Olympus FLUOVIEW FV1000 User Manual page 532

Setting the Confocal Aperture/
MAINTENANCE
3 - 8
Page
Confocal Aperture Setting
14. Perform the confocal aperture adjustment in step 8.
The correct confocal aperture position is the position with which the bright part
comes on the center of the screen.
The bright part may sometimes move across the stop edges of the image
window during the focal position adjustment. Adjust the focus and confocal
aperture position alternate for a few cycles.
15. Click the <Save Position> button to save the confocal aperture position.
TIP
The saved position becomes the initial setting for the confocal aperture at
the moment the FLUOVIEW software is started. This position is set
from immediately after the save operation.
16. Click the <STOP SCAN> button in the [Acquire] panel to stop image scanning.
17. Adjust the confocal aperture positions of other combinations of excitation DM
and spectral BS, which have not been set yet, by repeating steps 1. to 16.
18. Click the <Close> button to close the [C.A. Adjustment] dialog box.