Olympus FLUOVIEW FV1000 User Manual page 154

Confocal laser scanning biological microscope
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APPLIED OPERATIONS
Ch1 check box
OPERATION INSTRUCTIONS
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/Image Acquisition
When observing a fluorescence image simultaneously, set the required channel ready
for acquisition of fluorescence image.
Make sure that the check box showing the dyeing method in the [Ch1]/
[Ch2]/[Ch3]/[Ch4] group box is check-marked to indicate that the corresponding
channel is ready for image acquisition.
Ch2 check box
Set the brightness of the transmitted image.
Please use PMT, Offset and Gain LED sliders in [Ch5] group box to adjust the
brightness of the transmitted image. For details, see section 2-2-1-4-9, "Adjusting the
Image Brightness" in the [OPERATION] volume.
When observing a fluorescence image(s) simultaneously, also set the brightness of the
fluorescence image(s).
Adjust the brightness of the image of the transmitted image acquisition channel(s) by
using the [PMT], [Offset] and [Gain] LED sliders in its group box in the [Acquire] panel.
For details, see section 2-2-1-4-9, "Adjusting the Image Brightness" in the
[OPERATION] volume.
Ch3 check box
Ch4 check box

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