters, 4-4 Material, 4-4 Color and Optical Properties, 4-4 Precleaning, 4-4 Boiling and Solubility, 4-4 Ribbon Specifications, 4-5 PERFORMANCE CHARACTERISTICS, 4-5 Precision, 4-5 Carryover, 4-6...
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CONTENTS USING SLIDES, CASSETTES AND BASKETS, 5-4 Loading Slides into a Cassette, 5-4 Procedure, 5-4 Loading Slide Cassettes, 5-6 Procedure, 5-6 Removing Slide Cassettes, 5-7 Procedure, 5-7 Locking a Slide Cassette, 5-8 Procedure, 5-8 Unlocking a Slide Cassette, 5-8 Procedure, 5-8 Loading Baskets, 5-9 Procedure, 5-9 Unloading Baskets, 5-10...
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CONTENTS TROUBLESHOOTING, 7-1 OVERVIEW, 7-1 EXPIRATION DATES AND SLIDE STORAGE CONDITIONS, 7-1 Temperature and Humidity Recommendations, 7-2 SLIDEJAMS OR DEBRIS, 7-2 Checking the Dryer, 7-2 Procedure, 7-2 Checking the Dryer Temperature, 7-4 Testing the SlideMaker Dryer and Heater, 7-5 Procedure, 7-5 Checking the Pusher Bars, 7-7 Procedure, 7-7 Checking the Shuttle, 7-9...
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Testing the SlideMaker Dryer and Heater, 7-24 Procedure, 7-24 Viewing SlideMaker Settings, 7-24 Procedure, 7-24 Viewing SlideMaker Errors on the SlideMaker, 7-25 Procedure, 7-25 SLIDEMAKER ERROR MESSAGES, 7-27 REFERENCES, REFERENCES-1 INDEX, INDEX-1 BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT TRADEMARKS PN 4277299DE...
FUSE AND FUNCTION INTENDED USE The Beckman Coulter LH 700 Series SM SlideMaker shown in the following figure is intended for use as an optional peripheral to the Beckman Coulter LH 700 Series System. The SlideMaker creates a blood smear on a clean microscope slide using a segment of the blood sample aspirated by the LH 700 Series.
FUSE AND FUNCTION SYSTEM COMPONENTS SYSTEM COMPONENTS SlideMaker (Front View) SlideMaker Screen The SlideMaker screen displays the SlideMaker status and error messages, and provides buttons and screens for you to interface with the SlideMaker. Power On/Off This button is illuminated when the SlideMaker power is on.
FUSE AND FUNCTION SYSTEM COMPONENTS Reset Switch The reset switch is illuminated when the SlideMaker is processing slides. When you press this switch, the SlideMaker immediately initiates a reset cycle. Regulators (SlideMaker) The regulators adjust the vacuum and pressure in the SlideMaker. Regulator 1 (REG1 or VAC1) adjusts 17.5 Hg vacuum and Regulator 2 (REG2 or VAC2) adjusts 6.5 Hg vacuum.
FUSE AND FUNCTION SYSTEM COMPONENTS Catch Tray This graphic illustrates one of the catch trays that needs to be cleaned regularly. WARNING Risk of personal injury and contamination. The processing areas may contain prepared smears and/or broken glass with biohazardous material. Handle with care and avoid skin contact. PN 4277299DE...
FUSE AND FUNCTION SYSTEM COMPONENTS Slide Cassette Input Queue The cassette input queue moves filled slide cassettes into position for retrieval of the slides. Once the slide cassette is empty, it is moved to the output queue. PN 4277299DE...
FUSE AND FUNCTION SYSTEM COMPONENTS Output Queue The cassette output queue stores empty slide cassettes until the operator can retrieve and refill the slide cassettes. Ejector The ejector pushes slides out of the cassette and onto the smear truck, one at a time. PN 4277299DE...
FUSE AND FUNCTION SYSTEM COMPONENTS Truck The truck: Moves to the Slide Ejector module. Picks up slides from the Slide Ejector module and deposits slides on the shuttle. Holds a slide in position for making a wedge smear. Places a spreader slide on the smear slide, moves the spreader slide through the drop of blood, and stops, allowing the blood to wick along the end of the...
FUSE AND FUNCTION SYSTEM COMPONENTS Dispense Module The Dispense Module includes a dispense probe and a probe wipe for measuring and positioning the drop of blood used for a smear. Pusher Bars The slide pusher bars push the wet slide from the shuttle onto the conveyor belts of the Dryer module.
FUSE AND FUNCTION SYSTEM COMPONENTS Main Fluidics The Main Fluidics module is a centralized source of pneumatic power, reagents, and waste collection for the Dispense module. Dryer The dryer receives a labeled, wet, smear slide from the pusher bars, dries the smear, and moves the smear to the Slide Elevator module.
