Related Manuals for Beckman Coulter Optima L-XP
Summary of Contents for Beckman Coulter Optima L-XP
- Page 1 Instructions For Use Optima L-XP Series Preparative Ultracentrifuges PN LXP-IM-7AB October 2013 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
- Page 2 Beckman Coulter, Inc. Beckman Coulter, the stylized logo, and Quick-Seal are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
- Page 3 This instrument is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field Service representative must reinstall and relevel the instrument in its new location.
- Page 4 Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand.
- Page 5 Do not lean on the instrument or place items on it while it is operating. It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service. PN LXP-IM-7AB...
- Page 6 Safety Notice Chemical and Biological Safety PN LXP-IM-7AB...
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Page 7: Table Of Contents
Contents Safety Notice, iii Alerts for Warning, Caution, Important, and Note, iii Safety During Installation and/or Maintenance, iii Electrical Safety, iv Safety Against Risk of Fire, iv Mechanical Safety, iv Chemical and Biological Safety, iv Introduction, xv Certification, xv Scope of Manual, xv CFC-Free Centrifugation, xvi... - Page 8 Contents Name Rating Plate, 1-4 Controls and Indicators, 1-4 Power, 1-4 Key Switch Positions, 1-5 Control Panel, 1-5 Specifications, 1-9 Available Rotors, 1-10 CHAPTER 2: Operation, 2-1 Introduction, 2-1 Power, 2-1 Key Switch Positions, 2-1 Normal Position, 2-2 Locked Position, 2-2 Zonal Position, 2-2 Touchscreen...
- Page 9 Contents Speed Reduction for Precipitating Solutions, 2-30 Sedimentation Coefficient from Run Data, 2-31 Sedimentation Coefficient From Molecular Mass, 2-32 Restore Windows, 2-33 CHAPTER 3: Simulations, 3-1 Using Simulation Features, 3-1 Pelleting, 3-1 ESP Rate Zonal, 3-3 ESP Plasmid Run, 3-4 ESP RNA (BEST), 3-6 ESP RNA...
- Page 10 Information, B-2 GNU General Public License, Version 2, June 1991, B-3 Preamble, B-3 GNU General Public License Terms and Conditions for Copying, Distribution and Modification, B-4 Beckman Coulter, Inc. Optima L, LE, L-XP, and XL Preparative Ultracentrifuges Warranty Related Documents...
- Page 11 Illustrations Illustrations Optima L-XP Control Head, 1-5 Key Switch Positions, 1-5 Control Panel, 1-6 Settings Screen, 1-6 Step Program Screen, 1-7 Key Switch Positions, 2-1 Touchscreen and Keypad Operation, 2-3 User Login Screen, 2-5 System Management Screen, 2-6 Add User...
- Page 12 Screen, 4-4 Tube Catalog, 4-5 Selected Tube Specifications, 4-6 Chemicals/UPAC Name Screen, 4-7 Chemical Resistances Screen, 4-8 4.10 Optima L-XP Control Head with USB Hub, 4-9 4.11 Export Data Screen, 4-9 4.12 Logs Menu, 4-10 4.13 Record, 4-11 Removing the Control Head...
- Page 13 Tables Tables Step Program Screen Features, 1-7 Acceleration and Deceleration Profiles, 2-14 Diagnostic Message Chart, 5-2 Required Wire Connections, A-3 xiii...
- Page 14 Tables...
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Page 15: Safety Notice
Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com. Scope of Manual This manual is designed to familiarize you with the Optima L-XP series ultracentrifuge, its functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommendsthat you read this entire manual, especially the... -
Page 16: Cfc-Free Centrifugation
For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device. -
Page 17: Software Copyright
The date code on the label or product indicates the date of manufacture. Software Copyright The Optima L-XP eXPert operating software (a Microsoft Windows-based computer program) is protected by international copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws that may result in civil or criminal penalties. This computer program is... - Page 18 Introduction Software Copyright xviii PN LXP-IM-7AB...
