Olympus FLUOVIEW FV1000 User Manual page 121

Confocal laser scanning biological microscope
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3 Setting the observation line
4 Setting the numbers of steps and acquired image slices
[Step Size] text box
[Slices] text box
5 Acquiring image
1.
A line is displayed on the image in the [Live] panel. Place the mouse pointer arrow on
the line and drag it to the position you want to observe.
1.
From the page tabs on the bottom right of the [Acquire] panel, select the [Z Stage]
sub-panel.
2.
The number of steps has already been set and displayed in the [Step Size] text box.
This number can be changed using the <
TIP
The number of steps in the [Step Size] text box has been calculated by the
system so that the depth scale of the acquired image is identical to the
horizontal scale.
NOTE
The number of steps calculated by the system may be erroneous unless
the XZ observation mode has been set previously.
TIP
The number of acquired images shown in the [Slices] text box can also be input
from the keyboard.
After setting [Start Z] (Z-direction scan start position) and [Stop Z] (Z-direction
scanning stop position), input the desired number of images in the [Slices] text
box. This automatically sets [Step Size] (number of steps).
1.
Click the <XZ> button in the [Acquire] panel.
The acquired image will be displayed in the [Live] panel.
APPLIED OPERATIONS
> or <
> button in the [Step Size] text box.
OPERATION INSTRUCTIONS
Page
/Image Acquisition
2 - 3 7

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