Beckman Coulter Allegra X-12 Series Instructions For Use Manual
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Beckman Coulter Allegra X-12 Series Instructions For Use Manual

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GX-IM-5AB
November 2013
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instruction For Use
Allegra X-12 Series
Centrifuges

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Summary of Contents for Beckman Coulter Allegra X-12 Series

  • Page 1 Instruction For Use Allegra X-12 Series Centrifuges GX-IM-5AB November 2013 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 Allegra X-12 Series Centrifuges GX-IM-5AB (November 2013) Copyright © 2013 Beckman Coulter, Inc. The following copyright statement is for position only. You may replace it with a statement approved by your group. All rights reserved. No part of this document may be...

  • Page 3: Safety Notice

    Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

  • Page 4: Electrical Safety

    Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge.

  • Page 5: Chemical And Biological Safety

    Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter. GX-IM-5AB...

  • Page 6: Rohs Notice

    Safety Notice RoHS Notice RoHS Notice These labels and materials declaration table (the Table of Hazardous Substance's Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. China RoHS Caution Label This label indicates that the electronic information product contains certain toxic or hazardous substances.

  • Page 7: Table Of Contents

    Contents Safety Notice, iii Alerts for Danger, Warning, Caution, and Note, iii Safety During Installation and/or Maintenance, iii Electrical Safety, iv Safety Against Risk of Fire, iv Mechanical Safety, iv Chemical and Biological Safety, v RoHS Notice, vi Introduction, xiii Certification, xiii Scope of Manual, xiii...
  • Page 8 Contents Name Rating Plate, 1-6 Specifications, 1-7 Available Rotors, 1-9 CHAPTER 2: Installation, 2-1 Introduction, 2-1 Space and Location Requirements, 2-1 Electrical Requirements, 2-2 Securing the Centrifuge to the Floor, 2-3 Test Run, 2-3 CHAPTER 3: Operation, 3-1 Introduction, 3-1 Summary of Run Procedures, 3-2 Manual...
  • Page 9 Contents Maintenance, 5-1 Cleaning, 5-2 Decontamination, 5-3 Sterilization and Disinfection, 5-3 Circuit Breaker and Fuses, 5-3 Storage and Transport, 5-4 Storage, 5-4 Supply List, 5-4 Replacement, 5-4 Supplies, 5-4 Beckman Coulter, Inc. Allegra X-12 Series Centrifuge Warranty...
  • Page 10 Illustrations Illustrations Centrifuge, 1-1 Control Panel, 1-4 Centrifuge Dimensions, 2-2 Door Latch Override, 4-6...
  • Page 11 Tables Tables Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed, 3-7 Diagnostic Message Chart, 4-2 Troubleshooting Chart, 4-5...
  • Page 12 Tables...

  • Page 13: Introduction

    Introduction Certification To ensure full system quality, Beckman Coulter Allegra X-12 series centrifuges are manufactured in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies.

  • Page 14: Typographic Conventions

    CFC-Free Centrifugation To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra X-12 series centrifuges. Radio Interference This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules.

  • Page 15: Recycling Label

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
  • Page 16 Introduction Recycling Label GX-IM-5AB...

  • Page 17: Description

    Figure 1.1) is a benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include: • Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor binding, and column centrifugations.

  • Page 18: Safety Features

    User messages alert the operator to conditions that may need attention. Safety Features Allegra X-12 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft). Safety features include the following.

  • Page 19: Rotor Chamber

    Description Controls and Indicators Rotor Chamber The aluminum rotor chamber is coated with epoxy enamel for corrosion resistance. The chamber is sealed by a foam gasket. Temperature Sensing and Control With the power on, the temperature control system is activated when the door is closed and locked. A sensor in the rotor chamber continuously monitors chamber temperature.
  • Page 20 Description Controls and Indicators Figure 1.2 Control Panel PULSE ENTER/ SAVE Digital Displays The displays provide run information, user messages, and diagnostics. • During a run, they show the actual (real-time) operating conditions. • When the run parameters are being entered, they show the set values selected. Set values can be recalled during operation by pressing .
  • Page 21 Description Controls and Indicators DECEL When the DECEL key is pressed, one of eleven available deceleration rates can be entered. Displays the selected deceleration rate throughout the run. PROGRAM • During setup—when the PROGRAM key is pressed, a program number (one of ten possible) can be selected and program parameters entered.

