Olympus FLUOVIEW FV1000 User Manual page 275

Confocal laser scanning biological microscope
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Set each Track as sown below;
Setting Track 1
Acquire nine images in XYT observation.
1.
Select "New mode"-"XYT" in [Mode] of [Track].
2.
Select "Normal" in [Start by] of [Track].
3.
Enter "9" to [Condition] of [Track].
4.
Select "Middle" in [Scan Speed].
5.
Enter the laser intensity value for image acquisition to [Laser Intensity].
6.
Enter the PMT value of each channel to [PMT Voltage].
7.
Enter image file name to be stored to [Save FileName.].
8.
Enter a comment in [Comment] if necessary.
TIP
The actual image file name becomes using the name of [Image File Name] that
followed by the acquisition order number.
Assign the image file name in the [Image File Name] text box area before
protocol starts.
Setting Track 2
Specifies N variable and designate range to be repeated. Protocol continues between the
next Track of "For" statement and the Track just before "Loop" statement.
1.
Select "For" in [Mode] of [Track].
2.
Enter "N = 1 To 7" to [Condition] of [Track].
3.
It is not necessary to enter the values in other cells.
APPLIED OPERATIONS
OPERATION INSTRUCTIONS
Page
/Protocol processor
2 - 1 9 1

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