Olympus FLUOVIEW FV1000 User Manual page 77

The formula of resolution of Z is as follows;
FWHM : Resolution of Z
L : The length of a diagonal line of Square Pinhole
NA = nSINθ :NA of Objective lens
MG : The objective Magnification
N : The refractive index of a medium
Air : 1.0
Water : 1.3
Oil : 1.5
X = L/(1.22*3.8*λ*MG/NA)
The case where the variable x is as follows; 0 <= x < 2.0
FWHM = (2λ*ZPSF)/(4n(1-COSθ))
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ZPSF = -5.5158*10 x + 2.0877*10
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6.9038*10
The case where the variable x is as follows; 2.0 <= x
FWHM = (2λ*ZPSF)/(4n(1-COSθ))
ZPSF = 0.6164x+0.1283
Getting Started FLUOVIEW
One Point!
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x - 2.1172*10 x + 2.0864*10
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x - 2.2669*10 x +
OPERATION INSTRUCTIONS
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