Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual
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Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual

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ACQUITY UPLC
Column Compartments Operator's Overview
and Maintenance Information
Revision C
Copyright © Waters Corporation 2010
2011
All rights reserved

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Summary of Contents for Waters ACQUITY UPLC

  • Page 1 ACQUITY UPLC Column Compartments Operator’s Overview and Maintenance Information Revision C  Copyright © Waters Corporation 2010 2011 All rights reserved...

  • Page 2: Copyright Notice

    Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

  • Page 3: Table Of Contents

    Using the column compartments ..............98 Control panels ....................98 Resolving column compartment leak sensor errors ......... 99 Maintaining the column compartments ............. 110 Contacting Waters technical service............... 110 Maintenance schedule ..................112 Maintenance considerations................113 Configuring maintenance warnings ............... 113 Replacing the column heater’s leak sensor ............
  • Page 4 Replacing the column heater/cooler fuse (ACQUITY UPLC 30-cm CHC)..139 Cleaning the instrument’s exterior (all models) ..........140 Table of Contents...

  • Page 5: Overview

    ® UPLC column compartments help to ensure precise, reproducible separations by controlling the column temperature. If you are using an ACQUITY UPLC system with two dimensional Note: applications, refer to the ACQUITY UPLC Systems with 2D Technology Capabilities Guide. Column compartment types...
  • Page 6 A receptacle on the column heater’s right-hand side receives the column's eCord™ chip. The eCord column chip stores column manufacturing and usage data, which you can access from the ACQUITY UPLC Console. The column heater does not have its own power supply; it draws power from the sample manager.
  • Page 7 The following diagram shows the location of the column heater in the ACQUITY UPLC system. Bottle tray Detector Column heater (CH-A) Sample manager - flow through needle / TP03240 Sample manager - fixed loop Solvent manager Overview...
  • Page 8 Column heater, major components: The following diagram shows the column heater’s major components. Location of Column Active preheater column compartment tray assembly Active preheater Fitting retainer Column heater components: Component Description Column Contains the column, column heater, in-line filter, and compartment active preheater or column stabilizer assembly.
  • Page 9 Column heater components: (Continued) Component Description Column stabilizer Passively heats the incoming solvent before the solvent (optional) enters the column. Drip tray Captures any leakage from the column or column connections and routes it into the drip management system. 30-cm Column heater (CH-30A) The optional 30-cm column heater heats the column compartment to any temperature, from 20ºC to 90 ºC, that is at least 5 ºC above ambient.
  • Page 10 150 mm, or less, length and HPLC columns of 4.6 mm, or less, ID and 300 mm, or less, length. For columns that exceed 4.6 mm ID, use the ACQUITY UPLC 30-cm column heater/cooler with the appropriate compatibility kit.
  • Page 11 4 and 90 °C. The temperature of each column trough can be independently set in 0.1 °C increments. The column manager can be used for column switching applications as well as two dimensional applications. For two dimensional applications, see the ACQUITY UPLC Systems with 2D Technology Capabilities Guide. Overview...
  • Page 12 Receptacles on the column manager’s front panel receive the column eCord™ chips. The eCord column chip stores column manufacturing and usage data, which you can access from the ACQUITY UPLC Console. Column manager major components The following diagram shows the column manager’s major components.
  • Page 13 Column manager and auxiliary column manager components: Component Description Column Contains the column, column heater, in-line filter, and compartment active preheater assemblies. The column compartment insulates the column from changes in ambient temperature. In-line filter Protects the analytical column against contamination by trapping impurities before they reach the column.
  • Page 14 To accommodate additional 100- or 150-mm columns, the ACQUITY UPLC system can include one or two auxiliary column manager (CM-Aux) modules (a maximum of six columns per system). These configurations are available for an ACQUITY UPLC system including a CM-Aux: •...
  • Page 15 Examples of column manager configurations: Dual stack Sample CM-Aux Manager CM-A Solvent Single stack Manager CM-Aux CM-Aux Sample Manager CM-A Solvent Manager Sample Manager CM-Aux CM-Aux CM-A CM-A Solvent Manager CM-Aux Sample Manager CM-Aux Solvent CM-A Manager Sample CM-Aux Manager CM-A Solvent Sample...
  • Page 16 The ACQUITY 30-cm column heater/cooler is a combination forced-air-convection heater and cooler with a temperature range of 20 to 65 °C. 30-cm column heater/cooler: The ACQUITY UPLC 30-cm column heater/cooler offers these features for ease of use: • Adjustable column clips that slide, for easy column mounting.
  • Page 17 • Single-piece column compartment door and hinge assembly that allows tubing to enter or exit the column compartment from all four sides. • Columns with dimensions of up to 7.8 mm diameter. Convection grid Column clip Temperature port Column clip rails Compartment door clasp Door gasket Compartment...