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Page 19: Description
The Optima L-XP ultracentrifuge is used to generate centrifugal forces for the separation of particles. The Optima L-100 XP has a maximum rotational speed of 100,000 RPM; the L-80 XP runs up to 80,000 RPM. Classified S, it can be used with all currently manufactured Beckman Coulter preparative rotors. -
Page 20: Safety Features
Requirements for printer availability and hookup procedures. Safety Features The Optima L-XP ultracentrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Door The high-strength structural steel chamber door has a solenoid interlock to prevent operator contact with a spinning rotor. -
Page 21: Dynamic Rotor Inertia Check (Dric)
Vacuum System The Optima L-XP uses a diffusion pump in series with a mechanical vacuum pump to reduce chamber pressure to below 5 microns (0.7 Pa). The system is automatically activated when... -
Page 22: Temperature Sensing And Control
(If the radiometer fails, a diagnostic message appears and the system reverts to control by the thermistor; the run will continue.) The Optima L-XP uses a solid state thermoelectric refrigeration and heating system. Neither coolant nor water is needed; cooling is provided by forced air from the fans. -
Page 23: Key Switch Positions
Description Controls and Indicators Figure 1.1 Optima L-XP Control Head BkSp VACUUM STOP POWER OFF Key Switch Positions A key interlock switch is used to select normal, locked, or zonal operation (see Figure 1.2). Use the normal position for routine closed-door centrifugation, the locked position to prevent settings being changed during the run, and the zonal position for runs in which a zonal rotor is loaded and unloaded while spinning. -
Page 24: Control Panel
Description Controls and Indicators Figure 1.3 Control Panel Optima L-XP Ultracentrifuge Speed (RPM): 0 Time(hr:min): 0:00 Temp( C): 24.6 File Setup Simulate Calculate Reference Logs Window Optima L-XP Help BECKMAN Stopped COULTER. Optima ™ L-XP EXPERT SOFTWARE Speed (RPM) Time... -
Page 25: Step Program Screen
Description Rotor Menu Rotor in use is entered or selected from the pull-down menu Rotor List Displays a full list of Beckman Coulter rotors Tube List Displays a list of tubes available for the selected rotor Tube Menu Tube in use is entered (optional) - Page 26 Description Controls and Indicators Table 1.1 Step Program Screen Features Screen Element Description Acceleration First step only—Maximum acceleration or one of nine slower acceleration profiles can be entered to minimize sample-to-gradient interface disturbance; Max (maximum) is the default Deceleration Last step only—Maximum deceleration, one of nine slower deceleration profiles, or No Brake can be entered to minimize sample-to-gradient interface disturbance;...
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Page 27: Specifications
Description Specifications Specifications NOTE Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Specification Description Speed • Optima L-100 XP set speed: — 1000 to 100,000 RPM in 100-RPM increments • Optima L-80 XP set speed: —... -
Page 28: Available Rotors
Available Rotors All currently manufactured Beckman Coulter ultracentrifuge rotors can be used in the Optima L-XP series ultracentrifuges. The rotors are described in individual manuals that accompany each rotor. Information on rotors and accessories is also available in Rotors and Tubes for Preparative Ultracentrifuges (LR-IM) and in the Beckman Coulter Ultracentrifuge Rotors, Tubes &... -
Page 29: Chapter 2: Operation
CHAPTER 2 Operation Introduction This chapter contains manual and programmed operating procedures. Refer to the applicable rotor manual for information about the use and care of rotors and accessories. Power If the power is off, flip the circuit breaker at the right side of the instrument to on (up position). Power should be left on except in the case of an emergency or when maintenance is required. -
Page 30: Normal Position
Zonal Key Switch Position Touchscreen Interface The operation of the Optima L-XP is controlled through the touchscreen interface display, which comprises touch-sensitive display fields and buttons for entering and displaying run parameters PN LXP-IM-7AB... -
Page 31: Basic Operation
Figure 2.2. Figure 2.2 Touchscreen and Keypad Operation System Optima L-XP Ultracentrifuge Speed (RPM): 0 Time(hr:min): 0:00 Temp( C): 24.6 File Setup Simulate Calculate Reference Logs Window Optima L-XP Help Menu BECKMAN Stopped COULTER. Optima ™ L-XP EXPERT SOFTWARE Numeric... -
Page 32: Logging In
Operation Logging In • Optima L-XP—Field Service and System Management access, Change PIN and Export Data functions, and About Optima L-XP which contains software version number, copyright information, and local field service name and contact information Keypad—Information (except system menus and the... -
Page 33: System-Level Operations
To log in as the Administrator: Log in as the Administrator (if you are not logged in already). On the Settings screen, select from the Optima L-XP drop-down menu. The System Management System Management screen appears as shown in Figure 2.4. -
