Olympus FLUOVIEW FV1000 User Manual page 32

Getting Started FLUOVIEW/
•
Transmitted light observation procedure
Start the system
• Turn the system power ON.
(Section 1-2-1)
• Start the FLUOVIEW software.
(Section 1-2-1)
Let the transmitted light DIC
slider into the light path.
Select the light path for 100%
binocular tube and focus on the
specimen.
(Section 1-2-2)
Select the LSM light path.
(Section 1-2-3)
When the combination using the laser
combiner is used, set the optimum ND
filters for the laser.
OPERATION INSTRUCTIONS
Page
1 - 1 0
Outline of LSM Observation Procedures
(Section 1-2-6)
Change the ND filter with a higher
transmittance filter.
Set the observation condition.
• Set the objective magnification.
(Section 1-2-7-1)
• Set the zoom ratio to 1X.
(Section 1-2-7-2)
• Set the channels.
(Section 1-2-7-3)
• Set the highest scan speed.
(Section 1-2-7-4)
• Select the XY observation mode.
(Section 1-2-7-5)
• Perform repeated scanning.
(Section 1-2-7-6)
If no image is
displayed.
Image is
Adjust PMT Voltage.
displayed in
the [Live]
panel of the
software.
If an image is
displayed
• Set the multiple sections to be
observed.
(Section 1-2-7-7)
• Set the area to be observed.
(Section 1-2-7-8)
• Set a lower scan speed.
(Section 1-2-7-9)
• Stop repeated scanning.
(Section 1-2-7-10)
Acquire an image.
(Section 1-2-8)
Save the image.
(Section 1-2-9)
Exit from the FLUOVIEW software.
(Section 1-2-10)
Turn the system power OFF.
(Section 1-2-10)
(Section 1-2-6)
(Section 2-2-1-4-9)