Beckman Coulter CytoFLEX SRT Manuals

Beckman Coulter CytoFLEX SRT Manuals

Manuals and User Guides for Beckman Coulter CytoFLEX SRT. We have 1 Beckman Coulter CytoFLEX SRT manual available for free PDF download: Instructions For Use Manual

Beckman Coulter CytoFLEX SRT Instructions For Use Manual

Beckman Coulter CytoFLEX SRT Instructions For Use Manual (393 pages)

Cell Sorter

Brand: Beckman Coulter | Category: Laboratory Equipment | Size: 25 MB

Table of Contents

Table of Contents

16
- Revision History
  3
- Alerts for Warning, and Caution
  7
- Safety Notices
  7
- Safety Precautions
  8
- General Safety
  9
- Instrument Safety Precautions
  9
- Drop Drive Voltage
  11
- Electrical Safety
  11
- Stream Charge
  11
- Deflection Plate Arcing
  12
- Deflection Plates
  12
- Laser Power Supplies
  12
  - Table of Contents
    16
Illustrations

25
- About this Manual
  30
- How to Use Your Manual
  30
- Introduction
  30
- Overview
  30
- Conventions Used
  31
- Graphics
  32
- CHAPTER 1 System Overview
  
  33
  - Main Components
    35
  - Sort Overview Diagram
    35
  - Main Components
    36
  - Fluidic Flow
    37
  - Fluidics System
    37
  - Sheath Tank
    37
  - Shutdown Fluid
    37
  - Fluidics Cart
    38
  - Fluidics Cart
    40
  - Connector Color Convention
    41
  - Sheath Tank
    41
  - Shutdown Fluid Container
    42
  - Shutdown Fluid Container
    43
  - Waste Container
    43
  - Fluidics Module
    44
  - Waste Container
    44
  - Interconnections on the Right Side of Sorter
    45
  - Sample Station
    45
  - Sample Station
    46
  - Optical Components
    48
  - Cell Illumination
    49
  - Forward Scatter
    49
  - Laser Beam Shaping
    49
  - Side Scatter and Fluorescent Light
    49
  - Fluorescent Light
    50
  - Forward Scatter Collection
    50
  - Side Scatter
    50
  - Side Scatter and Fluorescent Light Collection
    50
  - Light Path through the WDM with a Single Port
    51
  - Optical Filter Mounts
    51
  - Wavelength Division Multiplexer (WDM)
    51
  - Optical Filter Mount (Top)
    52
  - Optical Filter Mount Labeled with the Band-Pass
    52
  - Optical Filter Mount with Optical Filter
    52
  - Cytoflex SRT
    53
  - WDM Optical Filter Mount Color Codes
    53
  - Optical Fiber
    54
  - Cell Sorting
    55
  - Sort Chamber
    55
  - Overview of Sort Chamber
    56
  - Sort Chamber [With Side Stream Illumination Source Removed]
    57
  - Stream Camera and Droplet Monitor
    57
  - Deflection Plates
    58
  - Droplet Status
    58
  - Nozzle Module
    59
  - Waste Catcher
    60
  - Cyclone Movement System
    61
  - Two 5 Ml Tubes and Two 15 Ml Tubes
    61
  - Four 5 Ml Tubes
    62
  - Standard 96-Well Plate
    62
  - Sample Temperature Control
    63
  - Interconnection Adapters
    64
  - Connecting Water Bath to the Sorter
    65
  - Aerosol Evacuation System
    66
  - Temperature Control Setting
    66
  - Aerosol Evacuation System
    67
  - Aerosol Evacuation Vents
    67
  - Instrument Electronics
    68
  - System Connections
    68
  - Power and Signal Cable Connections
    69
  - Power and Signal Cable Connections
    70
  - Fluid Harness Connections
    71
  - Overview of Sorter Connections
    71
  - Dimensions
    72
  - Instrument Specifications
    72
  - Sorter
    72
  - Acoustic Noise Level
    73
  - Electrical Ratings
    73
  - Installation Category
    73
  - Maximum Altitude
    73
  - Pollution Degree
    73
  - Sorter
    74
  - Performance Characteristics
    75
  - Material Safety Data Sheets (SDS/MSDS)
    76
- Operation Principles
  33
- Overview
  33
- Product Description
  33
- CHAPTER 2: Using the Cytexpert SRT Software
  