Page 34: Adding A User
Operation System-Level Operations Figure 2.4 System Management Screen Adding a User To add a user: From the System Management screen, press the button. The Add User Name screen Add User appears as shown in Figure 2.5. Figure 2.5 Add User Screen Enter a user name on the keypad. -
Page 35: New Pin Screen
Operation System-Level Operations When the user name is entered, press the button. The New PIN screen appears as shown Figure 2.6. Figure 2.6 New PIN Screen Enter a PIN number 4 to 6 digits in length on the keypad, and press the button to accept. -
Page 36: Copying Users
Operation System-Level Operations Enter the PIN number again, and press the button to accept. The Set Role screen appears as shown in Figure 2.8. On this screen, Administrators must assign one of three security roles that control access to specific instrument functions: •... -
Page 37: Set Pin Number
Operation System-Level Operations To copy a user: From the System Management screen, select a user name from the user list and press the button. The Copy User Name screen appears. Copy User Enter a user name on the keypad. Uppercase letters are default. To enter lowercase letters, press the key. -
Page 38: Set Role
Operation System-Level Operations Set Role The security level assigned to existing users may be changed if access to specific instrument functions and programs needs to be expanded or restricted. To assign a role to a user: From the System Management screen, select a user name from the user list and press the button. -
Page 39: Require User Login
Operation System-Level Operations Require User Login A user may be required to log in prior to starting a run. With each login, the user actions are recorded in the run log. To set up required user login: From the System Management screen, check the box next to Require User Login (see Figure 2.9) to enable this function. -
Page 40: Enable Run Comment
Operation Manual Operation Enable Run Comment When a Run Comment is enabled, the user can enter a run comment (up to 255 characters) before starting a run. A run comment is typically used to record the batch and lot information. To enable run comment: From the System Management screen, check the box next to Enable Run Comment to enable this function. - Page 41 Operation Manual Operation To set up a run manually: Enter required speed (between 1000 RPM and the rotor maximum speed) by keying in the numbers or selecting a speed from the Set Speed (RPM) drop-down menu. Enter required time (up to 999 hours and 59 minutes) by keying in the hours and minutes or selecting a time (or Hold) from the Time (hr:min) drop-down menu.
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Page 42: Delay Start Screen
Operation Manual Operation Table 2.1 Acceleration and Deceleration Profiles Acceleration Deceleration Transition Transition Profile Speed Profile Speed Number (RPM) Time Number (RPM) Time Maximum Acceleration Maximum Deceleration 2:00 2:00 2:40 2:40 3:00 3:00 3:00 3:00 4:00 4:00 4:30 4:30 4:00 4:00 5:20 5:20... -
Page 43: Programmed Operation
Operation Programmed Operation To cancel Delay Start, press the button on the control panel. Stop Press , then enter chamber precool or preheat temperature (see Figure 2.12). Precool/Heat Press to begin temperature control. Start Temp Figure 2.12 Precool/Heat Temperature Screen NOTE To save the run parameters for future recall, press Save... -
Page 44: Setup Menu
Operation Programmed Operation Figure 2.13 Setup Menu If you downloaded a program before you saved it, and you are still in the Settings window, you can go back to that program window by pressing . If you downloaded a program before you View Program saved it, and you are no longer in the Settings window, you may be able to retrieve the program from the File menu (see... -
Page 45: Step Program
Operation Programmed Operation Step Program To use step program: Select from the Setup menu (see Figure 2.15). Step Program Run Figure 2.15 Step Program Screen Enter rotor name and tube type (optional) or press (rotor) and select from the drop-down R... -
Page 46: Save As Dialog
Operation Programmed Operation To save the program for later recall, press . The Save As dialog box opens, and you can Save use the on-screen keyboard (see Figure 2.16) to specify a filename. Figure 2.16 Save As Dialog NOTE If you do not save the program under a new filename, it will be overwritten when a new step program is run. -
Page 47: Rpm To Rcf Toggle
Operation Programmed Operation RPM to RCF Toggle To toggle between RPM and RCF: On the Settings screen, press the button to toggle between RPM and RCF (relative Display RCF centrifugal field) modes. RPM indicates rotor speed in revolutions per minute while RCF represents the ratio of the centrifugal acceleration at a specified radius and speed to the standard acceleration of gravity. - Page 48 Operation Programmed Operation To use RCF: Select from the Setup menu. RCF Run Enter rotor and tube in use or press (rotor) and select a rotor and tube combination from R... the rotor library. NOTE Continuous flow and zonal rotors are only used in single step runs; no conversion functions apply to these rotors.
