Related Manuals for Beckman Coulter JA-14.50
Summary of Contents for Beckman Coulter JA-14.50
- Page 1 Instructions For Use JA-14.50 Fixed-Angle Rotor For Use in Beckman Coulter Avanti J Series PN B32164AC October 2016 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 PN B32164AC (October 2016) © 2016 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
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Page 3: Revision History
Issue AC, 07/2016 — Updates were made to the following sections: Safety Information for the JA-14.50 Rotor, Specifications, Description, Preparation and Use, Tubes, Replacement Rotor Parts, Supplies. - Page 4 Revision History PN B32164AC...
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Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to use this equipment. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative. - Page 6 This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.
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Page 7: Table Of Contents
Dual-Locking Lid, 10 Single-Locking Lid, 11 If the Rotor Sticks to the Drive Hub, 12 Tubes, 13 Tubes for JA-14.50 Rotors Manufactured before October 2016, 13 Tubes for JA-14.50 Rotors Manufactured after October 2016, 16 Temperature Limits, 17 Times, 18... - Page 8 Contents Beckman Coulter, Inc. J Series Rotor Warranty viii...
- Page 9 Installing the Rotor, 8 Using the Rotor Removal Tool, 12 Rotor Serial Number with T, 13 Rotor Lid Serial Number with T, 13 Examples of Tubes with Minimum and Moderate Crazing, 18 Relative Centrifugal Fields Chart for the JA-14.50 Rotor, 21...
- Page 10 Adapters to support tubes from other manufacturers for the JA- 14.50 Rotor manufactured before October 2016, 13 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotor manufactured prior to October 2016, 14 Adapters to support tubes from other manufacturers for the JA- 14.50 Rotor manufactured after October...
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Page 11: Fixed-Angle Rotor
JA-14.50 Fixed-Angle Rotor Specifications Maximum speed ........14,000 RPM Critical speed range . -
Page 12: Description
Beckman Coulter JA-14.50 rotors are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges. The JA-14.50 fixed-angle rotor is designed to hold up to sixteen 50-mL tubes at a 35-degree angle (see Figure 1). - Page 13 JA-14.50 rotors manufactured after October 2016 have a slightly tapered cell profile, which improves performance of most commercially available 50 mL conical tubes. However, some tubes and adapters that were compatible prior to October 2016 are no longer compatible.
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Page 14: Preparation And Use
Preparation and Use Preparation and Use Specific information about the JA-14.50 rotor is given here. Use the Rotors and Tubes for Beckman Coulter J Series Centrifuges manual (publication JR-IM-10) and the centrifuge instruction manual along with this manual for complete rotor and accessory operation information. - Page 15 JA-14.50 Fixed-Angle Rotor Preparation and Use Figure 3 Typical Examples of Arranging Sixteen or Fewer Tubes in the Rotor PN B32164AC...
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Page 16: Operation
(Figure The rotor pins are positioned parallel to the Beckman Coulter name engraved at the center of the rotor body (see Figure 6). - Page 17 JA-14.50 Fixed-Angle Rotor Preparation and Use Figure 4 Rotor Drive Pin Location and Orientation 1. Rotor Drive Hole 3. Avanti J Centrifuge Drive Spindle Hub 2. Drive Pins Figure 5 Centrifuge Drive Hub Configurations 1. Drive Hub Grooves (4) 6. Teeth (2) 2.
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Page 18: Rotor Lid Configurations
JA-14.50 Fixed-Angle Rotor Preparation and Use Figure 6 Installing the Rotor 1. Top View 2. Side View Rotor Lid Configurations The rotor has two available lid configurations. • The dual-locking lid (B19947) allows the lid to be attached to and removed from the rotor outside the centrifuge, so that tubes can be loaded and unloaded under a safety hood. -
Page 19: Rotor Installation
(clockwise) until secure. Do not overtighten. Turn the rotor until the Beckman Coulter name or the engraved drive pin alignment indicators are either horizontal or perpendicular to the drive hub pins or teeth (see Figure Or, rotate the drive spindle to align the hub pins or teeth with the rotor drive pins. -
Page 20: Run Procedure
JA-14.50 Fixed-Angle Rotor Preparation and Use Secure the rotor to the centrifuge drive spindle as follows: a. Dual-locking lid: press the lid knob down and turn it to the right (clockwise) until secure, no more than two full turns. Do not overtighten. -
Page 21: Single-Locking Lid
JA-14.50 Fixed-Angle Rotor Preparation and Use Grasp the round lid knob with your other hand and turn the knob to the left (counterclockwise) until the stem disengages from the drive hub threads. Hold the knob or place your hands on the rotor and lift the rotor straight up off the drive hub. -
Page 22: If The Rotor Sticks To The Drive Hub
