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Nikon DS-L4 Instructions Manual

Nikon DS-L4 Instructions Manual

Microscope camera control unit
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M668 E
16.9.Nx.1 (2/2)
DS-L4
Microscope Camera Control Unit
< Instructions >
Microscope Operation

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  • Page 1 M668 E 16.9.Nx.1 (2/2) DS-L4 Microscope Camera Control Unit < Instructions > Microscope Operation...

  • Page 3: Introduction

     No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.  The contents of this manual are subject to change without notice.

  • Page 4: Contents Of The Manual

    The instruction manual for the DS-L4 Microscope Camera Control Unit is provided in two volumes. Camera Operation (Describes the operation of the camera connected to the DS-L4.) Microscope Operation (Describes how to check the status and operate the microscope from the DS-L4 screen.) Symbols Used in This Manual The following symbols are used in this manual.

  • Page 5: Table Of Contents

    Symbols Used in This Manual ........................ ii Biological Microscope Motorized Units Operable from DS-L4 ..............1-1 Devices Operable From DS-L4 Connected to Ni-E ..............1-1 Devices Operable From DS-L4 Connected to Ni-U ..............1-3 Devices Operable From DS-L4 Connected to Ci-E ..............1-3 Basics of Microscope Operation with DS-L4 ............
  • Page 6 Contents (Details) Individual Operations ..................1-37 Operating Motorized Devices ....................1-37 Switching the Objective (Motorized Nosepiece) ....... 1-37 Switching the Optical Path (Motorized Quadrocular Tilting Tube) ..............1-40 Adjusting the Zoom Magnification (Motorized DSC Zooming Port) ................1-41 Switching the Filter Cube (Motorized Epi-fluorescence Cube Turret) ........
  • Page 7 ..1-92 Configuring the Connection of Motorized Shutter ........1-95 Configuring the Functions of Buttons ([BUTTON FUNCTION] Screen) ........1-96 Configuring the Screen Buttons for DS-L4 ..........1-96 Configuring Buttons on the Microscope .......... 1-102 Configuring the Function of the Ergo Controller Buttons .....
  • Page 8 Devices Whose Status Is Viewable on the DS-L4 Connected to ECLIPSE L200N/L200ND/L300N/L300ND ....................2-1 Devices Whose Status Is Viewable on the DS-L4 Connected to ECLIPSE MA200 ....2-2 Devices Whose Status Is Viewable on the DS-L4 Connected to ECLIPSE LV100DA-U ....2-2 Devices that can be Controlled and Monitored from DS-L4 Connected to ECLIPSE LV150A/LV150NA/LV-NCNT2 Nosepiece Controller 2/ LV-NCNT-N Motorized Nosepiece Controller ................2-3...

  • Page 9: Biological Microscope

    Part 1 Biological Microscope...
  • Page 11 Composition of Part 1 Part 1 describes how to operate the microscope or change the microscope’s settings from a DS-L4 Microscope Camera Control Unit connected to a biological microscope Ni-E, Ni-U, or Ci-E. Chapter 1 Motorized Units Operable from Introduction...

  • Page 13: Motorized Units Operable From Ds-L4

    “Camera Operation” instruction manual of DS-L4. By connecting the DS-L4 Microscope Camera Control Unit to a Ni/Ci series microscope, you can use the touch panel on the DS-L4 to control and monitor the microscope and accompanying motorized devices attached to the microscope.
  • Page 14 *1: With the focusing stage system, the stage is elevated, and with the focusing nosepiece system, the objective is elevated. *2: DS-L4 cannot be used to move the elevating section to an arbitrary position. *3: Motorized/intelligent epi-fluorescence cube turrets may be used in a single layer, or in two overlapping layers.

  • Page 15: Devices Operable From Ds-L4 Connected To Ni-U

    Chapter 1 Motorized Units Operable from DS-L4 Devices Operable From DS-L4 Connected to Ni-U Device Operation Available on DS-L4 Required Configuration ECLIPSE Ni-U Main Body None ON/OFF and brightness control of dia-illumination Information on camera connected Output of capture trigger signal...

  • Page 16: Basics Of Microscope Operation With Ds-L4

    The [MICROSCOPE CONTROL] screen appears. Shows or hides the menu tab. Note that if you are using microscope Ni-E or Ni-U, and a digital camera is not connected to DS-L4, the [MICROSCOPE CONTROL] screen appears after the initialization is completed.

  • Page 17: Screens For Ni-E

    The displayed content will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Motorized Units Operable from...

  • Page 18: Mic Easy Screen (Ni-E)

    Chapter 2 Basics of Microscope Operation with DS-L4 MIC EASY Screen (Ni-E) The [MIC EASY] screen provides buttons to control the microscope while observing images. The default button layout is shown below. Closes the [MIC EASY] screen A button for opening an operation sub screen (nosepiece, FL turret, etc.)
  • Page 19 Chapter 2 Basics of Microscope Operation with DS-L4 Microscope information (MIC INFO) on MIC EASY screen Press the [◄] or [►] button or swipe the microscope information area to move to a different page of information. (In the figure below, all displayable information is shown. If there is any motorized device unattached, that motorized device state is not displayed.)

  • Page 20: Info Window

    Chapter 2 Basics of Microscope Operation with DS-L4 INFO Window Optical path in motorized quadrocular tilting tube: BINO (Binocular) FRONT (Tube adapter) REAR (Rear port) DSC zooming port: Switches the displayed content. Zoom magnification First epi-fluorescence Closes the [INFO cube turret: MICROSCOPE] screen.

  • Page 21: Microscope Operation Buttons (Ni-E)

    DS-L4, refer to Chapter 1, “Motorized Units Operable from DS-L4”. Some buttons are not displayed by default, even if the corresponding device is operable from DS-L4. For details on showing/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.
  • Page 22 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-E) Function Description Chapter 5, “1.1 Switching Switches the objective. the Objective (Motorized Nosepiece)” [NOSEPIECE] [Objective (Address)] Directs the optical path in the motorized quadrocular tilting tube to the binocular section.
  • Page 23 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-E) Function Description Chapter 5, “1.10 Adjusting Adjusts the diameter of the the DIA Aperture Diaphragm aperture diaphragm in the (Motorized Universal motorized universal condenser. Condenser)” [A. STOP] Switches the ND for the HG precentered fiber illuminator.
  • Page 24 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-E) Function Description Opens/closes all HG Chapter 5, “1.6 precentered fiber illuminator’s Opening/Closing All built-in shutter, EPI motorized Shutters for shutter, epi-fluorescence cube Epi-illumination” [SHUTTER EPI ALL] turret’s built-in shutter.
  • Page 25 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-E) Function Description Chapter 5, “2.3 Loading a Loads a saved mode. Mode” [LOAD] [LOAD (MODE number)] Resets the Z-axis coordinate Chapter 5, “1.18.1 displayed on DS-L4 to zero Zero-resetting the Z-axis (0.000um).

  • Page 26: Screens For Ni-U

    The displayed content will differ depending on the configuration and settings of your microscope. The screen only shows devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen differs depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Motorized Units Operable from...

  • Page 27: Mic Easy Screen (Ni-U)

    Chapter 2 Basics of Microscope Operation with DS-L4 MIC EASY Screen (Ni-U) The [MIC EASY] screen provides buttons to control the microscope while observing images. The default button layout is shown below. Closes the [MIC EASY] screen A button for opening an operation sub screen (nosepiece, FL turret, etc.)

  • Page 28: Info Window

    Chapter 2 Basics of Microscope Operation with DS-L4 Microscope information (MIC INFO) on MIC EASY screen Press the [◄] or [►] button or swipe the microscope information area to move to a different page of information. (In the figure below, all displayable information is shown. If there is any motorized device unattached, that motorized device state is not displayed.)