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Page 49: W2T,
Operation Programmed Operation Figure 2.18 w t Run Screen To set the run to stop at w Select from the Setup menu. w2t Run Enter rotor and tube in use or press (rotor) and select a rotor and tube combination from R... -
Page 50: Rotor Conversion
Operation Programmed Operation Rotor Conversion Calculates time required to duplicate run conditions using a different rotor and tube combination. To enter a substitute rotor speed: Select from the Setup menu. Substitute Rotor Run In the First Rotor Information area (see Figure 2.19), enter the rotor and tube used in the original protocol. -
Page 51: Auto Pelleting
Operation Programmed Operation Press Calculate The run time will be calculated and displayed. You can enter the additional run parameters then download the settings to the Settings window and start the run, or save as a program before downloading. NOTE To save the program for future recall, press Save , then enter a program name in the dialog... -
Page 52: Auto Rate Zonal
Operation Programmed Operation Figure 2.20 Auto Pelleting Run Screen Press to transfer the run parameters to the Settings window. If not saved to a Download specific filename, the program is automatically saved to a temporary file called LastAutoPelleting.apr. Press , then to run the program. -
Page 53: Auto Rate Zonal Screen
Operation Programmed Operation Figure 2.21 Auto Rate Zonal Screen Select the gradient type. Select particle density (in g/mL). The run time required will be calculated and displayed. You can enter the additional run parameters then download the settings to the Settings window and start the run, or save as a program before downloading. -
Page 54: Recall Program
Press , then to run the program. Enter Start Remote Operation The Optima L-XP ultracentrifuge may be operated from a remote computer. See APPENDIX B, Remote Access, for more information on this feature. 2-26 PN LXP-IM-7AB... -
Page 55: Using Calculation Features
Operation Using Calculation Features Using Calculation Features The calculations feature performs a variety of calculations commonly used in ultracentrifugation. These calculations help simplify run preparation and are accessed from the Calculate menu (see Figure 2.23) during the setup process, but cannot be downloaded to a setup screen. Figure 2.23 Calculate Menu Pelleting Time Calculation To calculate the pelleting time:... -
Page 56: Concentration Measures
Operation Using Calculation Features Enter the speed in RPM or RCF. Press Calculate The time required to pellet the particles appears in the Pelleting Time (hr:min) field, and the RCF will be displayed if you selected RPM, or RPM will be displayed if you selected RCF. Note the calculated time, then press to close the window. -
Page 57: Refractive Index/Density
Operation Using Calculation Features Note the calculated parameters, then press to close the window. Close Refractive Index/Density To calculate values of refractive index, density, or concentration: Select from the Calculate menu. Refractive Index/Density Enter the value for one of: refractive index, density, or molarity (see Figure 2.26). -
Page 58: Speed Reduction For Precipitating Solutions
Operation Using Calculation Features To calculate reduced speed for dense solutions: Select from the Calculate menu. Reduced Speed for Dense Solutions Select a rotor and tube combination (see Figure 2.27). The maximum speed for that rotor and tube combination will be displayed. Figure 2.27 Calculate Reduced Rotor Speed for Dense Solutions Screen Enter the average density of the tube contents in g/mL. -
Page 59: Sedimentation Coefficient From Run Data
Operation Using Calculation Features To calculate reduced speed for precipitating solutions: Select from the Calculate menu. Reduced Speed for Precipitating Solutions Select a rotor and tube combination (see Figure 2.8). Figure 2.28 Calculate Reduced Rotor Speed for Precipitating Solutions Screen Enter the average density of the tube contents in g/mL. -
Page 60: Sedimentation Coefficient From Molecular Mass
Operation Using Calculation Features To calculate sedimentation coefficient: Select from the Calculate menu. Sedimentation Coefficient from Run Data Select a rotor and tube combination, location from meniscus, gradient, particle density speed, time, and run temperature from the drop-down menus (see Figure 2.29). -
Page 61: Restore Windows
Operation Restore Windows Enter the molecular mass or molecular length of the molecule (see Figure 2.30). Figure 2.30 Estimate Sedimentation Coefficient from Molecular Mass Screen Press Calculate The sedimentation coefficient is displayed. Note the calculated s, then press to close the window. Close Restore Windows If multiple windows are open (such as calculations, simulations, logs, rotor or tube lists), you can... - Page 62 Operation Restore Windows 2-34 PN LXP-IM-7AB...
