Olympus FLUOVIEW FV1000 User Manual page 133

Confocal laser scanning biological microscope
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2 Setting the numbers of steps and acquired image slices
[Step Size] text box
[Slices] text box
3 Setting the observation mode
4 Acquiring image
<Resume> button
Restarts image acquisition at
the frame next to the frame
where
suspended.
1. Set the number of steps using the <
TIP
The number of steps shown in the [Step Size] text box has been calculated by
the system so that the depth scale of the acquired image is identical to the
horizontal scale.
TIP
The number of acquired images shown in the [Slices] text box can also be input
from the keyboard.
After setting [Start Z] (Z-direction scan start position) and [Stop Z] (Z-direction
scanning stop position), input the desired number of images in the [Slices] text
box. This automatically sets [Step Size] (number of steps).
1.
In the [Mode] group box in the [Acquire] panel, select the [Surface] option button.
2.
In the [Acquire] panel, select the XYZ observation mode option button.
1.
Click the <XYZ> button in the [Acquire] panel.
The acquired image will be displayed in the [Live] panel.
While acquiring an image in the XYZ observation mode, clicking the <STOP SCAN> button
changes the buttons at the upper part of the [Acquire] panel as shown below. The
<Resume> button restarts image acquisition at the frame next to the frame where the
acquisition is suspended.
the
acquisition
is
APPLIED OPERATIONS
> or <
> button in the [Step Size] text box.
<Series Done> button
Determines
images. Once this button is
clicked, it is not possible to
append an image.
OPERATION INSTRUCTIONS
Page
/Image Acquisition
the
acquired
2 - 4 9

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