Olympus FLUOVIEW FV300 Manuals

Manuals and User Guides for Olympus FLUOVIEW FV300. We have 2 Olympus FLUOVIEW FV300 manuals available for free PDF download: User Manual, Overview

Olympus FLUOVIEW FV300 User Manual (564 pages)

CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPE

Brand: Olympus | Category: Microscope | Size: 17 MB

1 System Overview 1-1

11
- Table of Contents
  11
- System Overview
  13
1 Getting Started Fluoview 1-1

29
- Getting Started FLUOVIEW
  
  39
- Applied Operations
  
  115
  - General Operation Procedure
    115
    
    Image Acquisition Procedure (Section (A))
    117
    
    Image Acquisition Procedure in an Observation Mode (Section (B))
    118
    
    Examples of Operation Procedures
    119
  - Image Acquisition
    121
    
    Image Acquisition in XY Observation Mode
    122
    
    Configuring the Microscope
    123
    
    Configuring the Scan Unit
    127
    
    Setting the Observation Condition
    128
    
    Acquiring Image
    136
    
    Acquiring Image in Accumulation Mode
    136
    
    Saving the Acquired Image in File
    140
    
    Image Acquisition in Other Observation Modes
    141
    
    XZ Observation Mode
    141
    
    XT Observation Mode
    147
    
    XZT Observation Mode
    149
    
    XYZ Observation Mode
    155
    
    XYT Observation Mode
    160
    
    XYZT Observation Mode
    163
    
    Differences in Image Acquisition Method between Fluorescent and Transmitted Images
    170
    
    Monochrome Image
    170
    
    Dual-Fluorochrome Image
    174
    
    Transmitted Image
    177
    
    Image Acquisition by Rotating It (Rotation Scan)
    181
    
    Image Acquisition of Only the Rectangular Position (Clip Scan)
    182
    
    High-Speed Image Acquisition
    187
    
    Image Acquisition to Prevent Crosstalk between Fluorescence
    188
    
    Scan)
    188
    
    Virtual Channel Function
    191
    
    Image Acquisition of a Line at Desired Angle
    196
    
    Display the Change of Image Intensity (Point Scan)
    199
    
    Image Acquisition on Desired Line (XZ, XT or XZT Observation)
    203
    
    Image Acquisition in the Laser Excitation Mode
    206
    
    Making REX Mask File
    207
    
    Example of FRAP Experiment
    212
  - Saving, Opening and Shredding Images
    225
    
    Saving Images
    228
    
    Saving Images as a Series
    228
    
    Saving a Display
    230
    
    Saving Specified Area of Image
    233
    
    Saving Animation Images
    236
    
    File Types Available for Save
    238
    
    Opening Previously Saved Images
    246
    
    Shredding Images
    247
    
    Saving Comment Together with Image
    249
    
    Checking the Image Information/Acquisition Parameters
    251
    
    Saving the Image Information/Observation Condition
    255
    
    Saving the Region File
    258
    
    Saving/Reading the Region File
    258
    
    Reading the Region File
    260
  - Protocol Processor
    263
    
    Starting the Protocol Processor
    264
    
    Editing the Protocol
    265
    
    Description of Setting Items
    266
    
    Command List
    273
    
    Protocol Repetition Processing
    278
    
    Input Supporting Function
    279
    
    Protocol Procedure Supporting Function
    281
    
    Saving the Protocol
    282
    
    Executing the Protocol
    284
    
    Loading a Protocol
    286
    
    Loading a Protocol of Previous Format
    287
    
    COM Communication Function
    289
    
    Example of Assembling a Protocol
    290
    
    Example of Setting Procedure of FRAP Observation
    290
    
    Example of Setting Procedure of XYT Observation for Hours
    299
    
    Processing)
    299
    
    Pop-Up Menu
    307
    
    Restrictions of [PAPP] Setting
    308
    
    Restrictions with [Mode] and I/O Card
    308
    
    Restrictions with [Mode] and [Sub Mode]
    311
  - Changing the Image Display Method
    313
    