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Page 63: Simulations
CHAPTER 3 Simulations Using Simulation Features When running a separation using CsCl, it is critical to keep the salt from precipitating out and potentially causing a rotor mishap. This is accomplished by running the rotor below the speed at which the salt precipitates. ESP (Efficient Sedimentation Program) was developed as a way of predicting optimum run conditions for pelleting RNA over a wide variety of rotor and tube combinations. -
Page 64: Esp Pelleting Screen
Simulations ESP Pelleting separation of sample components to determine the shortest possible run duration that precludes precipitation. The simulation predicts the time at which the required separation will be achieved. If a step gradient instead of a linear gradient is used, the sample will migrate faster toward the tube bottom. -
Page 65: Esp Rate Zonal
Simulations ESP Rate Zonal Press to transfer the run parameters to the Settings window. If not saved to a Download specific filename, the program is automatically saved to a temporary file called LastESPPelleting.spr. Press , then to run the program. Enter Start ESP Rate Zonal... -
Page 66: Esp Plasmid Run
Simulations ESP Plasmid Run Select the rotor (swinging bucket only) and tube in use. Select the gradient type. Select the particle density. Enter the run temperature. Press Simulate Protocol The simulation positions the particle with the largest s value (fastest pelleting) at the bottom of the scale, and shows the relative positions of other selected particles along the tube length. -
Page 67: Esp Plasmid Run Screen
Simulations ESP Plasmid Run Select the plasmid size (see Figure 3.4). Figure 3.4 ESP Plasmid Run Screen Select the rotor and tube in use from the drop-down menu. Press Simulate Protocol The simulation is displayed on the graph. • The green and blue curves represent the relative concentration of linear and supercoiled DNA, respectively. -
Page 68: Esp Rna (Best)
Simulations ESP RNA (BEST) Press to transfer the run parameters to the Settings window. If not saved to a Download specific filename, the program is automatically saved to a temporary file called LastPlasmid.pla. Press , then to run the program. Enter Start ESP RNA (BEST) -
Page 69: Esp Rna (Fast)
Simulations ESP RNA (Fast) Press Simulate Protocol The simulation is displayed on the graph. • The green and blue curves represent the relative concentration of linear and supercoiled DNA, respectively. • The red curve represents the density of the CsCl gradient with respect to the right-hand scale. -
Page 70: Esp Rna (Fast)
Simulations ESP RNA (Fast) Figure 3.6 ESP RNA (Fast) Screen Press Simulate Protocol The simulation is displayed on the graph. • The green and blue curves represent the relative concentration of linear and supercoiled DNA, respectively. • The red curve represents the density of the CsCl gradient with respect to the right-hand scale. -
Page 71: Recall Simulation
Simulations Recall Simulation Recall Simulation You can recall previously saved simulations that you want to reuse or modify. Select from the Simulate menu. Recall Simulation Select the simulation you want to recall. Press to open the simulation. Open Press to transfer the run parameters to the Settings window. Download NOTE You can modify the program before running it, but changes will not be saved unless you save the... - Page 72 Simulations Recall Simulation 3-10 PN LXP-IM-7AB...
