Olympus Fluoview FV1000 Manuals

Manuals and User Guides for Olympus Fluoview FV1000. We have 8 Olympus Fluoview FV1000 manuals available for free PDF download: User Manual, Overview, Short Instructions, Manuallines

Olympus Fluoview FV1000 User Manual (540 pages)

Confocal laser scanning biological microscope

Brand: Olympus | Category: Microscope | Size: 15.94 MB

Fluoview Manual Configuration

4
- Table of Contents
  4
Getting Started Fluoview 1-1

13
- Basic Operations
  23
- Getting Started FLUOVIEW
  23
- Microscope
  23
- General Mouse Operation Procedures
  29
- Names of Major Panel and Window Controls and Their Functions
  30
- Outline of LSM Observation Procedures
  31
- Turning Power on
  33
- Combination with BX
  34
- Focusing on the Specimen
  34
- Combination with IX81 FVF
  36
- Combination with Upright Microscope (BX)
  38
- Setting the LSM Light Path
  38
- Combination with Inverted Microscope (IX81 FVF)
  40
- Selecting the Dyeing Method
  42
- Selecting the Filters
  45
- Setting the ND Filters
  48
- Setting the Objective Magnification
  49
- Setting the Observation Condition
  49
- Setting the Channels
  50
- Setting the Zoom Ratio to 1X
  50
- Setting the Highest Scan Speed
  51
- Repeated Scanning Operation
  52
- Setting the XY Observation Mode
  52
- Setting the Cross-Section to be Observed
  53
- Setting a Lower Scan Speed
  54
- Setting the Area to be Observed
  54
- Acquiring Image
  55
- Stopping Repeated Scanning
  55
- Saving Image
  56
- Exiting from the Software, Turning Power off
  57
- Function Help
  58
- Online Help
  58
- Microscope Help
  59
- Configuring the Microscope
  62
- Parfocality Correction and Jog Sensitivity Adjustment
  68
- Configuring the Filters (When Using a Filter System)
  74
- Configuring the Filters (When Using a Spectral Detecting System)
  78
- Setting the C.A. Diameters
  81
- Applied Operations
  85
- General Operation Procedure
  85
- Image Acquisition Procedure (Section (A))
  87
- Image Acquisition Procedure in an Observation Mode (Section (B))
  88
- Examples of Operation Procedures
  89
- Image Acquisition
  91
- Image Acquisition in XY Observation Mode
  92
- Configuring the Microscope
  93
- Setting the Filters
  99
- Setting the ND Filters
  101
- Setting the Observation Condition
  103
- Acquiring Image
  112
- Acquiring Image in Accumulation Mode
  113
- Saving the Acquired Image in File
  117
- Image Acquisition in Other Observation Modes
  118
- XZ Observation Mode
  118
- XT Observation Mode
  122
- XZT Observation Mode
  124
- XYZ Observation Mode
  130
- XYT Observation Mode
  135
- XYZT Observation Mode
  139
- Differences in Image Acquisition Method between Fluorescent and Transmitted
  145
- Images
  145
- Monochrome Image
  145
- Dual-Fluorochrome Image
  148
- Transmitted Image
  151
- Image Acquisition by Rotating It (Rotation Scan)
  155
- Image Acquisition of Only the Rectangular Position (Clip Scan)
  156
- Image Acquisition by Magnifying the Rectangular Position (Zoom-In Scan)2-75
  159
- High-Speed Image Acquisition
  162
- Image Acquisition to Prevent Crosstalk between Fluorescence (Sequential Scan)
  163
- Virtual Channel Function
  166
- Image Acquisition of a Line at Desired Angle
  172
- Display the Change of Image Intensity (Point Scan)
  173
- Image Acquisition on Desired Line (XZ, XT or XZT Observation)
  178
- Image Acquisition in the Laser Excitation Mode
  181
- Making REX Mask File
  182
- Example of FRAP Experiment
  187
- Memory of Setting Information for Scanning Region
  196
- Notes for Image Acquisition
  196
- Saving, Opening and Shredding Images
  197
- Saving Images
  200
- Saving Images as a Series
  200
- Saving a Display
  202
- Saving Specified Area of Image
  204
- Saving Animation Images
  207
- File Types Available for Save
  209
- Opening Previously Saved Images
  216
- Shredding Images
  217
- Saving Comment Together with Image
  219
- Checking the Image Information/Acquisition Parameters
  222
- Saving the Image Information/Observation Condition
  226
- Saving the Region File
  229
- Saving/Reading the Region File
  229
- Reading the Region File
  231
- Protocol Processor
  234
- Starting the Protocol Processor
  235
- Editing the Protocol
  236
- Description of Setting Items
  237
- Command List
  244
- Protocol Repetition Processing
  249
- Input Supporting Function
  251
- Protocol Procedure Supporting Function
  253
- Saving the Protocol
  254
- Executing the Protocol
  256
- Loading a Protocol
  258
- Loading a Protocol of Previous Format
  259
- COM Communication Function
  261
- Example of Assembling a Protocol
  262
- Example of Setting Procedure of FRAP Observation
  262
- Example of Setting Procedure of XYT Observation for Hours
  271
- Pop-Up Menu
  279
- Restrictions of [PAPP] Setting
  280
- Restrictions with [Mode] and [Sub Mode]
  280
- Changing the Image Display Method
  282
- Displaying an Image in Simulated Colors
  282
- Editing the LUT (Look up Table)
  284
- LUT Graph Editing According to Colors
  284
- LUT Graph Editing by Gamma Correction
  286
- Switching the Displayed Channels (Ch1 - Ch5)
