Image Acquisition In Other Observation Modes; Xz Observation Mode - Olympus FLUOVIEW FV1000 User Manual

APPLIED OPERATIONS

2-2-2 Image Acquisition in Other Observation Modes

2-2-2-1 XZ Observation Mode

Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-4-1 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-4-2 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-4-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-4-4 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-4-5 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-4-6 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-4-7 in [OPERATION])
Set the observation range.
(Section 2-2-1-4-8 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
OPERATION INSTRUCTIONS
Page
2 - 3 4
/Image Acquisition
The description in this section will be focused on the image acquisition operations in the XZ
observation mode that are not used in the XY observation modes (which are the operations
enclosed in in the chart on the next page). For other operations, see section 2-2-1,
"Image Acquisition in XY Observation Mode". The details of each operation will be described
in the subsequent sections.
Set the range of the multiple sections
to be observed (the Z-direction
scanning range),
(Section 2-2-2-1-1 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-4-10 in [OPERATION])
If the image
If the image
does not
becomes
become
clean
clean
Re-adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-4-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-1-2 in [OPERATION])
Set the observation line.
(Section 2-2-2-1-3 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-1-4 in [OPERATION])
Acquire image.
(Section 2-2-2-1-5 in [OPERATION])
Save image.
(Section 2-3-1 in [OPERATION])