FUSE AND FUNCTION SYSTEM COMPONENTS Basket Queue The basket queue moves the slide baskets Top view Rear track Front track from the rear (input) track to the front (output) track. Note: Once a basket has advanced to the slide loading position under the platen, do not manually remove it from this position.
SYSTEM COMPONENTS Printer Ribbon The printer ribbon is used to print the bar-codes on the labels. Needle Changes When your Beckman Coulter Representative installs your SlideMaker, minor changes to the LH 700 Series Needle module are required. 1-11 PN 4277299DE...
Possible system damage can occur if you uncrate the instrument, install it or set it up. Keep the instrument in its packaging until your Beckman Coulter Representative uncrates it for installation and setup. Your instrument is tested before it is shipped from the factory. International symbols and special handling instructions printed on the shipping cartons tell the carrier how to handle this electronic instrument.
INSTALLATION SPECIAL REQUIREMENTS: HARDWARE Figure 2.2 Space for Small Form Factor (SFF)-type computers 33 cm (13 in.) 25.4 cm (10 in.) 38 cm (15 in.) 44.5 cm (17.5 in.) 45.7 cm (18 in.) 68.6 cm (27 in.) 88.9 cm (35 in.) 30 cm (12 in.) 64.8 cm (25.5 in.)
INSTALLATION SPECIAL REQUIREMENTS: SOFTWARE The waste line of this instrument is connected to the waste line of the LH 700 Series analyzer. For information about the drainage requirements for the LH 700 Series analyzer, refer to the LH 700 Series Reference Manual. SPECIAL REQUIREMENTS: SOFTWARE To set up the software on this instrument: Ensure the proper date format is set up on the Workstation.
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INSTALLATION INTERUNIT CONNECTIONS A n a l y z e r L H S l i d e M a k e r V A C 3 0 p s i D i l u t e r w a s t e 3 0 p s i d i l u e n t c l e a n i n g...
Power and Signal Cables The following figure shows the interunit connections of the power and signal cables that are supplied with the instrument. Your Beckman Coulter Representative makes these connections when installing and qualifying your instrument for subsequent use. A n a l y z e r...
OPERATION PRINCIPLES SLIDE AND SAMPLE FLOW Slide Request The SlideMaker makes blood smears based on the criteria and rules set up for the sample on the Workstation. You, the operator, can also request a slide for a particular sample prior to running the sample by using the Make Slide field on the Add Test window.
CHOOSING SLIDES Beckman Coulter requires that you use the slides developed especially for the Beckman Coulter SlideMaker. Beckman Coulter slides have many features that will help ensure system reliability.
SPECIFICATIONS/CHARACTERISTICS PHYSICAL SPECIFICATIONS Check that the space is sufficient for the SlideMaker. The dimensions and requirements for accessibility are shown below: Figure 4.1 Space for tower-type computers 33 cm (13 in.) 25.4 cm (10 in.) 38 cm (15 in.) 44.5 cm (17.5 in.) 45.7 cm (18 in.)
350 W (Btu) maximum Recommended Reagents The LH 700 Series SlideMaker uses the diluent and cleaning agent from the LH 700 Series: LH Series Diluent ISOTON 4 Diluent Beckman Coulter CLENZ cleaning agent LH Series Cleaner Sample Volume Aspirated Volume 250 µL maximum Dispensed Volume 4µL ±0.5 µL...
SLIDE SPECIFICATIONS Slide Dimensions Use only Beckman Coulter slides. Beckman Coulter has a 25 mm x 75 mm x 1mm slide and a 26 mm x 76 mm x 1 mm slide. NOTE: The 26 mm x 76 mm x 1mm slide is available only for the Japanese market.
Note: Changes in slide length may require calibration of the SlideMaker. The 25 mm x 75 mm Beckman Coulter slide has two frosted bars at opposite ends of the same side of the slide. The 26mm x 76mm slide contains a painted end with two laterally opposing frost dots on the opposite end.
PERFORMANCE CHARACTERISTICS The performance characteristics for this instrument apply only to an instrument that has been properly maintained as indicated in the Beckman Coulter LH 700 Series documentation. The following performance characteristics identify the quality of blood smears produced by the SlideMaker.
‘feather edge’.” Within this study, Beckman Coulter found that in the vast majority of the test cases, no cells were found in the working area of the slide.
Go to Run Configuration and reset the Patient Samples to your laboratory’ s routine slidemaking setting. Stain the slide using the laboratory staining process. Review the working area of the slide for the presence of the cellular sub-component of the controls. If control sub-components are observed, contact your Beckman Coulter Representative. PN 4277299DE...