  287
- Displaying Images of Multiple Channels Simultaneously
  288
- Over and under Views, Single View)
  288
- Displaying Images Separately Per Channel
  289
- Views)
  289
- Displaying Merged Image of Multiple Channels (Single View)
  290
- Changing the Number of Divided Images
  291
- Increasing the Number of Divided Images
  291
- Decreasing the Number of Divided Images
  293
- Switching the Display Method of Multiple Images
  294
- Displaying Multiple Image Slices Together
  296
- Displaying Multiple Images Per Channel
  297
- Displaying Images of Two Channels Together
  299
- Displaying Time-Lapse Images
  300
- Displaying Multiple Multiple Sections Images
  301
- Displaying same Images in Different Display Methods
  301
- Displaying Different Images Together
  304
- Re-Arranging Images Using the same Display Method
  304
- Magnifying/Reducing an Image
  307
- Filtering
  309
- Image Processing
  309
- Contour Enhancement
  310
- Noise Reduction
  313
- Image Sharpening
  315
- DIC Correcting DIC Level Irregularities
  316
- Contrast Conversion
  318
- Image Addition
  321
- Mathematical Operations between Images
  321
- Image Multiplication
  324
- Image Subtraction
  324
- Image Division
  325
- NOT Image
  326
- Image and Image
  328
- Image or Image
  329
- Image XOR Image
  329
- Brightness Overlap Level between 2 Channels (Colocalization)
  330
- Annotation Mode
  331
- Colocalization for Series Image Data Set
  338
- Image Measurement
  339
- Appending Image (Append)
  342
- Appending Two Images
  342
- Appending Image from Several Image Data Set
  346
- Extract Image (Crop)
  351
- Image Analysis
  358
- Checking the Intensity of a Specific Part
  359
- Intensity Values on a Line (Line Profile)
  359
- Intensity Values on a Planar Region (Bird's Eye View)
  362
- Checking the Intensity Distribution of a Specific Part
  367
- Intensity Distribution on a Line (Histogram)
  367
- Intensity Distribution on a Planar Region (Histogram)
  368
- Area Measurement
  370
- Image Measurement
  370
- Length Measurement
  370
- Measuring the Change in Mean Value of Intensity
  371
- Measuring the Change in Integrated Intensity
  377
- Building an Image from a Different Viewpoint
  379
- Building Extended Focus Image from XYZ Image
  379
- Display Switching to Built Image
  379
- Turning Built Image into Single Image
  382
- Turning Built Image into Time Series Image
  384
- Building Line Images to be Viewed in Z Direction
  385
- Viewing 3D Image
  389
- Successive Display of Images
  391
- Changing the Successive Display Speed
  392
- Changing the Successive Image Display Position
  393
- Animation
  394
- Building Stereo 3D Images
  396
- Building a 3D Image to be Viewed through Color (Red/Green) Eyeglasses2-314
  398
- Displaying Images Successively
  400
- Displaying Images Together
  400
- Viewing Images Following the Progress of Time
  400
- Transferring Analysis Data to Another Application
  403
- Transferring Data to Another Application
  403
- Transferring the Plot Image of Analysis Data to Another Application
  406
- Transferring Image Data to Another Application (Paint, Etc.)
  407
- Entering Comment in Image
  408
- Writing Characters in Image
  408
- Displaying the Image Intensity
  410
- Displaying the X-Coordinate/Y-Coordinate of the Image
  411
- Drawing a Figure in Image
  412
- Drawing a Scale in Image
  413
- Drawing an Arrow in Image
  414
- Drawing Color Bars in Image
  415
- Deleting Comment
  416
- Moving Comment
  417
- Changing the Comment Size
  418
- Changing the Comment Color
  419
- Changing the Comment Font
  420
- Image Output at Printer
  421
- Merger/Extraction of Image Channels
  422
- Setting the Range of Multiple Image Slices
  422
- Merging Image Channels
  423
- Extracting Channels from Image
  428
- Changing the Chart Display Method
  432
- Chart] Panel
  432
- Series] Panel
  440
- Pop-Up Menus
  443
- Appendix A List of Hot Keys
  447
- Appendix B Glossary
  453
- Appendix C USER REGISTRATION of FV1000
  457
- Appendix C-1 User Registration
  457
- Appendix C-2 Logging into the FV1000
  460
- Appendix C-3 Deleting a User
  461
- Appendix D Change of Default Folder for [File I/O] Panel
  465
- Appendix E List of Functions in the [Active Overlays] Dialog Box
  471
- Appendix E-1 Coordinate Position Data
  471
- Appendix E-1-1 X-Coordinate
  471
- Appendix E-1-2 Y-Coordinate
  472
- Animation
  473
- Appendix E-1-3 Other
  473
- Position
  473
- Appendix E-2 Intensity Data
  474
- Appendix E-3 Other
  475
- Appendix E-3-1 Channel Number
  475
- Appendix E-3-2 Objective Power
  475
- Appendix E-3-3 Date of Image Capturing
  475
- Appendix E-3-4 Time of Image Capturing
  475
- Appendix E-3-5 Image File Name
  475
- Allocation
  477
- Appendix F Hand Switch and Microscope Frame Function
  477
- Appendix F Hand Switch and Microscope Frame Function Allocation
  477
- Appendix F-1 Hand Switch Functions
  477
- Appendix F-1-1 BX/BXWI
  477
- Appendix F-2 Microscope Frame Functions
  479
- Appendix F-2-1 BX
  479
- Appendix F-2-3 Focus Adjustment Knob
  481
- Maintenance
  483
Software Setup 1-1