  • Page 29: Microscope Operation Buttons (Ni-U)

    DS-L4, refer to Chapter 1, “Motorized Units Operable from DS-L4”. Some buttons are not displayed by default, even if the corresponding device is operable from DS-L4. For details on showing/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.
  • Page 30 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-U) Function Description Switches the ND for the HG precentered fiber illuminator. Chapter 5, “1.12 Operating [Intensilight (ND number)] the Motorized HG Precentered Fiber Opens/closes the HG Illuminator (Intensilight)” [INTSL] precentered fiber illuminator’s...
  • Page 31 Chapter 2 Basics of Microscope Operation with DS-L4 Operation Button (Ni-U) Function Description Outputs the capture trigger signal to the digital camera connected to the tube adapter. [CAPTURE FRONT] Outputs the capture trigger signal to the digital camera connected to the DSC zooming Chapter 5, “1.17...

  • Page 32: Screens For Ci-E

    Chapter 2 Basics of Microscope Operation with DS-L4 Screens for Ci-E The displayed content will differ depending on the configuration and settings of your microscope. When the motorized swing-out condenser is not attached, the status display and operation of the condenser are disabled.

  • Page 33: Info Window

    Chapter 2 Basics of Microscope Operation with DS-L4 Microscope information (MIC INFO) on MIC EASY screen Nosepiece: Objective series name (brevity code), magnification/numerical Status of the motorized swing-out aperture, immersion type condenser’s top lens: LENS IN (lens in optical path)/...

  • Page 34: Microscope Operation Buttons (Ci-E)

    Chapter 2 Basics of Microscope Operation with DS-L4 Microscope Operation Buttons (Ci-E) The buttons displayed on the [MIC EASY] screen have the following functions. When the motorized swing-out condenser is not attached, the operation button for the condenser is not displayed.

  • Page 35: Preparation For Microscopy

    Preparation for Microscopy Preparation for Microscopy This chapter describes various settings required for using DS-L4 to control your microscope. Configuring Optical Elements Information In this chapter, you configure the information on the optical elements attached to the microscope. The information of the optical elements you entered here is displayed on the DS-L4 screen.

  • Page 36: Configuring The Filter Cube Information

    Chapter 3 Preparation for Microscopy Configuring the Filter Cube Information In this section, you configure the information for filter cubes attached to the motorized or intelligent epi-fluorescence cube turret. When using Ni-E with motorized (or intelligent) epi-fluorescence cube turrets in two layers, be sure to configure the filter cube information for the second (upper) turret also.

  • Page 37: Configuring The Digital Camera Connection

    DSC connector on the microscope. This configuration is unnecessary if you are capturing with a camera head directly connected to this DS-L4 by pressing the [CAPTURE] button on the camera control screen.

  • Page 38: Microscopy With Ds-L4

    Microscopy with DS-L4 Microscopy with DS-L4 This chapter describes the microscopy operation procedures for DS-L4, using bright-field microscopy with Ni-E (Focusing Nosepiece System) microscope as an example. The following example assumes that the following devices are attached to the Ni-E microscope.
  • Page 39 Power switch Open the [MICROSCOPE CONTROL] screen. Press the [MENU] button on the DS-L4 screen, then press the [MIC CNTRL] button to switch to the [MICROSCOPE CONTROL] screen. Turn on the dia-illumination lamp and adjust the brightness. Press the [LAMP CTRL] button on the DS-L4 screen to control the dia-illumination lamp from DS-L4.
  • Page 40 ND filters. Set the optical path of the tube to 100% light distribution to the binocular section. Press the [BINO] button on the DS-L4 screen. Three buttons are provided for switching the optical path in the tube. The button for the currently selected optical path is shown with a check mark.
  • Page 41 Microscopy with DS-L4 Fully open the DIA field diaphragm and the DIA aperture diaphragm. Press the [F. STOP] button on the DS-L4 screen to open the sub screen. Move the slider to the right end. Press the [A. STOP] button on the DS-L4 screen to open the sub screen.
  • Page 42 Microscopy with DS-L4 Place a specimen on the stage, and move the stage to bring the target into view. Press the [XYZ] button on the DS-L4 screen to open the sub screen. Press the [Specimen Removal Position] button to move the stage.
  • Page 43 Chapter 4 Microscopy with DS-L4 Focus on the sample. Rotate the focus knob on the microscope main body, ergo controller, or joystick to operate the elevating section of the microscope main body. Z-axis coordinate Notes on controlling the focus knobs ...
  • Page 44 Coincide the fields of view for the right and left. Focus and center the condenser. Press the [F. STOP] button on the DS-L4 screen to stop Field Field down the DIA field diaphragm to the minimum, and look into diaphragm diaphragm the eyepiece.
  • Page 45 Microscopy with DS-L4 Motorized bright-field microscopy operation Bring an arbitrary objective into the optical path. Press the [NOSEPIECE] button on the DS-L4 screen to open the sub screen. Select the desired objective. When using an oil immersion type objective, apply immersion oil between the specimen and the objective.
  • Page 46 Chapter 4 Microscopy with DS-L4 Adjust the DIA aperture diaphragm. Press the [A. STOP] button on the DS-L4 screen to adjust the diameter of the aperture diaphragm. The aperture should be adjusted to about 70 to 80% of the numerical aperture of the objective.
  • Page 47 Chapter 4 Microscopy with DS-L4 Adjust the DIA field diaphragm. Press the [F. STOP] button on the DS-L4 screen to adjust the field diaphragm so that it almost circumscribes the field of view. Size of the field diaphragm Normally, the field diaphragm should be adjusted so that it almost circumscribes the field of view.
  • Page 48 Chapter 4 Microscopy with DS-L4 Capture images. Press the [REAR] button on the DS-L4 screen to direct the optical path of the quadrocular tilting tube to the rear port (motorized DSC zooming port), to which the digital camera is connected.

  • Page 49: Individual Operations

    The screen contents will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of your microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Motorized Units...
  • Page 50 Chapter 5 Individual Operations Operation with the [NOSEPIECE] Button Display Status of the [NOSEPIECE] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Displayed by default Displayed by default Ni-U Can be displayed by setting Displayed by default Ci-E Displayed (cannot be changed) (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.) [Procedure] Press the [NOSEPIECE] button.
  • Page 51 Chapter 5 Individual Operations Direct Operation with [Objective (Address)] Buttons Display Status of [Objective (Address)] Buttons [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Can be displayed by setting Can be displayed by setting Ni-U Displayed by default Can be displayed by setting Ci-E Cannot be displayed (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.)

  • Page 52: Switching The Optical Path (Motorized Quadrocular Tilting Tube)

    Chapter 5 Individual Operations Switching the Optical Path (Motorized Quadrocular Tilting Tube) Use the [BINO], [FRONT], or [REAR] button to redirect the optical path in the motorized quadrocular tilting tube to the binocular section, tube adapter, or rear port. Prerequisite for motorized operation A motorized quadrocular tilting tube must be attached to the microscope in order to perform the following operation.

  • Page 53: Adjusting The Zoom Magnification (Motorized Dsc Zooming Port)

    Chapter 5 Individual Operations Adjusting the Zoom Magnification (Motorized DSC Zooming Port) Use the [ZOOM] button to adjust the zoom magnification of the motorized DSC zooming port. Prerequisite for motorized operation A motorized DSC zooming port must be attached to the microscope in order to perform the following operation. Display Status of the [ZOOM] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen...

  • Page 54: Switching The Filter Cube (Motorized Epi-Fluorescence Cube Turret)

    (Address)] buttons. Prerequisite for motorized operation In order to switch the filter cube from DS-L4, a motorized epi-fluorescence cube turret must be attached to the microscope. When an intelligent epi-fluorescence cube turret is attached, the filter cube information is displayed, but the operation is disabled.
  • Page 55 Chapter 5 Individual Operations Direct Operation with [Filter Cube (Address)] Buttons Display Status of the [Filter Cube (Address)] Buttons (For the First Epi-fluorescence Cube Turret) [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Can be displayed by setting Can be displayed by setting Ni-U Can be displayed by setting Can be displayed by setting...

