Installing the ESI small bore capillary option
The ESI small bore capillary option is for use with 1-mm UPLC columns
running at flowrates of 100 to 200 µL/min. The materials needed for this task
are in the Small-Bore Capillary kit.
Caution:
rates of 200 µL/min through the ESI probe when using the small-bore
capillary.
Required materials
•
Chemical-resistant, powder-free gloves
•
Combined 2.5-mm Allen wrench and cone extraction tool
•
10-mm wrench
•
8-mm wrench
•
2 × 7-mm wrenches
•
LC pump
•
HPLC-grade (or better) 1:1 acetonitrile:water
•
Sharp knife or PEEK tubing cutter
•
From the Small-Bore Capillary kit:
–
Capillary
–
Small-bore, UNF coupler (slide port)
–
Collar nut (thumb nut)
–
PTFE liner tube
–
Conductive sleeve
–
2 × 1/16-inch ferrules
•
Metal gasket for the probe tip
•
Safety goggles
3-8
Configuring the LockSpray Source
To avoid damage from excessive pressure, do not exceed flow