Olympus FLUOVIEW FV1000 User Manual page 193

7 Acquiring Data
<XYT> button
The experiment data before and after photobleaching of certain region can be obtained after
performing an ordinary image acquisition in the first scan, an image acquisition in the Bleach
mode in the second scan , and an ordinary image acquisition in the third scan. When the
REX mode is selected in the third scan, the data of the region other than the specified region
can not be obtained since the laser is irradiated only to the specified region. However, the
REX mode is effective to prevent fading of the obtained data.
1. Select the [Disabled] option button in the [AOTF] group box in the [Lasers] sub-panel.
[Disabled] option button
2. Click the <XYT> button to start image acquisition.
3. Select the [Bleach mode] option button in the [AOTF] group box in the [Lasers] sub-
panel.
[Bleach mode] option button
NOTE
Select the [Bleach mode] option button within 10 seconds before the next
scanning is started.
NOTE
In the TIME COURSE software (optional), when the REX mask file in the
REX or Bleach mode is changed while image scanning is performed
obtaining the real time graph, the laser is irradiated while the pop-up menu
is displayed. however, the real time graph is not plotted. Please be aware
this before using.
APPLIED OPERATIONS
OPERATION INSTRUCTIONS
Page
/Image Acquisition
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