  • Page 56: Opening/Closing The Motorized Epi-Fluorescence Cube Turret's Built-In Shutter

    Use the [SHUTTER FL] button to open/close the motorized epi-fluorescence cube turret’s built-in shutter. Prerequisite for motorized operation In order to open/close the turret’s built-in shutter from DS-L4, a motorized epi-fluorescence cube turret must be attached to the microscope. When an intelligent epi-fluorescence cube turret is attached, the status of the built-in shutter is not displayed.
  • Page 57 Chapter 5 Individual Operations [Procedure] Press the [FL TURRET] or [SHUTTER] button. [MIC EASY] Screen (This operation is not required if the [SHUTTER FL] button is already displayed. Go to the next step.) Press the [SHUTTER FL] button. [MICROSCOPE CONTROL] Screen The shutter opens or closes each time the button is pressed.

  • Page 58: Opening/Closing All Shutters For Epi-Illumination

    Chapter 5 Individual Operations Opening/Closing All Shutters for Epi-illumination The following epi-illumination shutters are opened/closed at once.  Motorized HG precentered fiber illuminator’s built-in shutter  EPI motorized shutter  Motorized epi-fluorescence cube turret’s built-in shutter (in both turrets, if two are used in layers) The [SHUTTER EPI ALL] button is used for this operation.

  • Page 59: Switching The Excitation Filter (Motorized Excitation Filter Wheel)

    Chapter 5 Individual Operations Switching the Excitation Filter (Motorized Excitation Filter Wheel) To switch the excitation filter by rotating the motorized excitation filter wheel, use the [EX WHEEL] button or the [Excitation Filter (Address)] buttons. Prerequisite for motorized operation A motorized excitation filter wheel must be attached to the microscope in order to perform the following operation. Cautions on switching the excitation filter When switching the excitation filter, depending on the positioning of the optical parts, passing an empty address (that has no filter) may result in strong light entering the observation section and into your eyes.
  • Page 60 Chapter 5 Individual Operations Direct Operation with [Excitation Filter (Address)] Buttons Display Status of [Excitation Filter (Address)] Buttons [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Can be displayed by setting Can be displayed by setting (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.) [Procedure] Press an [Excitation Filter (Address)] button.

  • Page 61: Switching The Barrier Filter (Motorized Barrier Filter Wheel)

    Chapter 5 Individual Operations Switching the Barrier Filter (Motorized Barrier Filter Wheel) To switch the barrier filter by rotating the motorized barrier filter wheel, use the [BA WHEEL] button or the [Barrier Filter (Address)] buttons. Prerequisite for motorized operation A motorized barrier filter wheel must be attached to the microscope in order to perform the following operation. Operation with the [BA WHEEL] Button Display Status of the [BA WHEEL] Button [MICROSCOPE CONTROL] Screen...
  • Page 62 Chapter 5 Individual Operations Direct Operation with [Barrier Filter (Address)] Buttons Display Status of [Barrier Filter (Address)] Buttons [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Can be displayed by setting Can be displayed by setting (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.) [Procedure] Press a [Barrier Filter (Address)] button.

  • Page 63: Switching The Condenser Module (Motorized Universal Condenser)

    Chapter 5 Individual Operations Switching the Condenser Module (Motorized Universal Condenser) To switch the condenser module in the motorized universal condenser, press the [CONDEN.] button or a [Condenser Module (Address)] button. Prerequisite for motorized operation A motorized universal condenser must be attached to the microscope in order to perform the following operation. Operation with the [CONDEN.] Button Display Status of the [CONDEN.] Button [MICROSCOPE CONTROL] Screen...
  • Page 64 Chapter 5 Individual Operations Direct Operation with [Condenser Module (Address)] Buttons Display Status of [Condenser Module (Address)] Buttons [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Can be displayed by setting Can be displayed by setting (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.) [Procedure] Press the [Condenser Module (Address)] button.

  • Page 65: Adjusting The Dia Aperture Diaphragm (Motorized Universal Condenser)

    Chapter 5 Individual Operations Adjusting the DIA Aperture Diaphragm (Motorized Universal Condenser) 1.10 To adjust the aperture diaphragm in the motorized universal condenser, use the [A. STOP] button. Prerequisite for motorized operation A motorized universal condenser must be attached to the microscope in order to perform the following operation. Display Status of the [A.

  • Page 66: Swinging Out The Condenser Top Lens (Motorized Swing-Out Condenser)

    Chapter 5 Individual Operations Swinging Out the Condenser Top Lens (Motorized Swing-out Condenser) 1.11 To swing out the top lens of a motorized swing-out condenser, use the [CONDEN.] button. Prerequisite for motorized operation A motorized swing-out condenser must be attached to the microscope in order to perform the following operation. Display Status of the [CONDEN.] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen...

  • Page 67: Operating The Motorized Hg Precentered Fiber Illuminator (Intensilight)

    Chapter 5 Individual Operations Operating the Motorized HG Precentered Fiber Illuminator (Intensilight) 1.12 To open/close the shutter built into the motorized HG precentered fiber illuminator (Intensilight), use the [SHUTTER INTSL] button. To switch the ND built into the motorized HG precentered fiber illuminator, use the [INTSL] button or the [Intensilight (ND number)] buttons.
  • Page 68 Chapter 5 Individual Operations Switching the ND for the Motorized HG Precentered Fiber Illuminator 1.12.2 Operation with the [INTSL] Button Display Status of the [INTSL] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Displayed by default Displayed by default Ni-U Displayed by default Displayed by default (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.)

  • Page 69: Adjusting The Nd Filter For Dia-Illumination (Motorized Nd Filter Wheel)

    Chapter 5 Individual Operations Adjusting the ND Filter for Dia-illumination (Motorized ND Filter Wheel) 1.13 To adjust the transmittance of the ND filter in the motorized ND filter wheel, use the [ND WHEEL] button. Prerequisite for motorized operation A motorized ND filter wheel must be attached to the microscope in order to perform the following operation. Display Status of the [ND WHEEL] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen...

  • Page 70: Adjusting The Dia-Illumination Lamp/Led

    To control the dia-illumination lamp/LED from DS-L4, you must first transfer the control from the microscope to DS-L4. If DS-L4 does not have control, the button will be grayed out and disabled. When DS-L4 has control, the dia-illumination lamp/LED can also be controlled from the PC. Control of the dia-illumination lamp/LED is returned to the microscope main body when the microscope is turned off.
  • Page 71 ON/OFF. The lamp/LED turns ON/OFF each time the [LAMP ON/OFF]/[LED ON/OFF] button is pressed. (DS-L4 must have control of the lamp/LED. For details on the control, refer to “1.14.1 Transferring the Control of the Dia-illumination Lamp/LED”.) Adjusting the Brightness of the Dia-illumination Lamp/LED 1.14.3...

  • Page 72: Adjusting The Dia Field Diaphragm

    Chapter 5 Individual Operations Adjusting the DIA Field Diaphragm 1.15 To adjust the DIA field diaphragm in the microscope main body, use the [F. STOP] button. Display Status of [F. STOP] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen Ni-E Displayed by default Displayed by default (For details on displaying/hiding the buttons, refer to Chapter 6, “4.1.1 Selecting the Buttons to be Displayed”.) [Procedure]...

  • Page 73: Opening/Closing The Epi/Dia/Aux Motorized Shutter

    Chapter 5 Individual Operations Opening/Closing the EPI/DIA/AUX Motorized Shutter 1.16 To open or close the motorized shutter attached to the microscope, use the [SHUTTER EPI/DIA/AUX] button. Prerequisite for motorized operation A motorized shutter must be attached to the microscope in order to perform the following operation. Display Status of [SHUTTER EPI/DIA/AUX] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen...