  77
  - Overview
    77
  - Launching the Software
    77
  - Main Software Screen
    77
  - Start Page
    78
  - Acquisition Screen
    79
  - Acquisition Screen Navigation
    80
  - Collection
    81
  - Acquisition/Sorting Control
    83
  - Tube Management
    84
  - Plot Area
    86
  - Status Bar
    87
  - Analysis Screen
    87
  - Drawing Controls Toolbar (Top of Screen)
    87
  - Compensation Experiment Screen
    88
  - QC Experiment Screen
    90
  - QC Report Screen
    90
  - QC Experiment Screen
    91
  - QC Screen Navigation
    91
  - Software Menu
    92
  - Software Menu Tree
    92
  - QC Software Menu Tree
    92
  - Acquisition and Analysis Screen Menu
    93
  - Cytexpert Default Software Option
    93
  - Cytexpert User Management Software Option
    93
  - User Management
    97
  - User Manager (Card View)
    97
  - User Manager (Grid View)
    98
  - Creating, Deleting, and Modifying Users in User Manager
    99
  - Unlocking a User Account
    101
  - Resetting a User Password
    102
  - Changing a User Password
    103
  - Forgot Username or Password
    104
  - Role Management
    106
  - Role Manager
    107
  - Creating, Deleting, and Modifying User Roles in Role Manager
    108
  - Account Policies
    111
  - Account Policies - Password Policy
    111
  - Account Policies - Account Lockout Policy
    112
  - Operation Log
    113
  - Account Policies - Application Inactivity Policy
    113
  - Viewing and Exporting Experiment Operation Logs
    114
  - Viewing and Exporting System Operation Logs
    116
  - Viewing and Exporting User Management Operation Logs
    118
  - Graphic and Gating Styles
    119
  - Plots
    119
  - Gates
    120
  - Collection Device Library
    121
  - Default Collection Device Library
    122
  - Sort Mode Library
    123
  - Sort Mode
    123
  - Sort Precision Level
    124
  - Drop Envelope
    125
  - Sort Precision Modes Applied to the same Sort Stream
    125
  - Sort Guard Band
    126
  - Sort Stream Precedence
    127
  - Sort Decision with Different Modes Applied (Sort Guard Band 25%)
    127
  - Adding a Sort Mode
    128
  - Deleting a Sort Mode
    131
  - Software Settings
    133
  - Language Settings
    139
- CHAPTER 3 Daily Startup
  
  140
  - Overview
    140
  - Pre-Startup Inspection
    140
  - System Connections Inspection
    140
  - Checking Fluid Levels
    141
  - Power Cable
    141
  - Turning on the Instrument
    142
  - Logging into the Software
    143
  - Sheath Status
    146
  - Waste Status
    146
  - Shutdown Fluid Status
    146
  - System Startup Program
    147
  - Selecting Experiments from the Start Page
    157
- CHAPTER 4: Instrument Quality Control and Standardization
  
  158
  - Overview
    158
  - Quality Control
    159
  - Preparing the QC Sample
    160
  - Required Materials
    160
  - Cytoflex Daily QC Fluorospheres Preparation Process
    160
  - Cytoflex Ready to Use Daily QC Fluorospheres Preparation Process
    161
  - Importing Lot-Specific Target Values
    161
  - Collecting QC Data
    167
  - Confirming Results
    170
  - QC Result Manager
    177
  - Standardization
    178
  - Preparing the Standardization Sample
    178
  - Required Materials
    179
  - Preparation Process
    179
  - Generating Target Median Values
    179
  - Adding a New Standardization Item
    186
  - Performing the Standardization
    190
  - Applying the Standardized Acquisition Settings
    193
  - Standardization Target Library
    196
- CHAPTER 5: Sorting
  
