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Summary of Contents for Beckman Coulter GH-3.8
- Page 1 Instruction For Use GH-3.8 Swinging Bucket Rotor In Beckman Coulter Allegra 6 Series, GS-6 Series, Spinchron Series, and GP Series Centrifuges PN GS6-TB-003MD June 2018 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 GH-3.8 Swinging Bucket Rotor In Beckman Coulter Allegra 6 Series, GS-6 Series, Spinchron Series, and GP Series Centrifuges PN GS6-TB-003MD (June 2018) © 2018 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc.
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Page 3: Safety Notice
If any of these signs are evident, do not run the rotor. Contact your Beckman Coulter representative for information bout the Field Rotor Inspection Program and the rotor repair center. To reduce the potential for corrosion, clean buckets or carriers thoroughly immediately following a tube or well plate breakage. - Page 4 Beckman Coulter. Use of other manufacturers’ components or accessories in the rotors may void the rotor warranty and should be prohibited by your laboratory safety officer. If tubes, microplates, or other labware made by manufacturers other than Beckman Coulter are used, reduce rotor speed to prevent breakage.
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Page 5: Alerts For Danger, Warning, Caution, Important, And Note
Safety Notice Alerts for Danger, Warning, Caution, Important, and Note Alerts for Danger, Warning, Caution, Important, and Note DANGER DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury. WARNING WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury. - Page 6 Safety Notice Alerts for Danger, Warning, Caution, Important, and Note PN GS6-TB-003MD...
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Page 7: Table Of Contents
Contents Safety Notice, iii Safety Notice, iii Alerts for Danger, Warning, Caution, Important, and Note, v GH-3.8 Rotor, 1 Specifications, 1 Description, 3 Rotor, 3 Buckets and Accessories, 3 Modular Disk Adapters, 3 Bottle Adapters, 4 Blood Bag Cups, 4... - Page 8 Contents Buckets, 33 Micro Plus Plate Carriers (Including Base and Pad), Blood Bag Cups, and Bucket Covers, 33 Modular Disk Adapters, 33 Aerosolve Cannisters, 34 Decontamination, 34 Sterilization and Disinfection, 34 Tube Breakage, 35 Aerosolve Cannisters, 35 SST or Corvac Tubes, 35 Returning a Rotor, 36...
- Page 9 The Bails (Vertical Supports) of the GH-3.8 (and GH-3.7) Modular Disk Adapters and the J6 Series Adapters are Not the Same. Bails for the GH-3.8 are straight and end in a disk; bails for the J6 have curved ends. Do not intermix adapters (or disks from adapters) between sets., 18...
- Page 10 Tables Tables Relative Centrifugal Fields for the GH-3.8 Rotor. Entries in the table are calculated from the formula RCF = 1.12 r (RPM/1000)2and are then rounded to three significant digits., 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8...
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Page 11: Rotor
GH-3.8 Rotor This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. Specifications SPECIFICATIONS Maximum speed (buckets)..... . . 3750 rpm Maximum speed (Micro Plus carriers) . -
Page 12: Three Significant Digits
GH-3.8 Rotor Specifications Table 1 Relative Centrifugal Fields for the GH-3.8 Rotor. Entries in the table are calculated from the formula RCF = 1.12 r (RPM/1000) and are then rounded to three significant digits. Relative Centrifugal Field (× g) at r... -
Page 13: Description
Description Description Specific information about the GH-3.8 rotor is given here. Information about the use and care of centrifuges is contained in the individual instrument instruction manuals, which should be used in combination with this manual for complete centrifuge and rotor operation information. -
Page 14: Bottle Adapters
Table Bucket Covers Transparent covers made of a high-impact plastic are available for the GH-3.8 rotor buckets. Each cover requires an O-ring (made of ethylene propylene) that seats on a ledge inside the bucket. The covers are held in place by attached latches. Although the covers are not designed to contain aerosols... -
Page 15: Plate
GH-3.8 Rotor Description Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8 Rotor MODULAR DISK ADAPTERS (polypropylene) Max. Max. Adapter Part No. Nom. Nom. RCF at tube Tube Tubes tubes Adapter maximum Disks Color Vol. Dia. - Page 16 GH-3.8 Rotor Description Table 2 Modular Disk Adapters, Bottle Adapters, and Cups Available for the GH-3.8 Rotor (Continued) AEROSOLVE CANNISTER RACKS (polypropylene) Max. Adapter Part RCF at Nom. Nom. Max. No. Number Maximum Tube Tube Tubes Tubes Adapter Speed Color Vol.
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Page 17: Beckman Coulter Tubes And Bottles For The
Tube racks used with Aerosolve cannisters do not provide full tube support; some manufacturers’ plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks. Beckman Coulter highly recommends that you pretest other manufacturers’... - Page 18 GH-3.8 Rotor Description Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued) Adapter Volume Part Dimensions (mL) Description Number Set of Two Set of Four TUBES WITH SNAP-ON CAPS 359469 11 × 38 mm polypropylene 357448 354511...
