Page 2 March 14, 2014, 715004387 Rev. B...
Page 3: Trademarks
Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).
Page 4: Customer Comments
Contacting Waters ® Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information...
Page 5: Safety Hazard Symbol Notice
Warning: United States and HAR-type (or better) in Europe. The main power cord must only be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight.
Page 6 Spilled solvents hazard To avoid injury or equipment damage caused by spilled Prohibited: solvent, do not place reservoir bottles on top of the instrument or on its front ledge, unless in the bottle tray provided. Flammable solvents hazard To prevent ignition of flammable solvent vapors in the Warning: enclosed space of a mass spectrometer’s ion source, ensure that nitrogen flows continuously through the source.
Page 7 The source ion block, located behind the source enclosure assembly, can become hot. Xevo TQD high temperature hazard: Source enclosure assembly TP03406 March 14, 2014, 715004387 Rev. B...
Page 8 The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.
Page 9: Fcc Radiation Emissions Notice
FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
Page 10: Operating This Instrument
Operating this instrument When operating this instrument, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Date of manufacture Part number catalog number Serial number Supply ratings Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community...
Page 11 For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. March 14, 2014, 715004387 Rev. B...
Page 12: Audience And Purpose
TRIZAIC UPLC options, or optional third-party sources (DART , DESI, or LDTD™), the Xevo TQD does not comply with the European Union In Vitro Diagnostic Device Directive 98/79/EC. Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve.
Page 13: Emc Considerations
When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole blood, and other sources, note that the matrix components can adversely affect LC/MS results, enhancing or suppressing ionization. To minimize these matrix effects, adopt the following measures: •...
Page 14: Ec Authorized Representative
EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX United Kingdom Telephone: +44-161-946-2400 Fax: +44-161-946-2480 Contact: Quality manager March 14, 2014, 715004387 Rev. B...
Page 15: Table Of Contents
EC authorized representative ................. xiv 1 Specifications and Operating Modes ..........23 Uses and compatibility ..................24 ACQUITY Xevo TQD UPLC/MS systems............26 Non-ACQUITY devices for use with the Xevo TQD......... 29 Software and data system ................. 29 March 14, 2014, 715004387 Rev. B...
Page 16 Ionization techniques and source probes ............ 31 Electrospray ionization (ESI) ................31 Combined ESI and APCI (ESCi) ............... 31 Atmospheric pressure chemical ionization (APCI) .......... 31 Dual-mode APPI/APCI source................32 NanoFlow source....................32 Atmospheric solids analysis probe (ASAP)............33 Atmospheric pressure gas chromatography (APGC) ........33 TRIZAIC UPLC source ..................
Page 17 Purging the infusion pump................54 Rebooting the instrument ................54 Leaving the mass spectrometer ready for operation ........ 55 Emergency shutdown of the mass spectrometer..........55 3 Changing the Mode of Operation ............57 ESI mode ......................58 Installing the ESI probe ..................58 Removing the ESI probe..................
Page 18 4 Maintenance Procedures ..............81 Maintenance schedule ..................83 Spare parts ......................85 Troubleshooting with Connections INSIGHT ..........86 Safety and handling ..................87 Preparing the instrument for operations on or inside its source ..88 Removing and refitting the source enclosure ..........90 Removing the source enclosure from the instrument ........
Page 19 Fitting the sampling cone assembly to the source ......... 117 Cleaning the extraction cone ................ 119 Removing the ion block assembly from the source assembly ......119 Removing the extraction cone from the ion block .......... 121 Cleaning the extraction cone................123 Fitting the extraction cone to the ion block............
Page 20 Specific warnings ..................... 203 Notices ........................ 206 Prohibition symbol ..................206 Warnings that apply to all Waters instruments and devices ....207 Warnings that address the replacing of fuses ........... 212 Electrical and handling symbols ..............214 Electrical symbols .................... 214 Handling symbols ....................
Page 21 Connecting the Edwards oil-free roughing pump ........224 Connecting electric cables to the Edwards oil-free roughing pump....227 Connecting to the nitrogen gas supply ............228 Connecting to the collision cell gas supply ..........230 Connecting the nitrogen exhaust line ............230 Connecting the liquid waste line ..............
Page 22 xxii March 14, 2014, 715004387 Rev. B...
Page 23: Specifications And Operating Modes
Specifications and Operating Modes This chapter describes the instrument, including its controls and connections for gas and plumbing. Contents: Topic Page Uses and compatibility ..............24 Ionization techniques and source probes ........31 IntelliStart Fluidics system............. 34 Ion optics ..................36 MS operating modes ................
Page 24: Uses And Compatibility
Optional ASAP source For information on installing and removing the optional APGC, TRIZAIC, and ASAP sources, refer to the operator’s guide supplements supplied with them. You can also use the Xevo TQD with the following optional third-party sources: ® •...
Page 25 Uses and compatibility Xevo TQD shown with visor down, and visor up: Visor up TP03406 TP03407 IntelliStart technology IntelliStart™ technology monitors LC/MS/MS performance and reports when the instrument is ready for use. The software automatically tunes and mass calibrates the instrument, displays performance readbacks, and enables simplified setup of the system for use in routine analytical and open access applications.
Page 26: Acquity Xevo Tqd Uplc/Ms Systems
® The nanoACQUITY Xevo TQD UPLC/MS system includes a nanoACQUITY UPLC system and the Xevo TQD fitted with either a Nanoflow source, or a TRIZAIC source. If you are not using your instrument as part of an ACQUITY UPLC system, refer to the documentation for your LC system.
Page 27 ACQUITY UPLC H-Class System Guide, ACQUITY UPLC I-Class System Guide, and Controlling Contamination in UPLC/MS and HPLC/MS Systems (part number 715001307). You can find the documents on http://www.waters.com; click Services and Support > Support Library. March 14, 2014, 715004387 Rev. B...
Page 28 Xevo TQD with nanoACQUITY UPLC system The nanoACQUITY uses the optional NanoFlow source or TRIZAIC source, on the Xevo TQD. It is designed for capillary-to-nano-scale separations. Its sensitivity, resolution, and reproducibility well suit it for biomarker discovery and proteomics applications, including protein identification and characterization.
Page 29: Non-Acquity Devices For Use With The Xevo Tqd
Uses and compatibility Non-ACQUITY devices for use with the Xevo TQD The following non-ACQUITY LC devices are validated for use with the Xevo TQD: ® • Waters Alliance 2695 separations module • Waters Alliance 2795 separations module • Waters 2998 PDA detector •...
Page 30 1 Specifications and Operating Modes MassLynx v4.1 MassLynx software acquires, analyzes, manages, and distributes mass spectrometry, ultraviolet (UV), evaporative light scattering, and analog data. OpenLynx and TargetLynx application managers are included as standard software with MassLynx. See the MassLynx v4.1 user documentation and online Help for information about using MassLynx software.
Page 31: Ionization Techniques And Source Probes
Ionization techniques and source probes Available source options can vary depending on the software used to Note: operate the Xevo TQD. Refer to the MassLynx or UNIFI online Help for more information about supported sources. Electrospray ionization (ESI) In electrospray ionization (ESI), a strong electrical charge is applied to the eluent as it emerges from a nebulizer.
Page 32: Dual-Mode Appi/Apci Source
1 Specifications and Operating Modes Dual-mode APPI/APCI source The optional, combined atmospheric pressure photoionization/atmospheric pressure chemical ionization (APPI/APCI) source comprises an IonSABRE II probe and the APPI lamp drive assembly. The APPI lamp drive assembly comprises a UV lamp and a repeller electrode. In addition, a specially shaped, dual, APPI/APCI corona pin can be used.
Page 33: Atmospheric Solids Analysis Probe (Asap)
See the Atmospheric Solids Analysis Probe Operator’s Guide Supplement for further details. Atmospheric pressure gas chromatography (APGC) The Waters APGC couples an Agilent GC with the Xevo TQD, making it possible to perform LC and GC analyses in the same system, without compromising performance. The APGC provides complementary information to the LCMS instrument, enabling analysis of compounds of low molecular weight and/or low-to-intermediate polarity.
Page 34: Intellistart Fluidics System
1 Specifications and Operating Modes IntelliStart Fluidics system Functionality The IntelliStart Fluidics system is a solvent delivery system built into the mass spectrometer. It delivers sample or solvent directly to the MS probe in one of three ways: • From the LC column. •...
Page 35: System Operation
IntelliStart Fluidics system System operation The software automatically controls solvent and sample delivery during auto-tuning, auto-calibration, and method development. The selector valve systematically makes connections between the fluidics components to carry out the operations processed by the software. You can set IntelliStart fluidics configuration requirements in the system console.
Page 36: Ion Optics
1 Specifications and Operating Modes Ion optics The mass spectrometer’s ion optics operate as follows: Samples from the LC or Intellistart fluidics system are introduced at atmospheric pressure into the ionization source. The ions pass through the sample cone into the vacuum system. The ions pass through the transfer optics (the ion guide) to the first quadrupole, where they can be filtered according to their mass-to-charge ratios.
Page 37: Ms Operating Modes
MS operating modes MS operating modes The following table shows the MS operating modes. MS operating modes: Operating mode Collision cell Pass all masses Resolving (scanning) Pass all masses Resolving (static) In MS mode, the instrument can acquire data at scan speeds as high as 10,000 Da/s.
Page 38: Ms/Ms Operating Modes
1 Specifications and Operating Modes MS/MS operating modes The following table shows the MS/MS operating modes, described in more detail in the following pages. MS/MS operating modes: Operating mode Collision cell Product Static (at Fragment Scanning (daughter) ion precursor mass) precursor ions spectrum and pass all...
Page 39: Product (Daughter) Ion Mode