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Page 73: Reference And Log Functions
• A rotor library that lists the Beckman Coulter preparative ultracentrifuge rotors available at the user’s site. • A tube catalog that provides a list of all tubes used in Beckman Coulter preparative ultracentrifuge rotors. • An interactive chemical resistances listing to provide general information about chemical interactions between equipment/accessories used in ultracentrifugation and various commonly used chemicals. -
Page 74: Rotor Catalog Screen
General information about ultracentrifuge rotor selection and use is in Rotors and Tube for Beckman Coulter Preparative Ultracentrifuges (LR-IM); detailed information about the use and care of a specific rotor is contained in the applicable rotor manual. -
Page 75: Rotor Library
Figure 4.3 Selected Rotor Specifications Rotor Library The rotor library is created by the user to list the Beckman Coulter preparative ultracentrifuge rotors that are available at the user’s site. Rotors in the rotor library appear in the rotor drop-down... -
Page 76: Enter Rotor Serial Number Screen
Reference and Log Functions Reference Features To add a rotor to the rotor library: From the Settings screen, press the button. The Select Rotor screen appears. Select Rotor From the Select Rotor screen, press the button. The Select from Rotor Catalog Add Rotor screen appears. -
Page 77: Tube Catalog
General information about tube selection and use is in Rotors and Tubes for Beckman Coulter Preparative Ultracentrifuges (LR-IM), and tube ordering information is in the Ultracentrifuge Rotors, Tubes and Accessories Catalog (BR-8101, available at www.beckmancoulter.com). -
Page 78: Chemical Resistances
CAUTION The information provided in the table is from references, from current literature, or from research done by Beckman Coulter, and is only a guide for the proper selection of materials. No guarantee of safety based on these recommendations is expressed or implied. Many of the chemicals are explosive when concentrated or dry, or are toxic, caustic, allergenic, or carcinogenic. -
Page 79: Chemicals/Upac Name Screen
Reference and Log Functions Reference Features To use the chemicals list: Select from the Reference menu. Chemical Resistances Press the button to see a list of all chemicals represented in the database (see Chemicals Figure 4.8), or select a subset of chemicals to be displayed from the Type of Chemical drop- down menu. -
Page 80: Exporting Data
Reference and Log Functions Exporting Data Figure 4.9 Chemical Resistances Screen Press the button to select another chemical or press to close the window. Chemicals Close Exporting Data The ultracentrifuge automatically saves data associated with each run that may be exported to a memory device. -
Page 81: Optima L-Xp Control Head With Usb Hub
Reference and Log Functions Exporting Data Figure 4.10 Optima L-XP Control Head with USB Hub USB Hub Optima L-XP Ultracentrifuge Speed (RPM): 0 Time(hr:min): 0:00 Temp( C): 24.6 File Setup Simulate Calculate Reference Logs Window Optima L-XP Help BECKMAN Stopped COULTER. -
Page 82: Log Features
Reference and Log Functions Log Features Press the button. The Export Data screen closes when the export is complete, after Clear which the memory device can be safely removed from the USB port. Log Features The Logs menu (see Figure 4.12) provides access to the Instrument Usage Log, Display Last Run Record log, and the Print Last Run Record Automatically option. -
Page 83: Run Record
Reference and Log Functions Log Features automatically printed upon run completion by selecting from Print Last Run Record Automatically the Logs menu (see APPENDIX A, Preinstallation Requirements, for printer hookup instructions). NOTE The settings for a run are logged when the run starts. Figure 4.13 Run Record PN LXP-IM-7AB 4-11... - Page 84 Reference and Log Functions Log Features 4-12 PN LXP-IM-7AB...
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Page 85: Troubleshooting
Maintenance procedures are given in CHAPTER 6, Care and Maintenance. For any problems not covered here, call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada); or contact your local Beckman Coulter Representative for assistance. NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories, before requesting service by Beckman Coulter Field Service. - Page 86 TEMPERATURE Temperature control or vacuum system Check the air inlet (at bottom of malfunctioning the front panel) for obstructions. Call Beckman Coulter Field Service. DRIVE Abnormal change in drive speed or Be sure a rotor is properly overheated drive installed on the spindle;...
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Page 87: Retrieving Your Sample In Case Of Power Failure
Troubleshooting Retrieving Your Sample in Case of Power Failure Retrieving Your Sample in Case of Power Failure If a power failure occurs during a run, the rotor begins to decelerate with the brake off. If the rotor is still spinning above 1000 RPM when power is restored, the instrument will resume operation and the rotor will return to set speed. -
Page 88: Removing The Control Head Cover
Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 5.1 Removing the Control Head Cover Access to Cover Latch Cover Latch Access to Cover Latch To open the top cover, insert a #2 (0.25-inch diameter) Phillips-head screwdriver into the hole located in the front, center of the top cover (see Figure 5.2). -
Page 89: Depressing The Latch To Loosen The Front Panel
Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 5.2 Depressing the Latch to Loosen the Front Panel Top Panel Tabs Front Panel Access Port Front Panel Tabs Figure 5.3 Interior View of Instrument (Panel Removed) Spring-loaded Interlock Pin Door Lock System... -
Page 90: Reinstalling The Front Panel
Troubleshooting Retrieving Your Sample in Case of Power Failure Push down on the interlock pin with your finger (Figure 5.3) while opening the chamber door with your free hand. (If the rotor is still spinning, close the door and wait.) Be very careful not to touch any wires or circuitry. -
Page 91: Care And Maintenance
This chapter contains care and maintenance procedures that should be performed regularly. For maintenance not covered in this manual, call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada); outside the U.S., contact your local Beckman Coulter Representative for assistance. User... -
Page 92: Decontamination
If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions, follow appropriate decontamination procedures as determined by your laboratory safety officer. Refer to Chemical Resistances (IN-175) or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument (or accessories). -
Page 93: Supply List
Supply List Supply List Call Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada), or contact your local Beckman Coulter Representative for information about ordering parts and supplies. A partial list of supplies is given below for your convenience. See the Beckman Coulter Ultracentrifuge Rotors, Tubes &... - Page 94 Care and Maintenance Supply List PN LXP-IM-7AB...