  • Page 18: Preparing The Column Compartments

    Preparing the column compartments Installation recommendations for fittings To avoid the harmful effects of personal contact with Warning: fittings contaminated with biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when reinstalling the fittings. The system uses gold-plated compression screws and two-piece ferrules. See the diagram below for assembly orientation.
  • Page 19 When tightening system fittings, consult the following table. Installation recommendations for ACQUITY UPLC column compartment fittings: Fitting Recommended tightening Reusable finger-tight Finger-tight, plus 1/6-turn 1/6-turn TP02728 Reusable finger-tight Finger-tight, plus up to 1/6-turn; if leaking, tighten another 1/8-turn Collet removal tool...
  • Page 20 Installation recommendations for ACQUITY UPLC column compartment fittings: (Continued) Fitting Recommended tightening Stainless steel (gold-plated) fitting with Finger-tight, plus up to long flats and 2-piece stainless steel 1/6-turn using wrench ferrule 1/6-turn TP02713 Long flats 2-piece ferrule Dual-threaded finger-tight fitting with 1.

  • Page 21: Installing The Standard Column Heater

    Installing the optional MS extension bracket If your system includes a mass spectrometer, use the optional MS-bracket to position the column heater to the right of the ACQUITY UPLC system stack. Doing so reduces the length of the tubing that extends to the mass spectrometer inlet.
  • Page 22 ® – Phillips screws – Cable clamp nut • Drain tubing • Gloves: clean, powder-free, chemical-resistant Required tools • 1/4-inch open-end wrench • 4-mm open-end wrench • 3-mm hex wrench • Phillips screwdriver • Small flat screwdriver • T20 TORX driver MS-bracket kit major components: Column heater housing TP03252...
  • Page 23 To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment internal temperature to ensure all components are cool. To prevent contaminating system components, wear clean, Caution: chemical-resistant, powder-free gloves when preparing the column...
  • Page 24 Remove the column stabilizer assembly, if fitted. Using the Phillips screwdriver, loosen the two screws that fasten the column stabilizer assembly to the column compartment tray. Thermal gasket TP03425 Location of Phillips screws Remove the column stabilizer assembly from the thermal gasket. Remove the thermal gasket from the column tray.
  • Page 25 Using a small screwdriver, gently pry out the active preheater retainer, and remove it from the column compartment. Gently compress retainer here Remove any solvent lines routed behind the column heater door. Detach the leak sensor connector from the front of the sample manager, if fitted.
  • Page 26 11. Gently pull the right-hand side of the column heater toward you, and then slide it to the right, to unseat the tab on the left-hand side of the column heater from the device’s chassis. 12. Remove the 25-pin cable from the receptacle on the back of the column heater.
  • Page 27 Remove the Phillips screw that secures the column heater’s rear enclosure to the sample manager chassis. Remove the column heater’s rear enclosure from the sample manager chassis. Secure the retainer bracket, supplied in the column heater startup kit, onto the sample manager chassis using the TORX screw provided in the kit.
  • Page 28 Install the Phillips screw that secures the column heater’s rear enclosure to the sample manager chassis. Replace any other instruments and the solvent tray above the column heater’s rear enclosure. 15. Install the MS-bracket on the sample manager’s chassis: Insert the tab on the left-hand side of the MS-bracket into the slot on the left-hand side of the sample manager chassis.
  • Page 29 Route the 25-pin cable behind the MS-bracket and through the slot on the right-hand side of the bracket. Route the 25-pin cable through this slot Install the TORX screw in the tab, and tighten it slightly. Hold the left-hand side of the bracket into the slot, and guide the MS-bracket tab on the right-hand side into the cutout in the sample manager’s chassis.
  • Page 30 16. Attach the column heater housing onto the MS-bracket using the hinge: Attach the short side of the hinge to the MS-bracket using two TORX screws. Attach the long side of the hinge to the column heater housing using two TORX screws. MS-bracket Column heater housing 17.
  • Page 31 Using two TORX screws, attach the height adjustment bracket to the MS-bracket. TORX screws Height adjustment bracket MS-bracket Using two TORX screws, attach the hinge assembly on the column heater housing to the height adjustment bracket. Swing the column heater housing, aligning it with the front of the mass spectrometer, and ensure that the column heater is at the correct height for the mass spectrometer inlet.
  • Page 32 20. Install the leak sensor: Align the leak sensor’s T-bar with the slot in the left-hand side of the MS-bracket drip tray, and slide the leak sensor into place. Leak sensor T-bar TP02892 Slot Route the leak sensor cable through the slot in the column heater housing.
  • Page 33 Tighten the cable clamp nut with the 4-mm wrench. 25-pin cable routed through slot 4-mm cable clamp nut on stud Cable clamp 22. Install the column heater in its housing: Insert the tab on the left-hand side of the column heater into the slot on the left-hand side of the housing.
  • Page 34 23. Push the 25-pin cable into the socket on the back of the column heater, and then tighten the thumbscrews to secure the cable to the socket. Thumbscrews 24. If you installed the optional leak sensor, plug the connector into the receptacle located behind the column heater.
  • Page 35 28. Slide the column compartment tray to the right. Column compartment tray 29. If desired, install the active preheater assembly: Gently compress the active preheater assembly retainer between your thumb and forefinger, and push it into the left-hand side of the column compartment until it snaps into place.
  • Page 36 tubing to the mass spectrometer inlet is routed through the groove on the right-hand side of the column compartment. If you installed the active preheater, connect the column inlet to the active preheater by holding the active preheater’s reusable compression fitting in place, rotating the column onto the fitting until it is snug, and then tightening an additional 1/6 turn.
  • Page 37 Attach the eCord fob to the receptacle on the side of the column heater. Receptacle eCord fob Close the column heater door. Installing the optional column stabilizer assembly The column heater’s optional column stabilizer reduces sensitivity to ambient temperature swings by transferring heat (via conduction) from the column heater’s film element through the thermal gasket to the column inlet tubing.
  • Page 38 If you are running an HPLC or UPLC method that was originally developed for use with a column stabilizer, you must continue to use the column stabilizer when you run that method on the ACQUITY UPLC system. To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them.
  • Page 39 Required materials • Gloves: clean, powder-free, chemical-resistant • 50/100-mm or 150-mm column stabilizer kit: – Collet and compression screw multi-tool (collet separator) – Column stabilizer assembly – PEEK tubing for connecting the column to the detector’s flow cell – 3 Phillips screws (1 is a spare) –...
  • Page 40 Remove the active preheater assembly from the retainer in the column compartment. Active preheater assembly Using a needle-nose pliers, gently compress the top and bottom of the active preheater retainer, and pull it out of the column compartment. Gently compress retainer here Remove the clear, protective backing from the thermal gasket, and with the thermal gasket oriented vertically, center it between the two...
  • Page 41 Press the thermal gasket into the column compartment tray, ensuring the gasket is centered and formed into the bottom of the tray, with equal lengths on both sides. Firmly press the column stabilizer assembly into the thermal gasket, ensuring the thermal gasket uniformly envelops the sides of the column stabilizer and does not bunch beneath it.
  • Page 42 Carefully route the column-stabilizer-inlet tubing through the groove on the side of the tray. To avoid stripping the threads, do not overtighten Caution: screws. 10. Insert the Phillips screws into the threaded holes, and tighten them gently using the Phillips screwdriver. 11.