- Page 19 GH-3.8 Rotor Description Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued) Adapter Volume Part Dimensions (mL) Description Number Set of Two Set of Four BIOVIALS 359470 14 × 55 mm polypropylene 566353 359149 344517 (each) BOTTLES...
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Page 20: Micro Plus Multiwell Plate Carriers
GH-3.8 Rotor Description Table 3 Beckman Coulter Tubes and Bottles for the GH-3.8 Rotor (Continued) Adapter Volume Part Dimensions (mL) Description Number Set of Two Set of Four BOTTLES (Continued) polycarbonate 96 × 130 mm — 358299 349846 (with screw cap polypropylene 96 ×... -
Page 21: Installation
GH-3.8 Rotor Installation Installation The Rotor Yoke NOTE Before the first use of a new rotor, lubricate the rotor pins and bucket pin sockets as described under Maintenance. Also, remove the lubrication decal over the rotor drive hole (in the yoke) and lubricate the inside of the drive hole as described under Maintenance. -
Page 22: Labware For Use With Micro Plus Carriers
GH-3.8 Rotor Installation Table 4 Labware for Use with Micro Plus Carriers Required Accessory Part Description Volume Numbers Description Part Number Rack Multiwell plate, 609844 — — — 300 μL/well 96-well, nonsterile (pkg/100) Cap strip, nonsterile 267002 (pkg/10) Deep-well polystyrene 267001 —... -
Page 23: Rotor Yoke And Centrifuge Drive Shaft. Be Sure The Pin In The Drive Shaft Is In The Groove In The Rotor
GH-3.8 Rotor Installation Figure 2 Rotor Yoke and Centrifuge Drive Shaft. Be sure the pin in the drive shaft is in the groove in the rotor yoke. Groove Drive Shaft Before using the tie-down nut, check it for damage such as distortion, splitting, or stripped threads;... -
Page 24: Buckets Or Carriers
GH-3.8 Rotor Installation Insert the torquing bar (356036) through the cross-drilled hole in the tie-down nut and turn the nut to the right (clockwise) until it is tight. Do not overtighten the tie-down nut. NOTE If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down nut is tight before each run. -
Page 25: Loading The Rotor
1. Capped tubes or bottles are designed to provide fluid containment. We strongly recommend that all containers carrying physiological fluids be capped to prevent leakage. 2. Bucket covers for the GH-3.8 rotor help to contain fluids within the bucket in the event of tube breakage or blood-bag failure. -
Page 26: Carriers. Contents Of Opposing Buckets Must Be The Same And Each Bucket Must Be Balanced On Its Pivotal Axis
GH-3.8 Rotor Loading the Rotor Figure 4 Examples of Correctly and Incorrectly Loaded Buckets and Carriers. Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal axis. BALANCED LOAD UNBALANCED LOAD Pivotal Axis... - Page 27 Placing empty blood bag cups or bottle adapters in the open buckets does not provide enough weight to prevent possible imbalance. Beckman Coulter supplies buckets in weight-matched sets to make balancing easier (the weight and warranty expiration date are marked on the side of each bucket). Modular disk adapters are also sold in weight-matched sets.
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Page 28: The J6 Have Curved Ends. Do Not Intermix Adapters (Or Disks From Adapters) Between Sets
Figure 5 The Bails (Vertical Supports) of the GH-3.8 (and GH-3.7) Modular Disk Adapters and the J6 Series Adapters are Not the Same. Bails for the GH-3.8 are straight and end in a disk; bails for the J6 have curved ends. -
Page 29: Using Buckets
GH-3.8 Rotor Loading the Rotor During a run, buckets and carriers swing 90 degrees from their at-rest position. The pivotal axis of a bucket or carrier can be imagined as a line extending across the bucket or carrier from one pivot pin to the other. -
Page 30: Assembling Modular Disk Adapters
GH-3.8 Rotor Loading the Rotor CAUTION Buckets are designed to be used only with modular disk adapters, bottle adapters, and blood bag cups. Do not pour samples directly into buckets, bottle sleeves, or blood bag cups. Do not load bottles or blood bags directly into the buckets. Do not use labware that is not specified for use in this rotor. -
Page 31: Loading Tubes In Adapters
GH-3.8 Rotor Loading the Rotor Loading Tubes in Adapters When placing tubes in modular disk adapters, it is important to make sure that they will not contact the rotor yoke during a run. • One way to do this is to place empty tubes in an adapter, place the adapter in a bucket on the rotor yoke, and manually swing the bucket to the horizontal position, making sure that all tubes clear the yoke. -
Page 32: Using Bottles In Adapters
GH-3.8 Rotor Loading the Rotor Using Bottles in Adapters Load bottles into their appropriate adapters (see Table 2), then place the filled adapters in the rotor buckets. If only two bottles are run, place them in opposite buckets. Make sure the other two buckets contain at least a minimal “blank”... -
Page 33: Bucket Covers
Bucket Covers Beckman Coulter recommends that you use bucket covers (360586) to help contain spills and glass particles in the event of tube breakage. Bucket covers can be used with all blood bags, bottles (except 230-mL conical), and tubes up to 130 mm long. They are not designed to contain aerosols. -