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Page 95: Appendix A: Preinstallation Requirements
Introduction NOTE Do not attempt to install or turn on the power to the Optima L-XP. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. -
Page 96: Bio-Safety Level 3 Installation
A.2) to connect the equipment to earth ground. In regions where the instrument is supplied with an unterminated cord, a plug that meets local electrical and safety requirements must be supplied. (Contact your local Beckman Coulter office for specific information regarding these requirements.) See Table A.1... -
Page 97: Attaching A Printer
NOTE The print driver for the Beckman Coulter supplied Deskjet 940c printer resides in the Optima L-XP eXPert software. If a different printer is used, the print driver will have to be installed by Beckman Coulter Field Service. PN LXP-IM-7AB... -
Page 98: Printer Connection
Preinstallation Requirements Attaching a Printer Figure A.3 Printer Connection PN LXP-IM-7AB... -
Page 99: Appendix B: Remote Access
Technical support for the VNC software is not provided by Beckman Coulter Field Service. This appendix is intended to provide instructions on the use of the Optima L-XP eXPert operating software to enable a connection with the VNC client. Refer to the technical support policy for the VNC software in use. -
Page 100: Vnc License Information
VNC License Information The Optima L-XP eXPert operating software contains VNC Free Edition 4.1.2 by RealVNC Ltd. VNC Free Edition is covered by the GNU General Public License, Version 2. VNC Free Edition 4.1.2 is redistributed within this product in unmodified binary form. Beckman Coulter, Inc. holds the source code for VNC Free Edition 4.1.2 as required by the GNU General Public License, Version 2, and... -
Page 101: Gnu General Public License, Version 2, June 1991
Remote Access GNU General Public License, Version 2, June 1991 GNU General Public License, Version 2, June 1991 Copyright (C) 1989, 1991 Free Software Foundation, Inc. 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA. Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. -
Page 102: Gnu General Public License Terms And Conditions For Copying, Distribution And Modification
Remote Access GNU General Public License, Version 2, June 1991 GNU General Public License Terms and Conditions for Copying, Distribution and Modification 0. This License applies to any program or other work which contains a notice placed by the copyright holder saying it may be distributed under the terms of this General Public License. - Page 103 Remote Access GNU General Public License, Version 2, June 1991 3. You may copy and distribute the Program (or a work based on it, under Section 2) in object code or executable form under the terms of Sections 1 and 2 above provided that you also do one of the following: a.
- Page 104 Remote Access GNU General Public License, Version 2, June 1991 may not distribute the Program at all. For example, if a patent license would not permit royalty- free redistribution of the Program by all those who receive copies directly or indirectly through you, then the only way you could satisfy both it and this License would be to refrain entirely from distribution of the Program.
- Page 105 Remote Access GNU General Public License, Version 2, June 1991 NO WARRANTY 11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM “AS IS”...
- Page 106 Remote Access GNU General Public License, Version 2, June 1991 PN LXP-IM-7AB...
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Page 107: Beckman Coulter, Inc
Any product claimed to be defective must, if requested by Beckman Coulter be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges. - Page 108 2. The drive unit has not been subjected to unequal loading, improper rotor installation, corrosion from material spilled onto the hub or accumulated in the chamber of the instrument. 3. The drive unit has not been disassembled, modified, or repaired, except by Beckman Coulter personnel.
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Page 110: Related Documents
• Chemical Resistances • Using the w2t Integrator • The Use of Cesium • Chloride Curves Gradient Materials • References • Glossary • Available in electronic pdf or CD-ROM by request. www.beckmancoulter.com © 2013 Beckman Coulter, Inc. All Rights Reserved...