  • Page 43: Installing The 30-Cm Column Heater

    Installing the 30-cm column heater If your system includes a 30-cm column heater, follow the procedure below to install it. To avoid damaging the electronic components of column Caution: heaters fitted with a rear DSUB cable, unplug the cable from the sample manager before performing this procedure.
  • Page 44 Using the T20 TORX screwdriver, remove the top screw from the right-hand side of the sample manager; remove the bottom screw from the right-hand side of the solvent manager. Right-hand side of system stack: Detector Sample manager Remove screws Solvent manager...
  • Page 45 Insert the two M4 × 50-mm long screws into the side-mount shafts, from the side with the knurled, side-mount collars. Insert M4 × 50-mm long screws here Screw the side-mount shafts into the top, right-hand side of the sample manager and the bottom, right-hand side of the solvent manager. Detector Sample manager Install side-mount...
  • Page 46 Mount the CH-30A by sliding the large key-hole slots onto the side mount shafts. Key-hole slots Turn the knurled, side-mount collars counter-clockwise, to pinch the CH-30A sheet metal chassis within the mount for a secure fit. Knurled side mount collar Connect one end of the pin cable to the socket on the 30-cm column heater’s rear panel.
  • Page 47 Connect the other end of the pin cable to the socket on the sample manager’s rear panel. Connect the pin cable between these sockets Sample manager - FTN 30-cm column heater 10. Connect the drain tubing to the barbed fitting on the bottom front of the column heater.
  • Page 48 Moving the preheater key When the CH-30A is shipped, the preheater key is installed in the upper, active preheater control port, thus readying the active preheater for use with a 30-cm column heater and 300-mm (30-cm) column. If you intend to use the active preheater with shorter tubing and a shorter column, you must move the preheater key to the lower control port.

  • Page 49: Installing The Column Manager

    Gently press the preheater key assembly into the upper or lower-control port in the column compartment. Insert this end into control port Using a needle-nose pliers, install the active preheater retainer, in the control port where the active preheater will be installed. Insert the active preheater intended for use.
  • Page 50 Two-column configuration, with inlet switching-valve on the right-hand side: To detector Outlet Inlet switching switching Plug valve valve Column position 1 Column manager Column position 2 Sample manager Injector See the appropriate site preparation guide for information regarding Note: which tubing and valve kit to use. Two-column configuration plumbing connection details: Part number Length...
  • Page 51 Two-column configuration plumbing connection details: (Continued) Part number Length From (inches) H-Class I-Class CM-A inlet Column 1 inlet 12.5 205000730, 205000894, valve, port 3 CM-A inlet Column 2 inlet 12.5 205000730, 205000894, valve, port 4 Column 1 outlet CM-A outlet 430002683, SST 430003151, SST valve, port 3...

