Olympus FLUOVIEW FV1000 User Manual page 268

APPLIED OPERATIONS
/ Protocol processor
One Point!
The protocol to perform the following operations can be assembled after setting the
observation conditions.
Specifying the region for bleaching in the laser irradiation mode using REX mask
files.
In Track 4, select a REX mask file in [Mask] of [REX]. Then in the laser irradiation mode
specified by the REX mask file selected, images can be acquired. (When using the system
with AOTF.) To select a REX mask file, display the REX mask file in the image window in
advance.
<The setting example>
For details, see section 2-2-11, "Image Acquisition in the Laser Excitation Mode".
Setting bleaching region by use of rectangular image acquisition (RECT).
Protocol
Repeat 30 times
Get 5
Acquire
Save
Wait 5
Set Zoom Get one Wait 5 Acquire
Set bleaching images
Set Zoom Set Zoom
image
power to x2 image image
sec.
（bleach） sec.
image
portion
power to x10 power to x2
Track 1 Track 2 Track 3
Reference image acquisition:
Acquire an image with the Zoom power 2 x in Track 1 in XYT observation.
Photo-bleaching:
Expose laser to photo-bleach with 5 images of acquisition using ZoomIn mode in Track 2 in
XYT observation.
Recovery observation:
Acquire 3 images in XYT observation every 5 seconds with the zoom power 2 x, the field of
view is the same with the reference image, and the images are stored in Track 3.
<Example>
OPERATION INSTRUCTIONS
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