    Displaying an Image in Simulated Colors
    313
    
    Editing the LUT (Look up Table)
    315
    
    LUT Graph Editing According to Colors
    315
    
    LUT Graph Editing by Gamma Correction
    317
    
    Switching the Displayed Channels (Ch1, Ch2, Ch3)
    318
    
    Displaying Images of Multiple Channels Simultaneously
    319
    
    Displaying Images Separately Per Channel
    319
    
    Over and under Views. Single View)
    319
    
    Views)
    319
    
    Displaying Merged Image of Multiple Channels (Single Views)
    321
    
    Changing the Number of Divided Images
    322
    
    Increasing the Number of Divided Images
    322
    
    Decreasing the Number of Divided Images
    324
    
    Switching the Display Method of Multiple Images
    326
    
    Displaying Multiple Image Slices Together
    327
    
    Displaying Multiple Images Per Channel
    328
    
    Displaying Images of Two Channels Together
    329
    
    Displaying Time-Lapse Images
    330
    
    Displaying Multiple Cross-Section Images
    331
    
    Displaying same Images in Different Display Methods
    331
    
    Displaying Different Images Together
    333
    
    Re-Arranging Images Using the same Display Method
    333
    
    Displaying an Image in Full Screen
    336
    
    Magnifying/Reducing an Image
    338
    
    Filtering
    339
    
    Image Processing
    339
    
    Contour Enhancement
    340
    
    Noise Reduction
    343
    
    Image Sharpening
    344
    
    Correcting DIC Level Irregularities
    345
    
    Contrast Conversion
    347
    
    Image Addition
    350
    
    Mathematical Operations between Images
    350
    
    Image Division
    353
    
    Image Multiplication
    353
    
    Image Subtract
    353
    
    NOT Image
    354
    
    Image and Image
    356
    
    Image or Image
    358
    
    Image XOR Image
    358
    
    Brightness Overlap Level between 2 Channels (Colocalization)
    359
    
    Annotation Mode
    360
    
    Colocalization for Series Image Data Set
    367
    
    Appending Image (Append)
    371
    
    Appending Two Images
    371
    
    Appending Image from Several Image Data Set
    376
    
    Extract Image (Crop)
    381
    
    Extract Image Data from an Image Data Set
    381
  - Image Analysis
    387
    
    Checking the Intensity of a Specific Part
    388
    
    Intensity Values on a Line (Line Profile)
    388
    
    Intensity Values on a Planar Region (Bird's Eye View)
    391
    
    Checking the Intensity Distribution of a Specific Part
    395
    
    Intensity Distribution on a Line (Histogram)
    395
    
    Intensity Distribution on a Planar Region (Histogram)
    396
    
    Area Measurement
    398
    
    Length Measurement
    398
    
    Measuring a Part
    398
    
    Measuring the Change in Mean Value of Intensity
    399
    
    Measuring the Change in Integrated Intensity
    405
  - Building an Image from a Different Viewpoint
    407
    
    Building Extended Focus Image from XYZ Image
    407
    
    Display Switching to Built Image
    407
    
    Turning Built Image into Single Image
    410
    
    Building Line Images to be Viewed in Z Direction
    412
    
    Turning Built Image into Time Series Image
    412
  - Viewing 3D Image
    414
    
    Successive Display of Images
    416
    
    Changing the Successive Display Speed
    417
    
    Animation
    418
    
    Changing the Successive Image Display Position
    418
    
    Building Stereo 3D Images
    421
    
    Building a 3D Image to be Viewed through Color (Red/Green) Eyeglasses2-293
    423
  - Viewing Images Following the Progress of Time
    425
    
    Displaying Images Successively
    425
    
    Displaying Images Together
    425
  - Transferring Data to Another Application
    427
    
    Transferring Analysis Data to Another Application
    427
    
    Transferring the Plot Image of Analysis Data to Another Application
    430
    
    Transferring Image Data to Another Application (Microvoxel, Paint, Etc.)2-301
    431
  - Entering Comment in Image
    432
    
