Beckman Coulter Optima L Series Instruction Manual
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Beckman Coulter Optima L Series Instruction Manual

Preparative ultracentrifuges
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LL-IM-12
Optima
L Series
™
Preparative Ultracentrifuges

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  • Page 1 LL-IM-12 Optima L Series ™ Preparative Ultracentrifuges...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 3 CONVENTIONS Safety During Installation and/or Maintenance This instrument is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, should the instrument need to be moved, a Beckman Coulter Field Service representative must reinstall and relevel the instrument in its new location.
  • Page 4 Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use.
  • Page 5 LL-IM-12 August 2009 Instruction Manual © 2009 Beckman Coulter, Inc. Printed in the U.S.A.

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Instrument Certification ........vii Scope of Manual.
  • Page 8 Contents Page SECTION 2: RUN PREPARATION Power ..........2-1 Key Switch Positions .
  • Page 9 Illustrations Page Figure 1-1. The Control Panel ......... . 1-2 Figure 1-2.

  • Page 11: Introduction

    Introduction INSTRUMENT CERTIFICATION To assure full system quality, the Beckman Coulter Optima™ L series ultracentrifuges have been manufactured in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equip- ment requirements of applicable regulatory agencies.

  • Page 12: Conventions

    Introduction ➠ NOTE If the ultracentrifuge is used in a manner other than specified in this manual, the safety and performance of this instrument could be impaired. Further, the use of any equipment other than that intended for use by Beckman Coulter has not been evaluated for safety.

  • Page 13: Radio Inteterference

    Introduction WARNING Indicates high voltage or risk of electric shock. Refer servicing of all areas displaying this symbol to service personnel. TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.

  • Page 14: Recycling Label

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...

  • Page 15: Specifications

    Introduction SPECIFICATIONS CONTROL CHARACTERISTICS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Speed Set speed ....1000 rpm to maximum speed in increments of 100 rpm Speed control .
  • Page 16 5 microns (0.7 Pa) Instrument Classification ..S (uses all Beckman Coulter preparative rotors except Types 35 and 42.1 rotors with serial numbers 1299 or lower) Diagnostic Messages ..

  • Page 17: Preinstallation Requirements

    (Copies are also attached to the outside of the shipping container.) The following information is provided in case the ultra- centrifuge must be relocated. Contact your Beckman Coulter Field Service representative to adjust and level the instrument if it must be moved.
  • Page 18 In regions where the instrument is supplied with an unterminated cord, a plug that meets local electrical and safety requirements must be supplied. (Contact your local Beckman Coulter office for specific information regarding these requirements.) See the Table below for the required wire connections. Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
  • Page 19 Optima L series ultracentrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft).
  • Page 20 Introduction BIO-SAFETY LEVEL 3 INSTALLATION For laboratories with epoxy aggregate floors, such as BSL-3 labs, a non-invasive installation kit (PN 393862) is available. The kit, which consists of a high-strength adhesive tape, is CSA certified for use on epoxy aggregate floors only.

  • Page 21: Description

    Description The Optima L-100K or L-90K Preparative Ultracentrifuge, classified S, is used to generate centrifugal forces for the separation of particles. The instrument can be used with all available Beckman Coulter rotors for standard and zonal operation. This section describes the control panel and the instrument’s major components.

  • Page 22: Key Switch Positions

    Description KEY SWITCH POSITIONS A key interlock switch is used to select normal or zonal operation. The normal position is used for routine closed-door centrifugation and the zonal position for runs in which a zonal rotor is loaded and unloaded while spinning. CONTROL PANEL Figure 1-1 shows the front control panel.

  • Page 23: Displays

    Description Activation keys are designated to control specific ultracentrifuge functions. These are [ ], [ ] and [ ], located to the VACUUM START STOP right of the keypad. (All keys are discussed in more detail in the next section.) The keypad is used for entering numerical values.

  • Page 24: Run Status Indicators

    Description RUN STATUS INDICATORS Run indicators are provided in the form of green and red LEDs to indicate the status of the instrument. Figure 1-1 shows the location of the LEDs on the control panel and Table 1-1 describes the meaning of each.

  • Page 25: Door

    Description The meanings of the diagnostic messages, as well as appropriate actions to take, are discussed in Section 4: TROUBLESHOOTING AND MAINTENANCE DOOR The chamber door is made of high-strength structural steel. A solenoid interlock prevents it from being opened during operation. The door can be opened only by using the door handle and only if the power is on and the vacuum is off, with the chamber at atmospheric pressure.