Page 34: Proper Placement Of O-Ring
GH-3.8 Rotor Loading the Rotor Install the covers as follows: Make sure that the bucket and the bucket cover surface are clean, dry, and undamaged. Make sure that the O-ring (961648) is in good condition and lightly coated with silicone vacuum... -
Page 35: Using Aerosolve Cannisters
Table 2 lists the Aerosolve tube racks and the number and sizes of tubes they accommodate. When Aerosolve cannisters are used in the GH-3.8 rotor buckets, pads (361269) must be placed beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation. -
Page 36: Using Micro Plus Multiwell Plate Carriers
GH-3.8 Rotor Loading the Rotor Using Micro Plus Multiwell Plate Carriers Micro Plus plate carriers (see Figure 12) can be used in place of the buckets normally used with the rotor. (For complete information about the carriers, see publication GS6-TB-011, which accompanies the carriers.) If only two carriers are run, they must be installed opposite each other... -
Page 37: Operation
Operation IF YOU USE MORE THAN ONE GH-3.8 ROTOR Complete sets of buckets, Micro Plus plate carriers, and labware may be used in any GH-3.8 rotor. Remember the following points: • Buckets are sold in weight-matched sets. Keep matched sets together and check their weights prior to use to avoid centrifuge imbalance. -
Page 38: Temperature Limits
Because the strength of glass and plastic tubes can vary between lots, and will depend on handling and usage, Beckman Coulter highly recommends that you pretest these tubes in the appropriate labware in the GH-3.8 rotor using water samples to determine optimal operating conditions. -
Page 39: Recommended G-Force Limits To Minimize Breakage Of Certain
GH-3.8 Rotor Operation Table 5 Recommended g-Force Limits to Minimize Breakage of Certain Glass Tubes and Bottles Made by Other Manufacturers in the GH-3.8 Rotor Safe g Safe Modular Volume Dimensions Force Speed Disk Adapter Tube Description (mL) (mm) (RCF) -
Page 40: Approximate Acceleration And Deceleration Times For The Gh
GH-3.8 Rotor Operation Table 6 Approximate Acceleration and Deceleration Times for the GH-3.8 Rotor in Allegra 6 Series, GS-6 Series, and Spinchron Series Centrifuges Acceleration Deceleration (H) Deceleration (L) (seconds) Instrument (seconds) (seconds) 60-Hz Nonrefrigerated Centrifuge 60-Hz Refrigerated Centrifuge 50-Hz Nonrefrigerated Centrifuge 50-Hz Refrigerated Centrifuge a. -
Page 41: Care And Maintenance
— frequently aluminum oxide—or heavy discoloration. If any of these signs are evident, do not run the rotor. Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center. -
Page 42: Cleaning
• Refer to publication TJ6-TB-011 for information on Aerosolve cannister maintenance. Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and tube materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. -
Page 43: Buckets
GH-3.8 Rotor Care and Maintenance Thoroughly rinse the cleaned rotor and components with water and air-dry upside down. Do not use acetone to dry the rotor. When the rotor is dry, lubricate the bucket sockets and pins as described under Maintenance. Before reinstalling the rotor, lightly lubricate the drive hole of the rotor with Anti-Seize (961660) to prevent the rotor from sticking, as follows: Apply the lubricant onto a swab. -
Page 44: Aerosolve Cannisters
RadCon Surface Spray or IsoClean Solution (for soaking), and Radiacwash Beckman Coulter does not however, warrant the performance of these products with respect to their effect on the carriers or their ability to decontaminate these parts. If the rotor or other components are contaminated with toxic or pathogenic materials, follow appropriate decontamination procedures as outlined by your laboratory safety officer. -
Page 45: Tube Breakage
GH-3.8 Rotor Decontamination While Beckman Coulter has tested these methods and found that they do not damage components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer. -
Page 46: Returning A Rotor
Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form, which may be obtained from your local Beckman Coulter sales office, should contain the following information: •... -
Page 47: Supply List
Contact Beckman Coulter Sales (1-800-742-2345 in the United States; worldwide offices are listed on the back cover of this manual) or see the Beckman Coulter Benchtop Rotors, Tubes & Accessories catalog (BR-9742, available at www.beckmancoulter.com) for detailed information on ordering rotors, tubes, and accessories. -
Page 48: Supplies
GH-3.8 Rotor Supply List Supplies Modular disk adapters (polypropylene)........see Table 2 Tubes and bottles . -
Page 49: Benchtop Rotor Warranty
Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions in effect at the time of sale, Beckman Coulter agrees to correct either by repair or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery... - Page 50 Benchtop Rotor Warranty Disclaimer Warranty-2 PN GS6-TB-003MD...
- Page 52 © 2018 Beckman Coulter, Inc. All Rights Reserved...
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