Olympus FLUOVIEW FV1000 User Manual page 150

Confocal laser scanning biological microscope
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APPLIED OPERATIONS
Ch1 check box
OPERATION INSTRUCTIONS
Page
2 - 6 6
/Image Acquisition
With types of dyeing of the specimen to observe, the Barrier Filters will be set
automatically to the optical path.
To change the Barrier Filter types, see to section 1-3-2-6, "Configuring the Filters" and
change by [Optical System Configuration] window.
Make the channels ready for image acquisition.
In the [Acquire] panel, make sure that the check box in the [Ch1]/[Ch2]/[Ch3]/[Ch4]
group boxes that show the dyeing methods are check-marked to indicate that the
corresponding channels are ready for image acquisition.
Ch2 check box
When observing a fluorescence image(s) simultaneously, also set the brightness of the
fluorescence image(s).
Adjust the brightness of the image of each channel by using the [PMT], [Offset] and
[Gain] LED sliders in the [Acquire] panel. For details, see section 2-2-1-4-9, "Adjusting
the Image Brightness".
Ch3 check box
Ch4 check box

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