  • Page 74: Outputting Capture Trigger Signals From The Microscope

    Use the [CAPTURE] button on the camera control screen. When you press these buttons, the camera head directly connected to this DS-L4 via a DS camera cable captures the image. You do not need to configure the connection in the [CONNECTION] screen.
  • Page 75 When using Ni-E, the following operations can be configured to be automatically performed when a capture trigger signal is output by pressing the [CAPTURE] button on the microscope main body or the [CAPTURE FRONT/LEFT/RIGHT/AUX] button on the DS-L4 screen. ...

  • Page 76: Operating The Elevating Section And The Motorized Xy Stage

    Zero-resetting the Z-axis Coordinate 1.18.1 The coordinate of the elevating section displayed on the DS-L4 screen can be reset to zero (0.000 m) as a current position. The value on the display panel of the microscope main body is also reset to zero.
  • Page 77 Chapter 5 Individual Operations Retracting the Elevating Section 1.18.2 [Procedure] Press the [XYZ] button. (This operation is not required if the [ESCAPE] button is already displayed on the [MIC EASY] screen. Go to the next step.) Press the [ESCAPE] button. [MICROSCOPE CONTROL] Screen The elevating section is moved to the position specified for the [ESCAPE DISTANCE] and enters the escape state.
  • Page 78 Chapter 5 Individual Operations Retracting the Elevating Section and Moving the Stage to the Specimen Removal 1.18.3 Position Prerequisite for motorized operation A motorized XY stage must be attached to the microscope in order to perform the following operation. [Procedure] Press the [XYZ] button.

  • Page 79: Using The Mode Function

    Chapter 5 Individual Operations Using the MODE Function The MODE function allows the microscopy state (position and status of motorized devices) to be stored in microscope’s memory and restored at a push of a button. A single microscopy state is stored as a single mode. Up to eight modes can be stored and named with up to 4 characters.
  • Page 80 Chapter 5 Individual Operations [Procedure] [MIC SETUP]→[MODE] Press the mode button to be used. Press the [MODIFY] button, then the [OK] button. To cancel adding or saving, press the [CANCEL] button. Press the [INPUT] button to enter a name for the mode, and then press the [ENTER] button.
  • Page 81 Chapter 5 Individual Operations Select the motorized devices for which the state is to be restored. The device is selected or unselected each time the button is pressed. To return to the previous screen, press the [BACK] button. Press the [OK] button. You will be taken back to the [MODE] screen.

  • Page 82: Saving/Updating A Mode (State Of Motorized Devices)

    Chapter 5 Individual Operations Saving/Updating a Mode (State of Motorized Devices) To store the current state of the microscope as a mode, or to overwrite the previously stored state of motorized devices with the current state, use the [SAVE] button. Register the target devices in advance Before storing the state of motorized devices as a mode, you must specify the devices for which the state is restored.

  • Page 83: Loading A Mode

    Chapter 5 Individual Operations Loading a Mode To restore the microscopy state stored as a mode, use the [LOAD] button or the [LOAD (MODE number)] buttons. (For details on saving a mode, refer to “2.2 Saving/Updating a Mode (State of Motorized Devices)”.) Operation with the [LOAD] Button Display Status of the [LOAD] Button [MICROSCOPE CONTROL] Screen...

  • Page 84: Deleting A Mode

    Chapter 5 Individual Operations Deleting a Mode An unused mode can be deleted. When you delete a mode, the name of that mode returns to the default [MD (1 to 8)] and no motorized device is subject to LOAD. Deleting a mode does not delete the stored state of the motorized devices Once a mode is deleted, the device states can no longer be restored for the target devices of that mode.

  • Page 85: Entering The Sleep State (Noise Reduction)

    Chapter 5 Individual Operations Entering the Sleep State (Noise Reduction) To reduce the generation of noise by shutting off the supply of power to the motorized devices, use the [SLEEP] button. For operations that are disabled in sleep state, refer to the Ni-E instruction manual “Operation”.) Display Status of the [SLEEP] Button [MICROSCOPE CONTROL] Screen [MIC EASY] Screen...

  • Page 86: Operating The Motorized Xy Stage With The Position Specified On The Monitor

    Operating the Motorized XY Stage with the Position Specified on the Monitor Swipe (or drag with a mouse) the live image on the DS-L4 monitor to move the motorized XY stage. To use the function, the function must be enabled on the [UTILITY] screen on the [MIC SETUP].

  • Page 87: Operating The Motorized Xy Stage

    - A still or replayed image is displayed or the screen is in dual-screen mode, - An error or confirmation message is displayed, - The DS-L4 is in standby state (blank screen). - A function is selected on the [TOOLBAR] (the button of the selected function is framed in blue), or - A setup menu for camera control operations is displayed.

  • Page 88: Settings

    Chapter 6 Settings Settings This chapter describes the procedure to change the various settings of the microscope and DS-L4 from the screen on DS-L4. These configurations are performed on [MIC SETUP]. [MAIN] screen of [MIC SETUP] (LIVE/VIEW menu →[SETTINGS] → [MIC SETUP])

  • Page 89: Bulk Saving Of Settings ([Main] Screen)

    Chapter 6 Settings Bulk Saving of Settings ([MAIN] Screen) After making changes on the [MIC SETUP], be sure to press the [SAVE] button on the [MIC SETUP] - [MAIN] to save the settings to the microscope’s memory. If the microscope is turned off without saving settings, previous values are restored. [Procedure] After changing the settings, press the [MAIN] button to go back to the [MIC SETUP] - [MAIN] screen.

  • Page 90: Configuring Optical Elements Information ([Components] Screen)

    In this section, you configure the information of the objectives attached to the motorized nosepiece or the intelligent nosepiece. The configured objective information is shown on a screen on the DS-L4 (for Ni-E, also on the display panel of the microscope). In addition, it is used for various interlocked operations. If you have replaced or added objectives, you need to reconfigure the settings.
  • Page 91 Chapter 6 Settings Select the name of the series of the objective attached to the microscope and press the [NEXT] button. When [▲] and [▼] buttons are shown under the list, you can press [▲] or [▼] button to go to other pages. To quit setting, press the [X] button.
  • Page 92 Chapter 6 Settings Arbitrarily Configuring the Data of Objective 2.1.2 When the objective attached to the microscope is not on the list, you can arbitrarily configure the objective’s data. The objective’s data arbitrarily configured is registered to the memory (memory address 1 through 10). [Procedure] [MIC SETUP] →...
  • Page 93 Chapter 6 Settings Press the [MODIFY] button and then [OK] button. To cancel adding or saving, press the [CANCEL] button. Removing the data registered to the memory To remove the data of the objective registered to the memory address, press the [DELETE] button. If the objective data registered for a nosepiece address is the same as that deleted here, settings in the nosepiece address will also be removed.
  • Page 94 Chapter 6 Settings Select the immersion type of the objective attached to the microscope and press the [NEXT] button. (10) Press the [INPUT] button, enter the product code of the attached objective, and then press the [ENTER] button.  Pressing the button removes a character at the end of the data you entered.

  • Page 95: Configuring The Condenser Module Information

    In this section, you configure the information on the condenser module attached to the motorized universal condenser. The configured condenser module information is shown in a screen on the DS-L4 and the display panel of the microscope. If you have replaced or added condenser modules, you need to reconfigure the settings.
  • Page 96 Chapter 6 Settings Arbitrarily Configuring the Name of Condenser Module 2.2.2 When the condenser module attached to the microscope is not on the list, you can configure any name. The name arbitrarily configured is registered to the memory (memory address 1 through 10). [Procedure] [MIC SETUP] →...
  • Page 97 Chapter 6 Settings Press the [MODIFY] button and then [OK] button. To cancel adding or saving, press the [CANCEL] button. Removing the module name registered to the memory To remove the module name registered to a memory address, press the [DELETE] button. If the module name to be removed has been registered to a turret address, settings in the turret address will also be removed.