MS/MS operating modes Product (daughter) ion mode Product ion mode is the most commonly used MS/MS operating mode. You can specify an ion of interest for fragmentation in the collision cell, thus yielding structural information. Product ion mode: Collision cell Static (at precursor mass) Fragmenting Scanning...
Page 40: Precursor (Parent) Ion Mode
1 Specifications and Operating Modes Precursor (parent) ion mode Precursor ion mode: Collision cell Scanning Fragmenting Static (at product mass) precursor ions and passing all masses Typical application You typically use the precursor ion mode for structural elucidation—that is, to complement or confirm product scan data—by scanning for all the precursors of a common product ion.
Page 41 RADAR In RADAR mode the Xevo TQD rapidly alternates between MRM and full scan MS acquisition modes. The instrument tracks target analytes with precision in MRM mode, while at the same time scanning (in MS mode) the background for all other components.
Page 42: Constant Neutral Loss Mode
1 Specifications and Operating Modes Constant neutral loss mode Constant neutral loss mode detects the loss of a specific neutral fragment or functional group from an unspecified precursor(s). The scans of MS1 and MS2 are synchronized. When MS1 transmits a specific precursor ion, MS2 “looks”...
Page 43: Sample Inlet
Leak sensors Leak sensors in the Xevo TQD and the drip trays of the ACQUITY UPLC system continuously monitor system components for leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 mL of accumulated, leaked liquid in its surrounding reservoir.
Page 44: Rear Panel Connections
1 Specifications and Operating Modes Rear panel connections The following figure shows the rear panel locations of the connectors used to operate the instrument with external devices. Instrument rear panel: Event inputs and outputs Shielded Ethernet Roughing pump control Power Source vacuum Turbo vacuum Collision cell...
Page 45: Preparing For Operation
Preparing for Operation This chapter describes how to start and shut down the instrument. Contents: Topic Page Starting the mass spectrometer ............46 Preparing the IntelliStart Fluidics system........52 Rebooting the instrument..............54 Leaving the mass spectrometer ready for operation...... 55 March 14, 2014, 715004387 Rev.
Page 46: Starting The Mass Spectrometer
2 Preparing for Operation Starting the mass spectrometer The Waters Xevo TQD is compatible with the ACQUITY UPLC system; if you are not using an ACQUITY UPLC system, refer to the documentation relevant to the system you are using (see “Non-ACQUITY devices for use with Xevo TQD”...
Page 47 Starting the mass spectrometer Power-on the workstation, and log in. Press the power switch on the top, right-hand side of the mass spectrometer and the switches on the top, left-hand sides of the ACQUITY instruments. Each system instrument runs a series of startup tests. Result: Allow 3 minutes for the embedded PC (located inside the mass spectrometer) to initialize and to sound an alert indicating that the PC is...
Page 48 2 Preparing for Operation To evacuate (pump down) the mass spectrometer, follow the procedure below for MassLynx software or UNIFI software. MassLynx software: Click IntelliStart in the MassLynx main window’s lower left-hand corner. The mass spectrometer’s console appears. The mass Result: spectrometer is in Standby mode.
Page 49 Starting the mass spectrometer UNIFI software: From the Xevo TQD console, in the Maintain pane, click Vacuum. On the Status page, click Pump. After a 20-second delay, during which the turbopump is starting, Tip: the roughing pump starts. The Instrument System pane shows that the instrument is in Standby mode, and the Operate LED remains off.
Page 50: Verifying The Instrument's State Of Readiness
2 Preparing for Operation Verifying the instrument’s state of readiness When the instrument is in good operating condition, the power and Operate LEDs show constant green. You can view any error messages in IntelliStart software (MassLynx), or UNIFI software. Monitoring the instrument LEDs Light-emitting diodes on the instrument indicate its operational status.
Page 51: Running The Instrument At Different Flow Rates
Running the instrument at different flow rates The ACQUITY UPLC system runs at high flow rates. To optimize desolvation, and thus sensitivity, run the ACQUITY Xevo TQD system at appropriate gas flows and desolvation temperatures. IntelliStart software automatically sets these parameters when you enter a flow rate, according to the following table.
Page 52: Preparing The Intellistart Fluidics System
2 Preparing for Operation Preparing the IntelliStart Fluidics system For additional information, see page 233 Appendix To avoid damaging the mass spectrometer by accidentally Notice: spilling solvent on it, do not store large-volume solvent reservoirs on top of the instrument. Installing the reservoir bottles Use standard reservoir bottles (15-mL) for instrument setup and calibration.
Page 53 Preparing the IntelliStart Fluidics system To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while installing reservoir bottles. To install the reservoir bottles: Remove the reservoir bottle caps. Screw the reservoir bottles onto the instrument, as shown below.
Page 54: Purging The Infusion Pump
2 Preparing for Operation Purging the infusion pump Whenever you replace a solution bottle, purge the infusion pump with the solution that you are going to use next. See the mass spectrometer’s online Help for details. Ensure that the end of the tubing is fully submerged in the Requirement: solvent in the wash reservoir.
Page 55: Leaving The Mass Spectrometer Ready For Operation
Leaving the mass spectrometer ready for operation Remove the PEEK tubing from the reset button aperture. Wait until the reboot sequence ends before starting the mass spectrometer software. An audible alert sounds when the reboot sequence is complete. Tip: Leaving the mass spectrometer ready for operation Leave the mass spectrometer in Operate mode except in the following cases: •...
Page 56 2 Preparing for Operation March 14, 2014, 715004387 Rev. B...
Page 57: Changing The Mode Of Operation
For details about other Waters and third-party source options, refer to the documentation supplied with the source. Available source options can vary depending on the software used Note: to operate the Xevo TQD. Refer to the MassLynx or UNIFI online Help for more information about supported sources. Contents: Topic Page ESI mode ..................
Page 58: Esi Mode
3 Changing the Mode of Operation ESI mode The following sections explain how to install and remove an ESI probe. For further details on running ESI applications, see page Installing the ESI probe Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant,...
Page 59 ESI mode With the probe label facing you, carefully slide the ESI probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with the location hole of the probe adjuster assembly. Probe label Probe locking ring Probe location dowel Location hole of the probe adjuster assembly...
Page 60 3 Changing the Mode of Operation To avoid electric shock, do not use stainless steel tubing Warning: or stainless steel “finger tight” screws to connect the selector valve to the ESI probe; use the PEEK tubing and natural (beige) colored PEEK “finger tight”...
Page 61: Removing The Esi Probe
ESI mode Removing the ESI probe Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while performing this procedure. The LC system connections, ESI probe, and source can be contaminated.
Page 62: Esci Mode
3 Changing the Mode of Operation ESCi mode To run ESCi applications, you must fit an ESI probe and corona pin to the ESI/APCI/ESCi source enclosure. See “Installing the ESI probe” on page 58, “Installing the corona pin in the source”...
Page 63: Installing The Ionsabre Ii Probe
APCI mode discharge to produce stable reagent ions. Analyte molecules introduced into the mobile phase react with the reagent ions at atmospheric pressure and typically become protonated (in the positive ion mode) or deprotonated (in the negative ion mode). The sample and reagent ions then pass through the sample cone and into the mass spectrometer.
Page 64 3 Changing the Mode of Operation Probe label Probe locking ring Probe location dowel Location hole of the probe adjuster assembly TP03129 Tighten the probe locking ring to secure the probe in place. Fully tighten the probe locking ring. Doing so ensures a Requirement: successful result on the automatic pressure test, which runs when the probe is correctly seated, and the source enclosure door is closed.
Page 65: Removing The Ionsabre Ii Probe
APCI mode Removing the IonSABRE II probe Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while performing this procedure. The LC system connections, IonSABRE II probe, and source can be contaminated.
Page 66: Combined Appi/Apci Source
3 Changing the Mode of Operation Combined APPI/APCI source Operate this optional, replacement source enclosure in APPI, APCI, or dual APPI/APCI mode. Dual-mode APPI/APCI performs rapid switching between ionization modes. APPI operation In atmospheric pressure photoionization (APPI) mode, the source is fitted with an IonSABRE II probe and the APPI lamp drive assembly is advanced into the source.
Page 67: Apci Operation
Combined APPI/APCI source APCI operation The atmospheric pressure chemical ionization (APCI) mode produces singly charged protonated or deprotonated molecules for a large range of nonvolatile analytes. In APCI mode, the source is fitted with an APCI corona pin. Unused, the APPI lamp drive assembly is retracted from the source. APCI mode: IonSABRE II probe Sample cone...
Page 68: Dual-Mode Operation
3 Changing the Mode of Operation Dual-mode operation Dual-mode operation enables rapid switching between APPI and APCI ionization modes and allows high-throughput operations (for example, for sample screening). You replace the standard corona pin with a specially shaped APPI/APCI corona pin, so that the APPI lamp holder can be advanced into the source for dual operation.
Page 69: The Combined Appi/Apci Source Components
Combined APPI/APCI source The combined APPI/APCI source components The combined APPI/APCI source comprises the standard IonSABRE II probe and a source enclosure with an APPI lamp drive incorporated. The combined APPI/APCI source enclosure: APPI lamp drive assembly To prevent damage to the corona pin and lamp assembly, ensure Notice: that the lamp assembly does not touch the corona pin when the source enclosure door is closed.
Page 70 3 Changing the Mode of Operation The UV lamp, which you ignite via a control in the MassLynx Tune window, provides a constant photon output. You vary the intensity of incident radiation upon the sample molecules by adjusting the distance between the UV lamp and probe tip.
Page 71: Installing The Combined Appi/Apci Source
Combined APPI/APCI source Installing the combined APPI/APCI source Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while performing this procedure. The source components can be contaminated.
Page 72: Removing The Ionsabre Ii Probe And Appi/Apci Source Enclosure