OPERATING PROCEDURES SLIDEMAKER SCREENS Main Menu Screen Use this screen to access the functions you want to perform. Routine Functions Press This Menu Item Pneumatic/Power Supplies View SlideMaker pneumatic and power supply levels. Advance Basket Advance a slide basket one basket position. Unlock Cassette Release the slide cassette.
VIEW ERROR EXIT COULTER LH SLIDE MAKER SLIDEMAKER SETUP Setting Up SlideMaker Ensure your Beckman Coulter Representative has enabled your SlideMaker. Select to display the System Setup window. Select to display the SlideMaker Setup window. Specify the smear dispense mode.
OPERATING PROCEDURES SLIDEMAKER SETUP Do not select unless your Beckman Coulter Representative has instructed you to do so. It is used to download SlideMaker software. Ensuring the SlideMaker is Enabled In order for SlideMaker to be enabled, the Communications window of System Setup and the SlideMaker tab from the Run Configuration window must be properly configured.
CAUTION Use of improper slides can damage the SlideMaker and affect the quality of the blood smear. Use only slides with the characteristics recommended by Beckman Coulter Inc. Open the box of slides and move the wrapping out of the way, then carefully place the box on a level surface.
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OPERATING PROCEDURES USING SLIDES, CASSETTES AND BASKETS Hold the cover with two fingers and move it back until it locks. Place the cassette on the slide loading stand. Note: New slides can be loaded on top of the 4-5 remaining slides found in the cassette.
OPERATING PROCEDURES USING SLIDES, CASSETTES AND BASKETS Place the slides in the cassette with the frosted bars facing the front opening of the cassette. Note: Inspect the slide at the top of the cassette and discard it if it is contaminated from handling.
OPERATING PROCEDURES USING SLIDES, CASSETTES AND BASKETS Position the cassette with the raised Input Output arrow pointing up and facing you. queue queue Place up to four filled slide cassettes, one at a time, into the input queue in front of the cassette pusher. Top view Removing Slide Cassettes Procedure...
OPERATING PROCEDURES USING SLIDES, CASSETTES AND BASKETS Locking a Slide Cassette Procedure Ensure: SlideMaker is ready to make smears. LH 700 Series analyzer is either processing or ready to process samples. Press EXIT until you reach the MAIN MENU screen. Press ROUTINE FUNCTIONS Press...
OPERATING PROCEDURES USING SLIDES, CASSETTES AND BASKETS Loading Baskets Procedure WARNING Risk of injury from movable part. Be careful when working with movable parts. Open the plastic basket cover on the White Top view uprights SlideMaker. Hold the needed basket with two fingers, one finger on each end of the basket.
A discussion of these factors follows. Slide quality: Beckman Coulter slides have been manufactured to maximize compatibility with your SlideMaker. They are double-washed to ensure cleanliness and specially packaged to reduce humidity and debris. Our slides have also been designed with strategically placed frosted areas to help prevent multiple slides from sticking together.
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If your laboratory observes carryover beyond what is described in Performance Characteristics, please contact your Beckman Coulter Representative. Specimen condition: Morphological artifacts on the smear could be the result of prolonged storage or inadequate mixing.
OPERATING PROCEDURES SHUTDOWN While the number of cells counted is not statistically representative of the sample leukocyte population, evaluation errors can be reduced by increasing the number of cells counted on a film as well as increasing the number of slides read on a particular sample. No interfering substances will be observed other than the ones experienced using the manual method.
OPERATING PROCEDURES SHUTDOWN Extended Shutdown (SlideMaker) Procedure Use the extended shutdown procedure if you are not going to operate the SlideMaker for 48 hours or more. This will prevent the reagents from drying out and forming precipitate. Clean the SlideMaker before shutting it down: If the SlideMaker will remain in the laboratory, first shutdown the SlideMaker.
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Cleaning and Replacing Air Filter Procedure CAUTION Risk of damage to the Power Supply. A dirty air filter can cause the Power Supply to overheat. Check the filter and clean as needed. If a SlideStainer is attached to your SlideMaker, remove the SlideStainer cover prior to performing the following procedure.
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Note: If the filter is torn or shredded, discard it and replace it with a new one. Order replacement filters from your Beckman Coulter Representative. Return the filter to its original location. Put the filter cover back on. If a SlideStainer is attached, replace the SlideStainer cover.