  199
  - Overview
    199
  - Sort Calibration (Auto Drop Delay)
    200
  - Default Amplitude Setting (Optional)
    206
  - Break-Off Point Position Reference
    206
  - Setting Drop Delay Manually (Optional)
    207
  - Creating an Experiment
    218
  - Changing the Tube Name
    219
  - Setting the Channel and Label
    220
  - Creating Plots and Gates
    223
  - All Gates - Example Experiment
    231
  - Circular Gating Logic - Example Experiment
    231
  - Creating and Adjusting Auto Gates
    233
  - Turning Auto Recalculate On/Off
    235
  - Adjusting Autogate Movement and Extent
    236
  - Movement - Default Setting
    236
  - Movement - Max Setting
    237
  - Extent - Default Setting
    237
  - Configuring Acquisition Settings
    238
  - Laser Settings
    238
  - Extent - Maximum Setting
    238
  - Laser Setting Window
    239
  - Target Power Ranges in the Laser Setting Screen
    239
  - Adjusting the Gain
    240
  - Adjusting the Threshold
    242
  - Setting Collection Conditions
    244
  - Setting Sample Flow Rate
    245
  - Setting Mixing Speed
    246
  - Setting Plot Display Conditions
    247
  - Setting Customized Parameters
    248
  - Setting Custom Statistics
    249
  - Load Sample and Record Data
    253
  - Before Running Samples
    253
  - Setting up Violet Side Scatter (VSSC) Channel
    254
  - Verifying, Selecting, Editing, and Creating Detector Configuration
    258
  - Sampling and Collecting Data
    261
  - Sorting
    265
  - Setting up Tube Sorting
    265
  - Setting up Plate/Slide Sorting
    271
  - 96-Well Deep Well Plate
    272
  - Stop Criteria for Sorting
    278
  - Calibrating the Sort Collection Device
    279
  - Manual Side Stream Calibration (Optional)
    287
  - Adjusting Tube Position (Optional)
    291
  - New Output Holder
    291
  - Starting and Monitoring a Sort
    292
  - During a Sort
    292
  - Viewing Sorter Status
    293
  - Side Stream Monitor
    294
  - Auto Maintain
    295
  - Viewing Sorting Statistics
    300
  - Sort Statistics
    300
  - Pause Sorting and Resume Sorting
    302
  - Additional Information for Plate Sorting
    302
  - Index Sorting
    303
  - Sort Report
    306
  - Creating a Sort Report
    306
  - Designing a Report
    307
  - Sort Report Setting-Tube Experiment
    307
  - Report Output
    308
  - Sort Report Setting-Plate Experiment
    308
  - Output Options on Sort Report Toolbar
    308
  - Additional Information for Aseptic Sorting
    309
  - Exporting Data
    309
  - Exporting FCS Files
    315
  - Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
    318
  - Importing and Exporting Instrument Settings
    319
  - Importing and Exporting Compensation Settings
    321
  - Printing Graphics
    322
  - Saving the Experiment
    324
  - Concluding the Experiment
    324
- CHAPTER 6 Compensation
  
  325
- CHAPTER 7: Data Review
  
  345
  - Overview
    345
  - Copying Experiments and Importing Data
    345
  - Copying a Previously Acquired Experiment
    345
  - Importing Previously Acquired Data
    345
  - Setting the Plots and Statistics
    347
  - Creating Histogram and Dot Plot Overlays
    348
  - Calculating Sample Concentration
    350
  - Adjusting Compensation Settings
    351
  - Statistics Setting
    351
  - Exporting Results
    352
- CHAPTER 8: Shutting down the System
  
  353
  - Overview
    353
  - Running the Daily Clean
    353
  - Running Shutdown Program
    354
  - System Shutdown
    354
  - Long Term Shutdown
    359
  - Cleaning During Shutdown
    369
  - Turning off the Power
    370
  - The Sorter)
    371
- CHAPTER 9: Troubleshooting
  
  372
  - Overview
    372
  - Laser Related Hazards
    372
  - Laser Beam Hazards
    373
  - Laser Warning Labels
    374
  - Laser Warning Label on the Laser Optical Bench
    374
  - Hazard Labels and Locations
    375
  - Laser Warning Label Within the Optical Bench
    375
  - Biohazard Label and Location
    376
  - Biohazard Label on the Fluidics Cart and Waste Container
    376
  - Biohazard Label on the Sample Station and Sort Chamber
    376
  - Electrical Shock Hazard Label and Location
    377
  - Caution Labels and Location
    377
  - Electrical Shock Hazard Label
    377
  - Label on the Sort Protection Door
    378
  - Caution Label on the Sample Station
    378
  - Pinch Hazard Labels and Location
    379
  - Disposal Precaution
    380

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