3 Changing the Mode of Operation Connect the HT cable to the instrument’s front panel connector. To prevent damage to the corona pin and lamp assembly, Notice: ensure that the lamp assembly does not touch the corona pin when the source enclosure door is closed. Install the IonSABRE II probe to the source, and ensure that it is working correctly (see page...
Page 73: Nanoflow Source
NanoFlow source Remove the corona pin (see page 95). Fit the blanking plug to the pin’s mounting contact. NanoFlow source The NanoFlow source enclosure comprises the NanoFlow stage (for x-axis, y-axis, and z-axis adjustment), the sprayer-enclosure, and a microscope camera. NanoFlow source, stage and microscope camera: Microscope Camera Sprayer enclosure...
Page 74: Installing The Nanoflow Source
3 Changing the Mode of Operation A light within the source provides illumination for the spray, which you can observe using the microscope camera mounted on the corner of the source housing. The low flow rates involved with operating the NanoFlow source prohibit its use with the instrument’s solvent delivery system.
Page 75 (mounted beneath the stage on the front of the NanoFlow source) and your sprayer. For details regarding how to fit each sprayer, see the corresponding Tip: reference: • Waters Universal NanoFlow Sprayer Installation and Maintenance Guide (part number 71500110107) • “Fitting a borosilicate glass capillary (nanovial)” on page 77 •...
Page 76 3 Changing the Mode of Operation Connect the probe cable to the high-voltage connector. Connect the high-voltage cable to the instrument’s HV connection. High-voltage cable The NanoFlow stage contains a high-voltage interlock, so the Tip: capillary voltage (the voltage applied to the sprayer assembly) and the sampling cone voltage remain disabled until the sprayer is pushed fully forward in the source.
Page 77: Fitting A Borosilicate Glass Capillary (Nanovial)
NanoFlow source Fitting a borosilicate glass capillary (nanovial) Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • Borosilicate glass capillary ® • Fused silica syringe needle or GELoader • Fused silica cutter To avoid lacerations, puncture injuries, and Warning: possible contamination with biohazardous and toxic samples, do not touch the sharp end of the capillary.
Page 78 3 Changing the Mode of Operation Unscrew the union from the end of the sprayer assembly. Capillary Union Remove the existing capillary from the sprayer. Carefully remove the new borosilicate glass capillary from its case by lifting vertically while pressing on the foam with two fingers. Foam Capillary Load sample into the capillary using a fused silica syringe needle or a...
Page 79 NanoFlow source When using a GELoader tip, break the glass Recommendation: capillary in half, scoring it with a fused silica cutter so that the GELoader can reach the capillary’s tip. Thread the knurled nut and approximately 5 mm of conductive elastomer over the blunt end of the capillary.
Page 80: Positioning The Borosilicate Glass Capillary Tip
3 Changing the Mode of Operation To prevent the capillary tip from colliding with the cone or Notice: the side of the source, adjust the sprayer tip position before you push the sprayer inside the NanoFlow source enclosure. 15. Carefully push the stage back into the NanoFlow source enclosure, using the stop and handle.
Page 81: Maintenance Procedures
Maintenance Procedures This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents: Topic Page Maintenance schedule ..............83 Spare parts ..................85 Troubleshooting with Connections INSIGHT ........
Page 82 4 Maintenance Procedures Contents: Topic Page Cleaning the IonSABRE II probe tip ..........159 Replacing the IonSABRE II probe sample capillary ...... 160 Cleaning or replacing the corona pin ..........166 Replacing the IonSABRE II probe heater........167 Replacing the ion block source heater..........170 Replacing the source assembly seals ..........
Page 83: Maintenance Schedule
Maintenance schedule Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance. The maintenance frequencies shown apply to instruments that normally receive moderate use. Maintenance schedule: Procedure Frequency For information... Clean the instrument case. As required. page 100.
Page 84 4 Maintenance Procedures Maintenance schedule: Procedure Frequency For information... Clean the IonSABRE II probe When sensitivity page 159. tip. (Options using the decreases to IonSABRE II probe only.) unacceptable levels or when significant chemical interference is present. Replace the IonSABRE II When sensitivity page 160.
Page 85: Spare Parts
Spare parts Replace only spare parts, which are the parts mentioned in this document. For ® details about spare parts, use the Waters Quality Parts Locator on the Waters Web site’s Services & Support page. March 14, 2014, 715004387 Rev. B...
Page 86: Troubleshooting With Connections Insight
Connections INSIGHT is an “intelligent” device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system. To use Connections INSIGHT, you must install its service agent software on your workstation. In a client/server system, the service agent must also be installed on the computer from which you control the system.
Page 87: Safety And Handling
Safety and handling Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: To avoid personal contamination, always wear Warning: chemical-resistant, powder-free gloves while handling instrument components. The components can be contaminated with biohazards or toxic materials. To prevent injury, always observe Good Laboratory Warning: Practice when handling solvents, changing tubing, or operating...
Page 88: Preparing The Instrument For Operations On Or Inside Its Source
In the Instrument Console, click Stop Flow to stop the LC flow or, if column flow is required, divert the LC flow to waste as follows: In the Instrument Console system tree, expand Xevo TQD, Interactive Fluidics. Click Control Select Waste as the flow state.
Page 89 Preparing the instrument for operations on or inside its source To use UNIFI software to prepare the instrument for operations on or inside its source: On the System Console tool bar, click Stop Flow to stop the LC flow or, if column flow is required, divert the LC flow to waste as follows: On the Setup pane, click Fluidics.
Page 90: Removing And Refitting The Source Enclosure
4 Maintenance Procedures Removing and refitting the source enclosure The optional combined APPI/APCI, NanoFlow, and TRIZAIC sources are supplied as a complete source enclosure. To fit them, you must first remove the standard source enclosure. Removing the source enclosure from the instrument Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazardous...
Page 91 Removing and refitting the source enclosure Using both hands, grasp the source enclosure and lift it vertically off the two supporting studs on the source adaptor housing. Cable storage positions Supporting stud TP03164 Source enclosure Store the cables neatly by plugging them into the cable-storage positions on the rear of the source enclosure.
Page 92: Fitting The Source Enclosure To The Instrument
4 Maintenance Procedures Fitting the source enclosure to the instrument Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biologically Warning: hazardous, or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.
Page 93: Installing And Removing The Corona Pin
Installing and removing the corona pin Installing and removing the corona pin For ESCi, APCI, and dual-mode APCI/APPI operation, you must fit a corona pin. Installing the corona pin in the source Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biologically Warning: hazardous, or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean,...
Page 94 4 Maintenance Procedures Remove the blanking plug from the corona pin mounting contact. Store the blanking plug in a safe location. Tip: Corona pin mounting contact: Corona pin mounting contact blanking plug TP03130 To avoid puncture wounds, handle the corona pin with Warning: care;...
Page 95: Removing The Corona Pin From The Source
Installing and removing the corona pin Close the source enclosure. Look through the source window, and use the vernier probe adjuster to position the probe tip so that it is pointing approximately midway between the tips of the sample cone and corona pin. Removing the corona pin from the source Required material Chemical-resistant, powder-free gloves...
Page 96: Operating The Source Isolation Valve
4 Maintenance Procedures Operating the source isolation valve You must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant,...
Page 97 Operating the source isolation valve Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Isolation valve handle in closed position To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.
Page 98 4 Maintenance Procedures Isolation valve handle in open position TP03130 Close the source enclosure. March 14, 2014, 715004387 Rev. B...
Page 99: Removing O-Rings And Seals
Removing O-rings and seals Removing O-rings and seals When performing certain maintenance procedures, you must remove O-rings or seals from instrument components. An O-ring removal kit can be purchased separately. O-ring removal kit: Tool 1 Tool 2 To avoid scratching the O-ring or seal, take care while using the Notice: removal tools to remove it from components.
Page 100: Cleaning The Instrument Case
4 Maintenance Procedures Cleaning the instrument case To avoid damaging the instrument, do not use abrasives or Notice: solvents to clean the instrument’s case. Use a soft cloth, dampened with water, to clean the outside surfaces of the instrument. Emptying the exhaust trap bottle Inspect the exhaust trap bottle in the instrument’s exhaust line daily, and empty it before it is more than 10% full.
Page 101 Emptying the exhaust trap bottle Required material Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: To stop the LC flow, follow the action below for your software: Software Action MassLynx In the instrument console, click Stop Flow UNIFI On the System Console tool bar, click Stop Flow Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure.
Page 102: Gas Ballasting The Roughing Pump
4 Maintenance Procedures To start the LC flow, follow the action below for your software: Software Action MassLynx In the instrument console, click Start Flow UNIFI On the System Console tool bar, click Start Flow Gas ballasting the roughing pump The roughing pump draws large quantities of solvent vapors.
Page 103: Gas Ballasting A Pump Fitted With A Screwdriver-Operated Gas Ballast Valve
Gas ballasting the roughing pump To avoid shortening the oil life and pump life, routinely gas Notice: ballast the roughing pump according to the guidelines below. Gas ballast the roughing pump when these conditions apply: • With ESI operation, once a week •...
Page 104 4 Maintenance Procedures To gas ballast the roughing pump: Use the flat-blade screwdriver to turn the gas ballast valve on the pump a quarter-turn to the open position TP02654 Run the pump for 30 to 60 minutes. It is normal for the roughing pump temperature to increase during Tip: ballasting.
Page 105: Gas Ballasting A Pump Fitted With A Handle-Operated Gas Ballast Valve
