Sign In
Upload
Manuals
Brands
Beckman Coulter Manuals
Laboratory Equipment
PA 800 plus
Beckman Coulter PA 800 plus Manuals
Manuals and User Guides for Beckman Coulter PA 800 plus. We have
4
Beckman Coulter PA 800 plus manuals available for free PDF download: User Manual, Application Manual, Pre-Installation Manual
Beckman Coulter PA 800 plus User Manual (98 pages)
Pharmaceutical Analysis System System Maintenance
Brand:
Beckman Coulter
| Category:
Measuring Instruments
| Size: 2 MB
Table of Contents
Revision History
3
Safety Notices
5
Symbols and Labels
5
Table of Contents
11
Introduction
13
Install UV or PDA Detectors
14
Calibrate the PDA Detector
17
Install UV Detector Wavelength Filters
18
Integrated 488 Nm Solid-State Laser
24
Installation Requirements
24
Install the Solid-State Laser Module
24
Install the LIF Detector Filters
25
Install the Filter Housing into the Detector
26
Install the LIF Detector into the PA 800 Plus
27
Connect the Probe to the Clamp Bar
27
LIF Detector Calibration
29
Troubleshooting Procedures
32
Capillary Cartridge
33
Rebuild a Capillary Cartridge
33
Install a Capillary Cartridge into the PA 800 Plus
51
Universal Vials and Caps
54
Fill the Universal Vials
54
Fill the Micro Vials
55
Maintenance Procedures
57
Opening Levers, Electrodes, and the Interface Block
58
Clean Electrodes, Opening Levers, and the Interface Block
61
Coolant
62
Fiber Optics (UV and PDA Detectors)
64
Detectors)
65
Lamp
66
Instrument Care
73
LIF Care and Maintenance
73
Inspect the LIF
73
How to Clean the LIF
73
How to Store the LIF
73
Replace the Quad-Rings
74
Replace the Fuses
75
CHAPTER 3: Interfacing PA 800 Plus with Mass Spectrometry
77
CE-MS/MS Mode Cartridge Procedures
77
How to Install the Capillary in the Standard Cartridge
78
How to Install the Capillary in the EDA-Only Cartridge
79
Ground Connection
81
APPENDIX A: LIF Test MIX Calibration and Performance Tests
83
Relative Fluorescent Units (RFU
83
Calibration Correction Factor (CCF
83
Applying the CCF
84
Automatic Calibration
84
Calibration Method
85
Non-Fluorescent Solutions
86
LIF Calibration for Different Dimension Capillaries
86
LIF Calibration for Different Fluorescent Solutions
87
Standard System Performance Test
87
Troubleshooting
88
APPENDIX B: Evaluating PA 800 Plus UV Filters
91
Overview
91
Advertisement
Beckman Coulter PA 800 plus Application Manual (38 pages)
PA 800 plus Pharmaceutical Analysis System
Brand:
Beckman Coulter
| Category:
Measuring Instruments
| Size: 1 MB
Table of Contents
Revision History
3
Safety Notices
5
Symbols and Labels
5
Introduction
5
Caution, Biohazard Label
5
Caution, Moving Parts Label
6
High Voltage Electric Shock Risk Symbol
6
Sharp Object Label
6
Recycling Label
7
Disposal of Devices Containing Mercury Components
7
Restriction of Hazardous Substances (Rohs) Labels
8
Rohs Caution Label
8
Rohs Environmental Label
8
Alerts for Warning, Caution, Important, and Note
9
Table of Contents
11
Carbohydrate Labeling and Analysis Kit
13
Overview
13
Safety Information
13
General Precautions
14
Materials and Reagents
14
Contents of this Kit
14
Materials Needed but Not Provided in Kit
14
Storage Conditions
15
Overview of the Procedure
15
Releasing Oligosaccharides from Glycoproteins
15
Enzymatic Release of the N-Linked Oligosaccharides
16