JA-14.50 Fixed-Angle Rotor Preparation and Use If the Rotor Sticks to the Drive Hub If the rotor sticks to the centrifuge drive hub, use the removal tool (360998) to release it as follows (see Figure Figure 7 Using the Rotor Removal Tool 1. -
Page 23: Tubes
(Maximum fill volume is the maximum amount that can be centrifuged in the container listed.) NOTE JA-14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number (Figure 8 Figure... -
Page 24: Available Beckman Coulter Bottles And Tubes For The
JA-14.50 Fixed-Angle Rotor Preparation and Use Table 1 Adapters to support tubes from other manufacturers for the JA-14.50 Rotor manufactured before October 2016 Effective Effective Type and Speed/RCF/ Volume Tube Description Required Adapter (mm) (mm) k Factor conical Polypropylene conical tube B40158 125.1... -
Page 25: Available Beckman Coulter Bottles And Tubes For The
JA-14.50 Fixed-Angle Rotor Preparation and Use Table 2 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotor manufactured prior to October Nominal Tube Description Part Nominal Required Tubes Dimensions Number Volume Adapter Speed/RCF/ and Volume (mL) Adapter k Factor 29 ... -
Page 26: Tubes For Ja-14.50 Rotors Manufactured After October 2016
JA-14.50 Fixed-Angle Rotor Preparation and Use Tubes for JA-14.50 Rotors Manufactured after October 2016 NOTE JA-14.50 rotors and lids manufactured after October 2016 include the letter “T” after the serial number (see Figure 8 Figure CAUTION The tubes listed in Table 3 should be used one time only. Tube damage and sample leakage will result from repeated use. -
Page 27: Temperature Limits
JA-14.50 Fixed-Angle Rotor Preparation and Use Table 4 Available Beckman Coulter Bottles and Tubes for the JA-14.50 Rotor manufactured after October 2016 Nominal Nominal Required Tubes Dimensions Part Volume Adapter Speed/RCF/ and Volume Tube Description Number (mL) Adapter k Factor 18 ... -
Page 28: Run Times
Figure 10 Examples of Tubes with Minimum and Moderate Crazing Run Times The k factor of the rotor is a measure of the rotor’s pelleting efficiency. Beckman Coulter has calculated the k factors for all of its rotors, at maximum speed with full tubes, using the following formula: ⁄... - Page 29 JA-14.50 Fixed-Angle Rotor Preparation and Use Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows: EQ 5 14, 000 ---------------------------------------- - rated actual run speed Run times can also be estimated from data established in prior experiments using a different rotor if the k factor of the previous rotor is known.
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Page 30: Run Speeds
EQ 7 1.2 g/mL reduced maximum speed = (14,000 RPM) ---------------------------------------------------------- density of tube contents Table 5 Relative Centrifugal Fields for the JA-14.50 Rotor Rotor Speed (RPM) Relative Centrifugal Field (g) At r (160 mm) At r... -
Page 31: Care And Maintenance
JA-14.50 Fixed-Angle Rotor Care and Maintenance Figure 11 Relative Centrifugal Fields Chart for the JA-14.50 Rotor Care and Maintenance Maintenance 1. Daisy Knob 2. Small O-ring (961696) 3. Rotor Body 4. Check for Corrosion 5. Large O-ring (885501) 6. Dual Locking Lid (B19947) NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface. -
Page 32: Cleaning
Wash the rotor and lid in a mild detergent, such as Solution 555 (339555), that won’t damage the rotor. Dilute the detergent with water (10 parts water to 1 part detergent). * Call 1-800-742-2345 (U.S.A. or Canada); outside the U.S, contact your local Beckman Coulter office or visit us on the web at www.beckmancoulter.com. PN B32164AC... -
Page 33: Decontamination
JA-14.50 Fixed-Angle Rotor Care and Maintenance The Rotor Cleaning Kit (339558) contains two plastic-coated brushes and two quarts of Solution 555 for use with rotors and accessories. NOTE Do not wash rotor components in a dishwasher. Do not soak in detergent solution for long periods, such as overnight. -
Page 34: Sterilization And Disinfection
Ethanol is a flammability hazard. Do not use it in or near operating centrifuges. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 35: Returning A Rotor
Returning a Rotor Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. A return authorization form may be obtained from your local Beckman Coulter sales office. The form should contain the following information: •... -
Page 36: Supplies
JA-14.50 Fixed-Angle Rotor Supply List Supplies NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com. Description Part Number Tubes and adapters Table Table Table 3, and Table 4 Rotor removal tool 360998 Rotor Cleaning Kit 339558 Silicone vacuum grease (1 oz) - Page 37 A defective rotor will be replaced by Beckman Coulter, Inc. at its then current list price less a credit based upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or...
- Page 38 Beckman Coulter, Inc. J Series Rotor Warranty Warranty-2 PN B32164AC...
- Page 40 Related Documents Rotors and Tubes for Beckman Coulter J2, Additional References J6,and Avanti J Series Centrifuges • Chemical Resistances for Beckman Coulter PN JR-IM-10 Centrifugation Products (IN-175) • Rotors • Beckman Coulter High Performance, High • Tubes, Bottles, and Accessories Speed, High Capacity Rotors, Tubes &...