  • Page 22: Name Rating Plate

    Unlocks and opens the door. (The instrument will not accept the unlock command if the rotor is spinning.) Name Rating Plate A name rating plate is affixed to the rear of the instrument. Always mention the serial number and model number when contacting Beckman Coulter regarding your centrifuge. GX-IM-5AB...

  • Page 23: Specifications

    Description Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Specification Description Speed • Set speed — 200 to 10 200 rpm in 10-rpm increments • Speed display — actual rotor speed in 10-rpm increments or in RCF (when selected) Time •...
  • Page 24 Description Specifications Specification Description Finishes • Control panel — coated polystyrene copolymer • Housing surfaces — acrylic baking enamel Electrical requirements • 208-V, 60-Hz instrument — 187–229 VAC, 9 A, 60 Hz • 200-V, 50/60-Hz instrument — 180–220 VAC, 10 A, 50/60 Hz •...

  • Page 25: Available Rotors

    Description Available Rotors Available Rotors The following Beckman Coulter rotors can be used in the Allegra X-12 series centrifuge. The rotors are described in individual manuals that accompany each rotor. Number of Tubes ´ Rotor Rotor (´ g) at Nominal...
  • Page 26 Description Available Rotors 1-10 GX-IM-5AB...

  • Page 27: Chapter 2: Installation

    CHAPTER 2 Installation Introduction Preinstallation requirements have been sent prior to shipment of your centrifuge. WARNING This centrifuge weighs 121 kg (267 lb). Do not attempt to lift or move it without assistance. Space and Location Requirements WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids.

  • Page 28: Electrical Requirements

    NEMA 6-15R socket should be available. In regions where the instrument is supplied with an unterminated cord, a plug that meets local electrical and safety requirements must be supplied. (Contact your local Beckman Coulter Service Office for specific information regarding these requirements.)

  • Page 29: Securing The Centrifuge To The Floor

    Securing the Centrifuge to the Floor Allegra X-12 series centrifuges are certified to meet the requirements of the European CE mark. To meet these requirements, the centrifuge must be secured to the bench using the anchoring kit shipped with the instrument.
  • Page 30 Installation Test Run GX-IM-5AB...

  • Page 31: Chapter 3: Operation

    CHAPTER 3 Operation Introduction This section contains centrifuge operating procedures. A summary is provided at the start of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps.

  • Page 32: Summary Of Run Procedures

    Operation Summary of Run Procedures Summary of Run Procedures For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. Manual Run Turn the power switch on ( Press to open the chamber door; lift the door open. DOOR Install the rotor according to the applicable rotor manual, then close the chamber door.

  • Page 33: Programmed Run

    Operation Preparation Programmed Run Turn the power switch on ( Press to open the chamber door. DOOR Install the rotor according to the applicable rotor manual, then close the chamber door. Press , use the keypad or arrow keys to enter the required program number, then PROGRAM press ENTER/SAVE...

  • Page 34: Manual Operation

    When using multiwell-plate carriers, enter SX4750A as the rotor and limit top speed of the instrument to 3750 rpm in an Allegra X-12 series centrifuge or 4450 rpm in an Allegra X-15R (when running up to three stacked Beckman Coulter plates separated by cap strips; reduce speed to 2700 rpm if running four stacked plates).

  • Page 35: Entering Run Speed

    Operation Manual Operation Entering Run Speed Enter a run speed up to the maximum speed of the rotor in use. Or, enter a relative centrifugal field (RCF) value up to the maximum achievable RCF of the rotor. Press once to enter speed in RPM. RPM/RCF •...

  • Page 36: Entering Run Temperature (Allegra X-12R Only)

    Operation Manual Operation Press the next run-parameter key or ENTER/SAVE Entering Run Temperature (Allegra X-12R only) NOTE Temperature is factory set at 20°C on the Allegra X-12. Run temperature can be set from –10 to +40°C. If no value is entered, the centrifuge selects the last entered temperature.