  • Page 98: Configuring The Filter Cube Information

    In this section, you configure information for the filter cube attached to the motorized or intelligent epi-fluorescence cube turret. The configured filter cube information is shown in a screen on the DS-L4 and the display panel of the microscope (only in Ni-E). If you have replaced or added filter cubes, you need to reconfigure the settings.
  • Page 99 Chapter 6 Settings Arbitrarily Configuring the Name of Filter Cube 2.3.2 When the filter cube attached to the microscope is not on the list, you can configure any name. The name arbitrarily configured is registered to the memory (memory address 1 through 10). If you are using two epi-fluorescence cube turrets (motorized or intelligent) by layering them on top of each other, you use a single list of registered names for both layers.
  • Page 100 Chapter 6 Settings Press the [MODIFY] button and then [OK] button. To cancel adding or saving, press the [CANCEL] button. Removing the filter cube name registered to the memory To remove the filter cube name registered to a memory address, press the [DELETE] button. If the filter cube name to be removed has been registered to an turret address, settings in the turret address will also be removed.

  • Page 101: Configuring The Excitation Filter/Barrier Filter Information

    The configured filter information is shown in a screen on the DS-L4 and the display panel of the microscope. If you have replaced or added filters, you need to reconfigure the settings.
  • Page 102 Chapter 6 Settings Arbitrarily Configuring the Name of Excitation/Barrier Filter 2.4.2 When the excitation/barrier filter attached to the microscope is not on the list, you can configure any name. The name arbitrarily configured is registered to the memory (memory address 1 through 10). [Procedure] [MIC SETUP] →...
  • Page 103 Chapter 6 Settings Press the [MODIFY] button and then [OK] button. To cancel adding or saving, press the [CANCEL] button. Removing the filter name registered to the memory To remove the filter name registered to a memory address, press the [DELETE] button. If the filter name to be removed has been registered to an wheel address, settings in the wheel address will also be removed.

  • Page 104: Setting The Connections Of Motorized Units ([Connection] Screen)

    This configuration is also necessary when using the CAPTURE button on the microscope to send a trigger signal for capturing. This configuration is unnecessary if you are capturing with a camera head directly connected to this DS-L4 by pressing the [CAPTURE] button on the camera control screen.
  • Page 105 When a digital camera is mounted in a position other than the [AUX] above [Nikon] Send the trigger signal for Nikon digital camera Digital camera manufacturer [Andor] Send the trigger signal for Andor digital camera...
  • Page 106 Chapter 6 Settings [Procedure] [MIC SETUP] → [CONNECTION] Press the button for the DSC connector to be configured (left). A sub screen opens to select the position of the connected camera head. Select [NOT-CONNECTED] if no camera is connected, otherwise select the position. Press the button for the DSC connector to be configured (right).

  • Page 107: Configuring The Connection Of Motorized Shutter

    EPI SHUTTER/DIA SHUTTER connector. In this case, turn off the microscope and DS-L4, then reconnect the motorized shutter to the EPI SHUTTER/DIA SHUTTER connector of the microscope (the control box B for Ni-U). Or, check the usage of the motorized shutter connected to the EPI SHUTTER/DIA SHUTTER connector and change the usage with this configuration.

  • Page 108: Configuring The Functions Of Buttons ([Button Function] Screen)

    Settings Configuring the Functions of Buttons ([BUTTON FUNCTION] Screen) [BUTTON FUNCTION] screen allows you to configure functions and display of the buttons on DS-L4 and the microscope. Configuring the Screen Buttons for DS-L4 Up to fifteen buttons to control the microscope can be placed on the [MICROSCOPE CONTROL] screen. In addition, up to twelve buttons to control the microscope can be placed on the [MIC EASY] screen.
  • Page 109 Chapter 6 Settings Main Category Shown in [MICROSCOPE Shown in [MIC EASY] Screen of Direct CONTROL] Screen Button Icon Operation Ni-E Ni-U Ni-E Ni-U Buttons [EX WHEEL] [EX WHEEL] [Excitation Filter (Address)] [BA WHEEL] [BA WHEEL] [Barrier Filter (Address)] [CONDEN.] [CONDEN.] [Condenser Module (Address)]...
  • Page 110 Chapter 6 Settings Main Category Shown in [MICROSCOPE Shown in [MIC EASY] Screen of Direct CONTROL] Screen Button Icon Operation Ni-E Ni-U Ni-E Ni-U Buttons ([SHUTTER EPI/DIA/AUX], [SHUTTER EPI ALL]) [SHUTTER] Displayed by placing the [SHUTTER] [SHUTTER] button [SHUTTER EPI ALL] Displayed by placing the [SHUTTER] [FL TURRET] button...
  • Page 111 Chapter 6 Settings Main Category Shown in [MICROSCOPE Shown in [MIC EASY] Screen of Direct CONTROL] Screen Button Icon Operation Ni-E Ni-U Ni-E Ni-U Buttons Displayed on [XYZ] sub screen of [XYZ] button [ESCAPE] Displayed on [XYZ] sub screen of [XYZ] button [Specimen Removal Position]...
  • Page 112 Chapter 6 Settings Select the function you want to assign. The selected function is shown with a check mark. Pressing the [CLEAR] button assigns no function to the button and the button becomes blank. Layout of direct operation buttons When placing direct operation buttons (buttons which directly operate devices such as objectives or condenser modules without showing sub screen), select a main category button (with a...
  • Page 113 Chapter 6 Settings Showing/Hiding the [SLEEP] Button 4.1.2 In this section, you configure whether to display the [SLEEP] button to use the sleep function. In order to display the [SLEEP] button on the [MIC EASY] screen, you must first place the [SLEEP] button in the screen. See “4.1.1 Selecting the Buttons to be Displayed”...

  • Page 114: Configuring Buttons On The Microscope

    Chapter 6 Settings Configuring Buttons on the Microscope In this section, you configure functions of buttons on the microscope. Setting the Function of the Function Buttons 4.2.1 You can assign any functions among the following functions to six function buttons on the front of the Ni-E main body. Function Icon Function Switches the condenser module by rotating the motorized universal condenser turret...
  • Page 115 Chapter 6 Settings Function Icon Function Loads a mode. [LOAD (MODE number)] Default setting Button Number Function CONDEN. (-) CONDEN. (+) EX WHEEL (-) EX WHEEL (+) BA WHEEL (-) BA WHEEL (+) [Procedure] [MIC SETUP] → [BUTTON FUNC] Press the [FUNCTION BUTTON SET] button under “Ni-E”...
  • Page 116 Chapter 6 Settings Select the function you want to assign. The selected function is shown with a check mark. Pressing the [CLEAR] button assigns no function to the button and the button display becomes blank. Repeat steps (2) and (3) for all buttons of which you want to change the functions.
  • Page 117 Chapter 6 Settings Changing the Motorized Shutter Operated with the FL SHUTTER Button 4.2.2 You can change the motorized shutter to be operated with the FL SHUTTER button on the Ni-E main body. Setting Item Setting Value Shutter to Be Operated [FL SHUTTER [INTSL] Built-in shutter in the motorized HG precentered fiber...
  • Page 118 Chapter 6 Settings Changing the Digital Camera Operated with the Microscope’s CAPTURE Button 4.2.3 The capture method when the CAPTURE button of the microscope is pressed can be changed. Setting Item Setting Value Capture Method [CAPTURE BUTTON] [OFF] Disables the operation of the CAPTURE button. [USB] Capturing by sending a communication command from the (Default for Ni-E/Ni-U)
  • Page 119 Chapter 6 Settings Changing the Motorized Device to be Operated with CW/CCW Button 4.2.4 You can change the motorized device to be operated with the CW/CCW buttons on the Ni-E main body. Target of the Operation Setting Item Setting Value CW/CCW buttons on the left CW/CCW buttons on the right [CW/CCW BUTTON]...
  • Page 120 Chapter 6 Settings Enabling/Disabling the Button Operation 4.2.5 In order to avoid incorrect operation, buttons on the microscope can be enabled or disabled. Setting Item Setting Value Description [Ni-E LEFT]/ ON (default) Operations using the left side buttons are enabled [Ci-E LEFT] Operations using the left side buttons are disabled.

