Olympus FLUOVIEW FV1000 User Manual page 266
Confocal laser scanning biological microscope
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Also See for FLUOVIEW FV1000:
- User manual (186 pages) ,
- Overview (28 pages) ,
- Short instructions (7 pages)
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APPLIED OPERATIONS
Setting Track 1
Setting Track2
<Skip> button
OPERATION INSTRUCTIONS
Page
2 - 1 8 2
/ Protocol processor
Set each Track as sown below;
In XYT observation, set the zoom ratio to 2X and acquire an image before bleaching.
1.
Select [New mode]-"XYT" in [Mode] of [Track].
2.
Select "Normal" in [Start by] of [Track].
3.
Enter "1" in [Condition] of [Track].
4.
Enter "0" to [Frame Interval].
5.
Enter "2" to [Zoom].
6.
Select "Middle" in [Scan Speed].
7.
Enter the laser intensity value for image acquisition to [Laser Intensity]
8.
Enter the PMT value of each channel to [PMT Voltage].
9.
Enter a comment in [Comment] if necessary.
In XYT observation, set the zoom ratio to 10X and acquire three images. (Irradiate strong
laser onto the region to bleach.)
1.
Select "Series mode" in [Mode] of [Track].
2.
Select "Normal" in [Start by] of [Track].
3.
Enter "3" to [Condition] of [Track].
4.
Enter "0" to [Frame Interval].
5.
Enter "10" to [Zoom].
6.
Select "Slow" in [Scan Speed] for fast photo-bleaching.
7.
Enter the laser intensity value for image acquisition to [Laser Intensity]
8.
Enter the PMT value of each channel to [PMT Voltage].
9.
Enter a comment in [Comment] if necessary
TIP
To properly irradiate the laser for bleaching, enter a larger value in [Condition]
and fast forward the tracks using the <Skip> button in accordance with the
grade of bleaching.
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