Gas ballasting the roughing pump Gas ballasting a pump fitted with a handle-operated gas ballast valve To avoid burn injuries, take great care while working with the Warning: roughing pump; it can be hot. To avoid damage, follow these guidelines: Notice: •...
Page 106: Checking The Roughing Pump Oil Level
4 Maintenance Procedures Checking the roughing pump oil level To ensure correct operation of the roughing pump, do not operate it with the oil level at less than 30% of the maximum level, as indicated in the pump’s sight glass. This procedure is not required for an Edwards oil-free roughing pump.
Page 107 Adding oil to the roughing pump To add oil to the roughing pump: Vent and shut down the mass spectrometer (see the instrument’s online Help for details). Use the 8-mm Allen wrench to unscrew and remove the roughing pump’s oil filler plug (see the figure on page 102).
Page 108: Cleaning The Source Components
4 Maintenance Procedures Cleaning the source components Clean the sample cone and cone gas nozzle when these conditions apply: • The sample cone and cone gas nozzle are visibly fouled. • You dismissed LC and sample-related causes for decreased signal intensity.
Page 109: Cleaning The Sampling Cone Assembly
Cleaning the sampling cone assembly Cleaning the sampling cone assembly You can remove the sampling cone assembly (the sample cone, O-ring, and cone gas nozzle) for cleaning without venting the instrument. Removing the sampling cone assembly from the source Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning:...
Page 110 4 Maintenance Procedures To remove the sampling cone assembly from the source: Close the source isolation valve (see page 96). Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly 90 degrees, moving the handle from the vertical to the horizontal position.
Page 111: Disassembling The Sampling Cone Assembly
Cleaning the sampling cone assembly Disassembling the sampling cone assembly Required material • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the sampling cone assembly.
Page 112 4 Maintenance Procedures Insert the collar in the sample cone. To avoid damaging the fragile sample cone, do not place Notice: it on its tip; instead, place it on its flanged base. Rotate the tool and collar counter-clockwise and then lift them to remove the sample cone from the cone gas nozzle.
Page 113 Cleaning the sampling cone assembly Remove the O-ring from the sample cone. Cone gas nozzle Cone gas nozzle handle O-ring To avoid spreading contamination, dispose of the Warning: O-ring or seal in accordance with local environmental regulations. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.
Page 114: Cleaning The Sample Cone And Cone Gas Nozzle
4 Maintenance Procedures Cleaning the sample cone and cone gas nozzle Required materials • Chemical-resistant, powder-free gloves. • Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol. •...
Page 115 Cleaning the sampling cone assembly Place the vessels in the ultrasonic bath for 30 minutes. If you used formic acid in the cleaning solution, do as follows: Rinse the components by immersing them in separate glass vessels containing water and then place the vessels in the ultrasonic bath for 20 minutes.
Page 116: Assembling The Sampling Cone Assembly
4 Maintenance Procedures Assembling the sampling cone assembly Required material Clean, chemical-resistant, powder-free gloves Notice: • To avoid recontaminating the sampling cone assembly, wear clean chemical-resistant, powder-free gloves during this procedure. • To avoid damaging the fragile sample cone, do not place it on its tip; instead, place it on its flanged base.
Page 117: Fitting The Sampling Cone Assembly To The Source
Cleaning the sampling cone assembly Fitting the sampling cone assembly to the source Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components. To avoid puncture wounds, take great care while Warning: working with the source enclosure open if one or both of these...
Page 118 4 Maintenance Procedures To fit the sampling cone assembly to the source: Ensure that the source isolation valve is in the closed position (see page 96). Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly.
Page 119: Cleaning The Extraction Cone
Cleaning the extraction cone Cleaning the extraction cone Clean the ion block and extraction cone when cleaning the sample cone and cone gas nozzle fails to increase signal sensitivity. You must remove the ion block assembly from the source assembly to clean the extraction cone. Removing the ion block assembly from the source assembly Required materials •...
Page 120 4 Maintenance Procedures Use the combined 2.5-mm Allen wrench and cone extraction tool to unscrew the 4 captive screws that secure the ion block assembly. Screws securing the Ion block TP03130 Remove the ion block assembly from the PEEK ion block support. PEEK ion block support Ion block assembly...
Page 121: Removing The Extraction Cone From The Ion Block
Cleaning the extraction cone Removing the extraction cone from the ion block Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the ion block components.
Page 122 4 Maintenance Procedures Notice: • To avoid damaging the extraction cone aperture, take great care when removing it from the ion block. • To avoid damaging the fragile extraction cone, do not place it on its tip; instead, place it on its flanged base. Remove the extraction cone from the ion block.
Page 123: Cleaning The Extraction Cone
Cleaning the extraction cone Cleaning the extraction cone Required materials • Chemical-resistant, powder-free gloves. • Appropriately sized glass vessel in which to completely immerse the extraction cone when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol. •...
Page 124 4 Maintenance Procedures To clean the extraction cone: Immerse the extraction cone in the glass vessel containing 1:1 methanol/water. If the extraction cone is obviously contaminated, use 45:45:10 Tip: methanol/water/formic acid. Place the vessel in the ultrasonic bath for 30 minutes. If you used formic acid in the cleaning solution, do as follows: Rinse the extraction cone by immersing it in a glass vessel containing water and then placing the vessel in the ultrasonic bath...
Page 125: Fitting The Extraction Cone To The Ion Block
Cleaning the extraction cone Fitting the extraction cone to the ion block Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the ion block.
Page 126: Cleaning The Ion Block Assembly
4 Maintenance Procedures To avoid recontaminating the ion block assembly, wear Notice: clean chemical-resistant, powder-free gloves during this procedure. To fit the ion block assembly to the source assembly: Fit the ion block assembly to the PEEK ion block support. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and then slowly tighten the 4 ion block assembly securing screws sequentially and in small increments.
Page 127 Cleaning the ion block assembly Ensure that the isolation valve is closed. Isolation valve handle in closed position Sampling cone assembly retaining blocks Cone gas nozzle handle Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly through 90 degrees.
Page 128 4 Maintenance Procedures Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 ion block cover plate captive securing screws. Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Grasp the isolation valve and pull it out of the ion block.
Page 129 Cleaning the ion block assembly Use the O-ring removal kit to carefully remove the isolation valve O-ring (see page 99). To avoid spreading contamination, dispose of the Warning: isolation valve O-ring in accordance with local environmental regulations. If the isolation valve O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.
Page 130 4 Maintenance Procedures 12. Holding the PEEK ion block gently, use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and carefully slide it and the PEEK terminal block out of the ion block. Heat-shrink tubing PEEK terminal block Heater cartridge assembly 13.
Page 131 Cleaning the ion block assembly 14. Use the O-ring removal kit to carefully remove the cone gas O-ring from the ion block. To avoid spreading contamination, dispose of the Warning: cover seal and cone gas O-ring in accordance with local environmental regulations.
Page 132 4 Maintenance Procedures 18. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the captive screws securing the 2 PEEK extraction cone retainer clips. Securing screw Retainer clip Extraction cone Notice: • To avoid damaging the extraction cone aperture, take great care when removing the extraction cone from the ion block.
Page 133: Cleaning The Ion Block Components
Cleaning the ion block assembly 20. Remove the extraction cone handle insulator from the extraction cone handle. 21. Remove the extraction cone seal from the ion block. Extraction cone seal Cleaning the ion block components Required materials • Chemical-resistant, powder-free gloves. •...
Page 134 4 Maintenance Procedures To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the ion block components. To avoid injury, work with extreme care. Use a fume Warning: hood, and suitable protective equipment.
Page 135: Assembling The Source Ion Block Assembly
Cleaning the ion block assembly To avoid spreading contamination, dispose of the Warning: components in accordance with local environmental regulations. Inspect each component for persisting contamination. If contamination is present, dispose of the component, and Requirement: obtain a new one before reassembling the ion block. Assembling the source ion block assembly Required materials •...
Page 136 4 Maintenance Procedures Use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the captive screw securing each extraction cone retainer clip to the ion block. Fit the blanking plug seal onto the ion block blanking plug. Fit a new seal if the old one was discarded. Requirement: Fit the blanking plug onto the ion block and finger tighten it.
Page 137: Cleaning The Ion Guide Assembly
Cleaning the ion guide assembly 16. Fit the isolation valve onto the ion block assembly, so that it is in the closed position. 17. Fit the ion block cover plate onto the ion block assembly, and then use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the 2 captive securing screws on the ion block’s cover plate.
Page 138 4 Maintenance Procedures Remove the ion block assembly from the PEEK ion block support (see page 119). Use the 3-mm Allen wrench to unscrew and remove the 4 screws securing the PEEK ion block support to the adaptor housing, and remove the ion block support.
Page 139 Cleaning the ion guide assembly To avoid damaging the ion guide assembly when removing Notice: it from the source assembly, do not grasp the ion guide by its metal lens plates. Instead, grasp the circuit boards on the top and bottom of the device.
Page 140: Cleaning The Ion Guide Assembly
4 Maintenance Procedures Cleaning the ion guide assembly Required materials • Chemical-resistant, powder-free gloves. • Glass-fiber pen. • 500-mL measuring cylinder or appropriately sized glass vessel in which to completely immerse the ion guide when cleaning. Use only glassware not previously cleaned with surfactants. •...