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Cleaning Blood Spills and Broken Slides Procedure WARNING Possible biohazardous condition. Clean up any blood spill or broken slide as quickly as possible. Handle with care. Avoid skin puncture. Dispose of all contaminated disposable cleaning materials and broken slides (sharps) in accordance with your local regulations and acceptable laboratory practices.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Unplug the power cord. Unlatch the front cover and raise it up completely. Clean up solid materials first by mechanical means (tongs or a brush). Clean up any spilled blood with paper towels and place into a biomedical waste container.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES 10. If the spill or broken slide was inside the SlideMaker: Close the cover. Plug in the power cord. Turn on the power. Press to turn on the SlideMaker. PN 4277299DE...
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Cleaning a Catch Tray Procedure CAUTION Possible biohazardous condition. Handle with care. Avoid skin puncture. Dispose of all contaminated disposable cleaning materials and broken slides (sharps) in accordance with your local regulations and acceptable laboratory practices. Use a cleaning solution capable of inactivating the Human Immunodeficiency Virus (HIV) and...
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Cleaning the Dispense Probe and Rinse Cup Procedure Perform this procedure on a daily basis if visual inspection of the dispense probe and rinse cup reveal debris or dried blood. Excessive debris or dried blood on external surfaces may affect the quality of the smears produced CHECKING SMEAR QUALITY..
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Unlatch the front cover of the SlideMaker and raise it up completely. PN 4277299DE...
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES WARNING Risk of injury from movable part. Be careful when working with movable parts. If necessary, manually move the smear truck and shuttle so they do not obstruct your view of the dispense probe. Wipe the dispense probe with a cotton-tipped applicator stick moistened with bleach solution.
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Close the cover. 10. Plug in the SlideMaker power cord. 11. Turn on the SlideMaker main power. POWER 12. Press to turn on the SlideMaker. Note: If problem with CHECKING SMEAR QUALITY persists, call your Beckman Coulter Representative 6-10 PN 4277299DE...
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Clearing a Clogged Dispense Probe and Rinse Cup Procedure Perform this procedure only if blood smears do not meet the characteristics as described in CHECKING SMEAR QUALITY. WARNING Possible biohazardous condition. Handle with care. Avoid skin puncture. Dispose of all contaminated disposable cleaning materials and broken slides (sharps) in accordance with your local regulations and acceptable laboratory practices.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Unlatch the front cover of the SlideMaker and raise it up completely. WARNING Risk of injury from movable part. Be careful when working with movable parts. If necessary, manually move the smear truck and shuttle so they do not obstruct your view of the dispense probe.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Pull out and turn (1/4 turn) the locking lever on the dispense probe. Lift the dispense probe out of the dispense probe mechanism. Disconnect the red-striped I-beam tubing from the dispense probe. WARNING Possible hazardous condition. Tubing can come in contact with biohazard materials.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES 10. Aspirate some of the bleach solution using a syringe and some flexible tubing. Attach the free end of the tubing to the dispense probe. 11. Push some of the bleach solution into and through the probe slowly, while holding the probe over some tissue.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES 12. Rinse the dispense probe thoroughly with distilled water using another syringe with tubing to push and pull the distilled water through the probe. 13. Dry the dispense probe with lint-free tissue. 14. Attach the SlideMaker red-striped I-beam tubing to the dispense probe.
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CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES 16. Wipe the rinse cup with another cotton-tipped applicator stick dipped in distilled water. Rinse cup 17. Push the dispense probe through the clearance hole in the dispense probe mechanism. 18. Turn the locking lever back (1/4 turn) and push in.
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20. Close the cover. 21. Plug in the SlideMaker main power. 22. Turn on the SlideMaker main power. POWER 23. Press to turn on the SlideMaker. Note: If problem with CHECKING SMEAR QUALITY persists, call your Beckman Coulter Representative. 6-17 PN 4277299DE...
CLEANING/REPLACEMENT PROCEDURES CLEANING PROCEDURES Priming/Backwashing SlideMaker Procedure Ensure: SlideMaker is ready to make smears. LH 700 Series analyzer is ready to process samples. Press EXIT until you reach the MAIN MENU screen. Press ROUTINE FUNCTIONS Press ROUTINE FLUIDICS Press PRIME REAGENT RESERVOIRS The SlideMaker status line displays BACKWASHING while the SlideMaker backwashes the sample lines and rinses the rinse trough.
CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Replacing SlideMaker Fuses Procedure Press to turn off the SlideMaker. Turn off the power. Unplug the power cord. Unscrew the threaded fuse cap, and pull it out of its hole. WARNING Risk of fire hazard. If you replace a fuse with a new fuse of a different type or rating, a fire can occur.
CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Turn on the power. Press to turn on the SlideMaker. REPLACING THE SLIDEMAKER LABEL ROLL Procedure Press to turn off the SlideMaker. Unlatch the front cover and raise it up completely. Remove the empty roll: Open the clip on the label supply. Label supply roll...