  • Page 52: Installing One Cm-Aux Module, In A Single-Stack Configuration

    Notes: • The two-column configuration for the ACQUITY UPLC I-Class system uses low-volume tubing with high-pressure fittings. • When installing the active preheater on the column inlet, use a wrench to tighten the gold fitting onto the column an additional 1/4-turn.
  • Page 53 Disconnect the power and Ethernet cables from the rear panel of the CM-A and detector. To avoid injuries arising from personal contact with Warning: spilled solvent, remove the solvent bottles from the solvent tray before removing the solvent tray. Remove all solvent bottles from the bottle tray. Remove the solvent tray from the system stack.
  • Page 54 10. Place the solvent bottles in the solvent tray. Front view of repositioned instruments: Bottle tray Detector CM-Aux CM-A Sample manager Solvent manager 11. Connect the power cables to the CM-Aux, CM-A, and detector. 12. Connect the external control cable between the CM-A’s upper auxiliary connector and the CM-Aux.
  • Page 55 13. Connect the Ethernet cables to the rear panel of the CM-A and detector. Rear view of repositioned instruments: Bottle tray Detector CM-Aux External control cable CM-A Sample manager Solvent manager Setting up waste management To set up the waste management components: Route a waste line from the barbed fitting on the rear of the solvent tray to a suitable waste container.
  • Page 56 Install the 4.125-inch, convoluted drip tube (part number 430002826) from the drip tray of the CM-Aux to the front waste port of the CM-A’s drip block. To avoid leaks, ensure that the convoluted drip tube is fully inserted Tip: into the front waste port of the CM-A’s drip block. CM-Aux Convoluted drip tube CM-A drip block...
  • Page 57 Plumbing the CM-A and CM-Aux To avoid damaging the tubing and column compartment cover latches, Tip: ensure the tubing is routed through the grooves in the sides, top, and bottom of the column compartment. Location of grooves in sides, top, and bottom of column compartment: Grooves(3) on right side and top of column compartment...

