Olympus FLUOVIEW FV1000 User Manual page 195

Confocal laser scanning biological microscope

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The image of the specimen

is fading only where the

laser is irradiated.

Start the third scan in 10 seconds after the second scan is performed.

(The [Disabled] option button is selected.)

Start the forth and subsequent scan in 10 seconds after the third scan is performed.

The image of the photobleached specimen is acquired.

When performing the forth or subsequent scan, be sure to switch the [REX

mode] and [Bleach mode] between scans.

In the TIME COURSE software (optional), when the REX mask file in the

REX or Bleach mode is changed while image scanning is performed

obtaining the real time graph, the laser is irradiated while the pop-up menu

is displayed, however, the real time graph is not plotted. Please be aware

this before using.

APPLIED OPERATIONS

OPERATION INSTRUCTIONS

/Image Acquisition

Chapters

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