Page 141 Cleaning the ion guide assembly To avoid vibration-caused damage to the ion guide Notice: assembly, ensure that the bottom of the assembly is not in contact with the bottom of the glass vessel. Use the hook to carefully suspend the ion guide assembly in the glass vessel so that the bottom of the assembly does not touch the bottom of the vessel.
Page 142: Removing The Differential Aperture From The Ion Guide
4 Maintenance Procedures Removing the differential aperture from the ion guide Required material Small, flat-blade screwdriver To avoid a reduction in sensitivity, remove and clean the Notice: differential aperture only when cleaning the ion guide assembly fails to remove all visible contamination from the differential aperture.
Page 143 Cleaning the ion guide assembly To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when handling the differential pumping aperture, and throughout this procedure. To clean the differential aperture: Use the glass-fiber pen to remove gross contamination from the differential aperture by gentle abrasion.
Page 144: Fitting The Differential Aperture To The Ion Guide Assembly
4 Maintenance Procedures Fitting the differential aperture to the ion guide assembly Required materials • Clean, chemical-resistant, powder-free gloves • Small, flat-blade screwdriver To fit the differential aperture onto the ion guide assembly: Hold the differential aperture against the support rods so that the rods align with the 3 holes in the differential aperture’s base.
Page 145: Fitting The Ion Guide Assembly To The Source Assembly
Cleaning the ion guide assembly Fitting the ion guide assembly to the source assembly Required material Clean, chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.
Page 146 4 Maintenance Procedures If contamination is present, use 1:1 methanol/water, applied to a Tip: lint-free cloth, to carefully clean the grooves. Fit the O-rings (new ones if you disposed of the old) to the PEEK ion block support. To fit an O-ring in its groove, start fitting the O-ring at the notch in Tip: the groove, and then progressively work the ring into the groove, in either direction from the notch.
Page 147: Replacing The Esi Probe Tip And Gasket
Replacing the ESI probe tip and gasket Replacing the ESI probe tip and gasket Replace the ESI probe tip if a blockage occurs in the internal metal sheathing through which the stainless steel capillary passes or if the probe tip is damaged.
Page 148 4 Maintenance Procedures Use the 10-mm wrench to remove the probe tip. 10-mm wrench Probe tip If the probe tip is difficult to remove, use the 7-mm wrench in Tip: conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip March 14, 2014, 715004387 Rev.
Page 149 Replacing the ESI probe tip and gasket Remove the metal gasket from the probe tip. Metal gasket To avoid spreading contamination, dispose of the Warning: probe tip and metal gasket in accordance with local environmental regulations. They can be contaminated with biohazards or toxic materials.
Page 150: Fitting The Esi Probe Tip And Gasket
4 Maintenance Procedures Fitting the ESI probe tip and gasket Required materials • Chemical-resistant, powder-free gloves • 7-mm wrench • New metal gasket To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components.
Page 151: Replacing The Esi Probe Sample Capillary
Replacing the ESI probe sample capillary Replacing the ESI probe sample capillary Replace the stainless steel sample capillary in the ESI probe if it becomes blocked and cannot be cleared, or if it becomes contaminated or damaged. Removing the existing capillary Required materials •...
Page 152 4 Maintenance Procedures Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 screws retaining probe end-cover. End-cover retaining screws Remove the end cover and gasket from the probe assembly. Nebulizer adjuster knob Gasket End cover Unscrew and remove the nebulizer adjuster knob.
Page 153 Replacing the ESI probe sample capillary Use the 10-mm wrench to remove the probe tip. 10-mm wrench Probe tip If the probe tip is difficult to remove, use the 7-mm wrench in Tip: conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip March 14, 2014, 715004387 Rev.
Page 154 4 Maintenance Procedures Remove the metal gasket from the probe tip. Metal gasket Remove the PEEK union/UNF coupling assembly and capillary from the probe. PEEK union/UNF coupling assembly Capillary Unscrew and remove the knurled collar from the UNF coupling. PEEK union UNF coupling Locknut Knurled collar...
Page 155: Installing The New Capillary
Replacing the ESI probe sample capillary 12. Unscrew the finger-tight PEEK union from the UNF coupling. Ferrule PTFE liner sleeve 13. Remove the ferrule and PTFE liner sleeve from the capillary. 14. Remove the capillary from the UNF coupling. To avoid spreading contamination, dispose of the Warning: capillary, PTFE liner sleeve, and ferrule in accordance with local environmental regulations.
Page 156 4 Maintenance Procedures To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. To avoid puncture wounds, handle the probe with care; Warning: the ESI probe tip is sharp.
Page 157 Replacing the ESI probe sample capillary Use the needle-nose pliers to slide a new liner sleeve and ferrule onto the capillary. Insert the capillary in the PEEK union, and ensure that it is fully seated. Screw the UNF coupling into the PEEK union, finger-tight only. Gently tug on the capillary, testing to ensure that it stays in place.
Page 158 4 Maintenance Procedures 15. Carefully push the PEEK union/UNF coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. UNF coupling locating pin Probe assembly locating slot 16.
Page 159: Cleaning The Ionsabre Ii Probe Tip
See the mass spectrometer’s online Help for further details. To clean the IonSABRE II probe tip: On the Instrument Console system tree, click Xevo TQD > Manual optimization. On the Manual Optimization page, click to stop the liquid flow.
Page 160: Replacing The Ionsabre Ii Probe Sample Capillary
4 Maintenance Procedures Replacing the IonSABRE II probe sample capillary Replace the stainless steel sample capillary in the IonSABRE II probe if it becomes blocked and you cannot clear it, or if it becomes contaminated or damaged. Removing the existing capillary Required materials •...
Page 161 Replacing the IonSABRE II probe sample capillary Remove the end cover and gasket. Nebulizer adjuster knob Gasket End-cover Unscrew and remove the nebulizer adjuster knob. Remove the PEEK union/UNF coupling assembly and capillary from the probe. The PEEK union used with the IonSABRE II probe is notched on Tip: one of its flats, a feature that distinguishes it from the PEEK union used with the ESI probe (see “Replacing the ESI probe sample capillary”...
Page 162: Installing The New Capillary
4 Maintenance Procedures Use the 7-mm wrench to loosen the locknut. Unscrew the finger-tight PEEK union from the UNF coupling. Ferrule Remove the ferrule from the capillary. 10. Remove the capillary from the UNF coupling. To avoid spreading contamination, dispose of the Warning: capillary and ferrule in accordance with local environmental regulations.
Page 163 Replacing the IonSABRE II probe sample capillary To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. To install the new capillary: Use the sharp knife or PEEK tubing cutter to cut a piece of red PEEK tubing approximately 60 cm (24 inches) long.
Page 164 4 Maintenance Procedures Use the needle-nose pliers to slide a new ferrule onto the capillary. Insert the capillary in the PEEK union, and ensure that it is fully seated. Screw the UNF coupling into the PEEK union, finger-tight only. Gently tug on the capillary, testing to ensure that it stays in place. Use the 7-mm wrench to tighten the locknut against the PEEK union.
Page 165 Replacing the IonSABRE II probe sample capillary 15. Carefully push the PEEK union/UNF coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. Probe assembly locating slot UNF coupling locating pin 16.
Page 166: Cleaning Or Replacing The Corona Pin
4 Maintenance Procedures Cleaning or replacing the corona pin Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • HPLC-grade (or better) methanol • Lint-free tissue • Lapping film • Corona pin To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source...
Page 167: Replacing The Ionsabre Ii Probe Heater
Replacing the IonSABRE II probe heater Replacing the IonSABRE II probe heater Replace the IonSABRE II probe heater if it fails to heat the probe. Removing the IonSABRE II probe heater Required material Chemical-resistant, powder-free gloves To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant,...
Page 168 4 Maintenance Procedures To avoid damaging the probe heater’s electrical Notice: connections, do not twist the heater when removing it from the probe assembly. Gripping the probe heater as shown, carefully pull it off the probe assembly. Probe heater To avoid spreading contamination, dispose of the Warning: probe heater in accordance with local environmental regulations.
Page 169: Fitting The New Ionsabre Ii Probe Heater
Replacing the IonSABRE II probe heater Fitting the new IonSABRE II probe heater Required materials • Chemical-resistant, powder-free gloves • IonSABRE II probe heater To avoid damaging the probe heater’s electrical Notice: connections, capillary sleeve, or capillary, take great care when fitting the heater over the capillary sleeve.
Page 170: Replacing The Ion Block Source Heater
4 Maintenance Procedures Replacing the ion block source heater Replace the ion block source heater if it fails to heat when the instrument is pumped down (evacuated). Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • Combined 2.5-mm Allen wrench and cone extraction tool •...
Page 171 Replacing the ion block source heater Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. March 14, 2014, 715004387 Rev.
Page 172 4 Maintenance Procedures Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 2 screws securing the heater wires to the PEEK terminal block. Heater cartridge wire securing screws PEEK terminal block Use the needle-nose pliers to carefully swing the ring terminal tags out of the terminal block.
Page 173 Replacing the ion block source heater Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly and slide the assembly out of the ion block. Heat-shrink tubing Heater cartridge assembly Dispose of the heater cartridge assembly. To avoid damaging the heater cartridge assembly wires, Notice: do not bend or twist them when fitting the assembly to the ion...
Page 174: Replacing The Source Assembly Seals
4 Maintenance Procedures Replacing the source assembly seals To avoid solvent vapors of biohazards and toxic Warning: materials leaking into the laboratory atmosphere, replace the seals listed below exactly as described in this section. To avoid solvent vapors of biohazards and toxic Warning: materials leaking into the laboratory atmosphere, replace the seals listed below at intervals not exceeding one year.
Page 175 Replacing the source assembly seals To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components. To remove the probe adjuster assembly probe and source enclosure seals: Remove the source enclosure from the instrument (see page 90).
Page 176: Fitting The New Source Enclosure And Probe Adjuster Assembly Seals