  • Page 37: Starting A Run

    Operation Manual Operation Press the next run-parameter key or ENTER/SAVE Table 3.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to and from Maximum Speed SX4750 SX4750m SX4750A FX6100 (3 750 RPM) (3 750 RPM) (3 750 RPM) (10 200 RPM) Rate Accel Decel Accel...

  • Page 38: Pulse Function

    Operation Programmed Operation Remove the rotor (or containers). CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories. NOTE Depending upon the duration of the event, transient power-line interruptions may cause this equipment to decelerate or reset with the possible loss of in-process operation.

  • Page 39: Recalling A Program

    Operation Programmed Operation Enter run parameters (rotor, speed, time, temperature, acceleration/deceleration rate settings). a. Press ENTER/SAVE. • If you entered an unacceptable parameter, an error message will be displayed. b. (Press and enter an acceptable value, then press the next run-parameter key.) When all parameters are entered, press ENTER/SAVE.

  • Page 40: Disabling/Enabling The Audible Tones

    Operation Disabling/Enabling the Audible Tones After the rotor has stopped and the LED is lit, press DOOR • The door opens. Remove the rotor (or containers). Disabling/Enabling the Audible Tones The audible tones (beeps) that sound when a run-parameter key is pressed, or at the end of each run, or if an error occurs may be turned on and off by following the steps below (rotor must be at 0 rpm).

  • Page 41: Chapter 4: Troubleshooting

    Maintenance procedures are given in CHAPTER 5. For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; worldwide offices are listed on the inside back cover of this manual) for assistance. NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories, before requesting service by Beckman Coulter Field Service.
  • Page 42 DOOR LED comes on. Press CE to clear manual down with no brake message. If you close the door repeatedly and the problem continues, call Beckman Coulter Field Service. L7– Press down on the door, then press DOOR Door latches do not Door doesn’t open...
  • Page 43 Drive error, see with no brake then restart run. If problem repeats, call manual Beckman Coulter Field Service. Before trying to open the door, listen carefully and make sure that no sound is coming from the chamber (indicating a spinning rotor). Follow...

  • Page 44: Other Possible Problems

    Run shuts down Press CE to clear message, then restart run. If Imbalance error, see error with maximum problem persists, call Beckman Coulter Field manual brake Service. Other Possible Problems Operating problems that may not be indicated by diagnostic messages are described in Table 4.2,...

  • Page 45: Retrieving Your Sample In Case Of Power Failure

    Have a qualified service person measure line speed voltage while the instrument is operating. Electrical failure Make sure the power cord is securely connected; call Beckman Coulter Field Service. Motor failure Call Beckman Coulter Field Service. Door will not open Rotor spinning Wait until the rotor stops.

  • Page 46: Override

    Troubleshooting Retrieving Your Sample In Case of Power Failure Turn the power off and disconnect the power cord from the main power source. • No displays or LEDs are lit. Make sure that the rotor is not spinning. • No sound or vibration comes from the centrifuge. WARNING Never attempt to override the door interlock system while the rotor is spinning.

  • Page 47: Chapter 5: Care And Maintenance

    This section contains care and maintenance procedures that should be performed regularly. For maintenance not covered in this manual, contact Beckman Coulter Field Service (1-800-742-2345 in the United States; worldwide offices are listed on the inside back cover of this manual) for assistance. User...

  • Page 48: Cleaning

    Care and Maintenance Instrument Care Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to prevent chamber icing. If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use. a.

  • Page 49: Decontamination

    See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials. While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 50: Storage And Transport

    Storage and Transport Storage To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. Temperature and humidity conditions for storage should meet the environmental...

  • Page 51: Beckman Coulter, Inc

    (1) year after delivery of an Allegra X-12 Series centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
  • Page 52 Beckman Coulter, Inc. Allegra X-12 Series Centrifuge Warranty Warranty-2 GX-IM-5AB...
  • Page 53 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 54 © 2013 Beckman Coulter, Inc. All Rights Reserved...

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Allegra x-12 seriesAllegra x-15r

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