Olympus FLUOVIEW FV1000 User Manual page 35

Confocal laser scanning biological microscope
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NOTE
Clip for immersion
objective
NOTE
Getting Started FLUOVIEW/
When focus is aligned with use of focus handle of microscope
or U-FH, uncheck checkbox of [Locked] on [Z Stage] sub
panel inside [Acquire] panel. (See 2-2-1-4-7 of this manual.)
When [Locked] checkbox is checked, the handle cannot be
operated.
The specimen may float during oil-immersed observation. In
this case, prepare an optional clip for immersion objective and
attach it as shown on the left.
If you want to use a differential interference unit in transmitted light observation,
refer to the instruction manual of your microscope.
With transmitted light differential interference observation
using an immersion objective, set the microscope's field
diaphragm so that it circumscribes the field of view. Otherwise
the contrast may become degrade.
Outline of LSM Observation Procedures
OPERATION INSTRUCTIONS
Page
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