Nanolockspray Source - Waters SYNAPT G2 Operator's, Overview And Maintenance Manual

Hot gas from the APCI probe passes between the sample cone and the corona
pin. Mobile phase molecules rapidly react with ions generated by the corona
discharge to produce stable reagent ions. Sample molecules introduced into
the mobile phase react with the reagent ions at atmospheric pressure and
typically become protonated (in the positive ion mode) or deprotonated (in the
negative ion mode). The sample and reagent ions then pass through the
sample cone and into the mass spectrometer.
Combined electrospray and atmospheric pressure chemical
ionization
In combined electrospray and atmospheric pressure chemical ionization
(ESCi) mode, the standard ESI probe is used in conjunction with a corona pin
to allow alternating acquisition of ESI and APCI ionization data, facilitating
high-throughput processing and wider compound coverage.

NanoLockSpray source

The NanoLockSpray source allows electrospray ionization performed in the
flow rate range of 5 to 1000 nL/min.
For a given sample concentration, the ion currents for similar experiments
approximate to those in normal flow rate electrospray. However, because
sample consumption is greatly reduced, the sensitivity gains are significant
when you adopt similar scan parameters. Lock-mass correction with the
NanoLockSpray source works in the same way as the LockSpray source does
with ESI.
The NanoLockSpray source enclosure consists of a sprayer—either universal,
borosilicate glass capillary, or CE (see below)—mounted on a ZSpray,
three-axis manipulator.
The combined unit is mounted on the NanoFlow™ stage, which runs on a pair
of guide rails, with two defined positions.
A light within the source provides illumination for the spray, which you can
observe using the video camera mounted on the corner of the source housing.
Instrument sources 1-7