4 Maintenance Procedures Nebulizer gas seal Desolvation gas seal Source enclosure seal TP03164 To avoid spreading contamination, dispose of the Warning: seals in accordance with local environmental regulations. The seals can be contaminated with biohazardous or toxic materials. Dispose of all the seals in accordance with local environmental regulations.
Page 177 Replacing the source assembly seals To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components. To fit the new source enclosure and probe adjuster assembly probe seals: Ensure that all the grooves for seals are free from dirt and debris.
Page 178: Replacing The Air Filter
4 Maintenance Procedures Replacing the air filter You must replace the air filter annually. Required material New air filter To replace the air filter: Lift the instrument’s visor so that the source probe is fully exposed. Fully open the source enclosure. Disconnect the probe cable from the high-voltage connector, and leave the cable in a position that does not obstruct the air filter grill.
Page 179 Replacing the air filter Open the air filter grill by pulling the tab at the top of the grill toward you. Air filter grill tab Air filter grill TP03405 Remove and dispose of the old filter. Place the new filter flat on the inside part of the grill, with its edges beneath the metal lip.
Page 180: Replacing The Roughing Pump Oil
4 Maintenance Procedures Replacing the roughing pump oil Change the roughing pump oil annually. This procedure is not required for an Edwards oil-free roughing pump. Note: Required materials • Chemical-resistant, powder-free gloves • 8-mm Allen wrench • Flat-blade screwdriver • Container to catch used oil •...
Page 181 Replacing the roughing pump oil Use the 8-mm Allen wrench to remove the oil filler plug. Oil filler plug Oil-level sight glass TP02689 Drain plug Use the 8-mm Allen wrench to remove the oil drain plug. Tip the pump toward the drain plug aperture and allow the oil to drain completely into the container.
Page 182 4 Maintenance Procedures When the oil drain plug is tightened, the plug seals by means of an Tip: O-ring. Compression is controlled by the O-ring groove depth in the plug. Increased torque does not improve the plug seal; it only makes the plug difficult to remove later.
Page 183: Replacing The Roughing Pump's Oil Demister Element
Replacing the roughing pump’s oil demister element Replacing the roughing pump’s oil demister element Replace the roughing pump’s oil demister element annually. This procedure is not required for an Edwards oil-free roughing Note: pump. Required materials • Chemical-resistant, powder-free gloves •...
Page 184 4 Maintenance Procedures Carefully remove the exhaust flange and oil demister element from the roughing pump. Oil demister element TP02693 Use the 10-mm wrench to remove the nut that secures the oil demister element to the exhaust flange. Spring Securing nut TP02686 March 14, 2014, 715004387 Rev.
Page 185 Replacing the roughing pump’s oil demister element Holding the oil demister element slightly elevated to prevent the loss of the spring, remove the exhaust flange from the oil demister element. TP02692 Remove the spring from the oil demister element. To avoid spreading contamination, dispose of the Warning: oil demister element in accordance with local environmental regulations.
Page 186 4 Maintenance Procedures To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when replacing the oil demister element. The pump oil can be irritant, or contaminated with biohazardous or toxic analyte accumulated during normal operation.
Page 187 Replacing the roughing pump’s oil demister element To avoid damage, do not overtighten the nut that secures Notice: the oil demister element onto the exhaust flange; ensure that only approximately 1 mm of thread is exposed beyond the nut when it is tightened.
Page 188: Appi/Apci Source-Changing The Uv Lamp Bulb
4 Maintenance Procedures APPI/APCI source—changing the UV lamp bulb Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool ® • Small Phillips (cross-head) screwdriver • 20-cm (8-inch) length of 4-mm nylon tube To avoid personal contamination with biohazards or Warning: toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant,...
Page 189: Appi/Apci Source-Cleaning The Lamp Window
APPI/APCI source—cleaning the lamp window To avoid breaking the bulb, do not use a screwdriver to Notice: push the bulb forward in the lamp drive assembly. Insert the length of 4-mm nylon tube through the back of the lamp drive assembly, and push the bulb forward.
Page 190 4 Maintenance Procedures To clean the lamp window: Prepare the instrument for working on the source (see page 88). Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure. Use methanol or isopropyl alcohol, applied to the lint-free cloth, to carefully clean the lamp window.
Page 191: Appi/Apci Source-Replacing The Appi Lamp Drive Seals
APPI/APCI source—replacing the APPI lamp drive seals APPI/APCI source—replacing the APPI lamp drive seals To avoid leaking of biohazardous or toxic materials, Warning: ensure the integrity of the source exhaust system. The APPI lamp drive assembly O-rings listed below must be renewed at intervals not exceeding 1 year, exactly as described in this section.
Page 192 4 Maintenance Procedures To avoid personal contamination with biohazards Warning: or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. To avoid electric shock, ensure that the instrument Warning: is in Standby mode before commencing this procedure.
Page 193 APPI/APCI source—replacing the APPI lamp drive seals Use the Phillips (cross-head) screwdriver to remove the 2 source enclosure, release-handle screws, and remove the handle. Source enclosure cover screws Lamp-drive cover screws Release handle TP03204 Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the remaining 2 lamp-drive cover screws, which were hidden by the release handle.
Page 194 4 Maintenance Procedures Take care not to drop the screws inside the lower cover. Tip: Lamp mounting flange Mounting-flange screw TP03205 12. Slide the lamp assembly, shaft, and flange out of the APPI source enclosure. The cables remain attached to the shaft, which you fully withdraw Tip: and lay on the bench beside the source enclosure.
Page 195 APPI/APCI source—replacing the APPI lamp drive seals 13. Using the small Phillips screwdriver, remove the electrode screw and repeller electrode. 14. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the two insulator screws. 15. Remove the PEEK insulator from the end of the mounting shaft. 16.
Page 196 4 Maintenance Procedures 19. Use the O-ring removal kit to carefully remove the shaft mounting flange O-ring from the APPI source enclosure side. Lamp mounting flange O-ring To avoid spreading contamination, dispose of the Warning: O-rings in accordance with local environmental regulations. They can be contaminated with irritant oil, or with biohazardous or toxic materials.
Page 197: Fitting The New Appi Lamp Drive Assembly O-Rings
APPI/APCI source—replacing the APPI lamp drive seals Fitting the new APPI lamp drive assembly O-rings Required materials • Chemical-resistant, powder-free gloves • 3-mm Allen wrench • Small Phillips (cross-head) screwdriver • Small Pozidriv screwdriver • 1:1 methanol/water • Lint-free cloth To avoid personal contamination with biohazards Warning: or toxic materials, and to avoid spreading contamination to...
Page 198 4 Maintenance Procedures Fit the mounting shaft insertion tool to the mounting shaft. Mounting shaft insertion tool To prevent damage to the mounting shaft O-rings, fit the Notice: mounting shaft insertion tool to the mounting shaft before fitting the shaft to the lamp mounting flange. Slide the lamp mounting flange onto the shaft, taking care to align it correctly.
Page 199: Replacing The Instrument's Fuses
Replacing the instrument’s fuses 13. Use the small Phillips screwdriver to fit and tighten the repeller electrode retaining screw. 14. Insert the UV bulb into the lamp drive assembly and push it fully home. 15. Fully retract the lamp mounting shaft from the source enclosure. 16.
Page 200 4 Maintenance Procedures March 14, 2014, 715004387 Rev. B...
Page 201: A Safety Advisories
Warning symbols................202 Notices ....................206 Prohibition symbol ................206 Warnings that apply to all Waters instruments and devices ..207 Warnings that address the replacing of fuses ........ 212 Electrical and handling symbols ............. 214 March 14, 2014, 715004387 Rev. B...
Page 202: Warning Symbols
The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it.
Page 203: Specific Warnings
Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. To avoid injury from bursting, nonmetallic tubing, heed these Warning:...
Page 204 A Safety Advisories Mass spectrometer shock hazard The following warning applies to all Waters mass spectrometers. To avoid electric shock, do not remove the mass spectrometer’s Warning: protective panels. The components they cover are not user-serviceable. The following warning applies to certain mass spectrometers when they are in Operate mode.
Page 205 Warning symbols Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. To avoid infection with potentially infectious, human-sourced...
Page 206: Notices
A Safety Advisories Notices Notices appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample’s integrity. The exclamation point symbol and its associated statement alert you to such risk. To avoid damaging the instrument’s case, do not clean it with Notice: abrasives or solvents.
Page 207: Warnings That Apply To All Waters Instruments And Devices
Warnings that apply to all Waters instruments and devices Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Changes or modifications to this unit not expressly approved by the Attention: party responsible for compliance could void the user’s authority to operate the...
Page 208 A Safety Advisories Use caution when working with any polymer tubing under pressure: Warning: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. •...
Page 209 Warnings that apply to all Waters instruments and devices fare attenzione quando si utilizzano tubi in materiale polimerico Attenzione: sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante.
Page 210 A Safety Advisories 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. • 근처의 화기를 모두 끄십시오. • 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. •...
Page 211 Warnings that apply to all Waters instruments and devices The user shall be made aware that if the equipment is used in a Warning: manner not specified by the manufacturer, the protection provided by the equipment may be impaired. L’utilisateur doit être informé que si le matériel est utilisé d’une Attention: façon non spécifiée par le fabricant, la protection assurée par le matériel risque...
Page 212: Warnings That Address The Replacing Of Fuses
A Safety Advisories Warnings that address the replacing of fuses The following warnings pertain to instruments equipped with user-replaceable fuses. If the fuse types and ratings appear on the instrument: To protect against fire, replace fuses with those of the type Warning: and rating printed on panels adjacent to instrument fuse covers.