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CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Place the new roll. Place the supply and the take-up rolls on their retainer spools. Close the clip on the label supply. Note: The SlideMaker contains an arrow path to show the proper direction to thread the label tape. Thread the label tape through the Form form sensor, through the printer...
CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Close the cover. Press to turn on the SlideMaker. Print some test labels to ensure the new label roll is working properly. Replacing the SlideMaker Printer Ribbon Procedure Note: Replacing the printer ribbon is necessary after using approximately four rolls of labels. Press to turn off the SlideMaker.
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CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Remove the old printer ribbon. IMPORTANT Risk of breakage. The ribbon connecting the supply roll and take-up roll can break if you pull it too hard. Pull gently to separate the two rolls. If the connecting ribbon breaks, tape it to the take-up roll. Open the new printer ribbon package.
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CLEANING/REPLACEMENT PROCEDURES REPLACEMENT PROCEDURES Place the take-up roll on its retainer. Thread the ribbon around the tension bar. Wind the ribbon until you see the ribbon reach the tension bar. 10. Close the cover. 11. Press to turn on the SlideMaker.
If you suspect a problem in any of these areas, you can attempt to perform these functions before calling your Beckman Coulter Representative. If requested to do so by a Beckman Coulter Representative, you can also use the reset switch on the SlideMaker.
TROUBLESHOOTING SLIDEJAMS OR DEBRIS High humidity and high heat conditions appear to accelerate slide deterioration, while conditions of low humidity and low temperature appear to extend the shelf life of glass slides. Temperature and Humidity Recommendations Store at room temperature (15-30°C or 59-86°F) in a dry place (35-60% humidity). Whenever possible, allow closed slide containers to equilibrate to the working environmental conditions prior to opening the box.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS Remove all slide cassettes from the Input Output input and output queues. queue queue Top view Lift the cover to the dryer area. If a slide appears jammed in the dryer area, remove it with your hand and dispose of the slide.
TROUBLESHOOTING SLIDEJAMS OR DEBRIS Close the cover. If slides do not appear to be drying properly, Check the dryer temperature status. Test the dryer motor and heater to ensure they are working properly. Checking the Dryer Temperature Ensure: SlideMaker is ready to make smears. LH 700 Series analyzer is either processing or ready to process samples.
EXCEEDED. Make sure the ambient room temperature of your laboratory is within acceptable limits. If you change your room temperature and the problem persists, contact your Beckman Coulter Representative. Is less than 19°C (50°F), the SlideMaker status line displays LOW TEMPERATURE.
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HEATER ON. Check the Dryer Temperature screen to verify heater temperatures. If the heater fails to turn on, turn the SlideMaker off and call your Beckman Coulter Representative. HEATER OFF Turn off the SlideMaker heater. The SlideMaker status line displays HEATER OFF.
TROUBLESHOOTING SLIDEJAMS OR DEBRIS Checking the Pusher Bars Procedure WARNING Risk of injury from movable part. Be careful when working with movable parts. If you have not already done so, open the cover. Remove all slide cassettes from the Input Output input and output queues.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS If necessary, manually move the truck so it does not obstruct your view of the pusher bars. Manually move the pusher bars using your hand or a hemostat. Make sure to move the pusher bars all the way forward and backward.
TROUBLESHOOTING SLIDEJAMS OR DEBRIS Checking the Shuttle Procedure WARNING Risk of injury from movable part. Be careful when working with movable parts. If you have not already done so, open the cover. Remove all slide cassettes from the Input Output input and output queues.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS Remove slides, including jammed slides and debris, from shuttle, truck and pawls. Manually move the shuttle by gently sliding it or moving its belt inside instrument. Make sure to move the shuttle all the way along its path. If you suspect a vacuum problem, debris may exist on the shuttle or on the slide.
Replace the slides in the cassette. Return the full slide cassettes to the input queue. Top view Note: If your slides frequently jam in the cassette, call your Beckman Coulter Representative to adjust the slide opening. 7-11 PN 4277299DE...
TROUBLESHOOTING SLIDEJAMS OR DEBRIS Checking the Slide Ejector Module Procedure WARNING Risk of injury from movable part. Be careful when working with movable parts. If you have not already done so, open the cover. Remove all slide cassettes from the Input Output input and output queues.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS Manually move the pawls forward and backward to ensure debris is not blocking it. Return the full slide cassettes to the input queue. Close the cover. 7-13 PN 4277299DE...
