Olympus FLUOVIEW FV1000 User Manual page 388

APPLIED OPERATIONS
When building 3D image in Ver5.0 or subsequent version, pay attention to the following points.
When using XZ direction as reference, 3D image including images of YZ (upper right) and XY (lower left) can be built.
When using YZ direction as reference, 3D image including images of XY (upper right) and XZ (lower left) can be built.
In this case, the size of image in each direction may be different.
The XY display area in the 3D image is intended for life-size display of image pixel values. The X and Y resolution
values are not taken in consideration at this time.
However, when both of the following conditions are met, Z in the XZ and YZ display areas are displayed in a larger
size than the actual pixel value.
· Resolutions of X and Y are equal
· The resolution of Z is higher than that of X and Y.
Example: Rebuilding the 3D image of Sample_XYZ.tif based on its YZ image.
Left figure (Before building): Resolutions of X and Y are equal (both 0.26) and the resolution of X (0.5) is higher
Right figure (After building): Resolutions of X(0.26) and Z(0.5) are changed with each other. As a result, resolutions
Z-direction display size is approximately
doubled from the actual number of pixels.
Number of pixels and resolution (um) in
each direction
OPERATION INSTRUCTIONS
Page
2 - 3 0 4
/Building an Image from a Different Viewpoint
than that of Y, Z is displayed in a larger size than the actual number of pixels.
(Since the resolution of Z is about twice that of X and Y, the display in the Z direction is
approximately doubled compared to the actual size.)
X and Y are unequal so the image is displayed in the actual pixel size.
247
214
16 times Appox.2
X : 214(pixel) X : 0.26(um)
Y : 247 Y : 0.26
Z : 16 Z : 0.5
One Point!
YZ image displayed in the actual pixel
size.
Rebuilding→
247
16
Number of pixels and resolution (um) in
each direction
214
X : 16 X : 0.5
Y : 247 Y : 0.26
Z : 214 Z : 0.26