  • Page 121: Configuring The Function Of The Ergo Controller Buttons

    Chapter 6 Settings Configuring the Function of the Ergo Controller Buttons You can change the functions of the ergo controller buttons. Prerequisite for configuration In order to configure this setting, the ergo controller must be connected. Configurable buttons are shown below. Button 1 (top left) Button 2 (top right) Target of the CW/CCW buttons (two)
  • Page 122 Chapter 6 Settings Configuring the target of the CW/CCW button You can assign two (A-side and B-side) operations among the following operations to the CW/CCW buttons on the ergo controller. Function Icon Target Motorized barrier filter wheel (switches the barrier filters) [BA WHEEL] Motorized excitation filter wheel (switches the excitation filters) [EX WHEEL]...
  • Page 123 Chapter 6 Settings [Procedure] [MIC SETUP] → [BUTTON FUNC] Press the [FUNCTION SET] button under [ERGO CONTROLLER]. Current settings are displayed. Press the button for which you want to change the motorized device assignment. (Left: A, right: B) A sub screen to select a motorized device appears. Pressing the [ALL CLEAR] button clears settings for all buttons.
  • Page 124 Chapter 6 Settings Setting the Functions of Buttons (1 to 5) Other than the CW/CCW Buttons You can assign any functions among twenty functions to the five buttons other than ergo controller’s [CW]/[CCW] buttons. The meaning of each button’s LED depends on the configured function. Function Icon Function Switches the operation mode of the focus knobs between coarse, fine, and...
  • Page 125 Chapter 6 Settings Function Icon Function Opens/closes the EPI motorized shutter. LED on: shutter closed, LED off: shutter open [SHUTTER EPI] Opens/closes the DIA motorized shutter. LED on: shutter closed, LED off: shutter open [SHUTTER DIA] Opens/closes the built-in shutter in the motorized HG precentered fiber illuminator.
  • Page 126 Chapter 6 Settings [Procedure] [MIC SETUP] → [BUTTON FUNC] (1) Press the [FUNCTION SET] button under [ERGO CONTROLLER]. Current settings are displayed. Press the button of which you want to change the function assignment. A sub screen to select a motorized device appears. Pressing the [ALL CLEAR] button clears settings for all buttons.

  • Page 127: Enabling/Disabling The Buttons On The Ci-E Remote Control Pad

    Chapter 6 Settings Enabling/Disabling the Buttons on the Ci-E Remote Control Pad In order to avoid incorrect operation, buttons on the remote control pad can be enabled or disabled. Setting Item Setting Value Description [TOP] ON (default) Button enabled Button disabled [Procedure] [MIC SETUP] →...

  • Page 128: Configuring The Movement Of Motorized Devices ([Movement] Screen)

    Chapter 6 Settings Configuring the Movement of Motorized Devices ([MOVEMENT] Screen) In the [MOVEMENT] screen, you can configure the movement of motorized devices. Configuring Interlocked Operation You can configure to interlock operations of multiple motorized devices. Switching of objective, zoom magnification, or optical path, or triggering of capture from the microscope (by USB communication command/trigger signal), etc., can be configured to trigger specified operations of other motorized devices.
  • Page 129 (For a manual zooming port, it is adjusted assuming that the 1.0x zoom is used.) Once you turn off the DS-L4, the values in the previous state are cleared and the default value is restored. See “5.1.2 Changing the Initial Value of the [INTELLIGENT]”...
  • Page 130 Chapter 6 Settings [Procedure] [MIC SETUP] → [MOVEMENT] Press the [SET] button under [OBJECTIVE INTERLOCK]. Current settings are displayed. If any change is required, press the button for the intended objective. Select relevant settings of escape state, aperture diaphragm, ND wheel, and condense. To quit setting, press the [X] button.
  • Page 131 However, once you turn off the DS-L4 and then turn it on, the values of the adjusted state are cleared and the initial values are restored.
  • Page 132 Chapter 6 Settings Automatically Switching the Movement Speed of the Microscope’s Elevating 5.1.3 Section and Motorized Stage The movement speed of the microscope’s elevating section and motorized stage operated with the ergo controller (or joystick) can be switched automatically according to the magnification of the objective. Prerequisite for configuration In order to perform this configuration, motorized (or intelligent) nosepiece, and ergo controller (or joystick) must be attached.
  • Page 133 Chapter 6 Settings Configuring the Parfocal Correction Function (Auto Link Focus) 5.1.4 Parfocal correction is a function to automatically correct the difference between focal points of objectives when objective is switched by configuring focal point of each objective in advance. Note that parfocal correction is not performed when you switch objectives when the objective is retracted using the [ESCAPE] button.
  • Page 134 Chapter 6 Settings Press the button for address 1 to bring the objective in address 1 into the optical path. Use the focus knob on the microscope to focus on the specimen. Press the [SET] button to save the focal point of the address 1.
  • Page 135 Chapter 6 Settings Enabling or disabling the parfocal correction In this section, you enable or disable the parfocal correction function. If you want to use the parfocal correction function, you have to set the focal point in advance. If the focal point is not set after attaching the objective, see the previous section “Configuring the focal point”.
  • Page 136 Chapter 6 Settings Configuring the Interlocked Operation with Switching of Zoom Magnification 5.1.5 The DIA field diaphragm and motorized ND filter wheel can be adjusted automatically when the zoom magnification is switched. Prerequisite for configuration In order to perform this configuration, the motorized DSC zooming port, motorized (or intelligent) nosepiece, and motorized quadrocular tilting tube must be attached.
  • Page 137 Chapter 6 Settings Configuring the Interlocked Operation with Switching of Optical Path 5.1.6 The DIA field diaphragm can be adjusted automatically when the optical path is switched. Prerequisite for configuration In order to perform this configuration, the motorized (or intelligent) nosepiece and motorized quadrocular tilting tube must be attached.
  • Page 138 [MICROSCOPE CONTROL] or [MIC EASY] screen or the [CAPTURE] button on the microscope. Interlocked operation is not performed when you capture the image using the camera head directly connected to the DS-L4 by pressing the [CAPTURE] button on the camera control screen. Device to Be...
  • Page 139 Chapter 6 Settings [Procedure] [MIC SETUP] → [MOVEMENT] Press the [PATH], [FL TURRET], and [FL 2nd] button under [CAPTURE INTERLOCK] to change the settings. Pressing each button opens a sub screen showing options. Select the desired interlocked operation. After making changes to the settings, be sure to press the [SAVE] button on the [MAIN] screen to save the settings to the microscope’s memory.

  • Page 140: Setting The Retracting Amount Of The Elevating Section

     You press the ESCAPE button on the Ni-E main body  You press the [ESCAPE] button on the DS-L4  You press the [SAMPLE REMOVE POSITION] button on the DS-L4  Retraction is interlocked with switching objectives Setting Item...

  • Page 141: Disabling The Rotation Of The Motorized Nosepiece Depending On The Position Of The Elevating Section

    Chapter 6 Settings Disabling the Rotation of the Motorized Nosepiece Depending on the Position of the Elevating Section This is a function to prevent objectives from touching the specimen. You can disable the nosepiece rotation if the elevating section is above the specified position. The configured upper limit is applied to all nosepiece addresses.

  • Page 142: Disabling The Reverse Rotation Of The Nosepiece

    Chapter 6 Settings Disabling the Reverse Rotation of the Nosepiece Since the focal depth of the objective with low magnification is deep, such an objective may be positioned close to the specimen when used. When the objective is switched to the high magnification objective in this situation, the tip of the objective may contact with the specimen.
  • Page 143 Chapter 6 Settings [Procedure] [MIC SETUP] → [MOVEMENT] Switch the setting by pressing the [NOT-LIMITED] or (For Ni-E/Ni-U) [LIMITED] button under [NOSEPIECE REVERSE ROTATION]. (For Ci-E) After making changes to the settings, be sure to press the [SAVE] button on the [MAIN] screen to save the settings to the microscope’s memory.