TROUBLESHOOTING SLIDEJAMS OR DEBRIS Checking the Truck Procedure WARNING Risk of injury from movable part. Be careful when working with movable parts. If you have not already done so, open the cover. Remove all slide cassettes from the Input Output input and output queues.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS Manually move the truck by gently turning its gear on the right side of the instrument. Make sure to move the truck all the way along its path. If you suspect a vacuum problem, debris may exist on the truck or on the slide.
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TROUBLESHOOTING SLIDEJAMS OR DEBRIS Return the full slide cassettes to the input queue. Close the cover. 7-16 PN 4277299DE...
TROUBLESHOOTING SLIDE BASKET/CASSETTE SLIDE BASKET/CASSETTE Advancing a Slide Basket Procedure Note: This applies on SlideMaker option when running Automatic Mode Analysis. It does not apply if you have a SlideStainer. Ensure: SlideMaker is ready to make smears. LH 700 Series analyzer is either processing or ready to process samples. Press EXIT until you reach the MAIN MENU screen.
TROUBLESHOOTING LABEL PRINTING LABEL PRINTING Testing Label Printing Procedure Press EXIT until you reach the MAIN MENU screen. Press SPECIAL CYCLES. Press PRINT LABEL The SlideMaker status line displays PRINTING LABEL while the SlideMaker testing label is printing. Dummy label Discard 00001048 The SlideMaker prints test data on labels, applies the labels to slides and deposits the slides in slide baskets.
TROUBLESHOOTING LABEL PRINTING Press This DECREMENT INTENSITY Decrease the label intensity (make lighter). The minimum value is 1. After the value reaches 1, the label intensity jumps back to the maximum (32). INCREMENT INTENSITY Increase the label intensity (make darker). The maximum value is 32.
TROUBLESHOOTING REAGENTS AND REAGENT RESERVOIRS The SlideMaker uses this printer label intensity until it is restarted. If you want to save this printer label intensity as the new default, press SAVE PRINTER LABEL INTENSITY Press EXIT until you reach the MAIN MENU screen. Press RUN MODE to return to the RUN MODE screen.
TROUBLESHOOTING REAGENTS AND REAGENT RESERVOIRS Lowers its dispense probe into the rinse cup. Drains the diluent reservoir. Dries the aspiration line When the SlideMaker completes this processing, READY appears on the SlideMaker status line. Press until you reach the MAIN MENU screen. EXIT Press to return to the RUN MODE screen.
5 psi adjustment regulator on the rear of the SlideMaker. 30 PSI 30 psi adjustment control on the LH 700 Series Power Supply. RAW VAC The RAW VAC comes from the LH 700 Series Power Supply. It can only be adjusted by your Beckman Coulter Representative. 7-22 PN 4277299DE...
Press a key on the keypad. A row of # symbols appears on the screen, indicating which key was pressed. Repeat as needed for other keys. If a key fails to function properly, call your Beckman Coulter Representative. Press any key three times to exit to the SPECIAL FUNCTIONS screen.
Turn on the SlideMaker heater. The SlideMaker status line displays HEATER ON. Check the Dryer Temperature screen to verify heater temperatures. If the heater fails to turn on, turn the SlideMaker off and call your Beckman Coulter Representative. HEATER OFF Turn off the SlideMaker heater.
TROUBLESHOOTING HARDWARE SlideMaker software version for each microprocessor SlideMaker table versions for fluidics and screen display SlideMaker printer revision Slide counter - initialized to one, increments by one for each slide prepared; cannot be reset Cycle counter - initialized to one, increments by one each blood that is aspirated; cannot be reset Press EXIT...
1. Verify the SlideMaker is connected and on. SlideMaker Did Not r The SlideMaker is disconnected. 2. Acknowledge the error at the Workstation to reset the SlideMaker. Respond. 3. If the message persists, call your Beckman Coulter Representative. SlideMaker Log Ready SlideMaker Download Successful.
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The SlideMaker is disconnected. 2. Acknowledge the error at the Workstation to reset the SlideMaker. SlideMaker Communication 3. If the message persists, call your Beckman Coulter Representative. Problem SlideMaker Log No known problems identified, so Workstation received an unknown error If the message persists: continues operation.
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Wait until the last slide in the Dryer module is deposited in a slide basket. b. Turn off the SlideMaker. c. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. d. Call your Beckman Coulter Representative. PN 4277299DE 7-29...
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Note: You may not be able to retrieve all the slides. can insert the new label or d. Close the cover and call your Beckman Coulter Representative. ribbon roll. Note: The printhead is closed again when, with the cover closed, you reset the SlideMaker.
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SlideMaker. 3. If the message persists: a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-31...
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Note: You may not be able to retrieve all the slides. d. Inspect the area under the smear truck for a fallen slide and retrieve if possible. e. Close the cover and call your Beckman Coulter Representative BASKET (CORNERS FULL) SlideMaker Log 1.
