Olympus FLUOVIEW FV1000 User Manual page 340

APPLIED OPERATIONS
OPERATION INSTRUCTIONS
Page
2 - 2 5 6
/Image Processing
· Pearson's Coefficient :
In case that the value to be acquired is defined as
∑
− ⋅
( F F )
, 1 i , 1 i
=
i
r
∑ ∑
− 2 ⋅
( F F )
, 1 i , 1 i
i
However, legends used for image measurement are as follows:
F
: Brightness of wavelength
, 1 i
i
-th pixel
1
∑
=
F F
: Average brightness of wavelength
, 1 i , 1 i
N
i
λ
brightness of wavelength
· Overlap :
In case that the value to be acquired is defined as
∑
⋅
F F
, 1 i , 2 i
=
i
r
∑ ∑
2
⋅
F F
, 1 i , 2 i
i i
· Overlap Index #1 :
In case that the value to be acquired is defined as
∑
⋅
F F
, 1 i , 2 i
=
i
k
∑
1
2
F
, 1 i
i
· Overlap Index #2 :
In case that the value to be acquired is defined as
∑
⋅
F F
, 1 i , 2 i
=
i
k
∑
2
2
F
, 2 i
i
· Colocalization Index #1 :
In case that the value to be acquired is defined as
∑
F
, 1 i
= ∈
i A
m
∑
1
F
, 1 i
i
i ∈
A
However, should be
threshold value.
· Colocalization Index #2 :
r
−
( F F )
, 2 i , 2 i
− 2
( F F )
, 2 i , 2 i
i
λ
i
at -th pixel,
1
2
r
2
k
k
m
i
-th pixel that belongs to aggregation A that is greater than
,
F
: Brightness of wavelength
, 2 i
1
∑
λ
=
F F
,
1 , 2 i
N
i
,
,
1
,
2
,
1
λ
at
2
: Average
, 2 i