  • Page 26: Vacuum System

    Description VACUUM SYSTEM The vacuum system is automatically activated when [ ENTER/RECALL and [ ] are pressed, or it may be turned on directly by pressing START the [ ] key. Three vacuum LEDs, labeled 750, 200, and <20, VACUUM are located in the middle of the upper display to indicate the level of chamber pressure in microns.

  • Page 27: Overspeed System

    SPEED diagnostic message will appear and the instrument will immediately shut down. DRIVE The Optima L series ultracentrifuges use a frequency-controlled, air- cooled direct-drive induction motor that requires no gears or brushes. In addition, the drive does not require an oil vacuum seal, external oil reservoir, or continuously operating damper.

  • Page 28: Name Rating Plate

    Description NAME RATING PLATE A name rating plate is affixed to the rear of the instrument. Always mention the serial number (located on the chamber door) and model number when contacting Beckman Coulter regarding your Optima L series instrument.

  • Page 29: Run Preparation

    Run Preparation This section explains how to use the control panel to enter run infor- mation. Refer to Figure 1-1 for the location of keys and indicators. POWER If the power to the instrument is off, flip the circuit breaker at the right side of the instrument on.

  • Page 30: Standard Operation

    Run Preparation ➠ NOTE As a safety feature, after you press ] and [ ], you must reset ENTER/RECALL START the key by turning it out of the zonal position then back into the zonal position. Then press ] and [ ] again to begin the ENTER/RECALL START...

  • Page 31: Parameter Keys

    Run Preparation • Press [ ] once to clear a numerical entry. • Press [ ] twice to exit from the editing mode. The display will stop blinking and the actual values will reappear. • Press [ ] once to clear a diagnostic message (see Section 4 for more information.) Use the [ ] key to enter or display values as follows.
  • Page 32 Run Preparation 3. Press another parameter key or [ ] to save the set ENTER/RECALL speed. After [ ] and [ ] have been pressed, the set speed is ENTER/RECALL START checked against the overspeed disk at 1000 rpm. If the speed setting is greater than that allowed by the overspeed disk, the run will continue, but the rotor speed will be adjusted to the maximum speed permitted by the disk.
  • Page 33 Run Preparation When [ ] and [ ] have been pressed, the instrument ENTER/RECALL START checks to make sure that a value has been entered in the TIME ω display, that a value for t has been entered in the speed display, or that the HOLD mode has been selected.
  • Page 34 Run Preparation ω t indicator will remain on until a different run mode is selected ω as a reminder that the instrument is still in the t mode. The accu- ω mulated t from the previous run will automatically return to zero when [ ] and [ ] are pressed to begin a new run.

  • Page 35: Programmed Operation

    Run Preparation The instrument has two acceleration profiles: maximum (MAX) and ACCEL (Acceleration) slow. Press the [ ] key repeatedly to toggle between the settings. ACCEL The run status indicator for the selected setting will light. The instrument has three deceleration profiles: maximum, slow, and DECEL (Deceleration) no brake.

  • Page 36: Activation Keys

    Run Preparation To select a program for use: 1. Press [ PROG 2. Press the required program number. 3. Press [ ] and [ ENTER/RECALL START To modify a parameter during the run without changing the program, change the setting as described above and then press [ ENTER/RECALL Do NOT press [ ].

  • Page 37: Table 2-1. Vacuum Leds

    Run Preparation Table 2-1. Vacuum LEDs Pressure 750 LED 200 LED <20 LED 750 or above blinking 220 to 750 blinking 20 to 200 blinking <20 After you have pressed [ ] and [ ], the instrument ENTER/RECALL START ω checks to see if there is an entry in the TIME display, or if t or HOLD has been selected.

  • Page 38: Delayed Start Program Function

    Run Preparation DELAYED START PROGRAM FUNCTION The delayed start program function allows you the convenience of delaying the start of a run so that it begins and ends at the time you require—without you having to be there to start the run. For example, if you require a four-hour run to end at 8:00 a.m., you can program the instrument to hold the rotor at a specified temperature and time at zero speed and then automatically begin the run at 4:00 a.m.

  • Page 39: Editing Settings During The Delayed Start Run

    Run Preparation The word “delay” will appear in the SPEED display and the second program number you selected will appear in the [ PROG display. When the time in program 0 reaches zero, the second program will begin automatically. Example: To run programs 0 and 3: Press [ ], [ ], [...

  • Page 41: Run Procedure

    This section summarizes standard, programmed, and zonal operation as well as the delayed start function. Use any of the acceptable Beckman Coulter rotors, consulting the applicable rotor manual and the Rotors and Tubes Manual (LR-IM) for instructions on preparing the rotor and accessories for centrifugation. For fast temperature equilibration, refrigerate or warm the rotor to the required temperature before the run.