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Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. BASKET (DID NOT TRANSFER BETWEEN SlideMaker Log 1.
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Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. BASKET (SLIDE NOT PLACED IN BASKET) SlideMaker Log 1.
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SEN34 Note: You may not be able to retrieve all the slides. t Basket present rear left, SEN35 d. Close the cover and call your Beckman Coulter Representative. t Basket present front left, SEN36 r SEN31, SEN32, SEN33, SEN34, SEN35 or SEN36 failed, indicating a corner has a slide basket when it does not.
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If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that 4. The SlideMaker stops. cassette extended sensor, SEN16, require slides appear in the Slide List. is blocked. c. Call your Beckman Coulter Representative. r SEN15 or SEN16 failed. CASSETTE (EXTEND TIMEOUT) SlideMaker Log 1. No new samples are aspirated.
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If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that 4. The SlideMaker stops. require slides appear in the Slide List. c. Call your Beckman Coulter Representative. CASSETTE (NO CASSETTE) SlideMaker Log 1. No new samples are aspirated.
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If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that sensor, SEN15, failed. require slides appear in the Slide List. c. Call your Beckman Coulter Representative. CASSETTE (RETRACT TIMEOUT) SlideMaker Log 1. No new samples are aspirated.
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SEN16, failed. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that 4. The SlideMaker stops. require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-39...
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3. Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. 4. Close the cover and call your Beckman Coulter Representative. 7-40 PN 4277299DE...
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. COMMUNICATION (PC TIMEOUT) SlideMaker Log 1. No new samples are aspirated.
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. 7-42 PN 4277299DE...
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If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. r Probe down sensor, SEN12, failed. c. Call your Beckman Coulter Representative. DISPENSE (PROBE NOT UP, SENSOR 11) SlideMaker Log 1.
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5. Close the cover and turn on the SlideMaker. 6. If the message persists: a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. 7-44 PN 4277299DE...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-45...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. 7-46 PN 4277299DE...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-47...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. DRYER (RETRACT, SENSOR 9) SlideMaker Log 1.
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SlideMaker. 5. If the message persists: a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-49...
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. EJECTOR (RETRACT, SENSOR 23) SlideMaker Log 1. No new samples are aspirated.
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9. If the message persists: 6. The SlideMaker stops. a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-51...
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9. If the message persists: 6. The SlideMaker stops. a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. 7-52 PN 4277299DE...
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Turn off the SlideMaker. r The slide registered sensor, SEN25, b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that failed. require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-53...
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Is Required. a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. 3. Call your Beckman Coulter Representative. FLUIDICS (DETECTOR 1) No, if SlideMaker Log...
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2. A smear may be made on one during require slides appear in the Slide List. of the samples in a reservoir. backwash c. Call your Beckman Coulter Representative. 3. Completed smears are dried and deposited in a slide basket. 4. The SlideMaker stops. FLUIDICS (DETECTOR 4)
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2. A smear may be made on one during require slides appear in the Slide List. of the samples in a reservoir. backwash c. Call your Beckman Coulter Representative. 3. Completed smears are dried and deposited in a slide basket. 4. The SlideMaker stops. FLUIDICS (DETECTOR 7)
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-57...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. 7-58 PN 4277299DE...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-59...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. 7-60 PN 4277299DE...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-61...
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Slide List. 2. The SlideMaker empties the vacuum accumulator until the c. Call your Beckman Coulter Representative. vacuum reservoir sensor, SEN51, indicates the reservoir is not full or until 30 seconds has elapsed. Note: The vacuum accumulator is then completely emptied over a fixed time period.
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. POWER (10V REFERENCE) SlideMaker Log...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. POWER (-15V SUPPLY) SlideMaker Log...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. POWER (24V SUPPLY) SlideMaker Log...
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. POWER (PROCESSOR B) SlideMaker Log...
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PRINTER (FAILURE) SlideMaker Log 1. No new samples are aspirated.
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If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PRINTER (OUT OF LABELS) SlideMaker Log 1. No new samples are aspirated.
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5. If the message persists: a. Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. PN 4277299DE 7-69...
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The reagent full sensor, SEN47, If this error appears when the DOWN. failed. reagent tank is pulling cleaning 4. If the message persists, call your Beckman Coulter Representative. agent: r All operations except shutdown continue normally. r Shutdown cannot be done until the reagent tank can fill with cleaning agent.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. SHUTTLE (NOT AT DISPENSE, SENSOR 7) SlideMaker Log 1. No new samples are aspirated.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. SHUTTLE (NOT AT SMEAR, SENSOR 6) SlideMaker Log 1. No new samples are aspirated.