  • Page 58: Installing Two Cm-Aux Modules In A Multiple-Stack Configuration

    Installing two CM-Aux modules in a multiple-stack configuration These configurations can support either four or six columns (in any combination of 50-, 100-, or 150-mm columns). The inlet valve is located on the left-hand side of the column manager. Required materials •...
  • Page 59 Reposition the instruments for the new configuration. Set up waste management, as needed. Exchange the valve cartridges. Install new magnetic diagrams (see page 68). Install the tubing. Configure all columns in the software. Removing the fittings from the inlet and outlet valves To remove the fittings from the inlet and outlet valves: Power-off the column manager (CM-A) and detector.
  • Page 60 Tilt the column compartment cover forward, just enough to clear the valve drip tray. Latch pins Valve drip tray Latches Column compartment cover Pull the column compartment cover up and away from the CM-A. For each fitting installed on the inlet and outlet valves of the CM-A, mark the fitting with the number of the port to which it is connected.
  • Page 61 Installing the extended feet on the bottommost column module The extended feet allow room for installation of the swivel drain. Verify that the extended feet are installed on the bottommost column module, the CM-A or the CM-Aux, in the system module configuration you choose. To install the extended feet on a CM-A or a CM-Aux: Turn the CM-A or CM-Aux so that it is resting on it’s side.
  • Page 62 Repositioning instruments in the system stack When repositioning the instruments, ensure the bottommost Requirement: column module, the CM-A or the CM-Aux, is fitted with the drip tray equipped with a leak detector (see page 59). To reposition the instruments in the system stack: Power-off the column manager (CM-A) and detector.
  • Page 63 Setting up waste management To set up the waste management for a lower CM-Aux: Route the detector waste tube to the solvent manager drip tray. Route the outlet of the detector flow cell to waste (using the backpressure regulator) or to another in-line detector. Using the T20 TORX driver, loosen the upper and middle screws that secure the CM-A drip block to the module.
  • Page 64 Using the T20 TORX driver, remove the lower screw, which secures the CM-A’s drip tray to the module. Lower screw Remove the drip block assembly from the CM-A, separate the drip tray from the drip block, and then set the drip tray and block aside. Drip block Drip Tray Repeat...
  • Page 65 Insert the CM-Aux drip tray into the CM-A drip block, and then insert the CM-A drip tray into the CM-Aux drip block. CM-A drip block CM-Aux drip block CM-A drip tray CM-Aux drip tray Reinstall the CM-A drip block assembly (drip block and CM-Aux drip tray) onto the CM-A module, and then secure it in place using the three T20 TORX screws.
  • Page 66 To avoid leaks, ensure that the convoluted drip tube is fully inserted Tip: into the front, waste port of the CM-A drip block. Upper CM-Aux Convoluted drip tube (4.125-inch) CM-A drip block 10. Install the 2.0-inch convoluted drip tube (part number 430002827) onto the CM-A drip tray.
  • Page 67 11. Insert the open end of the convoluted tube into the front waste port of the lower CM-Aux’s drip block, and carefully reinstall the drip block assembly onto the lower CM-A, as shown in the figure below. CM-A drip tray Lower CM-Aux drip block Tips: •...
  • Page 68 Installing the swivel drain and waste tubing To install the swivel drain and waste tubing: Push the latches atop the CM-A’s column compartment cover inward, to disengage them from the latch pins. Tilt the column compartment cover forward, just enough to clear the valve drip tray.
  • Page 69 To avoid leaks, ensure the O-ring is properly seated in Caution: the swivel drain cup. Install the swivel drain cup on the bottom of the drip tray, and thread the mounting screw through the hole in the top of the drip tray. Mounting screw and washer Hole in top of drip tray CM-A drip tray...
  • Page 70 To exchange the CM-A inlet and outlet switching-valve cartridges: In the ACQUITY UPLC Console software, select Column Manager from the system tree. In the column manager information window, click Interactive Display. In the Column Manager Interactive Display dialog box, click Control.
  • Page 71 Ensure the inlet switching-valve is set to Waste. To change the setting to Waste, click System and select Waste. Tip: Switching-valve cartridge: Lock ring Lock ring stop 2-mm hex screw Stator ID Use the 2-mm hex wrench to remove the hex screw at the 10 o’clock position on the switching-valve cartridge.
  • Page 72 Requirement: as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service. 13. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench.
  • Page 73 Use a pen to coil any excess tubing on any of the connections. Tip: When all valve connections are made, rotate the valve’s lock ring counterclockwise, to the 12 o’clock position. Push the switching-valve assembly inward until it stops. Plug all unused ports on the inlet and outlet switching valves using pin plugs.
  • Page 74 Four-column configuration, with inlet switching-valve on the left-hand side: To detector Tubing Router Column position 1 Column Manager-Aux Column position 2 From Drip tray inject Drip tube valve Inlet Outlet switching switching valve valve Column position 3 Column Manager Column position 4 Drip tray with Extended feet...
  • Page 75 Six-column configuration, with inlet switching-valve on the left-hand side: To detector Tubing Router Column position 1 Column Manager-Aux Column position 2 From Drip tray inject Drip tube valve Inlet Outlet switching switching valve valve Column position 3 Column Manager Column position 4 Drip tray Drip tube...
  • Page 76 Four-column configuration in a single CM-Aux (50-mm columns), with inlet switching-valve on the right-hand side: Outlet Inlet switching switching valve valve Column Column position 1 position 2 Column manager Column Column position 3 position 4 Sample manager Drip tray with leak detector Injector valve...
  • Page 77 Four and six column configuration plumbing connection details: (Continued) Part number Length From MP35N (inches) Stainless steel (H-Class Bio) CM-A inlet Column 2 12.5 279002002, APH 279002003 valve, port 2 inlet (reorder part (reorder part number number 205000730) 205000756) CM-A inlet Column 3 12.5 279002002, APH...
  • Page 78 Four and six column configuration plumbing connection details: (Continued) Part number Length From MP35N (inches) Stainless steel (H-Class Bio) Column 3 CM-A outlet 430002683, 430002684 outlet valve, port 3 tubing, (reorder part .004-inch ID number (reorder part 700005483) number 700005478) Column 4 CM-A outlet 430002683,...
  • Page 79 Four and six column configuration plumbing connection details: (Continued) Part number Length From MP35N (inches) Stainless steel (H-Class Bio) Sample CM-A inlet 430002599, tube 430002641 manager valve, center assembly, (reorder part injector valve, port (I) .004-inch ID × number port 6 19.0-inch long 700005484) (reorder part...
  • Page 80 Identifying switching valves and active preheaters The following table lists the features that identify standard and bio system switching valves and active preheaters. Tubing contained in the SST or MP35N column tubing kits does not Note: have an identification sleeve. Switching valve and active preheater identification features: H-Class Bio H-Class system...
  • Page 81 Installing dual-threaded fittings To install the dual-threaded fittings: Loosen the stainless steel cap nut from the dual-threaded, finger-tight fitting. APH dual-threaded fitting with stainless steel cap nut: Gold Stainless steel fitting cap nut Ferrule Stainless steel tubing Ensure the stainless steel tubing protrudes beyond the PEEK ferrule. Apply the axial load towards the column hardware and insert the stainless-steel tubing with the ferrule into the detail of the column hardware.
  • Page 82 Configuring the instruments To configure the instruments: Power-on the CM-A and detector. From the ACQUITY UPLC Console system tree, select TUV detector > Control > Reset TUV. From the ACQUITY UPLC Console system tree, select Column Manager > Configure > Instrument.
  • Page 83 Six-column configuration: When using Empower 1154 or Empower 2 on a system containing Note: CM-A and CM-Aux modules, you cannot select positions 5 and 6 from the column selection field of the sample queue. Instead, create an instrument method for a specific column position (5 or 6, for example) and save it with a unique name.
  • Page 84 3 and 4. In the Configuration Confirmation window, click OK, reboot the CM-A, and then click OK to restart the ACQUITY UPLC Console. From the ACQUITY UPLC Console, click Configure > Arrange Instrument Modules, and then click and drag the instrument modules to appropriate locations, if necessary.
  • Page 85 LED, also on the left-hand side of the front panel, is steady green after the CM-Aux is configured (in the ACQUITY UPLC Console) and the CM-A is rebooted. The power LED is unlit after the CM-Aux is removed from the instrument configuration and the CM-A is rebooted.

