Olympus FLUOVIEW FV1000 User Manual page 531

7. Rotate the focus adjustment knob of the microscope to bring the mirror surface
into focus. The focal position is the position where the image is bright and
displays scratches on the mirror surface most clearly.
8. While checking the repeated scanning image on the image window, adjust
the confocal aperture to the position that provides the brightest image by using
the arrow buttons on the center of the [Control] group box in the [C.A.
Adjustment] dialog box and the [Current] text box in the [Position] group box.
Click to move the confocal
aperture position by 40 µm.
The orientation of the
indicates the direction.
Click to move the confocal
aperture position to the saved position.
TIP
The confocal aperture position can be moved between –750 and 750 µm.
9. Click the <200> button in the [Set C.A. Diameter] group box.
(For the display, see step 6.)
10. Rotate the focus adjustment knob of the microscope to bring the mirror surface
into focus. The focal position is the position where the image is bright and
displays scratches on the mirror surface most clearly.
11. Perform the confocal aperture adjustment in step 8 so that the image is brightest.
12. Click the <80> button in the [Set C.A. Diameter] group box.
(For the display, see step 6.)
13. Rotate the focus adjustment knob of the microscope to bring the mirror surface
into focus. The focal position is the position where the image is bright and
displays scratches on the mirror surface most clearly.
Setting the Confocal Aperture/
triangle
Confocal Aperture Setting
Click to move the
confocal aperture position by
8 µm.
The orientation of the
triangle indicates the direction.
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