  • Page 144: Configuring The Toggle Function (Alternating Between Two Objectives)

    Chapter 6 Settings Configuring the Toggle Function (Alternating between Two Objectives) Toggle function allows you to view the specimen while alternately switching between two objectives configured in advance. This operation is performed with the microscope’s or ergo controller’s nosepiece forward rotation/reverse rotation buttons. Two pairs of objectives can be configured: toggle pattern 1 and toggle pattern 2.
  • Page 145 Chapter 6 Settings Enabling/Disabling the Toggle Function 5.5.1 [Procedure] [MIC SETUP] → [MOVEMENT] Press the [SET] button under [NOSEPIECE TOGGLE] (For Ni-E/Ni-U) (this operation is not required for Ci-E. Go to the next step.) Press the [OFF], [PATTERN 1], or [PATTERN 2] button (For Ni-E/Ni-U) under [NOSEPIECE TOGGLE] to select the action when nosepiece rotation/reverse rotation button is...
  • Page 146 Chapter 6 Settings Configuring the Addresses Used by Toggle Function 5.5.2 [Procedure] [MIC SETUP] → [MOVEMENT] Press the [SET] button under [NOSEPIECE TOGGLE] (For Ni-E) (this operation is not required for Ci-E. Go to the next step.) For [PATTERN 1] or [PATTERN 2], press two buttons (For Ni-E) for addresses you want to use for toggle function.

  • Page 147: Configuring Top Lens Swing-Out Of The Motorized Swing-Out Condenser

    Chapter 6 Settings Configuring Top Lens Swing-out of the Motorized Swing-out Condenser If the motorized swing-out condenser is attached, you can configure the system so that the condenser top lens is automatically swung out when switching to the low-magnification objective. Prerequisite for configuration In order to perform this configuration, the motorized swing-out condenser must be attached.

  • Page 148: Configuring Other Functions ([Utility] Screen, [Maintenance] Screen)

    Chapter 6 Settings Screen) Configuring Other Functions ([UTILITY] Screen, [MAINTENANCE] Setting the Display of the Ni-E Front Display Panel There are nine status display patterns for the front display panel of the Ni-E main body. Patters displayed at the startup can be changed by this setting.

  • Page 149: Enabling/Disabling The Operation Of The Elevating Section

    Chapter 6 Settings Enabling/Disabling the Operation of the Elevating Section You can enable or disable the focus knob to operate the elevating section of the main body. The ESCAPE button is not affected by this setting. Setting Item Target of the Configuration Setting Value Description [Ni-E]...

  • Page 150: Turning On/Off The Buzzer

    Chapter 6 Settings Turning ON/OFF the Buzzer You can enable or disable the buzzer which sounds when you press the operation buttons. Setting Item Target of the Configuration Setting Value Description The buzzer when you press the ON (default) Buzzer ON [Ni-E] operation buttons of Ni-E main body Buzzer OFF...

  • Page 151: Setting The Software Limits

    Chapter 6 Settings Setting the Software Limits You can configure the software limit position for the elevating section and motorized XY stage of the Ni-E main body. Prerequisite for configuration In order to set the limit in XY direction, the motorized XY stage must be attached. Stop position during actual operation When controlling the elevating section with the focus knob, the actual stop position is up to 20 m beyond the set limit position.
  • Page 152 Chapter 6 Settings Configuring the Limit Position for the Elevating Section 6.4.1 You can define a limit position for movement of the elevating section by actually moving it to the desired position. [Procedure] [MIC SETUP] → [UTILITY] Use the focus knob to move the elevating section to a point that you want to set as a limit position.
  • Page 153 Chapter 6 Settings Configuring the Limit Position of the Motorized XY Stage 6.4.2 [MIC SETUP] → [UTILITY] Use the ergo controller or joystick to move the motorized stage to a point that you want to set as the limit of movement. Press the [SET CURRENT POSITION AS MAX] or [SET CURRENT POSITION AS MIN] button for X or Y-axis under [SOFTWARE LIMIT].

  • Page 154: Setting The Driving Speed Of The Epi-Fluorescence Cube Turret

    Chapter 6 Settings Setting the Driving Speed of the Epi-Fluorescence Cube Turret When you use a filter cube which includes the thick dichroic mirror, you must lower the movement speed of the epi-fluorescence cube turret. If you are using two layered motorized epi-fluorescence cube turrets, configure 1st and 2nd layers separately. Prerequisite for configuration In order to perform this configuration, the motorized epi-fluorescence cube turret must be attached.

  • Page 155: Restoring The Factory Default Settings

    Chapter 6 Settings Restoring the Factory Default Settings You can restore the factory default settings. Doing this operation restores all settings that can be configured in the [MIC SETUP] (including the information for attached optical elements, microscope settings saved as a mode, and initial value of interlock setting [INTELLIGENT]) to the factory default settings.

  • Page 156: Displaying The Program Version

    Chapter 6 Settings Displaying the Program Version You can check the program version of the microscope system and the ergo controller. [Procedure] [MIC SETUP] → [MAINTENANCE] Check the information shown in the [PROGRAM VERSION] (For Ni-E/Ni-U) section. The program version of the microscope system is displayed.

  • Page 157: Troubleshooting

    If you detect problems that are not listed below or the problem still persists after measures are taken, turn off the device and contact your nearest Nikon representative. The following table shows only problems when operating the microscope. Refer to the separately provided “Camera Operation” instruction manual for DS-L4 hardware related problems such as DS-L4 not turning on. Display Problem...

  • Page 158: Operation

    Settings have been made with Ni the DS-L4 button. In this case, the selection on the [BUTTON FUNC] screen Setup Tool. status is not displayed on DS-L4. (This is not an of [MIC SETUP]. error.) 1-146...
  • Page 159 Function (Switching between Two Objectives)”. ■ Operation of the dia-illumination lamp/LED Give control to DS-L4. Dia-illumination lamp/LED can not be turned on or off from DS-L4 does not have control. See Chapter 5, “1.14 Adjusting the DS-L4. Dia-illumination Lamp/LED”. 1-147...

  • Page 160: Saving Settings

    Chapter 7 Troubleshooting ■ Capture operation Turn off the microscope, DS-L4, and the camera, Camera trigger cable is not then reconnect the camera trigger cable of the properly connected. digital camera to the microscope. Capturing with a digital camera Check the connection between the digital camera cannot be started.

  • Page 161: Industrial Microscope

    Part 2 Industrial Microscope...
  • Page 163 Composition of Part 2 Part 2 describes the method to connect a DS-L4 DS Camera Control Unit to an industrial microscope and display the status of the microscope on DS-L4. Chapter 1 Devices Whose Status Is Viewable on DS-L4 Chapter 2 Viewing the Status of Microscope on...

  • Page 165: Devices Whose Status Is Viewable On Ds-L4

    Procedure for Connecting Each Device For details on the procedure for connecting the microscope main body, DS-L4, and individual parts, refer to the instruction manual of your microscope and also the “Camera Operation” instruction manual of DS-L4.

  • Page 166: Devices Whose Status Is Viewable On The Ds-L4 Connected To Eclipse Lv100Da-U

    Devices Whose Status Is Viewable on the DS-L4 Connected to ECLIPSE MA200 When the DS-L4 is connected to ECLIPSE MA200, the status of the following devices is viewable on the DS-L4. Configurations required before using accessories vary depending on the type of accessories you use. Refer to the following table to perform the required configuration.

  • Page 167: Devices That Can Be Controlled And Monitored From Ds-L4 Connected To Eclipse Lv150A/Lv150Na/Lv-Ncnt2 Nosepiece Controller 2/ Lv-Ncnt-N Motorized Nosepiece Controller

    Devices Whose Status Is Viewable on the DS-L4 Connected to LV-INAD Adapter for Intelligent Nosepiece When the DS-L4 is connected to the LV-INAD Adapter for Intelligent Nosepiece, the status of the following devices is viewable on the DS-L4. Refer to the following table to perform the required configuration.