Page 213 Warnings that address the replacing of fuses If the fuse types and ratings do not appear on the instrument: To protect against fire, replace fuses with those of the type Warning: and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter.
Page 214: Electrical And Handling Symbols
A Safety Advisories Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse...
Page 215: Handling Symbols
Electrical and handling symbols Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Keep upright! Keep dry! Fragile! Use no hooks! March 14, 2014, 715004387 Rev. B...
Page 216 A Safety Advisories March 14, 2014, 715004387 Rev. B...
Page 217: B External Connections
To avoid damaging the mass spectrometer, observe the Notice: following precautions: • Contact Waters Technical Service before moving it. • If you must transport the instrument, or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
Page 218: External Wiring And Vacuum Connections
B External Connections External wiring and vacuum connections Rear panel connections appear in the figure below. Note that the connectors and controls not identified are for use by Waters engineers only. Rear panel: Event inputs and outputs Shielded Ethernet Roughing pump...
Page 219: Connecting The Oil-Filled Roughing Pump
10-mm reducer fitting (included in the installation kit) • 10-mm ID nylon tube (included in the installation kit) • PVC exhaust tubing (included in the Waters Rough Pump Connect Kit) • PVC hose clamps (included in the Waters Rough Pump Connect Kit) •...
Page 220 B External Connections Bear in mind these requirements when connecting the roughing pump: • The pump must be horizontal, or within one degree of horizontal. • The ambient temperature of the area in which you place the pump must range between 15 and 40 ºC. •...
Page 221 Connecting the oil-filled roughing pump Attach the NW25 tee to the inlet of the pump using the NW25 center ring, and then secure the connection with a clamp. 1-inch ID vacuum hose Clamps 10-mm nylon tube Flange NW25 tee 10-mm reducer fitting Pump inlet TP03414...
Page 222 B External Connections Route the open end of the exhaust tubing to a suitable exhaust vent. Check the oil level in the pump (see “Checking the roughing pump oil level” on page 106, and, if needed, “Adding oil to the roughing pump” on page 106).
Page 223: Connecting Electric Cables To The Oil-Filled Roughing Pump
Connecting the oil-filled roughing pump Connecting electric cables to the oil-filled roughing pump Roughing pump electrical connections: Detector rear panel Roughing pump d.c. connector Roughing pump main power connector To power source To make the electrical connections to the roughing pump: Connect the relay cable from the roughing pump d.c.
Page 224: Connecting The Edwards Oil-Free Roughing Pump
B External Connections Connecting the Edwards oil-free roughing pump To avoid electric shock, shut down the mass spectrometer, and Warning: disconnect all power cables from the oil-free roughing pump before performing maintenance procedures on the pump. Always carry out maintenance in accordance with the operator’s guide supplied with the roughing pump.
Page 225 • PVC hose clamps (included in the Waters Rotary Pump Kit) • 1-inch ID vacuum hose (included in the Waters Rotary Pump Kit) Bear in mind these requirements when connecting the roughing pump: • The pump must be horizontal, or within one degree of horizontal.
Page 226 B External Connections To connect the oil-free roughing pump: To avoid personal contamination with biohazards Warning: or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the pump and its connections. To avoid skeletal or muscle injury, at least two Warning: people must lift the pump.
Page 227: Connecting Electric Cables To The Edwards Oil-Free Roughing Pump
Connecting the Edwards oil-free roughing pump Use the sharp knife to cut the PVC exhaust tubing Requirement: squarely (that is, perpendicularly to its horizontal axis). Route the open end of the exhaust tubing to a suitable exhaust vent. Connect electric cables to the roughing pump (see page 227).
Page 228: Connecting To The Nitrogen Gas Supply
• Sharp knife • Wrench • 6-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) • Nitrogen regulator To connect the nitrogen gas supply: Use the sharp knife to cut a 3.8-cm to 5.0-cm (1.5-inch to 2-inch) length of 6-mm PTFE tubing.
Page 229 Connecting to the nitrogen gas supply Gas and exhaust connections: Source vacuum Turbo vacuum Collision cell gas inlet (Argon) Source vent Nitrogen inlet Attach the nitrogen regulator to the nitrogen supply. Install the 6-mm stud into the regulator outlet. Connect the free end of the long piece of 6-mm PTFE tubing to the 6-mm stud.
Page 230: Connecting To The Collision Cell Gas Supply
Connecting the nitrogen exhaust line Required materials • Chemical-resistant, powder-free gloves • Sharp knife • 10-mm and 12-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) ® • snoop (or equivalent) leak detector liquid March 14, 2014, 715004387 Rev. B...
Page 231 Connecting the nitrogen exhaust line To connect the nitrogen exhaust line: To avoid exposure to biohazards or toxic LC solvents Warning: carried in the nitrogen exhaust, ensure that the nitrogen exhaust is vented via the nitrogen exhaust trap bottle and laboratory exhaust system.
Page 232 B External Connections To prevent leaks in the source exhaust system, Warning: confirm the integrity of the system by performing the following leak test. Leaks can result in the release of biohazardous or toxic materials. To avoid damage to the instrument, use snoop (or its Notice: equivalent) leak detector liquid only for the purpose described in the following step.
Page 233: Connecting The Liquid Waste Line
Connecting the liquid waste line Connecting the liquid waste line Required material Chemical-resistant, powder-free gloves To connect the liquid waste line: To prevent personal contamination with biohazards or Warning: toxic materials, always wear chemical-resistant, powder-free gloves while performing this procedure. The waste line and connection can be contaminated.
Page 234 B External Connections To prevent leakage of biohazards or toxic Warning: materials, ensure that the, • drain line does not crimp or bend, impeding flow to the waste container. • waste container is emptied before the lower end of the drain tube is covered by waste solvent.
Page 235: Connecting The Workstation
Connecting the workstation Connecting the workstation To avoid injury from electric shock or fire, and to Warning: prevent damage to the workstation and ancillary equipment, do not place objects filled with liquid—such as solvent bottles—on these items, or expose them to dripping or splashing liquids.
Page 236: Connecting Ethernet Cables
B External Connections Connecting Ethernet cables Use shielded Ethernet cables with the instrument to ensure Requirement: compliance with FCC, and other, limits. To make Ethernet connections: Connect one end of one shielded Ethernet cable to the network switch, and then connect the other end to the Ethernet card on the ®...
Page 237: I/O Signal Connectors
I/O signal connectors I/O signal connectors To avoid electric shock, separate all electrical connections to Warning: the rear panel from hazardous voltages by double or reinforced insulation. Circuits of this type are classified as safety extra low voltage (SELV). Examples of circuits that are typically SELV include contact closure inputs and outputs for autosamplers, and UV, RI, and fluorescence detector signal outputs for LC/MS systems.
Page 238: Signal Connections
B External Connections Signal connections Instrument analog-out/event-in connections: Signal connections Description Analog (Out) Used for analog chart output functionality. The output voltage range is 0 to 1 V. The resolution of the voltage output is 12 bits. Stop Flow (Out) Used to stop the solvent flow if the nitrogen gas supply fails.
Page 239 I/O signal connectors To make the signal connections, attach the positive and negative leads of the signal cable to the connector. Connector Signal cable TP02585 Slide the clamp (with the bend facing down) into the protective shield. Insert the clamp and shield (with the bend facing down) into the connection cover, and loosely tighten with one self-tapping screw.
Page 240 B External Connections Insert the connector with the signal cable into the connection cover, and position the clamp over the cable leads. Tighten the clamp into place with the second self-tapping screw. Cable leads Clamp Place the second connection cover over the first cover, and snap it into place.
Page 241: Connecting To The Power Supply
United States and HAR-type (or better) in Europe. The main power cord must be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. Connect the female end of the power cord to the receptacle on the rear panel of the instrument.
Page 242 B External Connections March 14, 2014, 715004387 Rev. B...
Page 243: C Materials Of Construction And Compliant Solvents
Materials of Construction and Compliant Solvents To avoid injury caused by personal exposure to Warning: biohazards or toxic materials, you must confirm the integrity of the source exhaust system, and address any safety issues raised by the contents of this Appendix. Contents: Topic Page...
Page 244: Preventing Contamination
Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). Visit http://www.waters.com. Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents normally used with these items.
Page 245: Solvents Used To Prepare Mobile Phases
Solvents used to prepare mobile phases Items exposed to solvent: Item Material Trap bottle Polypropylene Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel, polybutylene terephthalate, and polyoxymethylene Solvents used to prepare mobile phases These solvents are the most common ingredients used to prepare mobile phases for reverse-phase LC/MS (API): •...
Page 246 C Materials of Construction and Compliant Solvents March 14, 2014, 715004387 Rev. B...
Page 247: D Plumbing The Intellistart Fluidics System
Plumbing the IntelliStart Fluidics System This appendix provides reference information for replacing the tubing in the IntelliStart™ Fluidics system. To avoid electric shock, do not use stainless steel Warning: tubing or stainless steel “finger tight” screws to connect the selector valve to the source probes; use the PEEK tubing and natural (beige) colored PEEK “finger tight”...
Page 248: Preventing Contamination
Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). You can find this document on http://www.waters.com; click Services and Support > Support. The selector valve The selector valve is located on the right-hand side of the instrument, behind the visor.
Page 249: Plumbing Schematic
Plumbing schematic Plumbing schematic Selector valve Waste column Xevo Pump Wash TQD probe Reservoir A Reservoir B Ensure that the end of the tubing is fully submerged in the Requirement: solvent in the wash reservoir. March 14, 2014, 715004387 Rev. B...
Page 250: Tubing And Connection Specifications
F196 F192 S, W, R, A and B F130 The tubing for the sample reservoirs (ports A and B) is not Important: user-serviceable. To replace the tubing, contact Waters to arrange an engineer visit. March 14, 2014, 715004387 Rev. B...