  • Page 86: Installing The Acquity 30-Cm Column Heater/Cooler

    Connect the column inlet and outlet of the column in position 1. Connect the eCord to the port on the right side of the upper CM-Aux. Run flow through the column with backpressure to ensure that there are no leaks. Install the columns in the other positions as desired and test for leaks.
  • Page 87 Using the T20 TORX screwdriver, remove the top screw from the right-hand side of the sample manager, and the bottom screw from the right-hand side of the solvent manager. Right-hand side of system stack: Detector Sample manager - FTN Remove screws Solvent manager Preparing the column compartments...
  • Page 88 Mount the rear tab of the adaptor bracket onto the ground stud at the rear of the sample manager. Bracket Ground stud...
  • Page 89 Insert two M4 × 16 screws into the slots on the bracket’s left-hand panel, leaving the first screw loose until both screws are inserted. Then tighten both screws. M4 side panel spacers with cover screws Ground stud M4 × 16 screws ®...
  • Page 90 Insert the three, M4, blue, cover screws through the three side-panel spacers, and then screw them into the three threaded holes in the bracket’s center panel. TP02939 Spacer Cover screw Mount the column heater/cooler onto the bracket, fitting the column heater/cooler mounting holes over the M4 cover screws.

  • Page 91: Installing The Optional Column In-Line Filter

    Load the clip into the bit driver in the orientation desired, and then snap into the bracket. Tubing clip 1/4-inch bit driver TP02945 Installing the optional column in-line filter To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them.
  • Page 92 Required materials • Column in-line filter • Gloves: clean, powder-free, chemical-resistant • Reusable fitting Required tools 5/16-inch open-end wrench For instruction on how to replace the filter in the column heater’s in-line filter unit, see page 136. To prevent contaminating system components, wear Caution: powder-free, non-latex gloves when connecting tubing and tightening fittings.

  • Page 93: Installing The Column

    Remove the column in-line filter unit from its packaging, and then push the outlet end of the active preheater or column stabilizer tubing assembly into the in-line filter inlet until it stops. Installing the in-line filter: Flow From column stabilizer In-line filter unit Column tubing assembly...
  • Page 94 Installing the column (CH-A, CM-A, CM-Aux, and CH-30A) Waters ships the CH-30A with a tubing kit that includes several column Note: inlet and exit tube options. ACQUITY instrument stack configurations can vary, so you must configure the shortest possible column exit tube to the detector.
  • Page 95 Push the outlet end of the column in-line filter unit into the column inlet until it stops. To avoid damaging the tubing, use only your fingers to Caution: tighten the fittings. Holding the finger-tight reusable fitting in place, rotate the column onto the fitting until it is snug, and then tighten it up to an additional 1/6-turn.
  • Page 96 To connect the PEEK tubing to the detector: Remove the protective cover from the PEEK tubing from the flow cell inlet in the detector. Ensure the label on the flow cell inlet tubing matches the type of detector and flow cell in your system. Attach the 0.004-inch or .0025-inch ID inlet tubing (included with the flow cell or the stabilizer kit) to the column outlet.
  • Page 97 Attach the eCord fob to the receptacle: • Column heater – the receptacle is located on the right-hand side. Heater compartment receptacle eCord fob • 30-cm column heater – the receptacle is located on the top of the device’s chassis. Receptacle Preparing the column compartments...
  • Page 98 • Column manager – the receptacle is located inside the front panel. Consult the figure below to determine the correct placement. 2 column configuration: Top column 4 column configuration: 4 column configuration: Top right column Top left column eCord fob 4 column configuration: 2 column configuration: Bottom column Bottom left column...
  • Page 99 After discontinuing power, wait 10 seconds before attempting to disconnect an assembly. Installing the columns (ACQUITY UPLC 30-cm CHC) You can install multiple chromatographic columns within the compartment of the column heater or column heater/cooler. The table below shows the possible column configurations.
  • Page 100 ACQUITY UPLC 30-cm CHC column configurations: Quantity Description 300-mm length column: 7.8-mm ID, or less, with a column guard of 20-mm, or less 300-mm length column: 7.8-mm ID, or less, with column guards of 20-mm, or less 150-mm length column: 7.8-mm ID, or less, without column guards 50-mm length column: 7.8-mm ID, or less, without column...
  • Page 101 Tubing retainers provided along the left-hand side, bottom, and top of the column compartment hold tubing in place. Use these retainers to organize tubing and keep it orderly. Tubing retainers: The heater or heater/cooler column compartment functions only when you can properly close the door.

  • Page 102: Using The Column Compartments

    Using the column compartments To avoid explosion, do not use the column heater with solvents Warning: whose autoignition temperatures are below 150 C. To avoid breathing harmful fumes or vapors, when using Warning: hazardous solvents, run the system in a hooded environment that provides a sufficient air exchange rate.

