Olympus FLUOVIEW FV1000 User Manual page 109

9 Adjusting the image brightness
Clicking this button
allows fine adjustment
of the value.
Clicking this field allows
the value to be changed
on a large scale.
The ND value which are
usually used are
displayed in green.
Clicking the <+> or <->
button or the field
displays the set value.
The set value can be
varied by direct input
from the keyboard.
PMT Voltage
Offset
Gain
When the acquired image is dark or the observation targets are hardly visible, increase
PMT voltage to improve the sensitivity. If the image resulting from this becomes too bright,
first try decreasing PMT voltage slightly. If the image background is still too bright, increase
Offset to darken the background. As this also reduces the brightness of the observation
target, increase Gain as required so that the observation target is clearly visible.
Gamma Correction provides you more brightened image even if it was acquired with
darkness.
See "2-5-2-2 LUT Graph Editing by Gamma Correction " for details in this operation.
1. While observing the image in the [Live] panel, change the setting values of the PMT
voltage[PMT], Offset[Offset] and Gain[Gain] in the [Acquire] panel. The functions of
these parameters are as described below.
Increasing this value improves the sensitivity.
However, too high a sensitivity makes image noise noticeable.
If sufficient brightness cannot be obtained by setting the PMT voltage to
800 V, leave it as it is and increase Gain. This will usually provide a better
result than using a PMT voltage over 800 V.
Darkens the image at the ratio set during image acquisition.
(This value can be set independently from the Gain.)
Brightens the image at the ratio set during image acquisition.
(This value can be set independently from the Offset.)
APPLIED OPERATIONS
For example...
/Image Acquisition
OPERATION INSTRUCTIONS
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