Olympus FLUOVIEW FV1000 User Manual page 188

APPLIED OPERATIONS
3 Making REX Mask File
OPERATION INSTRUCTIONS
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/Image Acquisition
3. Select the <Once> button in the [Acquire] panel and acquire an image.
If necessary, set the range for image acquisition with clip scan, for example, after
acquiring an image.
See section 2-2-12-1, "Making REX Mask File" for the procedure to make the REX mask file.
If the setup of the region of laser excitation remains after making the REX mask file, the
intensity change of the region can be observed in the TIME COURSE software (optional).
See the "FV-TIEMPO TIME COURSE software User's manual" for details of the TIME
COURSE software.