  • Page 103: Resolving Column Compartment Leak Sensor Errors

    Nonabrasive, lint-free wipes To resolve a column heater leak sensor error (door in closed position): In the ACQUITY UPLC Console’s Leak Sensors dialog box, determine which of the sample manager’s two leak sensors detected a leak. If the message reads “Leak Detected (Column)”, open the column heater’s door.
  • Page 104 Disconnect the column inlet, active preheater, or column stabilizer tubing from injector port 6 of the sample manager. Location of column, active preheater, or column-stabilizer-inlet tubing Disconnect the column-outlet PEEK tubing from the detector inlet. Remove any solvent tubing routed behind the column heater door. Power-off the sample manager, and detach the leak sensor connector from the front of the column heater.
  • Page 105 Gently pull the right-hand side of the column heater toward you, and then slide it rightward, to unseat the tab on the left-hand side of the column heater from the instrument chassis. To avoid damaging the leak sensor, do not grasp it by the Caution: ribbon cable.
  • Page 106 11. Use a nonabrasive, lint-free wipe to dry the leak sensor prism. Prism Lint-free wipe TP02891 12. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. Leak sensor reservoir Rolled up lint-free wipe...
  • Page 107 13. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area. Leak sensor reservoir Cotton swab Using the column compartments...
  • Page 108 15. If you detached the connector from the front of the instrument, reattach it, and power-on the sample manager. 16. In the ACQUITY UPLC Console, select Sample Manager FTN from the system tree. 17. In the sample manager information window, click Control > Reset SM, to reset the sample manager.
  • Page 109 To avoid damaging the leak sensor, do not grasp it by the Caution: ribbon cable. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it. Serrations Use a nonabrasive, lint-free wipe to dry the leak sensor prism. Prism Lint-free wipe TP02891...
  • Page 110 Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. Rolled up lint-free wipe Leak sensor reservoir With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area.
  • Page 111 T-bar Leak sensor installed in reservoir In the ACQUITY UPLC Console, select Sample Manager FTN from the system tree. 10. In the sample manager information window, click Control > Reset SM, to reset the sample manager. To resolve a column manager leak sensor error: Power-off the column manager.
  • Page 112 Detach the leak sensor connector from the front of the column manager by pressing down on the tab and pulling it out of the receptacle. Press down on tab to release connector Leak sensor connector To avoid damaging the leak sensor, do not grasp it by the Caution: ribbon cable.
  • Page 113 Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. TP02892 T-bar Slot in leak sensor reservoir Leak sensor installed in reservoir 10. Reattach the connector to the receptacle on the front of the instrument, and power-on the column manager.

  • Page 114: Maintaining The Column Compartments

    11. In the ACQUITY UPLC Console, select Column Manager from the system tree. 12. In the column manager information window, click Control > Reset CM, to reset the column manager. Maintaining the column compartments Under normal operating conditions, the column compartments do not require any routine maintenance.
  • Page 115 Be prepared to provide the serial numbers of the instruments in your system when you contact Waters customer support. To view instrument information: In the ACQUITY UPLC Console, select an instrument from the system tree. Click Configure > View module information. The Module Information dialog box displays this information: •...

  • Page 116: Maintenance Schedule

    Maintenance schedule Waters recommends that you perform the following routine maintenance on the column compartments to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents such as buffers, perform these maintenance tasks more frequently.

  • Page 117: Maintenance Considerations

    Maintenance considerations Safety and handling Observe these warning and caution advisories when you perform maintenance operations on your system. To avoid the harmful effects of personal contact with solvents, Warning: including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use.

  • Page 118: Replacing The Column Heater's Leak Sensor

    For information on setting maintenance warnings, consult the ACQUITY UPLC Console online Help. Replacing the column heater’s leak sensor Perform this procedure if you must replace the leak sensor. To avoid personal contamination from contact with Warning: biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when performing this procedure.
  • Page 119 Open the sample manager door, gently pulling its right-hand edge toward you. Press down on the tab to detach the leak sensor connector from the front of the instrument. Press down on tab to release connector Leak sensor connector Disconnect the column-outlet PEEK tubing from the detector inlet. Remove any solvent lines routed behind the column heater door.
  • Page 120 Gently pull the right-hand side of the column heater toward you, and then slide it to the right, to unseat the tab on the left-hand side of the column heater from the instrument chassis. To avoid damaging the leak sensor, do not grasp it by the Caution: ribbon cable.
  • Page 121 11. Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. TP02892 T-bar TP02909 Slot in leak sensor reservoir Leak sensor installed in reservoir 12. Plug the leak sensor connector into the front of the instrument. Maintaining the column compartments...
  • Page 122 13. Insert the tab on the left-hand side of the column heater into the slot on the left-hand side of the instrument chassis. Slot 14. Hold the left-hand side of the column heater into the slot, and guide the eCord receptacle tab on the right-hand side into the cutout in the instrument chassis.

  • Page 123: Replacing The 30-Cm Column Heater's Leak Sensor

    17. Connect the column-outlet PEEK tubing to the detector inlet. 18. Power-on the sample manager. 19. In the ACQUITY UPLC Console, select Sample Manager FTN from the system tree. 20. In the sample manager information window, click Control > Reset SM, to reset the sample manager.
  • Page 124 To avoid personal contamination from contact with Warning: biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required materials • Gloves: clean, powder-free, chemical-resistant • New leak sensor To replace the column manager’s or auxiliary column manager’s leak sensor: Power-off the column manager.
  • Page 125 Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. TP02892 T-bar Slot in leak sensor reservoir Leak sensor installed in reservoir Maintaining the column compartments...