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A sensor failed. require slides appear in the Slide List. The most likely condition is that when c. Call your Beckman Coulter Representative. the shuttle is near the printer position or the dispense position, the shuttle belt swings through the smear position sensor.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. TRUCK (FORWARD, SENSOR 2) SlideMaker Log The SlideMaker continues current At truck initialization: Note: Press any key on the SlideMaker screen to reset the alarm.
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Open the cover and remove the slides from the pusher bars area, Dryer module, and platen, and place them in a slide basket for staining. Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. PN 4277299DE 7-75...
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Note: You may not be able to retrieve all the slides. d. Close the cover and call your Beckman Coulter Representative. TRUCK (NOT AT CLEARANCE POSITION) SlideMaker Log 1.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. TRUCK (NOT AT PLACEMENT POSITION) SlideMaker Log 1. No new samples are aspirated.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. TRUCK (NOT CALIBRATED) SlideMaker Log The SlideMaker stops all The truck calibration values for the pick Note: Press any key on the SlideMaker screen to reset the alarm.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative TRUCK (NOT UP ERROR) SlideMaker Log 1. No new samples are aspirated.
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Turn off the SlideMaker. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. TRUCK (REVERSE, SENSOR 1) SlideMaker Log The SlideMaker continues current At truck initialization: Note: Press any key on the SlideMaker screen to reset the alarm.
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4. The SlideMaker stops. b. If AutoStop is ON, turn AutoStop OFF to resume operation. Samples that require slides appear in the Slide List. c. Call your Beckman Coulter Representative. TRUCK (VACUUM, SENSOR 3) SlideMaker Log 1. No new samples are aspirated.
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REFERENCES Lewis SM. Blood film evaluations as a quality control activity. Clinical and Laboratory Haematology. Koepke JA and England JM, eds. Blackwell Scientific Publications, London, 1990. NCCLS* document. Reference leukocyte differential count (proportional) and evaluation of instrumental methods. National Committe for Clinical Laboratory Standards.
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INDEX Download Unsuccessful., 7-27 Label Printer Communication DRYER (EXTEND, SENSOR 10), 7-45 Timeout, 7-68 DRYER (HIGH TEMPERATURE), 7-45 Label Printer Failure, 7-67 DRYER (NO SLIDE DETECTED ON Label Printer Initialization Failure, 7-67 PLATEN), 7-46 Label Printer Out Of Labels, 7-68 DRYER (PLATEN DID NOT COMPLETE Label Printer Out Of Ribbon, 7-68...
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INDEX REAGENT (WASTE FULL), 7-70 The Dryer, 7-31 Reagent Tank Not Full, 7-70 To Analytical Station Communication Lost Reports Processor ‘A’ VPP Failure, 7-66 (CRC Check Failed), 7-40 Reports Processor ‘B’ VPP Failure, 7-66 To Analytical Station Communication Reports Slide Dryer Transfer Mechanism Timeout, 7-40 (Shuttle to Dryer) Did Not Extend...
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INDEX WARNING intended use of Slidemaker, SLIDE FELL FROM DRYER, 7-31 intensity, 7-18, 7-19, 7-20 Workstation communications CRC decreasing on labels, 7-19 error, 7-41 increasing on labels, 7-20 Error Messages,tables of saving Slidemaker label intensity, 7-18 See also individual messages interunit connections, errors, 7-25...
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INDEX messages, 7-25 replacing ribbon (Slidemaker), 6-22 viewing on Slidemaker, 7-25 testing label printing, 7-18 method history of Slidemaker, version (Slidemaker), 7-24 motor, printer label functions, 5-1, 7-18, 7-19, 7-20 checking dryer, dcreasing Slidemaker printer testing Slidemaker dryer and intensity, 7-19 heater, 7-5, 7-24...
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INDEX checking, 7-14 unloading baskets, 5-10 unlocking slide cassette, 5-8, 7-17 use and function of Slidemaker, VAC1 and VAC2 Regulators, vacuum accumulator, 1-3, 7-21 draining in Slidemaker, 7-21 volume, sample aspirated for Slidemaker, waste container, specification, tubing connection, Workstation messages tables of, 7-27, 7-32, 7-36, 7-40, 7-43, 7-45, 7-50, 7-54, 7-57, 7-63, 7-67, 7-70,...
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BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT This Product contains software that is owned by Beckman Coulter, Inc. or its suppliers and is protected by United States and international copyright laws and international trade provisions. You must treat the software contained in this Product like any other copyrighted material.
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TRADEMARKS Beckman Coulter, the stylized logo, and COULTER are trademarks of Beckman Coulter, Inc. and are registered with the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders. Find us on the World Wide Web at: www.beckmancoulter.com...