485
- Re-Setup or Updating of the Software
  487
- Software Setup
  487
- New Setup of the Software
  489
- Overall Setting of FLUOVIEW
  491
- Setting the System Configuration
  491
- Setting the [Microscope Control Panel]
  500
- Adding the Dyeing Method
  507
- Adding Filters
  510
- Adding the Filters
  510
- Setting the Scanning Unit
  511
- Adding a Dyeing Method
  513
- Associating a Detection Channel and Filter to the Dyeing Method
  515
- Laser Scanning Microscope
  519
- Maintenance of Major System Units
  519
- Auto Alignment Operation (Remote Switch)
  520
- UV-Ar Laser
  520
- Checking, Refilling and Replacing Water in Chiller
  521
- Setting the Confocal Aperture
  525
- Starting the FLUOVIEW Software
  526
- Displaying the [C.A. Adjustment] Dialog Box
  527
- Confocal Aperture Setting
  529
- Troubleshooting
  533

Olympus Fluoview FV1000 User Manual (186 pages)

LASER SCANNING BIOLOGICAL MICROSCOPE

Brand: Olympus | Category: Microscope | Size: 2.84 MB

Section 1

8
- Quick Start
  1
- Table of Contents
  8
- System Preparation
  11
- Image Analysis
  38
- Image Analysis
  39
- Closing the System
  55
Section 2

58
Section 3

100
- System Preparation
  103
  - Observation of Fluorescence Image
    104
  - Observation of Differential Interference Contrast Images
    105
  - Overview of Operation Panel for Image Acquisition
    106
  - Single Stain on XY Image
    107
  - Complement of Adjusting the Image
    110
  - Double Stain on XY Image
    112
  - Double Stain on XYZ Image
    115
  - Four Stain on XY Image
    117
  - Single Stain + DIC on XY Image
    120
  - Unmixing
    128
  - Reload the Image Conditions
    130
- Image Analysis
  131
- Image Analysis
  132
  - Edit the Image Color and Contrast
    138
  - The Image of Z Section
    139
  - Intensity Profile of each Z Sections
    140
  - Measure
    141
Section 4

146
- Line Intensity Profile on the 2D Image / Histogram
  146
- System Preparation
  149
  - Observation of Fluorescence Image
    150
  - Observation of Differential Interference Contrast Images
    151
  - Overview of Operation Panel for Image Acquisition
    152
  - Single Stain on XY Image
    153
  - Complement of Adjusting the Image
    156
  - Double Stain on XY Image
    158
  - Sequential Scan Line Sequential
    160
  - Double Stain on XYZ Image
    161
  - Four Stain on XY Image
    163
  - Single Stain + DIC on XY Image
    166
  - Reload the Image Conditions
    169
- Image Analysis
  170
- Image Analysis
  171
  - Edit the Image Color and Contrast
    177
  - The Image of Z Section
    178
  - Intensity Profile of each Z Sections
    179
  - Measure
    180
  - Line Series Analysis / Co-Localization
    182
- Closing the System
  183

Olympus Fluoview FV1000 User Manual (42 pages)

CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPE

Brand: Olympus | Category: Microscope | Size: 8.36 MB

Manual Configuration

4
- Table of Contents
  4
1 System Overview 1-1

9
- System Overview
  11
  - Principles
    11
  - Features of the FV1000
    12
  - Optical Path Diagram
    13
  - System Configuration
    14
    
    System Diagram
    14
    
    System Appearance and Functions
    15
1 Preparation for Operation 1-1

19

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