Waters Xevo TQD Overview And Maintenance Manual

Trademarks

Customer Comments

Safety Considerations

Operating this Instrument

EMC Considerations

EC Authorized Representative

1 Specifications and Operating Modes

Uses and Compatibility

Ionization Techniques and Source Probes

Intellistart Fluidics System

Ion Optics

MS Operating Modes

MS/MS Operating Modes

Sample Inlet

Leak Sensors

Vacuum System

Rear Panel Connections

2 Preparing for Operation

Starting the Mass Spectrometer

Preparing the Intellistart Fluidics System

Rebooting the Instrument

Leaving the Mass Spectrometer Ready for Operation

3 Changing the Mode of Operation

ESI Mode

Esci Mode

APCI Mode

Combined APPI/APCI Source

Nanoflow Source

4 Maintenance Procedures

Maintenance Schedule

Spare Parts

Troubleshooting with Connections INSIGHT

Safety and Handling

Preparing the Instrument for Operations on or Inside Its Source

Removing and Refitting the Source Enclosure

Installing and Removing the Corona Pin

Operating the Source Isolation Valve

Removing O-Rings and Seals

Cleaning the Instrument Case

Emptying the Exhaust Trap Bottle

Gas Ballasting the Roughing Pump

Checking the Roughing Pump Oil Level

Adding Oil to the Roughing Pump

Cleaning the Source Components

Cleaning the Sampling Cone Assembly

Cleaning the Extraction Cone

Cleaning the Ion Block Assembly

Cleaning the Ion Guide Assembly

Replacing the ESI Probe Tip and Gasket

Replacing the ESI Probe Sample Capillary

Cleaning the Ionsabre II Probe Tip

Replacing the Ionsabre II Probe Sample Capillary

Cleaning or Replacing the Corona Pin

Replacing the Ionsabre II Probe Heater

Replacing the Ion Block Source Heater

Replacing the Source Assembly Seals

Replacing the Air Filter

Replacing the Roughing Pump Oil

Replacing the Roughing Pump's Oil Demister Element

APPI/APCI Source-Changing the UV Lamp Bulb

APPI/APCI Source-Cleaning the Lamp Window

APPI/APCI Source-Replacing the APPI Lamp Drive Seals

Replacing the Instrument's Fuses

A Safety Advisories

Warning Symbols

Notices

Prohibition Symbol

Warnings that Apply to All Waters Instruments and Devices

Warnings that Address the Replacing of Fuses

Electrical and Handling Symbols

B External Connections

External Wiring and Vacuum Connections

Connecting the Oil-Filled Roughing Pump

Connecting the Edwards Oil-Free Roughing Pump

Connecting to the Nitrogen Gas Supply

Connecting to the Collision Cell Gas Supply

Connecting the Nitrogen Exhaust Line

Connecting the Liquid Waste Line

Connecting the Workstation