  • Page 42: Standard Operation

    Run Procedure The power must be turned on and the vacuum system turned off in order to open the chamber door. STANDARD OPERATION 1. With the power on, open the chamber door, install the rotor, and close the chamber door. Press [ ] if you want to evacuate VACUUM the chamber now.

  • Page 43: Delayed Start Program Function

    Run Procedure 3. Press [ ] and [ ] to begin the run. (The vacuum ENTER/RECALL START system is automatically activated now, unless you pressed ] earlier.) VACUUM Any of the run conditions can be changed while a programmed run is in progress.

  • Page 44: Zonal Operation

    Run Procedure ZONAL OPERATION WARNING In zonal operation, the operator is unavoid- ably exposed to rotating machinery. For safety, the operator must be properly instructed and qualified. Guard against accidentally dropping objects, such as pens, pencils, or hemostats into the chamber. Loose lab coats, neckties, scarves, and long necklaces should not be worn while operating in the zonal mode.

  • Page 45: Acceleration

    Run Procedure ➠ NOTE If you are performing consecutive zonal runs: at the end of each zonal run, the centrifuge will automatically reset to the normal mode. To reconfirm selection of the zonal mode, you must turn the key left to right (clockwise) from the zonal position to the normal position and then back to the zonal position.

  • Page 46: Unloading

    Run Procedure 10. When the SPEED setting indicates that the rotor is spinning at 2000 rpm, a series of beeps will sound; press [ ] to vent VACUUM the chamber. Then open the chamber door. UNLOADING 11. Unload the rotor according to the instructions in the zonal rotor manual.

  • Page 47: Troubleshooting And Maintenance

    Troubleshooting and Maintenance This section lists possible malfunctions, together with probable causes and corrective actions. Maintenance procedures are also described. For any problems not covered here, contact Beckman Coulter Field Service for assistance. ➠ NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accesso- ries, before requesting service by Beckman Coulter Field Service.

  • Page 48: Retrieving Your Sample

    Troubleshooting and Maintenance RETRIEVING YOUR SAMPLE If a power failure lasts for several hours, it may be necessary to retrieve your sample from the rotor. (A rotor decelerating without the brake may take hours to come to a complete stop.) To gain access to the rotor chamber under these circumstances, you will need to remove the control head cover and the front panel to disengage the door lock.

  • Page 49: Figure 4-1. Removing The Control Head Cover

    Troubleshooting and Maintenance Access to Cover Latch Cover Latch Remove Key Figure 4-1. Removing the Control Head Cover WARNING After removing the panel, listen carefully for any sounds coming from the drive. Then touch the fan housing (Figure 4-3) to feel if it is vibrating and listen again.

  • Page 50: Figure 4-2. Depressing The Latch To Loosen The Front Panel

    Troubleshooting and Maintenance Top Panel Tabs Front Panel Access Port Front Panel Tabs Figure 4-2. Depressing the Latch to Loosen the Front Panel WARNING NEVER try to slow or stop the rotor by hand. 6. Push down on the interlock pin (Figure 4-3) with your finger while opening the chamber door with your free hand.

  • Page 51: Figure 4-3. Interior View Of Instrument

    Troubleshooting and Maintenance Spring-loaded Interlock Pin Door Lock System Vacuum Solenoid Screw Fan housing Figure 4-3. Interior View of Instrument (Door Removed) 8. Lift the front edge of the top panel a few inches and insert the upper edge of the front panel under it. Push down gently to engage the tabs.

  • Page 52: Diagnostic Messages

    The run will come to a stop. See Table 4-1 to determine the nature of the error or problem, possible causes, and recommended corrective actions. If no user action is indicated, or the error persists, call Beckman Coulter Field Service for assistance.

  • Page 53: Cleaning

    Loss of power during run Check TIME display; run may need to be restarted or aborted. Microprocessor malfunction or loss of No user action. Call Beckman Coulter Field Service. program memory CLEANING ➠ NOTE Before using any cleaning or decontamination...

  • Page 54: Decontamination

    See publication IN-175 for more information regarding chemical resistance of instrument and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the instrument, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 55: Supply List

    Troubleshooting and Maintenance SUPPLY LIST Call Beckman Coulter Sales (1-800-742-2345 in the United States) or your local Beckman Coulter office, or refer to Ultracentrifuge Rotors, Tubes, & Accessories catalog (BR-8101, available at www.beckman- coulter.com) for information on ordering parts, supplies, rotors, and accessories.
  • Page 57 Beckman Coulter personnel. portation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all trans- 4. The drive unit was installed by a Beckman Coulter Field portation charges. Service representative.
  • Page 58 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com ©2009 Beckman Coulter, Inc. All rights reserved...

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Optima l-100kOptima l-90k

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