    Writing Characters in Image
    432
    
    Displaying the Image Intensity
    434
    
    Displaying the X-Coordinate/Y-Coordinate of the Image
    435
    
    Drawing a Figure in Image
    436
    
    Drawing a Scale in Image
    437
    
    Drawing an Arrow in Image
    439
    
    Drawing Color Bars in Image
    440
    
    Deleting Comment
    441
    
    Moving Comment
    441
    
    Changing the Comment Size
    442
    
    Changing the Comment Color
    443
    
    Changing the Comment Font
    444
    
    Image Output at Printer
    445
    
    Merger/Extraction of Image Channels
    447
    
    Setting the Range of Multiple Image Slices
    447
    
    Merging Image Channels
    448
    
    Extracting Channels from Image
    453
    
    Changing the Chart Display Method
    457
    
    Chart] Panel
    457
    
    Series] Panel
    465
    
    Pop-Up Menus
    468
- Appendix A List of Tools
  
  473
- Appendix A-1 List of Tools
  
  473
- Appendix A-1-1 Toolbar
  
  473
- Appendix A-1-2 Tools at the Top of Display Panel
  
  474
- Appendix B List of Hot Keys
  
  475
- Appendix C Glossary
  
  479
- Appendix D Formatting of Magnetic Optical Disk
  
  483
- Appendix E Converting Analysis Data into a Chart
  
  487
  - Using EXCEL
    487
- Appendix G-1 User Registration
  
  493
- Appendix F User Registration
  
  495
- Appendix G USER REGISTRATION of FV300
  
  495
- Appendix G-2 Logging into the FV300
  
  497
- Appendix G-3 Deleting a User
  
  498
- Appendix H Change of Default Folder for [File I/O] Panel
  
  501
- Appendix I List of Functions in the [Active Overlays] Dialog Box
  
  507
- Appendix I-1 Coordinate Position Data
  
  507
- Appendix I-1-1 X-Coordinate
  
  507
- Appendix I-1-2 Y-Coordinate
  
  508
- Appendix I-1-3 Other
  
  509
  - Position
    509
  - Animation
    509
- Appendix I-2 Intensity Data
  
  510
- Appendix I-3 Other
  
  511
- Appendix I-3-1 Channel Number
  
  511
- Appendix I-3-2 Objective Power
  
  511
- Appendix I-3-3 Date of Image Capturing
  
  511
- Appendix I-3-4 Time of Image Capturing
  
  511
- Appendix I-3-5 Image File Name
  
  511
- Appendix J Hand Switch and Microscope Frame Function Allocation
  
  513
- Appendix J-1 Hand Switch Functions
  
  513
- Appendix J-2 Microscope Frame Functions
  
  514
- Appendix J-2-1 BX
  
  514
- Appendix J-2-2 IX
  
  515
- Appendix J-2-3 Focus Adjustment Knob
  
  516
  - Software Setup
    521
    
    Re-Setup or Updating of the Software
    521
    
    New Setup of the Software
    523
    
    Overall Setting of FLUOVIEW
    526
    
    Setting the System Configuration
    526
    
    Setting the [Microscope Control Panel]
    534
    
    Adding the Dyeing Method
    541
    
    Associating a Detection Channel and Filter to the Dyeing Method
    544
  - Maintenance of Major System Units
    549
    
    Replacing the Barrier Filters Slider
    549
    
    Scan Unit
    549
    
    Laser Scanning Microscope
    550
1 Troubleshooting Guide 1-1

555

Olympus FLUOVIEW FV300 Overview (16 pages)

CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES

Brand: Olympus | Category: Microscope | Size: 2 MB

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Digital Camera Voice Recorder Microscope Camera Accessories Camera Lens

More Olympus Manuals

Olympus FLUOVIEW FV300 Manuals

Olympus FLUOVIEW FV300 User Manual (564 pages)

Table of Contents

1 System Overview 1-1

1 Getting Started Fluoview 1-1

Getting Started FLUOVIEW

Applied Operations

Appendix A List of Tools

Appendix A-1 List of Tools

Appendix A-1-1 Toolbar

Appendix A-1-2 Tools at the Top of Display Panel

Appendix B List of Hot Keys

Appendix C Glossary

Appendix D Formatting of Magnetic Optical Disk

Appendix E Converting Analysis Data into a Chart

Appendix G-1 User Registration

Appendix F User Registration

Appendix G USER REGISTRATION of FV300

Appendix G-2 Logging into the FV300

Appendix G-3 Deleting a User

Appendix H Change of Default Folder for [File I/O] Panel

Appendix I List of Functions in the [Active Overlays] Dialog Box

Appendix I-1 Coordinate Position Data

Appendix I-1-1 X-Coordinate

Appendix I-1-2 Y-Coordinate

Appendix I-1-3 Other

Appendix I-2 Intensity Data

Appendix I-3 Other

Appendix I-3-1 Channel Number

Appendix I-3-2 Objective Power

Appendix I-3-3 Date of Image Capturing

Appendix I-3-4 Time of Image Capturing

Appendix I-3-5 Image File Name

Appendix J Hand Switch and Microscope Frame Function Allocation

Appendix J-1 Hand Switch Functions

Appendix J-2 Microscope Frame Functions

Appendix J-2-1 BX

Appendix J-2-2 IX

Appendix J-2-3 Focus Adjustment Knob

1 Troubleshooting Guide 1-1

8 Circular Flare Is Observed at the Center of Image

9 Image Is Blurred or out of Focus

Olympus FLUOVIEW FV300 Overview (16 pages)

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