  • Page 126: Replacing The Column Manager Switching-Valve Cartridge

    Front of instrument Leak sensor connector receptacle Power-on the column manager. In the ACQUITY UPLC Console, select Column Manager from the system tree. 10. In the column manager information window, click Control > Reset CM, to reset the column manager.
  • Page 127 • 2-mm Allen wrench (startup kit) To replace the column manager switching-valve cartridge: In the ACQUITY UPLC Console, select Column Manager from the system tree. In the column manager information window, click Interactive Display. In the column manager interactive display dialog box, click Control.
  • Page 128 Requirement: as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service. 13. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm Allen wrench.

  • Page 129: Replacing The Column

    Replacing the column To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment internal temperature to ensure all components are cool. To prevent contaminating system components, wear clean, Caution: chemical-resistant, powder-free gloves when replacing the column.
  • Page 130 Tilt the column compartment cover forward just enough to clear the valve drip tray. Latch pins Valve drip tray Latches Column compartment cover Pull the column compartment cover up and away from the column manager or auxiliary column manager. Do these things if the column compartment has an active preheater: Remove the active preheater assembly from the retainer.

  • Page 131: Replacing The Active Preheater Assembly

    While holding the gold active preheater fitting, rotate the column off the fitting. If you are installing the active preheater in a system Requirement: with pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), use the 1/4-inch and 5/16-inch open-end wrenches to disconnect the compression fitting that attaches the active preheater assembly to the optional in-line filter or to the column.
  • Page 132 To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment’s internal temperature to ensure all components are cool. To prevent contaminating system components, wear clean, Caution: chemical-resistant, powder-free gloves when replacing the active...
  • Page 133 • Column manager – disconnect the tubing from the appropriate injector port of the switching-valve. The port varies depending on which active preheater you are replacing. See the figures on page page page page 71, and page • Auxiliary column manager – disconnect the tubing from the appropriate injector port of the switching-valve, located in the column manager.
  • Page 134 Remove the active preheater assembly from the retainer. Retainer Active preheater assembly While holding the column, disconnect the column outlet tubing. While holding the gold active preheater fitting, rotate the column off the fitting. If you are installing the active preheater in a system with Requirement: pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), use the 1/4-inch and 5/16-inch open-end wrenches to disconnect the compression fitting...
  • Page 135 Never connect active preheaters in the upper and lower Warning: positions at the same time. Doing so can damage the 30-cm column heater. Install the new active preheater assembly in the retainer. If you are replacing the active preheater in a 30-cm column heater: •...
  • Page 136 • Column manager – route the tubing between the column compartment and the side of the sample manager’s chassis, upward, to the switching valve. • Auxiliary column manager – route the tubing between the column compartment and the side of the instrument chassis to the switching valve.

  • Page 137: Replacing The Column Stabilizer Assembly (Ch-A Only)

    Replacing the column stabilizer assembly (CH-A only) To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment internal temperature to ensure all components are cool. To prevent electric shock, ensure all waste line tubing is Warning: properly connected, and the drip tray is fitted and undamaged.
  • Page 138 Disconnect the inlet tubing from injector port 6 of the sample manager. Location of column-stabilizer-inlet tubing Open the column heater door. Using the Phillips screwdriver, loosen the two screws that fasten the column stabilizer assembly to the column compartment tray. Remove the column stabilizer assembly from the thermal gasket.
  • Page 139 Column stabilizer assembly (two styles): For 150-mm column For 50-mm or 100-mm column Reusable fittings Inlet tubing to injector TP03123 Carefully route the column-stabilizer-inlet tubing through the groove on the side of the column compartment. To avoid stripping threads, do not overtighten screws. Caution: Insert the Phillips screws into the threaded holes, and tighten them gently using the Phillips screwdriver.

  • Page 140: Replacing The Frit In The In-Line Filter Unit

    11. Remove the O-ring retainer from the inlet end of the column stabilizer tubing, and then connect the inlet tubing to injector port 6 of the sample manager. Note: Do not route the tubing between the sample manager door and hinge, here. Column-stabilizer-inlet tubing 12.
  • Page 141 Stop any currently running mobile phase before replacing the Requirement: frit in the in-line filter unit. Required materials • Gloves: clean, powder-free, chemical-resistant • Replacement frit Required tools 5/16-inch open-end wrench (2) To replace the frit in the in-line filter unit: After allowing sufficient time for cooling, open the column heater, column manager, or auxiliary column manager door.
  • Page 142 Open the in-line filter unit by placing two 5/16-inch open-end wrenches onto the flats as shown. Flow Outlet assembly Frit Inlet nut TP02543 Place wrenches on flats and rotate to assemble and disassemble. Remove the in-line filter unit inlet nut, which contains the used frit. Tips: •...
  • Page 143 Replacing the column heater/cooler fuse (ACQUITY UPLC 30-cm CHC) To avoid damaging electrical components, replace fuses with Caution: those of the same type and rating. Column heater/cooler fuse ratings require: • Two fuses • 3.15 A, 250 V Fast-Blo, 5 × 20 mm (IEC)
  • Page 144 Insert the fuse holder into the receptacle, and gently push until it locks into position. A clicking sound indicates the fuse holder is locked into position. Tip: Connect the power cord to the rear panel power entry module. Cleaning the instrument’s exterior (all models) To prevent burn injuries, adjust the temperature set-point of Warning: the column manager to a safe temperature (10 to 30 °C) and allow the...

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