Working with the Supernatant
16
Principle of the Labeling Method
16
Preparing the Labeling Reagents
17
Choosing the Proper Fluorophore
17
Preparing the Reagents
17
Preparing the Standards
18
Performing the Labeling Reaction
18
Preparing the PA 800 Instrument
20
Installing and Pre-Conditioning the N-CHO Capillary
21
Clean the Interface Block
22
Insert the Cartridge
22
Preparing the Buffer Trays
22
Sample Vial Setup
24
Running the Samples
25
Initial Conditions
25
LIF Detector Initial Conditions
26
Time Program
27
Evaluation of Results
29
System Shutdown and Capillary Storage
30
Short Term Storage (<24 Hours) of Capillary
30
Long Term Storage (>24 Hours) of Capillary
30
Troubleshooting Procedures
31
Appendix
32
LIF Detector Calibration Procedure
32
Setting the Calibration Corrector Factors (Ccfs)
32
Automatic Calibration
33
CCF Troubleshooting Procedures
36
Ccf <1
36
No Step Change Detected
36
Beckman Coulter PA 800 plus Application Manual (34 pages)
Pharmaceutical Analysis System, SDS-MW Analysis
Brand:
Beckman Coulter
| Category:
Laboratory Equipment
| Size: 1 MB
Table of Contents
Revision History
3
Safety Notices
5
Symbols and Labels
5
Table of Contents
11
CHAPTER 1 Introduction
13
Protein Size Standard
13
Internal Standard
13
Intended Use
14
Materials and Reagents
14
Storing the Assay Components
15
Reagents
15
Materials Required but Not Provided by Beckman Coulter
15
Beckman Coulter Consumables
15
Preparing the PA 800 Plus Instrument
16
Installing the Capillary
16
Cleaning the Interface
16
Inserting the Cartridge/Calibrating the PDA
17
Sample Preparation
17
Preparation of SDS-MW Size Standard
17
Preparation of Protein Sample
18
Desalting the Protein Sample
18
Protein Sample Concentration
18
Reducing the Protein Sample
18
Non-Reduced Protein Sample Preparation
19
Preparing Alkylation Reagent
19
Preparing the Sample
19
Sample Vial Setup
20
Buffer Vial Preparation and Loading
21
Preparing the Reagent Vials
21
Tray Configuration
23
Inlet Buffer Tray
23
Outlet Buffer Tray
24
Running the Assay
24
Launching the SDS MW Instrument
24
Conditioning a New Capillary
25
Running SDS-MW Size Standard and Test Samples
26
Checking the Separation Results
28
Estimating Protein Molecular Weights
28
Troubleshooting Guide
29
Sample
30
CHAPTER 2: Instrument and Detector Initial Conditions
31
Instrument Initial Conditions (All Methods)
31
Detector Initial Conditions (All Methods)
32
Capillary Conditioning Method (SDS MW Conditioning - PA 800 Plus.met)
32
SDS-MW Separation Method
33
Shutdown Method
33
Advertisement
Beckman Coulter PA 800 plus Pre-Installation Manual (28 pages)
Pharmaceutical Analysis System. 32 Karat Software Version 9.1 and PA 800 plus Software Version 1.1
Brand:
Beckman Coulter
| Category:
Laboratory Equipment
| Size: 0 MB
Advertisement
Related Products
Beckman Coulter ProteomeLab XL-A
Beckman Coulter ProteomeLab XL-L
Beckman Coulter P/ACE MDQ
Beckman Coulter 45 Ti Rotor
Beckman Coulter Airfuge
Beckman Coulter Allegra 21 Series
Beckman Coulter Allegra 21R
Beckman Coulter Allegra 25R
Beckman Coulter Allegra 6 Series
Beckman Coulter Allegra 64R
Beckman Coulter Categories
Laboratory Equipment
Measuring Instruments
Cash Counter
Medical Equipment
Desktop
More Beckman Coulter Manuals
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL