Olympus FLUOVIEW FV1000 User Manual page 514

Software Setup/
Adding the filters
[Name of the Dye] text box
Enter the dyeing method name.
[Laser Type] drop-down list
Select the laser to be used.
MAINTENANCE
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4. In the [Dyes on your Samples] list, double-click "Double click here to make a new Dye".
The [Edit Dye] dialog box appears.
5. Enter the dyeing method name (CFP in this example) in the [Name of the Dye] text box.
6. Select the laser to be used (LD440 in this example) from the [Laser Type] drop-down
list.
TIP
It is required that the LD440 laser has been set as one of the laser types to be
used in advance. If "LD440" is not displayed, open the [Laser Equipment] panel
and check the [LD440] checkbox.
7. Enter the excitation wavelength value (480 in this example) in the [WaveLength 1] text
box in the [Excitation Wave Length] group box.
8. Enter the emission wavelength value (505 in this example) in the [WaveLength 1] text
box in the [Emission Wave Length] group box
TIP
In case of single excitation double emission, also enter an emission wavelength
in the [WaveLength 2] text box.
9. Click the <OK> button to close the [Edit Dye] dialog box.
10. Click the <Save New Setup> button to close the [FLUOVIEW Setup] dialog box.
[Excitation Wave Length]
group box
Enter the excitation
wavelength.
[Emission Wave Length]
group box
Enter the emission
wavelength.
In case of single excitation
double emission, also enter a
wavelength in the
[WaveLength 2] text box.