  • Page 168: Devices Whose Status Is Viewable On The Ds-L4 Connected To Smz18/Smz25 Stereo Microscope

    Devices Whose Status Is Viewable on the DS-L4 Connected to SMZ1270i Stereo Microscope When the DS-L4 is connected to SMZ1270i, the status of the following devices is viewable on the DS-L4. Configurations required before using accessories vary depending on the type of accessories you use. Refer to the following table to perform the required configuration.

  • Page 169: Viewing The Status Of Microscope On Ds-L4

    Viewing the Status of Microscope on DS-L4 Viewing the Status of Microscope on DS-L4 This chapter describes the procedure to power on and off the DS-L4 and the configuration of screens that show the information on the microscope. Powering On/Off the Devices Powering ON the devices and displaying the MIC EASY screen Turn on the powers of the microscope main unit and motorized parts.

  • Page 170: Information Screen For Eclipse L200N/L200Nd/L300N/L300Nd

    The displayed content will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Devices Whose Status Is Viewable...

  • Page 171: Information Screen For Eclipse Ma200

    The displayed content will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Devices Whose Status Is Viewable...

  • Page 172: Information Screen For Eclipse Lv100Da-U

    The displayed content will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Devices Whose Status Is Viewable...

  • Page 173: Information Screen For Eclipse Lv150A/Lv150Na/Lv-Ncnt2 Nosepiece Controller 2/Lv-Ncnt-N Motorized Nosepiece Controller

    The displayed content will differ depending on the configuration and settings of your microscope. The screen will only show devices that are connected to the microscope and recognized by DS-L4. For this reason, the composition of the screen will differ depending on the configuration of the microscope. For information on microscopes and devices that can be recognized by DS-L4, refer to Chapter 1, “Devices Whose Status Is Viewable...

  • Page 174: Information Screen For Lv-Inad Adapter For Intelligent Nosepiece

    Therefore, the display appearance will depend on the configuration of the microscope. See Chapter 1 “Devices for Which Their Status Can be Detected by DS-L4” for the devices that can be detected by the DS-L4 from your microscope and accessories.

  • Page 175: Information Screen For Smz18/Smz25 Stereo Microscope

    Therefore, the display appearance will depend on the configuration of the microscope. See Chapter 1 “Devices for Which Their Status Can be Detected by DS-L4” for the devices that can be detected by the DS-L4 from your microscope zooming body and accessories.

  • Page 176: Information Screen For Smz1270I Stereo Microscope

    Therefore, the display appearance will depend on the configuration of the microscope. See Chapter 1 “Devices for Which Their Status Can be Detected by DS-L4” for the devices that can be detected by the DS-L4 from your microscope and accessories.

  • Page 177: Settings

    After making changes to the settings, be sure to press the [SAVE] button on the [MIC SETUP] - [MAIN] screen to save the settings to the microscope’s memory (DS-L4’s memory if LV150A or LV-NCNT2, or memory of nosepiece controller if LV-NCNT-N). If the microscope is turned off without saving settings, previous values are restored. For more information, see “1 Bulk Saving of Settings ([MAIN] Screen)”...

  • Page 178: Configuring Optical Elements Information ([Components] Screen)

    Registration of calibration settings is required to use DS-L4's scale/measurement function. To use the DS-L4 scale/measurement function, make sure to register the calibration settings after configuring objective information. If the [X] button of the [MIC SETUP] screen is pressed after configuring the information of the objective, you are prompted to register the calibration setting.
  • Page 179 After configuring, be sure to press the [SAVE] button on the [MAIN] screen to save the settings to the microscope’s memory (DS-L4’s memory if LV150A or LV-NCNT2, or memory of nosepiece controller if LV-NCNT-N). If the microscope is turned off without saving settings, previous values are restored. For more information on saving, see “1 Bulk Saving of Settings ([MAIN] Screen)”...
  • Page 180 Chapter 3 Settings Arbitrarily Configuring the Data of Objective 2.1.2 When the objective attached to the microscope is not on the list, you can arbitrarily configure the objective’s data. (Only for MA200, L200N/L200ND, L300N/L300ND, LV150A, LV-NCNT2, LV-NCNT-N, and LV100DA-U) [Procedure] [MIC SETUP] →...
  • Page 181 Chapter 3 Settings Select the magnification of the objective attached to the microscope and press the [NEXT] button. To return to the previous screen, press the [BACK] button. Press the [INPUT] button, enter the numerical aperture of the attached objective, and then press the [ENTER] button.
  • Page 182 After configuring, be sure to press the [SAVE] button on the [MAIN] screen to save the settings to the microscope’s memory (DS-L4’s memory if LV150A or LV-NCNT2, or memory of nosepiece controller if LV-NCNT-N). If the microscope is turned off without saving settings, previous values are restored. For more information on saving, see “1 Bulk Saving of Settings ([MAIN] Screen)”...

  • Page 183: Configuring The Filter Cube Information (Only For Az100M, Lv100Da-U, Smz18, And Smz25)

    Chapter 3 Settings Configuring the Filter Cube Information (Only for AZ100M, LV100DA-U, SMZ18, and SMZ25) In this section, you configure the information for the filter cube attached to the motorized or intelligent epi-fluorescence cube turret. If you have replaced or added filter cubes, you need to reconfigure the settings. Prerequisite for configuration A motorized or intelligent epi-fluorescence cube turret must be attached to the microscope in order to perform this configuration.
  • Page 184 Chapter 3 Settings Arbitrarily Configuring the Name of Filter Cube (Only for LV100DA-U, SMZ18, and 2.2.2 SMZ25) When the filter cube attached to the microscope is not on the list, you can configure any name. Configuration procedures are described separately for LV100DA-U and SMZ18/SMZ25. See the relevant section. For LV100DA-U [Procedure] [MIC SETUP] →...
  • Page 185 Chapter 3 Settings Press the [OK] button. Filter cube is set to the turret address. After making changes to the settings, be sure to press the [SAVE] button on the [MAIN] screen to save the settings to the microscope’s memory. If the microscope is turned off without saving settings, previous values are restored. For more information on saving, see “1 Bulk Saving of Settings ([MAIN] Screen)”...

  • Page 186: Configuring The Eyepiece Information (Only For Smz18 And Smz25)

    Chapter 3 Settings Configuring the Eyepiece Information (Only for SMZ18 and SMZ25) This section describes the setup procedure for the eyepiece attached to the binocular section. [Procedure] [MIC SETUP] → [COMPONENTS] Select the [EYEPIECE] tab. Press the eyepiece button to be configured. If the eyepiece is displayed as [-----], information for the eyepiece is not configured.

  • Page 187: Configuring Accessory Device Connection ([Connection] Screen) (Only For Smz1270I)

    Chapter 3 Settings Configuring Accessory Device Connection ([CONNECTION] Screen) (Only for SMZ1270i) In the [CONNECTION] screen, you can configure the connection between the microscope and the P-CI coaxial epi illuminator. [Procedure] [MIC SETUP] → [CONNECTION] Press the [CONNECTED]/[NOT-CONNECTED] button. The [CONNECTED]/[NOT-CONNECTED] indication toggles each time you press the button.

  • Page 188: Troubleshooting

    If you detect problems that are not listed below or the problem still persists after measures are taken, turn off the device and contact your nearest Nikon representative. The following table shows only problems when operating the microscope. Refer to the separately provided “Camera Operation” instruction manual for DS-L4 hardware related problems such as DS-L4 not turning on. Display Problem...

  • Page 189: Operation

    (Only with LV150A, LV150NA, Motorized nosepiece is not Turn off the microscope (or nosepiece controller) LV-NCNT2, and LV-NCNT-N) properly connected to the and DS-L4, then connect or attach the motorized Buttons on the nosepiece does microscope or nosepiece nosepiece correctly. not work.

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