Olympus FLUOVIEW FV1000 User Manual page 39

Confocal laser scanning biological microscope
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4.
When only fluorescence observation is required, disengage the transmitted DIC slider
(3) by setting the switch to the pulled-out position. When transmitted light differential
interference observation or simultaneous fluorescence + transmitted light differential
interference observation is required, engage the transmitted DIC slider and the optimum
transmitted light DIC for the objective in the light path by operating the universal
condenser (5).
5.
(1) Cube display window
(2) Analyzer
IX2-MDICT
(3) Transmitted light DIC slider
(5) Universal condenser
Getting Started FLUOVIEW/
For transmitted light observation, disengage any filter (4) from the light path.
When you perform transmitted observation using laser with BX61WI, use the filter knob
to disengage the LBD from the light path and engage the FR (Frost) into the light path.
Disengaging the FR (Frost) from the light path may generate interference fringes on an
image.
(4) Filters
LBD
ND6
ND25
When using
BX61WI,
LBD
FFR
(This figure shows
the case of BX)
Outline of LSM Observation Procedures
(6) Hand switch
U-HSTR2(U-FH)
OPERATION INSTRUCTIONS
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